IgM antibody | CM7

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Mouse anti Human IgM

Product Type: Monoclonal Antibody
Clone: CM7
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1894	E F P WB			0.25 mg

Mouse anti Human IgM antibody, clone CM7 is an excellent broad species anti-IgM monoclonal antibody, and shows no cross-reactivity with other immunoglobulin classes or sub classes.

Mouse anti Human IgM antibody, clone CM7 may be used as a marker of mature B lymphocytes in flow cytometry.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Horse
Pig
Marmoset
Dog
Cat
Rhesus Monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.05%

Sodium Azide

Immunogen

Purified human IgM, and then purified cat IgM.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01871 Related reagents
Entrez Gene: IGHM Related reagents
GO Terms: GO:0003823 antigen binding; GO:0016021 integral to membrane; GO:0005624 membrane fraction
Regulatory: For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Flow Cytometry	1/10	1/50
Immunohistology - Paraffin
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for IgM antibody

Reubel, G.H. et al. (1992) Interaction of acute feline herpesvirus-1 and chronic feline immunodeficiency virus infections in experimentally infected specific pathogen free cats.
Vet Immunol Immunopathol. 35 (1-2): 95-119.
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43.
Chandhasin, C. et al. (2005) Unique long terminal repeat and surface glycoprotein gene sequences of feline leukemia virus as determinants of disease outcome.
J Virol. 79 (9): 5278-87.
Prieto, J.M. et al. (2017) Developmental expression of B cell molecules in equine lymphoid tissues.
Vet Immunol Immunopathol. 183: 60-71.
Tallmadge, R.L. et al. (2012) Expression of essential B cell development genes in horses with common variable immunodeficiency
Molecular Immunology. 51 (2): 169-76.

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