MCP-1 antibody | 5D3-F7

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Mouse anti Human MCP-1

Product Type
Monoclonal Antibody
Clone
5D3-F7
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5981GA C E F* IP P* WB 0.1 mg
Mouse anti Human MCP-1, clone 5D3-F7 recognizes Monocyte chemoattractant protein-1, otherwise known as CCL2 (C-C motif chemokine 2), a member of the intercrine beta (chemokine CC) family, which acts as a chemoattractant for monocytes, basophils, natural killer cells (NKs) and memory T cells, in both normal and neoplastic tissues (Peri et al. 1994).

MCP-1 can be synthesized on demand in response to inflammatory stimuli, and is expressed in a wide variety of tissues and by many cell types, including macrophages, basophils, NK cells, T cells, immature DCs, endothelial cells. astrocytes and microglia. MCP-1 acts as a major ligand for CCR2 (C-C chemokine receptor type 2), and is implicated in several inflammatory diseases, including Rheumatoid Arthritis (RA), Multiple Sclerosis (MS), asthma and atherosclerosis (Bendall, 2005.)

MCP-1 was originally cloned from human gliomas and myelomoncytic cells, and overexpression of MCP-1 has long been associated with cancers, including breast, ovarian, prostate and esophageal cancer and also more recently with an increase in metastatic potential, for example in bone and lung cancer (Zhang et al. 2010). Clone 5D3-F7 has been shown to recognize both recombinant and natural human MCP-1, and to inhibit the chemotactic activity of MCP-1 for monocytes (Peri et al. 1994). Bio-Rad recommend the removal of sodium azide for this purpose.

Mouse anti Human MCP-1, clone 5D3-F7 does not cross react with closely related MCP-2, MCP-3, MIP-1alpha, and RANTES, or with the CXC chemokines NAP-2, gro-alpha and interleukin-8.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human MCP-1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P13500 Related reagents
Entrez Gene
CCL2 Related reagents
GO Terms
GO:0002548 monocyte chemotaxis
GO:0007155 cell adhesion
GO:0004672 protein kinase activity
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006468 protein phosphorylation
GO:0006874 cellular calcium ion homeostasis
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006959 humoral immune response
GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
GO:0007259 JAK-STAT cascade
GO:0008009 chemokine activity
GO:0019079 viral genome replication
GO:0031727 CCR2 chemokine receptor binding
GO:0048246 macrophage chemotaxis
Regulatory
For research purposes only

Applications of MCP-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 4.0ug/ml
Flow Cytometry 1 Neat 1/5
Immunohistology - Frozen
Immunohistology - Paraffin 2
Immunoprecipitation
Western Blotting 1/100 1/1000
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  2. 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil, lymph node or spleen

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Product Specific References

References for MCP-1 antibody

  1. Peri, G. et al. (1994) A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells.
    J Immunol Methods. 174 (1-2): 249-57.

Further Reading

  1. Bendall, L. (2005) Chemokines and their receptors in disease.
    Histol Histopathol. 20 (3): 907-26.
  2. Zhang, J. et al. (2010) Targeting chemokine (C-C motif) ligand 2 (CCL2) as an example of translation of cancer molecular biology to the clinic.
    Prog Mol Biol Transl Sci. 95: 31–53