Protein Gene Product 9.5 antibody | 13C4
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used for the detection of PGP9.5 immunoreactive nerve fibers in porcine gastric mucosa by immunofluorescence.
Image caption:
Tissue sampling and cell measurements. (a) Picture presenting the interior surface of the "experimental animal" stomach, exposed by cutting along the greater curvature of the stomach. Ulcers are indicated by red arrows. (b) Microphotograph showing Haematoxylin and Eosin staining of the gastric ulcer margin. The gastric mucosa shows a defect (red ellipse with the inscription “Ulcer” and arrows) that extends into the deep muscular layers. (c) Diagram showing the method of tissue sampling. The tracer injection site is indicated by the violet arrows. The red vertical stripes symbolize the subsequent 0.5 cm thick transverse sections taken in specific intervals (section I—1.5 cm; section II—3.5cm; section III—5.5 cm; section IV—7.5cm) starting from the pyloric sphincter (section 0—blue vertical stripe). Subsequently, each of these sections (I, II, III, IV) was cut into 20 μm thick cryostat consecutive microscopic slices for future processing. The red circle symbolizes the localization of ulcer induced in experimental animals. (d, e, f) Photomicrographs showing: (d) bundles of PGP-immunoreactive fibers (arrowheads) and perikarya (arrows). Double arrow points to the perikaryon which was fast blue (FB) positive [the perimeter (l) and area (A) of the cell body are included]; (e) fast blue (FB) positive cell body (double arrow) with lines indicating its dimensions; (f) the image formed by merging both channels (green: PGP 9.5 and blue: FB).
From: Zalecki, M. (2015)
The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)—Neuronal Tracing Studies.
PLoS ONE 10(5): e0126958.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 used for the detection of PGP9.5 immunoreactive nerve fibers in porcine descending colon by immunofluorescence.
Image caption:
Distribution pattern of nervous structures immunoreactive to protein gene-product 9.5 (PGP 9.5)—used as a panneronal marker and calcitonin gene-related peptide (CGRP) in the wall of porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration; I—myenteric plexus; II—outer submucous plexus; III—inner submucous plexus. CGRP-positive neurons (I,II,III) are indicated by arrows. Images I, II, and III are composites of merged images taken separately from green (PGP 9.5) and red (CGRP) fluorescent channels.
From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used for the identification of PGP9.5 expressing cells in donkey testes by immunofluorescence.
Image caption:
Expression of PGP9.5 in Leydig cells of pre- and post-pubertal donkeys. Testicular tissue stained with IgG showed no immunolabeling (a,e). The expression of PGP9.5 was observed in the testes of pre-pubertal (b–d) and post-pubertal (f–h) donkeys. The white arrowheads indicate Leydig cells stained with PGP9.5. The yellow arrowheads indicate Leydig cells without PGP9.5 staining. The white bar = 50 μm.
From: Choi Y, Jung Y, Kim S, Kim J, Jung H, Yoon M.
Stage-Dependent Expression of Protein Gene Product 9.5 in Donkey Testes.
Animals (Basel). 2020 Nov 20;10(11):E2169.
doi: 10.3390/ani10112169.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used for the identification of PGP9.5 expressing cells in donkey testes by western blotting.
Image caption:
Cross-reactivity of protein gene product (PGP)9.5 in donkey testes. The PGP9.5 protein band appeared at the molecular size of approximately 27 kDa. The β-actin band was also detected at approximately 45 kDa in donkey testes. The negative control lane was treated with normal mouse IgG with the same concentration as the primary antibody and showed no bands.
From: Choi Y, Jung Y, Kim S, Kim J, Jung H, Yoon M.
Stage-Dependent Expression of Protein Gene Product 9.5 in Donkey Testes.
Animals (Basel). 2020 Nov 20;10(11):E2169.
doi: 10.3390/ani10112169.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human gene product 9.5 antibody, clone 13C4 (7863-0204) used to demonstrate neurons in porcine submucosal plexus by immunofluorescence.
Image caption:
Inner submucous plexus in control animals (a) and after BPA treatment in low (b) and high (c) doses, immunostained for protein gene product 9.5 (PGP 9.5—used here as a pan-neuronal marker) and other neuronal factors including substance P (SP) and vasoactive intestinal polypeptide (VIP). Neurons showing co-localization of PGP 9.5 and other neuronal factors are indicated with arrows.
From: Szymanska K, Makowska K, Gonkowski S.
The Influence of High and Low Doses of Bisphenol A (BPA) on the Enteric Nervous System of the Porcine Ileum.
Int J Mol Sci. 2018 Mar 20;19(3):917.
doi: 10.3390/ijms19030917.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti PGP9.5 antibody, clone 13C4 (7863-0204) used to demonstrate neurons in porcine submucosal plexus by immunofluorescence.
Image caption:
Outer submucous plexus in control animals (a) and after BPA treatment in low (b) and high (c) doses, immunostained for protein gene product 9.5 (PGP 9.5, a pan-neuronal marker) and other neuronal factors including substance P (SP) and vasoactive intestinal polypeptide (VIP). Neurons showing co-localization of PGP 9.5 and other neuronal factors are indicated with arrows.
From: Szymanska K, Makowska K, Gonkowski S.
The Influence of High and Low Doses of Bisphenol A (BPA) on the Enteric Nervous System of the Porcine Ileum.
Int J Mol Sci. 2018 Mar 20;19(3):917.
doi: 10.3390/ijms19030917.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Protein Gene Product 9.5 antibody, clone 13C4 (7863-2004) used to label porcine myenteric neurons in conjunction with substance P using Mouse anti substance P antibody, clone NC1/34 (8450-0505) by immunofluorescence.
Image caption:
Immunofluorescence findings of ENS neurons in the myenteric plexuses. Representative images of duodenum myenteric neurons immunopositive to SP, in physiological state (A), after low (B) and high (C) doses of acrylamide supplementation. (A-C) - neurons immunopositive to protein gene-product 9.5 (PGP9.5) - used as a panneuronal marker and SP; All photographs have been made by overlapping of green and red fluorescent channels (green for PGP 9.5 and red for SP). Neurons immunopositive to particular substance studied are indicated with arrows.
From: Palus K, Całka J.
Influence of Acrylamide Administration on the Neurochemical Characteristics of Enteric Nervous System (ENS) Neurons in the Porcine Duodenum.
Int J Mol Sci. 2019 Dec 18;21(1):15.
doi: 10.3390/ijms21010015.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Protein Gene Product 9.5 antibody, clone 13C4 (7863-2004) used to label porcine myenteric neurons in conjunction with substance P using Mouse anti substance P antibody, clone NC1/34 (8450-0505) by immunofluorescence.
Image caption:
Immunofluorescence findings of the ENS neurons in the outer submucous plexuses. Representative images of duodenum other submucous neurons immunopositive to SP in physiological state (A), after low (B) and high (C) doses of acrylamide supplementation. (A-C) - outer submucous neurons immunopositive to protein gene-product 9.5 (PGP9.5) - used as a panneuronal marker and SP; All photographs have been made by overlapping of green and red fluorescent channels (green for PGP 9.5 and red for SP). Neurons immunopositive to particular substance studied are indicated with arrows.
From: Palus K, Całka J.
Influence of Acrylamide Administration on the Neurochemical Characteristics of Enteric Nervous System (ENS) Neurons in the Porcine Duodenum.
Int J Mol Sci. 2019 Dec 18;21(1):15.
doi: 10.3390/ijms21010015.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Protein Gene Product 9.5 antibody, clone 13C4 (7863-2004) used to label porcine myenteric neurons in conjunction with substance P using Mouse anti substance P antibody, clone NC1/34 (8450-0505) by immunofluorescence.
Image caption:
Immunofluorescence findings of the ENS neurons in the inner submucous plexuses. Representative images of duodenum inner submucous neurons immunopositive to SP in physiological state (A), after low (B) and high (C) doses of acrylamide supplementation. (A-C) - inner submucous neurons immunopositive to protein gene-product 9.5 (PGP9.5) - used as a panneuronal marker and SP; All photographs have been made by overlapping of green and red fluorescent channels (green for PGP 9.5 and red for SP). Neurons immunopositive to particular substance studied are indicated with arrows.
From: Palus K, Całka J.
Influence of Acrylamide Administration on the Neurochemical Characteristics of Enteric Nervous System (ENS) Neurons in the Porcine Duodenum.
Int J Mol Sci. 2019 Dec 18;21(1):15.
doi: 10.3390/ijms21010015.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used to identify PGP9.5 exprressing perikarya in rat nodose ganglion sections by immunofluorescence.
Image caption:
Distribution and typical characteristics (shapes, immunofluorescence) of PGP 9.5/Gal-immunofluorescent perikarya within the longitudinal cross-sectional plane of the nodose ganglion in the control and experimental animals.
A set of microphotographs showing longitudinal cross-section of the representative inferior (nodose) vagal ganglion in the control (A) and experimental (B) animals, double immunostained with antibodies against PGP 9.5 (green channel) and galanin (red channel). The microphotographs present the overlap of both fluorescence channels. Figures (A) and (B) were created by combining a series of photomicrographs with the use of a confocal laser microscope tile scan function and present the entire cross-sectional surface of the ganglion. High magnifications of selected areas (dotted line border from the picture (A) and (B)) are presented in the pictures below (A-1, A-2, A-3, A-4—control animals; B-1, B-2, B-3, B-4—experimental animals). PGP 9.5/Gal-immunoreactive perikarya are marked with arrows. Double arrows point to perikarya characterized by medium-to-weak Gal-immunofluorescence. Gal-immunofluorescent neuronal fibers are marked with arrowheads. Scale bars are included in the pictures.
From: Zalecki M, Juranek J, Pidsudko Z, Mogielnicka-Brzozowska M, Kaleczyc J, Franke-Radowiecka A.
Inferior vagal ganglion galaninergic response to gastric ulcers.
PLoS One. 2020 Nov 23;15(11):e0242746.
doi: 10.1371/journal.pone.0242746.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used to identify PGP9.5 exprressing perikarya in rat nodose ganglion sections by immunofluorescence.
Image caption:
Characteristics (shapes, immunofluorescence) of PGP9.5/Gal-immunofluorescent vagal nodose perikarya occasionally observed in the control and experimental animals.
Microphotographs showing high magnifications of occasionally observed Gal-immunoreactive neurons (arrows) with large (A-1, A-3, A-3, C-1, C-2, C-3) and small (B-1, B-3, B-3, D-1, D-2, D-3) cell body sizes occurring in the vagal nodose ganglia in the control (A-1, A-3, A-3, B-1, B-3, B-3) and experimental (C-1, C-2, C-3, D-1, D-2, D-3) animals. A characteristic, clump-like Gal-immunofluorescence pattern is visible in some of the perikarya (A-1, A-3, A-3, C-1, C-2, C-3, D-1, D-2, D-3), while others are characterized by strong, evenly dispersed immunofluorescence (B-1, B-3, B-3). Cell dimensions and scale bars are marked in the pictures.
From: Zalecki M, Juranek J, Pidsudko Z, Mogielnicka-Brzozowska M, Kaleczyc J, Franke-Radowiecka A.
Inferior vagal ganglion galaninergic response to gastric ulcers.
PLoS One. 2020 Nov 23;15(11):e0242746.
doi: 10.1371/journal.pone.0242746.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used to identify PGP9.5 exprressing perikarya in rat nodose ganglion sections by immunofluorescence.
Image caption:
Characteristics (shapes, immunofluorescence) of PGP9.5/Gal-immunofluorescent vagal nodose perikarya occasionally observed in the control and experimental animals.
Microphotographs showing high magnifications of occasionally observed Gal-immunoreactive neurons (arrows) with large (A-1, A-3, A-3, C-1, C-2, C-3) and small (B-1, B-3, B-3, D-1, D-2, D-3) cell body sizes occurring in the vagal nodose ganglia in the control (A-1, A-3, A-3, B-1, B-3, B-3) and experimental (C-1, C-2, C-3, D-1, D-2, D-3) animals. A characteristic, clump-like Gal-immunofluorescence pattern is visible in some of the perikarya (A-1, A-3, A-3, C-1, C-2, C-3, D-1, D-2, D-3), while others are characterized by strong, evenly dispersed immunofluorescence (B-1, B-3, B-3). Cell dimensions and scale bars are marked in the pictures.
From: Zalecki M, Juranek J, Pidsudko Z, Mogielnicka-Brzozowska M, Kaleczyc J, Franke-Radowiecka A.
Inferior vagal ganglion galaninergic response to gastric ulcers.
PLoS One. 2020 Nov 23;15(11):e0242746.
doi: 10.1371/journal.pone.0242746.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase widely expressed in neuronal tissues representing, by weight 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway.
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES. Mouse anti Human protein gene product 9.5 antibody, clone 13C4 stains neuroendocrine cells in human adult gut (unlike Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3).
Clones 31A3 and 13C4 each recognize a different epitope towards the N-terminus of the protein.
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES. Mouse anti Human protein gene product 9.5 antibody, clone 13C4 stains neuroendocrine cells in human adult gut (unlike Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3).
Clones 31A3 and 13C4 each recognize a different epitope towards the N-terminus of the protein.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Guinea Pig Pig Beaver Donkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Native, from brain
- Approx. Protein Concentrations
- 1 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P09936
- Entrez Gene
- UCHL1
- GO Terms
- GO:0005886 plasma membrane
- GO:0004197 cysteine-type endopeptidase activity
- GO:0004221 ubiquitin thiolesterase activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0006511 ubiquitin-dependent protein catabolic process
- GO:0008242 omega peptidase activity
- GO:0016579 protein deubiquitination
- GO:0016874 ligase activity
- GO:0031694 alpha-2A adrenergic receptor binding
- GO:0043130 ubiquitin binding
- GO:0043407 negative regulation of MAP kinase activity
- Regulatory
- For research purposes only
Applications of Protein Gene Product 9.5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | |||
| Immunohistology - Paraffin 1 | 1/200 | ||
| Western Blotting | 1/100 | 1/1000 |
- 1 Antigen is stable in formalin fixed paraffin embedded sections, however we recommend fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding. Can be used without acid/ethanol fixation if the sections are subjected to microwave treatment in citrate buffer by standard methods.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Protein Gene Product 9.5 antibody
-
Wilson, P.O. et al. (1988) The immunolocalization of protein gene product 9.5 using rabbit polyclonal and mouse monoclonal antibodies.
Br. J. Exp. Pathol. 69: 91-104. -
Kotani, T. et al. (2010) Expression of PTPRO in the interneurons of adult mouse olfactory bulb.
J Comp Neurol. 518: 119-36. -
Buels, K.S. et al. (2012) Non-bronchodilating mechanisms of tiotropium prevent airway hyperreactivity in a guinea-pig model of allergic asthma.
Br J Pharmacol. 165: 1501-14. -
Sasaki, H. et al. (2001) Expression of the protein gene product 9.5, PGP9.5, is correlated with T-status in non-small cell lung cancer.
Jpn J Clin Oncol. 31: 532-5. -
Burliński, P.J. (2012) Inflammation- and axotomy-induced changes in cocaine- and amphetamine-regulated transcript peptide-like immunoreactive (CART-LI) nervous structures in the porcine descending colon.
Pol J Vet Sci. 15 (3): 517-24. -
Bulc, M. et al. (2012) Immunohistochemical characterization of the porcine nodose ganglion.
Acta Histochem. pii: S0065-1281(12)00142-0. -
Dudek, A. et al. (2012) Immunohistochemical characterization of neurons in the vestibular ganglion (Scarpa's ganglion) of the pig.
Pol J Vet Sci.15: 499-507. -
Zalecki, M. (2015) The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)-Neuronal Tracing Studies.
PLoS One. 10 (5): e0126958. -
Akazawa, N. et al. (2014) Neuroendocrine carcinoma of the esophagus: clinicopathologic study of 10 cases and verification of the diagnostic utility of mASH1, NeuroD1, and PGP9.5
Esophagus. 11 (4): 245-257. -
Cooke, H.J. et al. (1999) Activation of neuronal adenosine A1 receptors suppresses secretory reflexes in the guinea pig colon.
Am J Physiol. 276 (2 Pt 1): G451-62. -
Godlewski J & Pidsudko Z (2012) Characteristic of galaninergic components of the enteric nervous system in the cancer invasion of human large intestine.
Ann Anat. 194 (4): 368-72. -
Kaleczyc, J. et al. (2007) The distribution and chemical coding of intramural neurons supplying the porcine stomach - the study on normal pigs and on animals suffering from swine dysentery.
Anat Histol Embryol. 36 (3): 186-93. -
Komori, N. et al. (2003) Presence of beta-arrestin-1 immunoreactivity in the cutaneous nerve fibers of rat glabrous skin.
Brain Res. 988 (1-2): 121-9. -
Pidsudko, Z. et al. (2008) Distribution and chemical coding of intramural neurons in the porcine ileum during proliferative enteropathy.
J Comp Pathol. 138 (1): 23-31. -
Pidsudko, Z. (2013) Immunohistochemical characteristics and distribution of neurons in the intramural ganglia supplying the urinary bladder in the male pig.
Pol J Vet Sci. 16 (4): 629-38. -
Sienkiewicz, W. et al. (2000) Has active immunization against gonadotrophin-releasing hormone any effect on testis innervation in the pig? An immunohistochemical study.
Anat Histol Embryol. 29 (4): 247-54. -
Zalecki, M. et al. (2019) Enteric nervous system in the European beaver (Castor fiber) pylorus - an immunohistochemical study.
Pol J Vet Sci. 22 (1): 101-7. -
Choi, Y. et al. (2020) Stage-Dependent Expression of Protein Gene Product 9.5 in Donkey Testes.
Animals (Basel). 10 (11)Nov 20 [Epub ahead of print]. -
Szymanska, K. et al. (2018) The Influence of High and Low Doses of Bisphenol A (BPA) on the Enteric Nervous System of the Porcine Ileum.
Int J Mol Sci. 2018;19 (3): 917. -
Zalecki, M. et al. (2021) Cocaine and Amphetamine Regulated Transcript (CART) Expression Changes in the Stomach Wall Affected by Experimentally Induced Gastric Ulcerations.
Int J Mol Sci. 22 (14):7437. -
Zalecki, M. et al. (2020) Inferior vagal ganglion galaninergic response to gastric ulcers.
PLoS One. 15 (11): e0242746. -
Li, T.T. et al. (2020) Effects of different culture systems on the culture of prepuberal buffalo (Bubalus bubalis) spermatogonial stem cell-like cells in vitro.
J Vet Sci. 21 (1): e13.
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