TIMP-1 antibody | M05

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Mouse anti Human TIMP-1:Biotin

Product Type
Monoclonal Antibody
Clone
M05
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6279B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human TIMP-1 antibody, clone M05, recognizes Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) also known as metalloproteinase inhibitor 1, EPA and fibroblast collagenase inhibitor.

TIMP-1 is a member of the TIMP family of proteins that assist in regulating the activity of matrix metalloproteinases (MMPs) and it is mainly expressed by macrophages and fibroblasts. TIMP proteins are specific endogenous inhibitors that bind to the active site of MMPs in a stoichiometric 1:1 molar ratio, thereby blocking access to ECM substrates (Kapoor et al. 2016, Robert et al. 2016). The TIMP proteins including TIMP-1 have poor specificity for any particular MMP and each can inhibit multiple MMPs with differing efficacies (Cui et al. 2017).

The ratio of MMP and TIMP often determine the extent of ECM protein degradation and tissue remodeling. An imbalance of this ratio such as with decreased expression of TIMP can result in various pathological conditions, most notably, heart failure, osteoarthritis and cancer. Low levels of TIMP have also been associated with slow-to-heal wounds and venous wounds (Cui et al. 2017).

The biotinylated Mouse anti Human TIMP-1 antibody, clone M05 (MCA62779B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human TIMP-1 antibody, clone M03 (MCA6278GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
E. coli derived recombinant human full length TIMP-1 (Cys24-Ala207)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P01033
Entrez Gene
TIMP1
GO Terms
GO:0005515 protein binding
GO:0002576 platelet degranulation
GO:0046872 metal ion binding
GO:0005578 proteinaceous extracellular matrix
GO:0008191 metalloendopeptidase inhibitor activity
GO:0008284 positive regulation of cell proliferation
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0043249 erythrocyte maturation
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
Regulatory
For research purposes only

Applications of TIMP-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human TIMP-1 MCA6278GA E 0.1 mg loader

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