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IgG antibody | MK 1 A6

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Anti Human IgG (Fc) CH2 Domain Antibody, clone MK 1 A6 thumbnail image 1 Anti Human IgG (Fc) CH2 Domain Antibody, clone MK 1 A6 thumbnail image 2
Anti Human IgG (Fc) CH2 Domain Antibody, clone MK 1 A6 gallery image 1
Detection of Human Fc (CH2) using HRP conjugated Mouse anti Human Fc specific antibody (MCA647P) in an Adalimumab sensitivity ELISA. Human anti Adalimumab (HCA202) was used as the coating antibody followed by Adalimumab (D2E7) at the given concentrations as the antigen.
Anti Human IgG (Fc) CH2 Domain Antibody, clone MK 1 A6 gallery image 2
Published customer image
Mouse anti Human IgG (Fc) CH2 domain antibody, clone MK 1 A6 used for the detection of CH2 by flow cytometry.
Image caption:
(A) Selection strategy for the generation of pH-dependent binders. A yeast displayed Fcab library was incubated with 3 nM biotinylated Her2-ECD, followed by labeling with SA-PE and anti-CH2-FITC. The incubations were either performed at pH 7.4 (selection rounds 1, 2, and 5) or at pH 6.0 (rounds 3, 4, and 6). If the staining procedure was done at pH 7.4, the cells were subsequently sorted for binding to Her2, whereas incubation at pH 6.0 was followed by selection of non-binders. Exemplarily, the dot plots from selection rounds 4 (top) and 5 (bottom) are shown. The boxes within the plots indicate the gates that were used for sorting. (B) Analysis of the pH-dependence of Her2-binding of selected Fcab mutants. After six rounds of flow cytometric sorting, individual Fcab-clones were analyzed. Fcabs were displayed on yeast and incubated with 3 nM biotinylated Her2, followed by detection of antigen binding with SA-PE and of the surface display level with anti-Xpress-APC (recognizing an N-terminal expression tag located between Aga2p and the Fcab). Incubation steps were either performed at pH 6.0 (gray lines) or 7.4 (black lines). Only Xpress-positive (i.e. displaying) cells were analyzed. In total, 10 Fcabs that have been enriched during selection were tested for pH-dependent Her2 binding. Only the three best performing clones (P1, P2, and P3) are shown.

From: Traxlmayr, M. W., Lobner, E., Hasenhindl, C., Stadlmayr, G., Oostenbrink, C., Rüker, F. and Obinger, C. (2014), Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution. Biotechnology Journal, 9: 1013–1022.

Mouse anti Human IgG (Fc) CH2 Domain:FITC

Mouse anti Human IgG (Fc) CH2 Domain:HRP

Mouse anti Human IgG (Fc) CH2 Domain

Product Type
Monoclonal Antibody
Clone
MK 1 A6
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA647F
Datasheet
E F
SDS
0.1 mg loader

MCA647P
Datasheet
C* E
SDS
0.2 mg loader

MCA647G
Datasheet
C* E WB
SDS
0.2 mg loader

Mouse anti Human IgG (Fc) CH2 domain, clone MK 1 A6 recognizes human IgG Fc (all subclasses).

CH2 and hinge regions have an important role in effector functions of IgG. The epitope detected by clone MK 1 A6 lies within the CH2 domain as determined by haemagglutination and western blotting using IgG heavy chain and myelomas with defined domain deletions.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Prosep A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human IgG Polyclonal.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from BALB/c mouse were fused with cells from the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01857
P01859
P01861
P01834
P01860
P0CG04
Entrez Gene
IGKC
IGHG1
IGHG2
IGHG3
IGHG4
IGLV2-14
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Flow Cytometry Neat 1/10
ELISA 1/1000 1/10000
Immunohistology - Frozen 1 1/100 1/200
Immunohistology - Paraffin
ELISA 1/1,000 1/10,000
Immunohistology - Frozen 1
Immunohistology - Paraffin
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Application Based External Images

ELISA

Flow Cytometry

Product Specific References

References for IgG antibody

  1. Lund, J. et al. (1996) Multiple interactions of IgG with its core oligosaccharide can modulate recognition by complement and human Fc gamma receptor I and influence the synthesis of its oligosaccharide chains.
    J Immunol. 157 (11): 4963-9.
  2. Wozniak-Knopp, G. et al. (2010) Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties.
    Protein Eng Des Sel. 23: 289-97.
  3. Raghuraman, S. et al. (2012) Spontaneous clearance of chronic hepatitis C virus infection is associated with appearance of neutralizing antibodies and reversal of T-cell exhaustion.
    J Infect Dis. 205: 763-71.
  4. Hasenhindl, C. et al. (2013) Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.
    Protein Eng Des Sel. 26 (10): 675-82.
  5. Rasti, N. et al. (2006) Nonimmune immunoglobulin binding and multiple adhesion characterize Plasmodium falciparum-infected erythrocytes of placental origin.
    Proc Natl Acad Sci U S A. 103: 13795-800.
  6. Traxlmayr, M.W. et al. (2014) Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution.
    Biotechnol J. 9: 1013-22.

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