p53 antibody | DO-11

Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of p53 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Frozen | |||
Immunohistology - Paraffin 1 | 1/1000 | ||
Immunoprecipitation | |||
Western Blotting | 1/1000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Colon or breast carcinoma
Secondary Antibodies Available
Product Specific References
References for p53 antibody
-
Vojtěšek, B. et al. (1995) Conformational changes in p53 analysed using new antibodies to the core DNA binding domain of the protein.
Oncogene. 10: 389-393. -
Palecek, E. et al. (2001) Binding of p53 and its core domain to supercoiled DNA.
Eur J Biochem. 268: 573-81. -
Coomber, D.W. and Ward, .R.L. (2001) Isolation of human antibodies against the central DNA binding domain of p53 from an individual with colorectal cancer using antibody phage display.
Clin Cancer Res. 7: 2802-8. -
Wei, J. et al. (2012) Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses.
Proc Natl Acad Sci U S A. 109: E2543-50. -
Warnock, L.J. et al. (2011) Aurora A mediates cross-talk between N- and C-terminal post-translational modifications of p53.
Cancer Biol Ther. 12: 1059-68.
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