Myeloperoxidase antibody | 2C7

Mouse anti Human Myeloperoxidase

Product Type
Monoclonal Antibody
Clone
2C7
Isotype
IgG1
Specificity
Myeloperoxidase

Product Code Applications Pack Size List Price Your Price Qty
MCA1757
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F * P WB * 0.2 mg loader
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loader
MCA1757T
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SDS Safety Datasheet SDS
C E F * P 20 µg loader
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Mouse anti Human myeloperoxidase antibody, clone 2C7 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond.

Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.

Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human myeloperoxidase
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/50 1/100
Immunohistology - Frozen 1/1000 1/5000
Immunohistology - Paraffin 1/500 1/1000
Western Blotting 2
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2Mouse anti Human myeloperoxidase antibody, clone 2C7 recognises myeloperoxidase under non-reducing conditions (Audrian et al. 1997).
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Bone marrow

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Source Reference

  1. Audrain, M.A. et al. (1997) Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies.
    Clin Exp Immunol. 107 (1): 127-34.

References for Myeloperoxidase antibody

  1. Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
    Vet Clin Pathol. 35 (1): 55-71.
  2. Patry, Y.C. et al. (2003) Difference in antigenic determinant profiles between human and rat myeloperoxidase.
    Clin Exp Immunol. 132 (3): 505-8.
  3. Zhang, N. et al. (2008) Different types of T-effector cells orchestrate mucosal inflammation in chronic sinus disease.
    J Allergy Clin Immunol. 122: 961-8.
  4. Sloane, A.J. et al. (2005) Proteomic analysis of sputum from adults and children with cystic fibrosis and from control subjects.
    Am J Respir Crit Care Med. 172: 1416-26.
  5. Luo, B. et al. (2013) Immunopathology features of chronic rhinosinusitis in high-altitude dwelling Tibetans.
    Allergy Rhinol (Providence). 4: e69-76.
  6. Behnen, M. et al. (2014) Immobilized immune complexes induce neutrophil extracellular trap release by human neutrophil granulocytes via FcγRIIIB and Mac-1.
    J Immunol. 193 (4): 1954-65.
  7. Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
    Vet Clin Pathol. 35 (1): 55-71.
  8. Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
    Vet Immunol Immunopathol. 159 (1-2): 91-6.
  9. View The Latest Product References
  10. Wang, H. et al. (2016) Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
    PLoS One. 11 (2): e0148197.
  11. Rai, A.K. et al. (2017) Exonal switch down-regulates the expression of CD5 on blasts of acute T cell leukaemia.
    Clin Exp Immunol. 190 (3): 340-350.
  12. Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
    Vet Comp Oncol. 14 (4): 409-16.
  13. Helseth, R. et al. (2019) Glucose associated NETosis in patients with ST-elevation myocardial infarction: an observational study.
    BMC Cardiovasc Disord. 19 (1): 221.
  14. Hoppenbrouwers, T. et al. (2018) Neutrophil Extracellular Traps in Children With Meningococcal Sepsis.
    Pediatr Crit Care Med. 19 (6): e286-e291.
  15. Manfredi, A.A. et al. (2021) Platelet phagocytosis via PSGL1 and accumulation of microparticles in systemic sclerosis.
    Arthritis Rheumatol. Jul 19 [Epub ahead of print].
  16. de Moraes Mazetto, B. et al. (2022) Association between neutrophil extracellular traps (NETs) and thrombosis in antiphospholipid syndrome
    Thrombosis Res. 214: 132-7.

RRID
AB_2146467
UniProt
P05164
Entrez Gene
MPO
GO Terms
GO:0003682 chromatin binding
GO:0006952 defense response
GO:0005764 lysosome
GO:0004601 peroxidase activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0030141 stored secretory granule
GO:0055114 oxidation-reduction process
GO:0006916 anti-apoptosis
GO:0008201 heparin binding
GO:0020037 heme binding
GO:0034374 low-density lipoprotein particle remodeling
GO:0042744 hydrogen peroxide catabolic process
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