Myeloperoxidase antibody | 2C7


Mouse anti Human Myeloperoxidase:Alexa Fluor® 647
Mouse anti Human Myeloperoxidase:FITC
Mouse anti Human Myeloperoxidase
Mouse anti Human Myeloperoxidase:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 2C7
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1757A647 | F* | 100 Tests/1ml |
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MCA1757F | F* | 0.1 mg |
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MCA1757T | C E F* P | 20 µg |
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MCA1757 | C E F* P WB* | 0.2 mg |
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MCA1757PE | F* | 100 Tests |
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Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.
Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Human myeloperoxidase
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05164
- Entrez Gene
- MPO
- GO Terms
- GO:0003682 chromatin binding
- GO:0006952 defense response
- GO:0005764 lysosome
- GO:0004601 peroxidase activity
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0030141 stored secretory granule
- GO:0055114 oxidation-reduction process
- GO:0006916 anti-apoptosis
- GO:0008201 heparin binding
- GO:0020037 heme binding
- GO:0034374 low-density lipoprotein particle remodeling
- GO:0042744 hydrogen peroxide catabolic process
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of Myeloperoxidase antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry 1 | Neat | ||
Flow Cytometry 1 | Neat | 1/10 | |
ELISA | |||
Flow Cytometry 1 | 1/50 | 1/100 | |
Immunohistology - Frozen | 1/1000 | 1/5000 | |
Immunohistology - Paraffin | 1/500 | 1/1000 | |
Western Blotting 2 | |||
Flow Cytometry 1 | Neat |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 22C7 recognises myeloperoxidase under non-reducing conditions, see Audrian et al. for details
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Bone marrow
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
Source Reference
-
Audrain, M.A. et al. (1997) Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies.
Clin Exp Immunol. 107 (1): 127-34.
References for Myeloperoxidase antibody
-
Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71. -
Patry, Y.C. et al. (2003) Difference in antigenic determinant profiles between human and rat myeloperoxidase.
Clin Exp Immunol. 132 (3): 505-8. -
Zhang, N. et al. (2008) Different types of T-effector cells orchestrate mucosal inflammation in chronic sinus disease.
J Allergy Clin Immunol. 122: 961-8. -
Sloane, A.J. et al. (2005) Proteomic analysis of sputum from adults and children with cystic fibrosis and from control subjects.
Am J Respir Crit Care Med. 172: 1416-26. -
Luo, B. et al. (2013) Immunopathology features of chronic rhinosinusitis in high-altitude dwelling Tibetans.
Allergy Rhinol (Providence). 4: e69-76. -
Behnen, M. et al. (2014) Immobilized immune complexes induce neutrophil extracellular trap release by human neutrophil granulocytes via FcγRIIIB and Mac-1.
J Immunol. 193 (4): 1954-65. -
Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71. -
Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
Vet Immunol Immunopathol. 159 (1-2): 91-6. -
Wang, H. et al. (2016) Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
PLoS One. 11 (2): e0148197. -
Rai, A.K. et al. (2017) Exonal switch down-regulates the expression of CD5 on blasts of acute T cell leukaemia.
Clin Exp Immunol. 190 (3): 340-350. -
Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
Vet Comp Oncol. 14 (4): 409-16. -
Helseth, R. et al. (2019) Glucose associated NETosis in patients with ST-elevation myocardial infarction: an observational study.
BMC Cardiovasc Disord. 19 (1): 221.
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