IgG4 antibody | HP6025
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Mouse anti Human IgG4 antibody, clone HP6025 recognizes the heavy chain of human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA (Jefferis et al. 1985).
Elevated levels of IgG4 and of IgG4 presenting plasma cells are frequently seen in patients with autoimmune pancreatitis (IAP) and inflammatory bowel disease (Navaneethan et al. 2011) and it is suggested that IAP may develop as a paraneoplastic syndrome in some cancer patients (Shiokawa et al. 2013)
Elevated levels of IgG4 and of IgG4 presenting plasma cells are frequently seen in patients with autoimmune pancreatitis (IAP) and inflammatory bowel disease (Navaneethan et al. 2011) and it is suggested that IAP may develop as a paraneoplastic syndrome in some cancer patients (Shiokawa et al. 2013)
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified IgG4
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with SP2/0 - Ag14 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P01861 Related reagents
- Entrez Gene
- IGHG4 Related reagents
- GO Terms
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IgG4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/25600 | 1/51200 | |
| Immunohistology - Frozen | 1/40 | 1/80 | |
| Immunohistology - Paraffin | |||
| ELISA | |||
| Immunoblotting | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Tonsil
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E |
Product Specific References
References for IgG4 antibody
-
Jefferis, R. et al. (1985) Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study.
Immunol Lett. 10 (3-4): 223-52. -
Agaimy, A. et al. (2010) Calcifying fibrous tumor of the stomach: clinicopathologic and molecular study of seven cases with literature review and reappraisal of histogenesis.
Am J Surg Pathol. 34: 271-8. -
Strehl, J.D. et al. (2011) Numerous IgG4-positive plasma cells are ubiquitous in diverse localised non-specific chronic inflammatory conditions and need to be distinguished from IgG4-related systemic disorders.
J Clin Pathol. 64 (3): 237-43. -
Black, C.M. et al. (1991) Human markers for IgG2 and IgG4 appear to be on the same molecule in the chimpanzee.
Immunology.72: 94-8. -
Fernandez-Becerra, C. (2010) Naturally-acquired humoral immune responses against the N- and C-termini of the Plasmodium vivax MSP1 protein in endemic regions of Brazil and Papua New Guinea using a multiplex assay.
Malar J. 9: 29. -
Lee, H.W. et al. (2008) High levels of antibodies to Plasmodium falciparum liver stage antigen-1 in naturally infected individuals in Myanmar.
Korean J Parasitol. 46: 195-8. -
Shiokawa, M. et al. (2013) Risk of cancer in patients with autoimmune pancreatitis.
Am J Gastroenterol. 108 (4): 610-7. -
Whelan, S.F. et al. (2013) Distinct characteristics of antibody responses against factor VIII in healthy individuals and in different cohorts of hemophilia A patients.
Blood. 121: 1039-48. -
Fujimoto, M. et al. (2013) Stromal plasma cells expressing immunoglobulin G4 subclass in non-small cell lung cancer.
Hum Pathol. 44 (8): 1569-76. -
Miyagawa-Hayashino, A. et al. (2009) High ratio of IgG4-positive plasma cell infiltration in cutaneous plasmacytosis--is this a cutaneous manifestation of IgG4-related disease?
Hum Pathol. 40: 1269-77. -
Yamashita, K. et al. (2008) Lung involvement in IgG4-related lymphoplasmacytic vasculitis and interstitial fibrosis: report of 3 cases and review of the literature.
Am J Surg Pathol. 32: 1620-6. -
Yamashita, K. et al. (2008) Degree of IgG4+ plasma cell infiltration in retroperitoneal fibrosis with or without multifocal fibrosclerosis
Histopathology. 52: 404-9. -
Navaneethan, U. et al. (2011) Tissue infiltration of IgG4+ plasma cells in symptomatic patients with ileal pouch-anal anastomosis
J Crohns Colitis. 5: 570-6. -
Shiokawa, M. et al. (2013) Risk of cancer in patients with autoimmune pancreatitis.
Am J Gastroenterol. 108 (4): 610-7. -
Engelmann, R. et al. (2015) Bone resorption correlates with the frequency of CD5⁺ B cells in the blood of patients with rheumatoid arthritis.
Rheumatology (Oxford). 54 (3): 545-53. -
Ráty, S. et al. (2015) Tumor-like Chronic Pancreatitis Is Often Autoimmune Pancreatitis.
Anticancer Res. 35 (11): 6163-6. -
Agaimy, A. et al. (2015) SMARCA4-deficient undifferentiated carcinoma of the ovary (small cell carcinoma, hypercalcemic type): clinicopathologic and immunohistochemical study of 3 cases.
Ann Diagn Pathol. 19 (5): 283-7. -
Riedemann, N.C. et al. (2017) Controlling the anaphylatoxin C5a in diseases requires a specifically targeted inhibition.
Clin Immunol. 180: 25-32. -
Pan, Q. et al. (2016) Association between IgG4 Autoantibody and Complement Abnormalities in Systemic Lupus Erythematosus.
Mediators Inflamm. 2016: 2196986.
