PI-9 antibody | 7D8

Mouse anti Human PI-9

Product Type
Monoclonal Antibody
Clone
7D8
Isotype
IgG1
Specificity
PI-9

Product Code Applications Pack Size List Price Your Price Qty
MCA2540GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * P * WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human PI-9 antibody, clone 7D8 recognizes human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a ~42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells.

Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.

Clone 7D8 has been reported to work in western blotting applications. Bio-Rad recommend the use of MCA2540GA for this purpose. Clone 7D8 is suitable for use in indirect Immunofluorescence (Hirst et al. 2003).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant PI-9 produced in P. pastoris.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/10 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 2 1/50
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Tonsil

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:RPE
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:Alk. Phos.
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:FITC
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control

References for PI-9 antibody

  1. Hirst, C.E. et al. (2001) Perforin-independent expression of granzyme B and proteinase inhibitor 9 in human testis and placenta suggests a role for granzyme B-mediated proteolysis in reproduction.
    Mol Hum Reprod. 7: 1133-42.
  2. Hirst, C.E. et al. (2003) The intracellular granzyme B inhibitor, proteinase inhibitor 9, is up-regulated during accessory cell maturation and effector cell degranulation, and its overexpression enhances CTL potency.
    J Immunol. 170 (2): 805-15.
  3. Heutinck, K.M. et al. (2012) SerpinB9 expression in human renal tubular epithelial cells is induced by triggering of the viral dsRNA sensors TLR3, MDA5 and RIG-I
    Nephrol Dial Transplant. 27: 2746-54.
  4. Buzza, M.S. et al. (2001) The granzyme B inhibitor, PI-9, is present in endothelial and mesothelial cells, suggesting that it protects bystander cells during immune responses.
    Cell Immunol. 210: 21-9.
  5. Pohjanen VM et al. (2013) Decreased expression of protease inhibitor 9, a granzyme B inhibitor, in celiac disease: a potential mechanism in enterocyte destruction and villous atrophy.
    Int J Immunopathol Pharmacol. 26 (4): 897-905.

Synonyms
SERPINB9
RRID
AB_2186599
UniProt
P50453
Entrez Gene
SERPINB9
GO Terms
GO:0002020 protease binding
GO:0002448 mast cell mediated immunity
GO:0005829 cytosol
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0006916 anti-apoptosis
GO:0006955 immune response
GO:0043027 caspase inhibitor activity
GO:0071391 cellular response to estrogen stimulus
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products