Myeloperoxidase antibody | 2C7
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F P ResetMouse anti Human Myeloperoxidase
- Product Type
- Monoclonal Antibody
- Clone
- 2C7
- Isotype
- IgG1
- Specificity
- Myeloperoxidase
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| Mouse anti Human myeloperoxidase antibody, clone 2C7 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond. Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA. Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human myeloperoxidase
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry 1 | |
1/50 | 1/100 |
| Immunohistology - Frozen | |
1/1000 | 1/5000 |
| Immunohistology - Paraffin | |
1/500 | 1/1000 |
| Western Blotting 2 | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2Mouse anti Human myeloperoxidase antibody, clone 2C7 recognises myeloperoxidase under non-reducing conditions (Audrian et al. 1997).
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Bone marrow
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Audrain, M.A. et al. (1997) Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies.
Clin Exp Immunol. 107 (1): 127-34.
References for Myeloperoxidase antibody
-
Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71. -
Patry, Y.C. et al. (2003) Difference in antigenic determinant profiles between human and rat myeloperoxidase.
Clin Exp Immunol. 132 (3): 505-8. -
Zhang, N. et al. (2008) Different types of T-effector cells orchestrate mucosal inflammation in chronic sinus disease.
J Allergy Clin Immunol. 122: 961-8. -
Sloane, A.J. et al. (2005) Proteomic analysis of sputum from adults and children with cystic fibrosis and from control subjects.
Am J Respir Crit Care Med. 172: 1416-26. -
Luo, B. et al. (2013) Immunopathology features of chronic rhinosinusitis in high-altitude dwelling Tibetans.
Allergy Rhinol (Providence). 4: e69-76. -
Behnen, M. et al. (2014) Immobilized immune complexes induce neutrophil extracellular trap release by human neutrophil granulocytes via FcγRIIIB and Mac-1.
J Immunol. 193 (4): 1954-65. -
Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71. -
Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
Vet Immunol Immunopathol. 159 (1-2): 91-6.
View The Latest Product References
-
Wang, H. et al. (2016) Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
PLoS One. 11 (2): e0148197. -
Rai, A.K. et al. (2017) Exonal switch down-regulates the expression of CD5 on blasts of acute T cell leukaemia.
Clin Exp Immunol. 190 (3): 340-350. -
Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
Vet Comp Oncol. 14 (4): 409-16. -
Helseth, R. et al. (2019) Glucose associated NETosis in patients with ST-elevation myocardial infarction: an observational study.
BMC Cardiovasc Disord. 19 (1): 221. -
Hoppenbrouwers, T. et al. (2018) Neutrophil Extracellular Traps in Children With Meningococcal Sepsis.
Pediatr Crit Care Med. 19 (6): e286-e291. -
Manfredi, A.A. et al. (2021) Platelet phagocytosis via PSGL1 and accumulation of microparticles in systemic sclerosis.
Arthritis Rheumatol. Jul 19 [Epub ahead of print]. -
de Moraes Mazetto, B. et al. (2022) Association between neutrophil extracellular traps (NETs) and thrombosis in antiphospholipid syndrome
Thrombosis Res. 214: 132-7.
- RRID
- AB_2146467
- UniProt
- P05164
- Entrez Gene
- MPO
- GO Terms
- GO:0003682 chromatin binding
- GO:0006952 defense response
- GO:0005764 lysosome
- GO:0004601 peroxidase activity
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0030141 stored secretory granule
- GO:0055114 oxidation-reduction process
- GO:0006916 anti-apoptosis
- View More GO Terms
- GO:0008201 heparin binding
- GO:0020037 heme binding
- GO:0034374 low-density lipoprotein particle remodeling
- GO:0042744 hydrogen peroxide catabolic process
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