IgG4 antibody | HP6025
Mouse anti Human IgG4
- Product Type
- Monoclonal Antibody
|Mouse anti Human IgG4 antibody, clone HP6025 recognizes the heavy chain of human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA (Jefferis et al. 1985).
Elevated levels of IgG4 and of IgG4 presenting plasma cells are frequently seen in patients with autoimmune pancreatitis (IAP) and inflammatory bowel disease (Navaneethan et al. 2011) and it is suggested that IAP may develop as a paraneoplastic syndrome in some cancer patients (Shiokawa et al. 2013)
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Chimpanzee
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antibody purified from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Purified IgG4
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with SP2/0 - Ag14 mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
References for IgG4 antibody
Jefferis, R. et al. (1985) Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study.
Immunol Lett. 10 (3-4): 223-52.
Black, C.M. et al. (1991) Human markers for IgG2 and IgG4 appear to be on the same molecule in the chimpanzee.
Yamashita, K. et al. (2008) Degree of IgG4+ plasma cell infiltration in retroperitoneal fibrosis with or without multifocal fibrosclerosis
Histopathology. 52: 404-9.
Yamashita, K. et al. (2008) Lung involvement in IgG4-related lymphoplasmacytic vasculitis and interstitial fibrosis: report of 3 cases and review of the literature.
Am J Surg Pathol. 32: 1620-6.
Miyagawa-Hayashino, A. et al. (2009) High ratio of IgG4-positive plasma cell infiltration in cutaneous plasmacytosis--is this a cutaneous manifestation of IgG4-related disease?
Hum Pathol. 40: 1269-77.
Fernandez-Becerra, C. (2010) Naturally-acquired humoral immune responses against the N- and C-termini of the Plasmodium vivax MSP1 protein in endemic regions of Brazil and Papua New Guinea using a multiplex assay.
Malar J. 9: 29.
Agaimy, A. et al. (2010) Calcifying fibrous tumor of the stomach: clinicopathologic and molecular study of seven cases with literature review and reappraisal of histogenesis.
Am J Surg Pathol. 34: 271-8.
Navaneethan, U. et al. (2011) Tissue infiltration of IgG4+ plasma cells in symptomatic patients with ileal pouch-anal anastomosis
J Crohns Colitis. 5: 570-6.
View The Latest Product References
Strehl, J.D. et al. (2011) Numerous IgG4-positive plasma cells are ubiquitous in diverse localised non-specific chronic inflammatory conditions and need to be distinguished from IgG4-related systemic disorders.
J Clin Pathol. 64 (3): 237-43.
Shiokawa, M. et al. (2013) Risk of cancer in patients with autoimmune pancreatitis.
Am J Gastroenterol. 108 (4): 610-7.
Whelan, S.F. et al. (2013) Distinct characteristics of antibody responses against factor VIII in healthy individuals and in different cohorts of hemophilia A patients.
Blood. 121: 1039-48.
Fujimoto, M. et al. (2013) Stromal plasma cells expressing immunoglobulin G4 subclass in non-small cell lung cancer.
Hum Pathol. 44 (8): 1569-76.
Engelmann, R. et al. (2015) Bone resorption correlates with the frequency of CD5⁺ B cells in the blood of patients with rheumatoid arthritis.
Rheumatology (Oxford). 54 (3): 545-53.
Ráty, S. et al. (2015) Tumor-like Chronic Pancreatitis Is Often Autoimmune Pancreatitis.
Anticancer Res. 35 (11): 6163-6.
Agaimy, A. et al. (2015) SMARCA4-deficient undifferentiated carcinoma of the ovary (small cell carcinoma, hypercalcemic type): clinicopathologic and immunohistochemical study of 3 cases.
Ann Diagn Pathol. 19 (5): 283-7.
Pan, Q. et al. (2016) Association between IgG4 Autoantibody and Complement Abnormalities in Systemic Lupus Erythematosus.
Mediators Inflamm. 2016: 2196986.
Riedemann, N.C. et al. (2017) Controlling the anaphylatoxin C5a in diseases requires a specifically targeted inhibition.
Clin Immunol. 180: 25-32.
Engelmann, R. et al. (2018) Decreased IgG4 ACPA levels in responders and increased CD1c+ classical dendritic cells in non-responders of patients with rheumatoid arthritis under therapy.
Clin Rheumatol. 37 (7): 1783-90.
Derakhshandeh, R. et al. (2021) Single Institutional Experience on Orbital Inflammatory Pseudotumor: Diagnostic and Management Challenge.
Balkan Med J. 38 (4): 239-43.
Agaimy, A. et al. (2023) Paravertebral fibrous pseudotumor: Four cases of a distinctive tumefactive lesion overlapping with eosinophilic angiocentric fibrosis and tumoral erythema elevatum diutinum.
Ann Diagn Pathol. 62: 152073.
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