IgG antibody | B33/20

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Mouse anti Human IgG

Product Type: Monoclonal Antibody
Clone: B33/20
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
5172-6004	E			1 mg

Mouse anti Human IgG antibody, clone B33/20 is reactive with all subclasses of Gamma heavy chain (Cgamma-2).

Mouse anti Human IgG antibody, clone B33/20 has not been species cross adsorbed and may cross react with immunoglobulins from other species.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by ProSep G chromatography

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Purified human IgG.

Approx. Protein Concentrations

1.0 mg/ml

Fusion Partners

Spleen cells from immunised Balb/C mice were fused with cells of the Sp2/O-Ag14 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01857 Related reagents; P01859 Related reagents; P01861 Related reagents; P01857 Related reagents; P01859 Related reagents; P01861 Related reagents; P01860 Related reagents; P01860 Related reagents
Entrez Gene: IGHG1 Related reagents; IGHG2 Related reagents; IGHG3 Related reagents; IGHG4 Related reagents
GO Terms: GO:0005515 protein binding; GO:0003823 antigen binding; GO:0005576 extracellular region; GO:0005624 membrane fraction; GO:0006955 immune response
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for IgG antibody

Poussin C et al. (2014) Mechanism of an indirect effect of aqueous cigarette smoke extract on the adhesion of monocytic cells to endothelial cells in an in vitro assay revealed by transcriptomics analysis.
Toxicol In Vitro. 28 (5): 896-908.

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