TIMP-1 antibody | M03

Mouse anti Human TIMP-1

Product Type
Monoclonal Antibody
Clone
M03
Isotype
IgG1
Specificity
TIMP-1

Product Code Applications Pack Size List Price Your Price Qty
MCA6278GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human TIMP-1 antibody, clone M03, recognizes Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) also known as metalloproteinase inhibitor 1, EPA and fibroblast collagenase inhibitor.

TIMP-1 is a member of the TIMP family of proteins that assist in regulating the activity of matrix metalloproteinases (MMPs) and it is mainly expressed by macrophages and fibroblasts. TIMP proteins are specific endogenous inhibitors that bind to the active site of MMPs in a stoichiometric 1:1 molar ratio, thereby blocking access to ECM substrates (Kapoor et al. 2016, Robert et al. 2016). The TIMP proteins including TIMP-1 have poor specificity for any particular MMP and each can inhibit multiple MMPs with differing efficacies (Cui et al. 2017).

The ratio of MMP and TIMP often determine the extent of ECM protein degradation and tissue remodeling. An imbalance of this ratio such as with decreased expression of TIMP can result in various pathological conditions, most notably, heart failure, osteoarthritis and cancer. Low levels of TIMP have also been associated with slow-to-heal wounds and venous wounds (Cui et al. 2017).

The purified Mouse anti Human TIMP-1 antibody, clone M03 (MCA62678GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human TIMP-1 antibody, clone M05 (MCA6279B) as the detection antibody.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
E. coli derived recombinant human full length TIMP-1 (Cys24-Ala207)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
ELISA Matched Pair(s)
ELISA Matched Pair-Detection Antibody: MCA6279B
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human TIMP-1:Biotin MCA6279B E 0.1 mg loader
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Description Mouse anti Human TIMP-1:Biotin

Synonyms
Metalloproteinase Inhibitor 1
UniProt
P01033
Entrez Gene
TIMP1
GO Terms
GO:0005515 protein binding
GO:0002576 platelet degranulation
GO:0046872 metal ion binding
GO:0005578 proteinaceous extracellular matrix
GO:0008191 metalloendopeptidase inhibitor activity
GO:0008284 positive regulation of cell proliferation
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0043249 erythrocyte maturation
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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