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IgG4 antibody | HP6025

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Mouse anti Human IgG4:HRP

Mouse anti Human IgG4

Product Type
Monoclonal Antibody
Clone
HP6025
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2098P
Datasheet
C E P
SDS
0.2 mg loader

MCA2098G
Datasheet
C E IB P WB
SDS
0.2 mg loader

Mouse anti Human IgG4 antibody, clone HP6025 recognizes the heavy chain of human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA (Jefferis et al. 1985).

Elevated levels of IgG4 and of IgG4 presenting plasma cells are frequently seen in patients with autoimmune pancreatitis (IAP) and inflammatory bowel disease (Navaneethan et al. 2011) and it is suggested that IAP may develop as a paraneoplastic syndrome in some cancer patients (Shiokawa et al. 2013)

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified IgG4
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with SP2/0 - Ag14 mouse myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01861
Entrez Gene
IGHG4
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/20000
Immunohistology - Frozen 1/40 1/80
Immunohistology - Paraffin
ELISA
Immunoblotting
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Application Based External Images

ELISA

Immunohistology - Paraffin

Product Specific References

References for IgG4 antibody

  1. Jefferis, R. et al. (1985) Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study.
    Immunol Lett. 10 (3-4): 223-52.
  2. Agaimy, A. et al. (2010) Calcifying fibrous tumor of the stomach: clinicopathologic and molecular study of seven cases with literature review and reappraisal of histogenesis.
    Am J Surg Pathol. 34: 271-8.
  3. Strehl, J.D. et al. (2011) Numerous IgG4-positive plasma cells are ubiquitous in diverse localised non-specific chronic inflammatory conditions and need to be distinguished from IgG4-related systemic disorders.
    J Clin Pathol. 64 (3): 237-43.
  4. Black, C.M. et al. (1991) Human markers for IgG2 and IgG4 appear to be on the same molecule in the chimpanzee.
    Immunology.72: 94-8.
  5. Fernandez-Becerra, C. (2010) Naturally-acquired humoral immune responses against the N- and C-termini of the Plasmodium vivax MSP1 protein in endemic regions of Brazil and Papua New Guinea using a multiplex assay.
    Malar J. 9: 29.
  6. Lee, H.W. et al. (2008) High levels of antibodies to Plasmodium falciparum liver stage antigen-1 in naturally infected individuals in Myanmar.
    Korean J Parasitol. 46: 195-8.
  7. Shiokawa, M. et al. (2013) Risk of cancer in patients with autoimmune pancreatitis.
    Am J Gastroenterol. 108 (4): 610-7.
  8. Whelan, S.F. et al. (2013) Distinct characteristics of antibody responses against factor VIII in healthy individuals and in different cohorts of hemophilia A patients.
    Blood. 121: 1039-48.
  9. Fujimoto, M. et al. (2013) Stromal plasma cells expressing immunoglobulin G4 subclass in non-small cell lung cancer.
    Hum Pathol. 44 (8): 1569-76.
  10. Miyagawa-Hayashino, A. et al. (2009) High ratio of IgG4-positive plasma cell infiltration in cutaneous plasmacytosis--is this a cutaneous manifestation of IgG4-related disease?
    Hum Pathol. 40: 1269-77.
  11. Yamashita, K. et al. (2008) Lung involvement in IgG4-related lymphoplasmacytic vasculitis and interstitial fibrosis: report of 3 cases and review of the literature.
    Am J Surg Pathol. 32: 1620-6.
  12. Yamashita, K. et al. (2008) Degree of IgG4+ plasma cell infiltration in retroperitoneal fibrosis with or without multifocal fibrosclerosis
    Histopathology. 52: 404-9.
  13. Navaneethan, U. et al. (2011) Tissue infiltration of IgG4+ plasma cells in symptomatic patients with ileal pouch-anal anastomosis
    J Crohns Colitis. 5: 570-6.
  14. Shiokawa, M. et al. (2013) Risk of cancer in patients with autoimmune pancreatitis.
    Am J Gastroenterol. 108 (4): 610-7.
  15. Engelmann, R. et al. (2015) Bone resorption correlates with the frequency of CD5⁺ B cells in the blood of patients with rheumatoid arthritis.
    Rheumatology (Oxford). 54 (3): 545-53.
  16. Ráty, S. et al. (2015) Tumor-like Chronic Pancreatitis Is Often Autoimmune Pancreatitis.
    Anticancer Res. 35 (11): 6163-6.
  17. Agaimy, A. et al. (2015) SMARCA4-deficient undifferentiated carcinoma of the ovary (small cell carcinoma, hypercalcemic type): clinicopathologic and immunohistochemical study of 3 cases.
    Ann Diagn Pathol. 19 (5): 283-7.
  18. Riedemann, N.C. et al. (2017) Controlling the anaphylatoxin C5a in diseases requires a specifically targeted inhibition.
    Clin Immunol. 180: 25-32.
  19. Pan, Q. et al. (2016) Association between IgG4 Autoantibody and Complement Abnormalities in Systemic Lupus Erythematosus.
    Mediators Inflamm. 2016: 2196986.
  20. Engelmann, R. et al. (2018) Decreased IgG4 ACPA levels in responders and increased CD1c+ classical dendritic cells in non-responders of patients with rheumatoid arthritis under therapy.
    Clin Rheumatol. 37 (7): 1783-90.

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