IgG antibody | MK 1 A6

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Anti Human IgG (Fc) CH2 Domain Antibody, clone MK 1 A6 thumbnail image 1

Anti Human IgG (Fc) CH2 Domain Antibody, clone MK 1 A6 thumbnail image 2

Anti Human IgG (Fc) CH2 Domain Antibody, clone MK 1 A6 gallery image 1 — Detection of Human Fc (CH2) using HRP conjugated Mouse anti Human Fc specific antibody (**MCA647P**) in an Adalimumab sensitivity ELISA. Human anti Adalimumab (HCA202) was used as the coating antibody followed by Adalimumab (D2E7) at the given concentrations as the antigen.

Anti Human IgG (Fc) CH2 Domain Antibody, clone MK 1 A6 gallery image 2 — **Published customer image**
Mouse anti Human IgG (Fc) CH2 domain antibody, clone MK 1 A6 used for the detection of CH2 by flow cytometry.
**Image caption:**
(A) Selection strategy for the generation of pH-dependent binders. A yeast displayed Fcab library was incubated with 3 nM biotinylated Her2-ECD, followed by labeling with SA-PE and anti-CH2-FITC. The incubations were either performed at pH 7.4 (selection rounds 1, 2, and 5) or at pH 6.0 (rounds 3, 4, and 6). If the staining procedure was done at pH 7.4, the cells were subsequently sorted for binding to Her2, whereas incubation at pH 6.0 was followed by selection of non-binders. Exemplarily, the dot plots from selection rounds 4 (top) and 5 (bottom) are shown. The boxes within the plots indicate the gates that were used for sorting. (B) Analysis of the pH-dependence of Her2-binding of selected Fcab mutants. After six rounds of flow cytometric sorting, individual Fcab-clones were analyzed. Fcabs were displayed on yeast and incubated with 3 nM biotinylated Her2, followed by detection of antigen binding with SA-PE and of the surface display level with anti-Xpress-APC (recognizing an N-terminal expression tag located between Aga2p and the Fcab). Incubation steps were either performed at pH 6.0 (gray lines) or 7.4 (black lines). Only Xpress-positive (i.e. displaying) cells were analyzed. In total, 10 Fcabs that have been enriched during selection were tested for pH-dependent Her2 binding. Only the three best performing clones (P1, P2, and P3) are shown.

From: Traxlmayr, M. W., Lobner, E., Hasenhindl, C., Stadlmayr, G., Oostenbrink, C., Rüker, F. and Obinger, C. (2014), Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution. Biotechnology Journal, 9: 1013–1022.

Mouse anti Human IgG (Fc) CH2 Domain:FITC

Mouse anti Human IgG (Fc) CH2 Domain:HRP

Mouse anti Human IgG (Fc) CH2 Domain

Product Type: Monoclonal Antibody
Clone: MK 1 A6
Isotype: IgG1

Product Code	Applications	Pack Size
MCA647F	E F	0.1 mg
MCA647P	C* E	0.2 mg
MCA647G	C* E WB	0.2 mg

Mouse anti Human IgG (Fc) CH2 domain, clone MK 1 A6 recognizes human IgG Fc (all subclasses).

CH2 and hinge regions have an important role in effector functions of IgG. The epitope detected by clone MK 1 A6 lies within the CH2 domain as determined by haemagglutination and western blotting using IgG heavy chain and myelomas with defined domain deletions.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rhesus Monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Prosep A

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.01% Thiomersal

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Human IgG Polyclonal.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from BALB/c mouse were fused with cells from the mouse NS1 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01857 Related reagents; P01859 Related reagents; P01861 Related reagents; P01834 Related reagents; P01860 Related reagents; P0CG04 Related reagents
Entrez Gene: IGKC Related reagents; IGHG1 Related reagents; IGHG2 Related reagents; IGHG3 Related reagents; IGHG4 Related reagents; IGLV2-14 Related reagents
GO Terms: GO:0005515 protein binding; GO:0003823 antigen binding; GO:0005576 extracellular region; GO:0006958 complement activation, classical pathway; GO:0045087 innate immune response; GO:0005624 membrane fraction; GO:0006955 immune response
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/1000	1/10000
Flow Cytometry	Neat	1/10
ELISA	1/1000	1/10000
Immunohistology - Frozen ¹	1/100	1/200
Immunohistology - Paraffin
ELISA	1/1,000	1/10,000
Immunohistology - Frozen ¹
Immunohistology - Paraffin
Western Blotting

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
AbGUARD® HRP Stabilizer Plus	BUF052A	100 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052B	500 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052C	1000 ml	C E P WB
TMB Signal+	BUF054A	100 ml	E
TMB Core	BUF056A	100 ml	E
TMB Core+	BUF062A	100 ml	E

Application Based External Images

ELISA

Flow Cytometry

Product Specific References

References for IgG antibody

Lund, J. et al. (1996) Multiple interactions of IgG with its core oligosaccharide can modulate recognition by complement and human Fc gamma receptor I and influence the synthesis of its oligosaccharide chains.
J Immunol. 157 (11): 4963-9.
Wozniak-Knopp, G. et al. (2010) Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties.
Protein Eng Des Sel. 23: 289-97.
Raghuraman, S. et al. (2012) Spontaneous clearance of chronic hepatitis C virus infection is associated with appearance of neutralizing antibodies and reversal of T-cell exhaustion.
J Infect Dis. 205: 763-71.
Hasenhindl, C. et al. (2013) Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.
Protein Eng Des Sel. 26 (10): 675-82.
Rasti, N. et al. (2006) Nonimmune immunoglobulin binding and multiple adhesion characterize Plasmodium falciparum-infected erythrocytes of placental origin.
Proc Natl Acad Sci U S A. 103: 13795-800.
Traxlmayr, M.W. et al. (2014) Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution.
Biotechnol J. 9: 1013-22.

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