IgA1 antibody | B3506B4
HRP conjugated Mouse anti Human IgA1 antibody, clone B3506B4 (913005) used for the evaluation of IgA1 levels in human serum samples by ELISA.
Image caption:
Comparison of serum IgA in patients with IgA nephropathy and heathy subjects. J-IgA: joining chain-containing IgA; J-IgA1: joining chain-containing IgA1; IgAN: IgA nephropathy; NC: normal control. Statistically significant differences were tested with Mann–Whitney U-test.
From: Li G, Wang X, Yang Z, Zhao Q, Wen Y, Li X, Gao R.
Serum Levels of Joining Chain-Containing IgA1 Are Not Elevated in Patients with IgA Nephropathy.
Dis Markers. 2019 Jul 2;2019:9802839.
doi: 10.1155/2019/9802839.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
HRP conjugated Mouse anti Human IgA1 antibody, clone B3506B4 (913005) used for the evaluation of IgA1 levels in plasma samples by western blotting.
Image caption:
Verification of successful preparation of ddIgA1 and IgG-dd-IgA1 complexes. a Glycosylation status of IgA1 and ddIgA1 were detected by two lectins, SNA and VVL, which recognized sialic acid (SA), and N-Acetylgalactosamine (GalNAc), respectively. IgA1 molecules showed high binding capacity to SNA (blank column) and low binding capacity to VVL (filled column). In contrary, ddIgA1 showed low binding capacity to SNA (blank column) and high binding capacity to VVL (filled column). b Existence of dd-IgA1 (upper panel) and IgG (lower panel) in IgG-ddIgA1 complexes were tested by Western blot analysis. Both IgA1 and IgG were existence in IgG-ddIgA1 complexes of recruited individuals (lane 1–4). Isolated ddIgA1 (lane 5), IgA1 (lane 6) and IgG (lane 7) were used as references
From: Zhao YF, Zhu L, Liu LJ, Shi SF, Lv JC, Zhang H.
Pathogenic role of glycan-specific IgG antibodies in IgA nephropathy.
BMC Nephrol. 2017 Sep 29;18(1):301.
doi: 10.1186/s12882-017-0722-3.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human IgA1 Alpha 1 Chain
- Product Type
- Monoclonal Antibody
- Clone
- B3506B4
- Isotype
- IgG1
- Specificity
- IgA1
Antibody Quality and Performance Guarantee
| Mouse anti Human IgA1 antibody, clone B3506B4 recognizes the Fc portion of the heavy chain of human IgA1 as demonstrated by ELISA. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Human IgA1 heavy chains.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunoblotting | |
||
| Immunohistology - Frozen | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for IgA1 antibody
-
Zhao, Y.F. et al. (2017) Pathogenic role of glycan-specific IgG antibodies in IgA nephropathy.
BMC Nephrol. 18 (1): 301. -
Li, G. et al. (2019) Serum Levels of Joining Chain-Containing IgA1 Are Not Elevated in Patients with IgA Nephropathy.
Dis Markers. 2019: 9802839.
- RRID
- AB_620166
- UniProt
- P01876
- Entrez Gene
- IGHA1
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0006955 immune response
- GO:0018298 protein-chromophore linkage
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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