IgM antibody | CM7
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Mouse anti Human IgM antibody, clone CM7 is an excellent broad species anti-IgM monoclonal antibody, and shows no cross-reactivity with other immunoglobulin classes or sub classes.
Mouse anti Human IgM antibody, clone CM7 may be used as a marker of mature B lymphocytes in flow cytometry.
Mouse anti Human IgM antibody, clone CM7 may be used as a marker of mature B lymphocytes in flow cytometry.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Horse Pig Marmoset Dog Cat Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Purified human IgM, and then purified cat IgM.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01871
- Entrez Gene
- IGHM
- GO Terms
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- Regulatory
- For research purposes only
Applications of IgM antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | 1/10 | 1/50 | |
| Immunohistology - Paraffin | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for IgM antibody
-
Reubel, G.H. et al. (1992) Interaction of acute feline herpesvirus-1 and chronic feline immunodeficiency virus infections in experimentally infected specific pathogen free cats.
Vet Immunol Immunopathol. 35 (1-2): 95-119. -
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43. -
Chandhasin, C. et al. (2005) Unique long terminal repeat and surface glycoprotein gene sequences of feline leukemia virus as determinants of disease outcome.
J Virol. 79 (9): 5278-87. -
Prieto, J.M. et al. (2017) Developmental expression of B cell molecules in equine lymphoid tissues.
Vet Immunol Immunopathol. 183: 60-71. -
Tallmadge, R.L. et al. (2012) Expression of essential B cell development genes in horses with common variable immunodeficiency
Molecular Immunology. 51 (2): 169-76.
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