MCP-1 antibody | 5D3-F7
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Mouse anti Human MCP-1, clone 5D3-F7 recognizes Monocyte chemoattractant protein-1, otherwise known as CCL2 (C-C motif chemokine 2), a member of the intercrine beta (chemokine CC) family, which acts as a chemoattractant for monocytes, basophils, natural killer cells (NKs) and memory T cells, in both normal and neoplastic tissues (Peri et al. 1994).
MCP-1 can be synthesized on demand in response to inflammatory stimuli, and is expressed in a wide variety of tissues and by many cell types, including macrophages, basophils, NK cells, T cells, immature DCs, endothelial cells. astrocytes and microglia. MCP-1 acts as a major ligand for CCR2 (C-C chemokine receptor type 2), and is implicated in several inflammatory diseases, including Rheumatoid Arthritis (RA), Multiple Sclerosis (MS), asthma and atherosclerosis (Bendall, 2005.)
MCP-1 was originally cloned from human gliomas and myelomoncytic cells, and overexpression of MCP-1 has long been associated with cancers, including breast, ovarian, prostate and esophageal cancer and also more recently with an increase in metastatic potential, for example in bone and lung cancer (Zhang et al. 2010). Clone 5D3-F7 has been shown to recognize both recombinant and natural human MCP-1, and to inhibit the chemotactic activity of MCP-1 for monocytes (Peri et al. 1994). Bio-Rad recommend the removal of sodium azide for this purpose.
Mouse anti Human MCP-1, clone 5D3-F7 does not cross react with closely related MCP-2, MCP-3, MIP-1alpha, and RANTES, or with the CXC chemokines NAP-2, gro-alpha and interleukin-8.
MCP-1 can be synthesized on demand in response to inflammatory stimuli, and is expressed in a wide variety of tissues and by many cell types, including macrophages, basophils, NK cells, T cells, immature DCs, endothelial cells. astrocytes and microglia. MCP-1 acts as a major ligand for CCR2 (C-C chemokine receptor type 2), and is implicated in several inflammatory diseases, including Rheumatoid Arthritis (RA), Multiple Sclerosis (MS), asthma and atherosclerosis (Bendall, 2005.)
MCP-1 was originally cloned from human gliomas and myelomoncytic cells, and overexpression of MCP-1 has long been associated with cancers, including breast, ovarian, prostate and esophageal cancer and also more recently with an increase in metastatic potential, for example in bone and lung cancer (Zhang et al. 2010). Clone 5D3-F7 has been shown to recognize both recombinant and natural human MCP-1, and to inhibit the chemotactic activity of MCP-1 for monocytes (Peri et al. 1994). Bio-Rad recommend the removal of sodium azide for this purpose.
Mouse anti Human MCP-1, clone 5D3-F7 does not cross react with closely related MCP-2, MCP-3, MIP-1alpha, and RANTES, or with the CXC chemokines NAP-2, gro-alpha and interleukin-8.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human MCP-1
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P13500 Related reagents
- Entrez Gene
- CCL2 Related reagents
- GO Terms
- GO:0002548 monocyte chemotaxis
- GO:0007155 cell adhesion
- GO:0004672 protein kinase activity
- GO:0004871 signal transducer activity
- GO:0005615 extracellular space
- GO:0006468 protein phosphorylation
- GO:0006874 cellular calcium ion homeostasis
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0006959 humoral immune response
- GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
- GO:0007259 JAK-STAT cascade
- GO:0008009 chemokine activity
- GO:0019079 viral genome replication
- GO:0031727 CCR2 chemokine receptor binding
- GO:0048246 macrophage chemotaxis
- Regulatory
- For research purposes only
Applications of MCP-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1.0 | 4.0ug/ml | |
| Flow Cytometry 1 | Neat | 1/5 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 2 | |||
| Immunoprecipitation | |||
| Western Blotting | 1/100 | 1/1000 |
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
- 2 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Human tonsil, lymph node or spleen
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for MCP-1 antibody
-
Peri, G. et al. (1994) A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells.
J Immunol Methods. 174 (1-2): 249-57.
Further Reading
-
Bendall, L. (2005) Chemokines and their receptors in disease.
Histol Histopathol. 20 (3): 907-26. -
Zhang, J. et al. (2010) Targeting chemokine (C-C motif) ligand 2 (CCL2) as an example of translation of cancer molecular biology to the clinic.
Prog Mol Biol Transl Sci. 95: 31–53
