PI-9 antibody | 7D8
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Mouse anti Human PI-9
- Product Type
- Monoclonal Antibody
- Clone
- 7D8
- Isotype
- IgG1
- Specificity
- PI-9
Antibody Quality and Performance Guarantee
| Mouse anti Human PI-9 antibody, clone 7D8 recognizes human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a ~42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 has been reported to work in western blotting applications. Bio-Rad recommend the use of MCA2540GA for this purpose. Clone 7D8 is suitable for use in indirect Immunofluorescence (Hirst et al. 2003). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant PI-9 produced in P. pastoris.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/10 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 2 | |
1/50 | |
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for PI-9 antibody
-
Hirst, C.E. et al. (2001) Perforin-independent expression of granzyme B and proteinase inhibitor 9 in human testis and placenta suggests a role for granzyme B-mediated proteolysis in reproduction.
Mol Hum Reprod. 7: 1133-42. -
Hirst, C.E. et al. (2003) The intracellular granzyme B inhibitor, proteinase inhibitor 9, is up-regulated during accessory cell maturation and effector cell degranulation, and its overexpression enhances CTL potency.
J Immunol. 170 (2): 805-15. -
Heutinck, K.M. et al. (2012) SerpinB9 expression in human renal tubular epithelial cells is induced by triggering of the viral dsRNA sensors TLR3, MDA5 and RIG-I
Nephrol Dial Transplant. 27: 2746-54. -
Buzza, M.S. et al. (2001) The granzyme B inhibitor, PI-9, is present in endothelial and mesothelial cells, suggesting that it protects bystander cells during immune responses.
Cell Immunol. 210: 21-9. -
Pohjanen VM et al. (2013) Decreased expression of protease inhibitor 9, a granzyme B inhibitor, in celiac disease: a potential mechanism in enterocyte destruction and villous atrophy.
Int J Immunopathol Pharmacol. 26 (4): 897-905.
- Synonyms
- SERPINB9
- RRID
- AB_2186599
- UniProt
- P50453
- Entrez Gene
- SERPINB9
- GO Terms
- GO:0002020 protease binding
- GO:0002448 mast cell mediated immunity
- GO:0005829 cytosol
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0006916 anti-apoptosis
- GO:0006955 immune response
- GO:0043027 caspase inhibitor activity
- View More GO Terms
- GO:0071391 cellular response to estrogen stimulus
MCA2540GA
155602
163613
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