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HLA B7 antibody | BB7.1

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Anti Human HLA B7 Antibody, clone BB7.1 thumbnail image 1 Anti Human HLA B7 Antibody, clone BB7.1 thumbnail image 2 Anti Human HLA B7 Antibody, clone BB7.1 thumbnail image 3
Anti Human HLA B7 Antibody, clone BB7.1 gallery image 1
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and Amethyst Orange conjugated Mouse IgG1 isotype control (MCA928AMO). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and Amethyst Orange conjugated Mouse anti Human HLA B7 (MCA986AMO). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human HLA B7 Antibody, clone BB7.1 gallery image 2
Figure A. PE conjugated Mouse anti Human CD3 (MCA463PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B . PE conjugated Mouse anti Human CD3 (MCA463F) and FITC conjugated Mouse anti Human HLA-B7 (MCA986F). All experiments performed on human blood gated on single cell lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human HLA B7 Antibody, clone BB7.1 gallery image 3
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B . Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Alexa Fluor 647 conjugated Mouse anti Human HLA-B7 (MCA986A647). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human HLA B7:FITC

Product Type
Monoclonal Antibody
Clone
BB7.1
Isotype
IgG1
Specificity
HLA B7

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Product Code Applications Pack Size List Price Your Price Qty
MCA986F
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SDS Safety Datasheet SDS
F 0.1 mg loader
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Mouse anti Human HLA B7 antibody, clone BB7.1 recognizes the HLA B7 antigen and does not cross-react with HLA B27 or other related antigens. It can be used to distinguish true HLA B27 positives from false HLA B27 positives (i.e. HLA B7 positive) in the investigation of diseases such as ankylosing spondylitis and anterior uveitis. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C.

The HLA B gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Papain solubilized HLA-B7 antigen
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells or 100ul whole blood.

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Description Mouse IgG1 Negative Control:FITC

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References for HLA B7 antibody

  1. Brodsky, F.M. et al. (1979) Monoclonal antibodies for analysis of the HLA system.
    Immunol Rev. 47: 3-61.
  2. Bonaparte, M.I. and Barker, E. (2004) Killing of human immunodeficiency virus-infected primary T-cell blasts by autologous natural killer cells is dependent on the ability of the virus to alter the expression of major histocompatibility complex class I molecules.
    Blood. 104: 2087-94.
  3. Anania, V.G. & Coscoy, L. (2011) Palmitoylation of MIR2 is required for its function.
    J Virol. 85 (5): 2288-95.
  4. Dellgren, C. et al. (2016) Low Constitutive Cell Surface Expression of HLA-B Is Caused by a Posttranslational Mechanism Involving Glu180 and Arg239.
    J Immunol. 197 (12): 4807-16.

Flow Cytometry

RRID
AB_323701
UniProt
P01889
Entrez Gene
HLA-B

MCA986F

162175 1703

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