iC3b antibody | 013III-1.16
iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified human iC3b
- Approx. Protein Concentrations
- Please see label for concentration
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P01024
- Entrez Gene
- C3
- GO Terms
- GO:0005102 receptor binding
- GO:0004866 endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0010575 positive regulation vascular endothelial growth factor production
- Regulatory
- For research purposes only
Applications of iC3b antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Flow Cytometry | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin | 10 ug/ml | ||
Western Blotting |
- Histology Positive Control Tissue
- Kidney from post streptoccal glomerulonephritis patients
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Histar Detection System | STAR3000A | C P | 50 Tests |
Product Specific References
Further Reading
-
Janssena, B. et al. (2007) Structural insights into the central complement component C3.
Molecular Immunology. 44: 3-10. -
Bergmann-Leitner, E.S. et al. (2006) Complement 3d: from molecular adjuvant to target of immune escape mechanisms.
Clin Immunol. 121 (2): 177-85. -
Sohn, J.H. et al. (2003) Tolerance is dependent on complement C3 fragment iC3b binding to antigen-presenting cells.
Nat Med. 9 (2): 206-12.
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