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Protein Gene Product 9.5 antibody | 13C4

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Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 1 Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 2 Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 3 Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 4 Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 5 Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 6 Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 thumbnail image 7
Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 1
Rat brain lysate probed with Mouse anti Human Protein Gene Product 9.5 antibody, clone 13C4 (7863-2004) by western blotting
Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 2
Published customer image:
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used for the detection of PGP9.5 immunoreactive nerve fibers in porcine gastric mucosa by immunofluorescence.
Image caption:
Tissue sampling and cell measurements. (a) Picture presenting the interior surface of the "experimental animal" stomach, exposed by cutting along the greater curvature of the stomach. Ulcers are indicated by red arrows. (b) Microphotograph showing Haematoxylin and Eosin staining of the gastric ulcer margin. The gastric mucosa shows a defect (red ellipse with the inscription “Ulcer” and arrows) that extends into the deep muscular layers. (c) Diagram showing the method of tissue sampling. The tracer injection site is indicated by the violet arrows. The red vertical stripes symbolize the subsequent 0.5 cm thick transverse sections taken in specific intervals (section I—1.5 cm; section II—3.5cm; section III—5.5 cm; section IV—7.5cm) starting from the pyloric sphincter (section 0—blue vertical stripe). Subsequently, each of these sections (I, II, III, IV) was cut into 20 μm thick cryostat consecutive microscopic slices for future processing. The red circle symbolizes the localization of ulcer induced in experimental animals. (d, e, f) Photomicrographs showing: (d) bundles of PGP-immunoreactive fibers (arrowheads) and perikarya (arrows). Double arrow points to the perikaryon which was fast blue (FB) positive [the perimeter (l) and area (A) of the cell body are included]; (e) fast blue (FB) positive cell body (double arrow) with lines indicating its dimensions; (f) the image formed by merging both channels (green: PGP 9.5 and blue: FB).

From: Zalecki, M. (2015)
The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)—Neuronal Tracing Studies.
PLoS ONE 10(5): e0126958.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 3
Published customer image:
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 used for the detection of PGP9.5 immunoreactive nerve fibers in porcine descending colon by immunofluorescence.
Image caption:
Distribution pattern of nervous structures immunoreactive to protein gene-product 9.5 (PGP 9.5)—used as a panneronal marker and calcitonin gene-related peptide (CGRP) in the wall of porcine descending colon under physiological conditions (a), and after T2-toxin (b) and zearalenone (c) administration; I—myenteric plexus; II—outer submucous plexus; III—inner submucous plexus. CGRP-positive neurons (I,II,III) are indicated by arrows. Images I, II, and III are composites of merged images taken separately from green (PGP 9.5) and red (CGRP) fluorescent channels.

From: Makowska K, Obremski K, Zielonka L, Gonkowski S.
The Influence of Low Doses of Zearalenone and T-2 Toxin on Calcitonin Gene Related Peptide-Like Immunoreactive (CGRP-LI) Neurons in the ENS of the Porcine Descending Colon.
Toxins (Basel). 2017 Mar 10;9(3). pii: E98.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 4
Published customer image:
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used for the identification of PGP9.5 expressing cells in donkey testes by immunofluorescence.
Image caption:
Expression of PGP9.5 in Leydig cells of pre- and post-pubertal donkeys. Testicular tissue stained with IgG showed no immunolabeling (a,e). The expression of PGP9.5 was observed in the testes of pre-pubertal (b–d) and post-pubertal (f–h) donkeys. The white arrowheads indicate Leydig cells stained with PGP9.5. The yellow arrowheads indicate Leydig cells without PGP9.5 staining. The white bar = 50 μm.

From: Choi Y, Jung Y, Kim S, Kim J, Jung H, Yoon M.
Stage-Dependent Expression of Protein Gene Product 9.5 in Donkey Testes.
Animals (Basel). 2020 Nov 20;10(11):E2169.
doi: 10.3390/ani10112169.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 5
Published customer image:
Mouse anti Human protein gene product 9.5 antibody, clone 13C4 (7863-2004) used for the identification of PGP9.5 expressing cells in donkey testes by western blotting.
Image caption:
Cross-reactivity of protein gene product (PGP)9.5 in donkey testes. The PGP9.5 protein band appeared at the molecular size of approximately 27 kDa. The β-actin band was also detected at approximately 45 kDa in donkey testes. The negative control lane was treated with normal mouse IgG with the same concentration as the primary antibody and showed no bands.

From: Choi Y, Jung Y, Kim S, Kim J, Jung H, Yoon M.
Stage-Dependent Expression of Protein Gene Product 9.5 in Donkey Testes.
Animals (Basel). 2020 Nov 20;10(11):E2169.
doi: 10.3390/ani10112169.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 6
Published customer image:
Mouse anti Human gene product 9.5 antibody, clone 13C4 (7863-0204) used to demonstrate neurons in porcine submucosal plexus by immunofluorescence.
Image caption:
Inner submucous plexus in control animals (a) and after BPA treatment in low (b) and high (c) doses, immunostained for protein gene product 9.5 (PGP 9.5—used here as a pan-neuronal marker) and other neuronal factors including substance P (SP) and vasoactive intestinal polypeptide (VIP). Neurons showing co-localization of PGP 9.5 and other neuronal factors are indicated with arrows.

From: Szymanska K, Makowska K, Gonkowski S.
The Influence of High and Low Doses of Bisphenol A (BPA) on the Enteric Nervous System of the Porcine Ileum.
Int J Mol Sci. 2018 Mar 20;19(3):917.
doi: 10.3390/ijms19030917.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human Protein Gene Product 9.5 Antibody, clone 13C4 gallery image 7
Published customer image:
Mouse anti PGP9.5 antibody, clone 13C4 (7863-0204) used to demonstrate neurons in porcine submucosal plexus by immunofluorescence.
Image caption:
Outer submucous plexus in control animals (a) and after BPA treatment in low (b) and high (c) doses, immunostained for protein gene product 9.5 (PGP 9.5, a pan-neuronal marker) and other neuronal factors including substance P (SP) and vasoactive intestinal polypeptide (VIP). Neurons showing co-localization of PGP 9.5 and other neuronal factors are indicated with arrows.

From: Szymanska K, Makowska K, Gonkowski S.
The Influence of High and Low Doses of Bisphenol A (BPA) on the Enteric Nervous System of the Porcine Ileum.
Int J Mol Sci. 2018 Mar 20;19(3):917.
doi: 10.3390/ijms19030917.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Human Protein Gene Product 9.5

Product Type
Monoclonal Antibody
Clone
13C4
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
7863-2004
Datasheet
E IF P* WB
SDS
0.2 ml loader

Mouse anti Human protein gene product 9.5 antibody, clone 13C4 recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase widely expressed in neuronal tissues representing, by weight 1-2% of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubuiquitin pathway.

Mouse anti Human protein gene product 9.5 antibody, clone 13C4 stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumours of the DNES. Mouse anti Human protein gene product 9.5 antibody, clone 13C4 stains neuroendocrine cells in human adult gut (unlike Mouse anti Human Protein Gene Product 9.5 antibody, clone 31A3).

Clones 31A3 and 13C4 each recognize a different epitope towards the N-terminus of the protein.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Guinea Pig
Pig
Beaver
Donkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Native, from brain
Approx. Protein Concentrations
1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P09936
Entrez Gene
UCHL1
GO Terms
GO:0005886 plasma membrane
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0008242 omega peptidase activity
GO:0016579 protein deubiquitination
GO:0016874 ligase activity
GO:0031694 alpha-2A adrenergic receptor binding
GO:0043130 ubiquitin binding
GO:0043407 negative regulation of MAP kinase activity
Regulatory
For research purposes only

Applications of Protein Gene Product 9.5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin 1 1/200
Western Blotting 1/100 1/1000
  1. 1 Antigen is stable in formalin fixed paraffin embedded sections, however we recommend fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding. Can be used without acid/ethanol fixation if the sections are subjected to microwave treatment in citrate buffer by standard methods.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader

Application Based External Images

Immunofluorescence

Product Specific References

References for Protein Gene Product 9.5 antibody

  1. Wilson, P.O. et al. (1988) The immunolocalization of protein gene product 9.5 using rabbit polyclonal and mouse monoclonal antibodies.
    Br. J. Exp. Pathol. 69: 91-104.
  2. Kotani, T. et al. (2010) Expression of PTPRO in the interneurons of adult mouse olfactory bulb.
    J Comp Neurol. 518: 119-36.
  3. Buels, K.S. et al. (2012) Non-bronchodilating mechanisms of tiotropium prevent airway hyperreactivity in a guinea-pig model of allergic asthma.
    Br J Pharmacol. 165: 1501-14.
  4. Sasaki, H. et al. (2001) Expression of the protein gene product 9.5, PGP9.5, is correlated with T-status in non-small cell lung cancer.
    Jpn J Clin Oncol. 31: 532-5.
  5. Burliński, P.J. (2012) Inflammation- and axotomy-induced changes in cocaine- and amphetamine-regulated transcript peptide-like immunoreactive (CART-LI) nervous structures in the porcine descending colon.
    Pol J Vet Sci. 15 (3): 517-24.
  6. Bulc, M. et al. (2012) Immunohistochemical characterization of the porcine nodose ganglion.
    Acta Histochem. pii: S0065-1281(12)00142-0.
  7. Dudek, A. et al. (2012) Immunohistochemical characterization of neurons in the vestibular ganglion (Scarpa's ganglion) of the pig.
    Pol J Vet Sci.15: 499-507.
  8. Zalecki, M. (2015) The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)-Neuronal Tracing Studies.
    PLoS One. 10 (5): e0126958.
  9. Akazawa, N. et al. (2014) Neuroendocrine carcinoma of the esophagus: clinicopathologic study of 10 cases and verification of the diagnostic utility of mASH1, NeuroD1, and PGP9.5
    Esophagus. 11 (4): 245-257.
  10. Cooke, H.J. et al. (1999) Activation of neuronal adenosine A1 receptors suppresses secretory reflexes in the guinea pig colon.
    Am J Physiol. 276 (2 Pt 1): G451-62.
  11. Godlewski J & Pidsudko Z (2012) Characteristic of galaninergic components of the enteric nervous system in the cancer invasion of human large intestine.
    Ann Anat. 194 (4): 368-72.
  12. Kaleczyc, J. et al. (2007) The distribution and chemical coding of intramural neurons supplying the porcine stomach - the study on normal pigs and on animals suffering from swine dysentery.
    Anat Histol Embryol. 36 (3): 186-93.
  13. Komori, N. et al. (2003) Presence of beta-arrestin-1 immunoreactivity in the cutaneous nerve fibers of rat glabrous skin.
    Brain Res. 988 (1-2): 121-9.
  14. Pidsudko, Z. et al. (2008) Distribution and chemical coding of intramural neurons in the porcine ileum during proliferative enteropathy.
    J Comp Pathol. 138 (1): 23-31.
  15. Pidsudko, Z. (2013) Immunohistochemical characteristics and distribution of neurons in the intramural ganglia supplying the urinary bladder in the male pig.
    Pol J Vet Sci. 16 (4): 629-38.
  16. Sienkiewicz, W. et al. (2000) Has active immunization against gonadotrophin-releasing hormone any effect on testis innervation in the pig? An immunohistochemical study.
    Anat Histol Embryol. 29 (4): 247-54.
  17. Zalecki, M. et al. (2019) Enteric nervous system in the European beaver (Castor fiber) pylorus - an immunohistochemical study.
    Pol J Vet Sci. 22 (1): 101-7.
  18. Choi, Y. et al. (2020) Stage-Dependent Expression of Protein Gene Product 9.5 in Donkey Testes.
    Animals (Basel). 10 (11)Nov 20 [Epub ahead of print].
  19. Szymanska, K. et al. (2018) The Influence of High and Low Doses of Bisphenol A (BPA) on the Enteric Nervous System of the Porcine Ileum.
    Int J Mol Sci. 2018;19 (3): 917.

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