HLA B7 antibody | BB7.1

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Anti Human HLA B7 Antibody, clone BB7.1 thumbnail image 1

Anti Human HLA B7 Antibody, clone BB7.1 thumbnail image 2

Anti Human HLA B7 Antibody, clone BB7.1 gallery image 1 — Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B . Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse anti Human HLA-B7 (MCA986A647). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer

Anti Human HLA B7 Antibody, clone BB7.1 gallery image 2 — Figure A. PE conjugated Mouse anti Human CD3 (MCA463PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B . PE conjugated Mouse anti Human CD3 (MCA463F) and FITC conjugated Mouse anti Human HLA-B7 (MCA986F). All experiments performed on human blood gated on single cell lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human HLA B7:Alexa Fluor® 647

Mouse anti Human HLA B7:FITC

Mouse anti Human HLA B7

Mouse anti Human HLA B7:RPE

Product Type: Monoclonal Antibody
Clone: BB7.1
Isotype: IgG1

Product Code	Applications	Pack Size
MCA986A647	F	100 Tests/1ml
MCA986F	F	0.1 mg
MCA986	F	0.2 mg
MCA986PE	F	100 Tests

Mouse anti Human HLA B7 antibody, clone BB7.1 recognizes the HLA B7 antigen and does not cross-react with HLA B27 or other related antigens. It can be used to distinguish true HLA B27 positives from false HLA B27 positives (i.e. HLA B7 positive) in the investigation of diseases such as ankylosing spondylitis and anterior uveitis. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C.

The HLA B gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Cynomolgus monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alexa Fluor 647 - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1 ml distilled water.

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Preparation

Purified IgG prepared by affinity chromatography on Protein A.

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Immunogen

Papain solubilized HLA-B7 antigen.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred. Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

More Information

UniProt: P01889 Related reagents
Entrez Gene: HLA-B Related reagents
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of HLA B7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry		Neat
Flow Cytometry	Neat
Flow Cytometry		1/50
Flow Cytometry		Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 10⁶ cells or 100ul whole blood
Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 10⁶ cells or 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 10⁶ cells or 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 10⁶ cells or 100ul whole blood.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Product Specific References

References for HLA B7 antibody

Brodsky, F.M. et al. (1979) Monoclonal antibodies for analysis of the HLA system.
Immunol Rev. 47: 3-61.
Yoshino N et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp Anim. 49 (2): 97-110.
Anania, V.G. & Coscoy, L. (2011) Palmitoylation of MIR2 is required for its function.
J Virol. 85 (5): 2288-95.
Bonaparte, M.I. and Barker, E. (2004) Killing of human immunodeficiency virus-infected primary T-cell blasts by autologous natural killer cells is dependent on the ability of the virus to alter the expression of major histocompatibility complex class I molecules.
Blood. 104: 2087-94.
Dellgren, C. et al. (2016) Low Constitutive Cell Surface Expression of HLA-B Is Caused by a Posttranslational Mechanism Involving Glu180 and Arg239.
J Immunol. 197 (12): 4807-4816.

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