MCP-1 antibody | 5D3-F7

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Mouse anti Human MCP-1

Product Type: Monoclonal Antibody
Clone: 5D3-F7
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA5981GA	C E F* IP P* WB			0.1 mg

Mouse anti Human MCP-1, clone 5D3-F7 recognizes Monocyte chemoattractant protein-1, otherwise known as CCL2 (C-C motif chemokine 2), a member of the intercrine beta (chemokine CC) family, which acts as a chemoattractant for monocytes, basophils, natural killer cells (NKs) and memory T cells, in both normal and neoplastic tissues (Peri et al. 1994).

MCP-1 can be synthesized on demand in response to inflammatory stimuli, and is expressed in a wide variety of tissues and by many cell types, including macrophages, basophils, NK cells, T cells, immature DCs, endothelial cells. astrocytes and microglia. MCP-1 acts as a major ligand for CCR2 (C-C chemokine receptor type 2), and is implicated in several inflammatory diseases, including Rheumatoid Arthritis (RA), Multiple Sclerosis (MS), asthma and atherosclerosis (Bendall, 2005.)

MCP-1 was originally cloned from human gliomas and myelomoncytic cells, and overexpression of MCP-1 has long been associated with cancers, including breast, ovarian, prostate and esophageal cancer and also more recently with an increase in metastatic potential, for example in bone and lung cancer (Zhang et al. 2010). Clone 5D3-F7 has been shown to recognize both recombinant and natural human MCP-1, and to inhibit the chemotactic activity of MCP-1 for monocytes (Peri et al. 1994). Bio-Rad recommend the removal of sodium azide for this purpose.

Mouse anti Human MCP-1, clone 5D3-F7 does not cross react with closely related MCP-2, MCP-3, MIP-1alpha, and RANTES, or with the CXC chemokines NAP-2, gro-alpha and interleukin-8.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Cynomolgus monkey
Rhesus Monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

Recombinant human MCP-1

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P13500 Related reagents
Entrez Gene: CCL2 Related reagents
GO Terms: GO:0002548 monocyte chemotaxis; GO:0007155 cell adhesion; GO:0004672 protein kinase activity; GO:0004871 signal transducer activity; GO:0005615 extracellular space; GO:0006468 protein phosphorylation; GO:0006874 cellular calcium ion homeostasis; GO:0006916 anti-apoptosis; GO:0006954 inflammatory response; GO:0006959 humoral immune response; GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger; GO:0007259 JAK-STAT cascade; GO:0008009 chemokine activity; GO:0019079 viral genome replication; GO:0031727 CCR2 chemokine receptor binding; GO:0048246 macrophage chemotaxis
Regulatory: For research purposes only

Applications of MCP-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1.0	4.0ug/ml
Flow Cytometry ¹	Neat	1/5
Immunohistology - Frozen
Immunohistology - Paraffin ²
Immunoprecipitation
Western Blotting	1/100	1/1000

¹ Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
² This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Histology Positive Control Tissue: Human tonsil, lymph node or spleen

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Product Specific References

References for MCP-1 antibody

Peri, G. et al. (1994) A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells.
J Immunol Methods. 174 (1-2): 249-57.