Myeloperoxidase antibody | 2C7

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Anti Human Myeloperoxidase Antibody, clone 2C7 thumbnail image 1

Anti Human Myeloperoxidase Antibody, clone 2C7 thumbnail image 2

Anti Human Myeloperoxidase Antibody, clone 2C7 gallery image 1 — Staining of human bone marrow. Formalin fixed, paraffin processed tissue with Mouse anti Human Myeloperoxidase (**MCA1757T**).

Anti Human Myeloperoxidase Antibody, clone 2C7 gallery image 2 — Figure A. FITC conjugated Mouse anti Human CD66b (MCA216F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Human CD66b (MCA216F) and Alexa Fluor 647 conjugated Mouse anti Human Myeloperoxidase (MCA1757A647). All experiments performed on Leucoperm (BUF09) fixed and permeabilised human peripheral blood gated on single cell granulocytes. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human Myeloperoxidase:Alexa Fluor® 647

Mouse anti Human Myeloperoxidase:FITC

Mouse anti Human Myeloperoxidase

Mouse anti Human Myeloperoxidase:RPE

Product Type: Monoclonal Antibody
Clone: 2C7
Isotype: IgG1

Product Code	Applications	Pack Size
MCA1757A647	F*	100 Tests/1ml
MCA1757F	F*	0.1 mg
MCA1757T	C E F* P	20 µg
MCA1757	C E F* P WB*	0.2 mg
MCA1757PE	F*	100 Tests

Mouse anti Human myeloperoxidase antibody, clone 2C7 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond.

Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.

Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Dog

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alexa Fluor 647 - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Immunogen

Human myeloperoxidase

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

UniProt: P05164 Related reagents
Entrez Gene: MPO Related reagents
GO Terms: GO:0003682 chromatin binding; GO:0006952 defense response; GO:0005764 lysosome; GO:0004601 peroxidase activity; GO:0005615 extracellular space; GO:0005634 nucleus; GO:0030141 stored secretory granule; GO:0055114 oxidation-reduction process; GO:0006916 anti-apoptosis; GO:0008201 heparin binding; GO:0020037 heme binding; GO:0034374 low-density lipoprotein particle remodeling; GO:0042744 hydrogen peroxide catabolic process
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of Myeloperoxidase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat
Flow Cytometry ¹	Neat	1/10
ELISA
Flow Cytometry ¹	1/50	1/100
Immunohistology - Frozen	1/1000	1/5000
Immunohistology - Paraffin	1/500	1/1000
Western Blotting ²
Flow Cytometry ¹		Neat

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (Product Code BUF09) for this purpose.

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (Product Code BUF09) for this purpose.

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (Product Code BUF09) for this purpose.
²2C7 recognises myeloperoxidase under non-reducing conditions, see Audrian et al. for details

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm^™ (Product Code BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested wokring dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Histology Positive Control Tissue: Bone marrow

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Product Specific References

References for Myeloperoxidase antibody

Audrain, M.A. et al. (1997) Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies.
Clin Exp Immunol. 107 (1): 127-34.
Patry, Y.C. et al. (2003) Difference in antigenic determinant profiles between human and rat myeloperoxidase.
Clin Exp Immunol. 132 (3): 505-8.
Zhang, N. et al. (2008) Different types of T-effector cells orchestrate mucosal inflammation in chronic sinus disease.
J Allergy Clin Immunol. 122: 961-8.
Sloane, A.J. et al. (2005) Proteomic analysis of sputum from adults and children with cystic fibrosis and from control subjects.
Am J Respir Crit Care Med. 172: 1416-26.
Luo, B. et al. (2013) Immunopathology features of chronic rhinosinusitis in high-altitude dwelling Tibetans.
Allergy Rhinol (Providence). 4: e69-76.
Behnen, M. et al. (2014) Immobilized immune complexes induce neutrophil extracellular trap release by human neutrophil granulocytes via FcγRIIIB and Mac-1.
J Immunol. 193 (4): 1954-65.
Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71.
Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
Vet Immunol Immunopathol. 159 (1-2): 91-6.
Wang, H. et al. (2016) Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
PLoS One. 11 (2): e0148197.
Rai, A.K. et al. (2017) Exonal switch down-regulates the expression of CD5 on blasts of acute T cell leukaemia.
Clin Exp Immunol. 190 (3): 340-350.
Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
Vet Comp Oncol. 14 (4): 409-16.
Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
Vet Clin Pathol. 35 (1): 55-71.

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