MCP-1 antibody | 5D3-F7
Mouse anti Human MCP-1
- Product Type
- Monoclonal Antibody
|Mouse anti Human MCP-1, clone 5D3-F7 recognizes Monocyte chemoattractant protein-1, otherwise known as CCL2 (C-C motif chemokine 2), a member of the intercrine beta (chemokine CC) family, which acts as a chemoattractant for monocytes, basophils, natural killer cells (NKs) and memory T cells, in both normal and neoplastic tissues (Peri et al. 1994).
MCP-1 can be synthesized on demand in response to inflammatory stimuli, and is expressed in a wide variety of tissues and by many cell types, including macrophages, basophils, NK cells, T cells, immature DCs, endothelial cells. astrocytes and microglia. MCP-1 acts as a major ligand for CCR2 (C-C chemokine receptor type 2), and is implicated in several inflammatory diseases, including Rheumatoid Arthritis (RA), Multiple Sclerosis (MS), asthma and atherosclerosis (Bendall, 2005.)
MCP-1 was originally cloned from human gliomas and myelomoncytic cells, and overexpression of MCP-1 has long been associated with cancers, including breast, ovarian, prostate and esophageal cancer and also more recently with an increase in metastatic potential, for example in bone and lung cancer (Zhang et al. 2010). Clone 5D3-F7 has been shown to recognize both recombinant and natural human MCP-1, and to inhibit the chemotactic activity of MCP-1 for monocytes (Peri et al. 1994). Bio-Rad recommend the removal of sodium azide for this purpose.
Mouse anti Human MCP-1, clone 5D3-F7 does not cross react with closely related MCP-2, MCP-3, MIP-1alpha, and RANTES, or with the CXC chemokines NAP-2, gro-alpha and interleukin-8.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Recombinant human MCP-1.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||Neat||1/5|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 2|
- 1 Results maybe enhanced using membrane permeabilisation. Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Human tonsil, lymph node or spleen
References for MCP-1 antibody
Hirsch, A.J. & Shenk, T. (1999) Human cytomegalovirus inhibits transcription of the CC chemokine MCP-1 gene.
J Virol. 73 (1): 404-10.
Corrigall, V.M. et al. (2001) Functional IL-2 receptor beta (CD122) and gamma (CD132) chains are expressed by fibroblast-like synoviocytes: activation by IL-2 stimulates monocyte chemoattractant protein-1 production.
J Immunol. 166 (6): 4141-7.
Rezaie-majd, A. et al. (2002) Simvastatin reduces expression of cytokines interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 in circulating monocytes from hypercholesterolemic patients.
Arterioscler Thromb Vasc Biol. 22 (7): 1194-9.
Karrer, S. et al. (2005) The -2518 promotor polymorphism in the MCP-1 gene is associated with systemic sclerosis.
J Invest Dermatol. 124 (1): 92-8.
Peri, G. et al. (1994) A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells.
J Immunol Methods. 174 (1-2): 249-57.
- Entrez Gene
- GO Terms
- GO:0002548 monocyte chemotaxis
- GO:0007155 cell adhesion
- GO:0004672 protein kinase activity
- GO:0004871 signal transducer activity
- GO:0005615 extracellular space
- GO:0006468 protein phosphorylation
- GO:0006874 cellular calcium ion homeostasis
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- View More GO Terms
- GO:0006959 humoral immune response
- GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
- GO:0007259 JAK-STAT cascade
- GO:0008009 chemokine activity
- GO:0019079 viral genome replication
- GO:0031727 CCR2 chemokine receptor binding
- GO:0048246 macrophage chemotaxis
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