IgM antibody | M15/8

Product Code Applications Pack Size List Price Your Price Qty
MCA1662
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SDS Safety Datasheet SDS
C E F IP 0.2 mg loader
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loader
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Mouse anti Human IgM monoclonal antibody, clone M15/8 recognizes human IgM, binding to the μ heavy chain. The antibody does not cross-react with other immunoglobulin classes.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml as coating Ab
Flow Cytometry 20ug/ml
Immunohistology - Frozen 20ug/ml
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

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Description Mouse IgG1 Negative Control

References for IgM antibody

  1. Cragg, M.S. et al. (1999) Analysis of the interaction of monoclonal antibodies with surface IgM on neoplastic B-cells.
    Br J Cancer. 79 (5-6): 850-7.
  2. Irie, E. et al. (2010) Severe hypogammaglobulinemia persisting for 6 years after treatment with rituximab combined chemotherapy due to arrest of B lymphocyte differentiation together with alteration of T lymphocyte homeostasis.
    Int J Hematol. 91: 501-8.
  3. Creasey, A.M. et al. (2003) Nonspecific immunoglobulin M binding and chondroitin sulfate A binding are linked phenotypes of Plasmodium falciparum isolates implicated in malaria during pregnancy.
    Infect Immun. 71: 4767-71.
  4. Ghumra, A. et al. (2012) Induction of strain-transcending antibodies against Group A PfEMP1 surface antigens from virulent malaria parasites.
    PLoS Pathog. 8 (4): e1002665.
  5. Semblat, J.P. et al. (2015) Identification of the minimal binding region of a Plasmodium falciparum IgM binding PfEMP1 domain.
    Mol Biochem Parasitol. 201 (1): 76-82.

Immunofluorescence

RRID
AB_321861
UniProt
P01871
Entrez Gene
IGHM
GO Terms
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005624 membrane fraction
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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