IgM antibody | CM7

Product Code Applications Pack Size List Price Your Price Qty
MCA1894
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SDS Safety Datasheet SDS
E F P WB 0.25 mg loader
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loader
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Mouse anti Human IgM antibody, clone CM7 is an excellent broad species anti-IgM monoclonal antibody, and shows no cross-reactivity with other immunoglobulin classes or sub classes.

Mouse anti Human IgM antibody, clone CM7 may be used as a marker of mature B lymphocytes in flow cytometry.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Pig
Marmoset
Dog
Cat
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Purified human IgM, and then purified cat IgM.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/50
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

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Description Mouse IgG1 Negative Control

References for IgM antibody

  1. Reubel, G.H. et al. (1992) Interaction of acute feline herpesvirus-1 and chronic feline immunodeficiency virus infections in experimentally infected specific pathogen free cats.
    Vet Immunol Immunopathol. 35 (1-2): 95-119.
  2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62 (2): 101-43.
  3. Chandhasin, C. et al. (2005) Unique long terminal repeat and surface glycoprotein gene sequences of feline leukemia virus as determinants of disease outcome.
    J Virol. 79 (9): 5278-87.
  4. Prieto, J.M. et al. (2017) Developmental expression of B cell molecules in equine lymphoid tissues.
    Vet Immunol Immunopathol. 183: 60-71.
  5. Tallmadge, R.L. et al. (2012) Expression of essential B cell development genes in horses with common variable immunodeficiency
    Molecular Immunology. 51 (2): 169-76.

Immunohistology - Frozen

RRID
AB_323088
UniProt
P01871
Entrez Gene
IGHM
GO Terms
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005624 membrane fraction
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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