IgG antibody | MK 1 A6
Mouse anti Human IgG (Fc) CH2 domain antibody, clone MK 1 A6 used for the detection of CH2 by flow cytometry.
Image caption:
(A) Selection strategy for the generation of pH-dependent binders. A yeast displayed Fcab library was incubated with 3 nM biotinylated Her2-ECD, followed by labeling with SA-PE and anti-CH2-FITC. The incubations were either performed at pH 7.4 (selection rounds 1, 2, and 5) or at pH 6.0 (rounds 3, 4, and 6). If the staining procedure was done at pH 7.4, the cells were subsequently sorted for binding to Her2, whereas incubation at pH 6.0 was followed by selection of non-binders. Exemplarily, the dot plots from selection rounds 4 (top) and 5 (bottom) are shown. The boxes within the plots indicate the gates that were used for sorting. (B) Analysis of the pH-dependence of Her2-binding of selected Fcab mutants. After six rounds of flow cytometric sorting, individual Fcab-clones were analyzed. Fcabs were displayed on yeast and incubated with 3 nM biotinylated Her2, followed by detection of antigen binding with SA-PE and of the surface display level with anti-Xpress-APC (recognizing an N-terminal expression tag located between Aga2p and the Fcab). Incubation steps were either performed at pH 6.0 (gray lines) or 7.4 (black lines). Only Xpress-positive (i.e. displaying) cells were analyzed. In total, 10 Fcabs that have been enriched during selection were tested for pH-dependent Her2 binding. Only the three best performing clones (P1, P2, and P3) are shown.
From: Traxlmayr, M. W., Lobner, E., Hasenhindl, C., Stadlmayr, G., Oostenbrink, C., Rüker, F. and Obinger, C. (2014), Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution. Biotechnology Journal, 9: 1013–1022.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human IgG (Fc) CH2 Domain
- Product Type
- Monoclonal Antibody
- Clone
- MK 1 A6
- Isotype
- IgG1
- Specificity
- IgG
- Region
- (Fc) (CH2 DOMAIN)
Antibody Quality and Performance Guarantee
| Mouse anti Human IgG (Fc) CH2 domain, clone MK 1 A6 recognizes human IgG Fc (all subclasses). CH2 and hinge regions have an important role in effector functions of IgG. The epitope detected by clone MK 1 A6 lies within the CH2 domain as determined by haemagglutination and western blotting using IgG heavy chain and myelomas with defined domain deletions. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human IgG Polyclonal.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from BALB/c mouse were fused with cells from the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/1,000 | 1/10,000 |
| Immunohistology - Frozen 1 | |
||
| Immunohistology - Paraffin | |||
| Western Blotting | |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgG antibody
-
Lund, J. et al. (1996) Multiple interactions of IgG with its core oligosaccharide can modulate recognition by complement and human Fc gamma receptor I and influence the synthesis of its oligosaccharide chains.
J Immunol. 157 (11): 4963-9. -
Rasti, N. et al. (2006) Nonimmune immunoglobulin binding and multiple adhesion characterize Plasmodium falciparum-infected erythrocytes of placental origin.
Proc Natl Acad Sci U S A. 103: 13795-800. -
Wozniak-Knopp, G. et al. (2010) Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties.
Protein Eng Des Sel. 23: 289-97. -
Raghuraman, S. et al. (2012) Spontaneous clearance of chronic hepatitis C virus infection is associated with appearance of neutralizing antibodies and reversal of T-cell exhaustion.
J Infect Dis. 205: 763-71. -
Hasenhindl, C. et al. (2013) Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.
Protein Eng Des Sel. 26 (10): 675-82. -
Traxlmayr, M.W. et al. (2014) Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution.
Biotechnol J. 9: 1013-22. -
Bali, N. et al. (2022) Sticks and Stones, a conserved cell surface ligand for the Type IIa RPTP Lar, regulates neural circuit wiring in Drosophila.
Elife. 11: e71469.
- RRID
- AB_321910
- UniProt
- P01857
- P01859
- P01861
- P01834
- P01860
- P0CG04
- Entrez Gene
- IGKC
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- IGLV2-14
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
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