Mouse anti MBP antibody, clone 26
recognizes an epitope between amino acids 67 and 74 of human myelin basic protein, also known as MBP, Myelin A1 protein or Myelin membrane encephalitogenic protein. MBP is a 304 amino acid ~33 kDa abundant component of the myelin membrane. There are 6 potential isoforms of MBP generated by alternative splicing, 4 of which lack the N-terminal region containing the immunogen sequence. Mouse anti MBP antibody, clone 26 is expected to recognize isoforms 1 and 2 (UniProt: P02686
) . The N-terminal region of MBP is highly conserved and Mouse anti MBP antibody, clone 26 is expected to be broadly species cross reactive for isoforms maintaining the N-terminal region.
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Tissue Culture Supernatant containing 0.1M Tris/HCl and foetal calf serum.
- Preservative Stabilisers
- Human myelin basic protein.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MBP antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for MBP antibody
Groome, N. et al. (1988) New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein.
J Neuroimmunol. 19 (4): 305-15.
Martenson, R.E. (1984) Experimental allergic encephalomyelitis. A useful model of multiple sclerosis
Eds Alvord, Kies and Suckling pp 511-521. Alan Liss N.Y.
Sanz-Rodriguez, M. et al. (2018) R-Ras1 and R-Ras2 Are Essential for Oligodendrocyte Differentiation and Survival for Correct Myelination in the Central Nervous System.
J Neurosci. 38 (22): 5096-110.
Alcover-Sanchez, B. et al. (2020) Absence of R-Ras1 and R-Ras2 causes mitochondrial alterations that trigger axonal degeneration in a hypomyelinating disease model.
Glia. Oct 03 [Epub ahead of print].
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