Protein Gene Product 9.5 antibody | 13C4

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Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 1

Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 2

Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 3

Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 1 — Rat brain lysate probed with Mouse anti Human Protein Gene Product 9.5 antibody, clone 13C4 (**7863-2004**) by western blotting

Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 2 — **Published customer image:**
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (**7863-2004**) used for the detection of PGP9.5 immunoreactive nerve fibers in porcine gastric mucosa by immunofluorescence.
**Image caption:**
Tissue sampling and cell measurements. (a) Picture presenting the interior surface of the "experimental animal" stomach, exposed by cutting along the greater curvature of the stomach. Ulcers are indicated by red arrows. (b) Microphotograph showing Haematoxylin and Eosin staining of the gastric ulcer margin. The gastric mucosa shows a defect (red ellipse with the inscription “Ulcer” and arrows) that extends into the deep muscular layers. (c) Diagram showing the method of tissue sampling. The tracer injection site is indicated by the violet arrows. The red vertical stripes symbolize the subsequent 0.5 cm thick transverse sections taken in specific intervals (section I—1.5 cm; section II—3.5cm; section III—5.5 cm; section IV—7.5cm) starting from the pyloric sphincter (section 0—blue vertical stripe). Subsequently, each of these sections (I, II, III, IV) was cut into 20 μm thick cryostat consecutive microscopic slices for future processing. The red circle symbolizes the localization of ulcer induced in experimental animals. (d, e, f) Photomicrographs showing: (d) bundles of PGP-immunoreactive fibers (arrowheads) and perikarya (arrows). Double arrow points to the perikaryon which was fast blue (FB) positive [the perimeter (l) and area (A) of the cell body are included]; (e) fast blue (FB) positive cell body (double arrow) with lines indicating its dimensions; (f) the image formed by merging both channels (green: PGP 9.5 and blue: FB).

From: Zalecki M (2015) The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)—Neuronal Tracing Studies.
PLoS ONE 10(5): e0126958.

Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 3 — **Published customer image:**
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 used for the detection of PGP9.5 immunoreactive nerve fibers in porcine descending colon by immunofluorescence.
**Image caption:**
Distribution pattern of nervous structures immunoreactive to protein gene-product 9.5 (PGP 9.5)—used as a panneronal marker and calcitonin gene-related peptide (CGRP) in the wall of porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration; I—myenteric plexus; II—outer submucous plexus; III—inner submucous plexus. CGRP-positive neurons (I,II,III) are indicated by arrows. Images I, II, and III are composites of merged images taken separately from green (PGP 9.5) and red (CGRP) fluorescent channels.

From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.

Mouse anti Human Protein Gene Product 9.5

Product Type: Monoclonal Antibody
Clone: 13C4
Isotype: IgG2a

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
7863-2004	E IF P* WB			0.2 ml

Mouse anti Human protein gene product 9.5 antibody, clone 13C4 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway.

This product stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES. Also stains neuroendocrine cells in human adult gut (unlike 31A3).

Clones 31A3 and 13C4 each recognise a different epitope towards the N-terminus of the protein.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Guinea Pig
Pig
Beaver

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Native, from brain

Approx. Protein Concentrations

1 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P09936 Related reagents
Entrez Gene: UCHL1 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0004197 cysteine-type endopeptidase activity; GO:0004221 ubiquitin thiolesterase activity; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0006511 ubiquitin-dependent protein catabolic process; GO:0008242 omega peptidase activity; GO:0016579 protein deubiquitination; GO:0016874 ligase activity; GO:0031694 alpha-2A adrenergic receptor binding; GO:0043130 ubiquitin binding; GO:0043407 negative regulation of MAP kinase activity
Regulatory: For research purposes only

Applications of Protein Gene Product 9.5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin ¹		1/200
Western Blotting	1/100	1/1000

¹ Antigen is stable in formalin fixed paraffin embedded sections, however we recommend fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding. Can be used without acid/ethanol fixation if the sections are subjected to microwave treatment in citrate buffer by standard methods.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Immunofluorescence

Product Specific References

References for Protein Gene Product 9.5 antibody

Wilson, P.O. et al. (1988) The immunolocalization of protein gene product 9.5 using rabbit polyclonal and mouse monoclonal antibodies.
Br. J. Exp. Pathol. 69: 91-104.
Kotani, T. et al. (2010) Expression of PTPRO in the interneurons of adult mouse olfactory bulb.
J Comp Neurol. 518: 119-36.
Buels, K.S. et al. (2012) Non-bronchodilating mechanisms of tiotropium prevent airway hyperreactivity in a guinea-pig model of allergic asthma.
Br J Pharmacol. 165: 1501-14.
Sasaki, H. et al. (2001) Expression of the protein gene product 9.5, PGP9.5, is correlated with T-status in non-small cell lung cancer.
Jpn J Clin Oncol. 31: 532-5.
Burliński, P.J. (2012) Inflammation- and axotomy-induced changes in cocaine- and amphetamine-regulated transcript peptide-like immunoreactive (CART-LI) nervous structures in the porcine descending colon.
Pol J Vet Sci. 15 (3): 517-24.
Bulc, M. et al. (2012) Immunohistochemical characterization of the porcine nodose ganglion.
Acta Histochem. pii: S0065-1281(12)00142-0.
Dudek, A. et al. (2012) Immunohistochemical characterization of neurons in the vestibular ganglion (Scarpa's ganglion) of the pig.
Pol J Vet Sci.15: 499-507.
Zalecki, M. (2015) The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)-Neuronal Tracing Studies.
PLoS One. 10 (5): e0126958.
Akazawa, N. et al. (2014) Neuroendocrine carcinoma of the esophagus: clinicopathologic study of 10 cases and verification of the diagnostic utility of mASH1, NeuroD1, and PGP9.5
Esophagus. 11 (4): 245-257.
Cooke, H.J. et al. (1999) Activation of neuronal adenosine A1 receptors suppresses secretory reflexes in the guinea pig colon.
Am J Physiol. 276 (2 Pt 1): G451-62.
Godlewski J & Pidsudko Z (2012) Characteristic of galaninergic components of the enteric nervous system in the cancer invasion of human large intestine.
Ann Anat. 194 (4): 368-72.
Kaleczyc, J. et al. (2007) The distribution and chemical coding of intramural neurons supplying the porcine stomach - the study on normal pigs and on animals suffering from swine dysentery.
Anat Histol Embryol. 36 (3): 186-93.
Komori, N. et al. (2003) Presence of beta-arrestin-1 immunoreactivity in the cutaneous nerve fibers of rat glabrous skin.
Brain Res. 988 (1-2): 121-9.
Pidsudko, Z. et al. (2008) Distribution and chemical coding of intramural neurons in the porcine ileum during proliferative enteropathy.
J Comp Pathol. 138 (1): 23-31.
Pidsudko, Z. (2013) Immunohistochemical characteristics and distribution of neurons in the intramural ganglia supplying the urinary bladder in the male pig.
Pol J Vet Sci. 16 (4): 629-38.
Sienkiewicz, W. et al. (2000) Has active immunization against gonadotrophin-releasing hormone any effect on testis innervation in the pig? An immunohistochemical study.
Anat Histol Embryol. 29 (4): 247-54.
Zalecki, M. et al. (2019) Enteric nervous system in the European beaver (Castor fiber Pol J Vet Sci. 22 (1): 101-7.

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Rat brain lysate probed with Mouse anti Human Protein Gene Product 9.5 antibody, clone 13C4 (7863-2004) by western blotting

Published customer image:
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used for the detection of PGP9.5 immunoreactive nerve fibers in porcine gastric mucosa by immunofluorescence.
Image caption:
Tissue sampling and cell measurements. (a) Picture presenting the interior surface of the "experimental animal" stomach, exposed by cutting along the greater curvature of the stomach. Ulcers are indicated by red arrows. (b) Microphotograph showing Haematoxylin and Eosin staining of the gastric ulcer margin. The gastric mucosa shows a defect (red ellipse with the inscription “Ulcer” and arrows) that extends into the deep muscular layers. (c) Diagram showing the method of tissue sampling. The tracer injection site is indicated by the violet arrows. The red vertical stripes symbolize the subsequent 0.5 cm thick transverse sections taken in specific intervals (section I—1.5 cm; section II—3.5cm; section III—5.5 cm; section IV—7.5cm) starting from the pyloric sphincter (section 0—blue vertical stripe). Subsequently, each of these sections (I, II, III, IV) was cut into 20 μm thick cryostat consecutive microscopic slices for future processing. The red circle symbolizes the localization of ulcer induced in experimental animals. (d, e, f) Photomicrographs showing: (d) bundles of PGP-immunoreactive fibers (arrowheads) and perikarya (arrows). Double arrow points to the perikaryon which was fast blue (FB) positive [the perimeter (l) and area (A) of the cell body are included]; (e) fast blue (FB) positive cell body (double arrow) with lines indicating its dimensions; (f) the image formed by merging both channels (green: PGP 9.5 and blue: FB).

From: Zalecki M (2015) The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)—Neuronal Tracing Studies.
PLoS ONE 10(5): e0126958.

Published customer image:
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 used for the detection of PGP9.5 immunoreactive nerve fibers in porcine descending colon by immunofluorescence.
Image caption:
Distribution pattern of nervous structures immunoreactive to protein gene-product 9.5 (PGP 9.5)—used as a panneronal marker and calcitonin gene-related peptide (CGRP) in the wall of porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration; I—myenteric plexus; II—outer submucous plexus; III—inner submucous plexus. CGRP-positive neurons (I,II,III) are indicated by arrows. Images I, II, and III are composites of merged images taken separately from green (PGP 9.5) and red (CGRP) fluorescent channels.

From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.

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