Glucocorticoid Receptor antibody | 5E4
Mouse anti Human glucocorticoid receptor antibody, clone 5E4 (MCA2469) used for the detection of GCR expression on NKT-like cells from patients with COPD by flow cytometry.
Image caption:
The percentage of CD28+ and CD28null CD8+ (clear bars) and CD8- NKT-like cells expressing GCR in patients with COPD.a. The percentage of CD28+ and CD28null CD8+ (clear bars) and CD8- T cells expressing GCR in patients with COPD. Data presented as box plots. There was a significant decrease in the percentage of CD28null/CD8- and CD28nullCD8+ T cells expressing GCR compared with CD28 + CD8- and CD28 + CD8+ T cells. There was a significant decrease in the percentage of CD8 + CD28null T cells expressing GCR compared with CD8-CD28null T cells. b. The percentage of CD28+ and CD28null CD8+ (clear bars) and CD8- NKT-like cells expressing GCR in patients with COPD. Data presented as box plots. There was a significant decrease in the percentage of CD28null/CD8- T and CD28nullCD8+ NKT-like cells expressing GCR compared with CD28 + CD8- T and CD28 + CD8+ NKT-like cells. There was a significant decrease in the percentage of CD8 + CD28null NKT-like cells expressing GCR compared with CD8-CD28null T cells.
From: Hodge G, Jersmann H, Tran HB, Holmes M, Reynolds PN, Hodge S. Lymphocyte senescence in COPD is associated with loss of glucocorticoid receptor expression by pro-inflammatory/cytotoxic lymphocytes.
Respir Res. 2015 Jan 9;16(1):2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human glucocorticoid receptor antibody, clone 5E4 (MCA2469) used for the detection of GCR expression on NKT-like cells from patients with COPD by immunofluorescence.
Image caption:
Representative immunofluorescence microphotos of GCR staining in sorted CD28null (left) and CD28+ T cells (middle). Magenta is merged color of red (GCR) and blue (DAPI). Mean fluorescence intensity measured by ImageJ software was 160.5 ± 9.3 in CD28null and 336.2 ± 25.0 in CD28+ cells (p < 0.001). Experiments were repeated 2 times, showing similar results.
From: Hodge G, Jersmann H, Tran HB, Holmes M, Reynolds PN, Hodge S. Lymphocyte senescence in COPD is associated with loss of glucocorticoid receptor expression by pro-inflammatory/cytotoxic lymphocytes.
Respir Res. 2015 Jan 9;16(1):2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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- Product Type
- Monoclonal Antibody
- Clone
- 5E4
- Isotype
- IgG1
- Specificity
- Glucocorticoid Receptor
Antibody Quality and Performance Guarantee
| Mouse anti Human Glucocorticoid Receptor antibody, clone 5E4 recognizes the human glucocorticoid receptor, also known as Nuclear receptor subfamily 3 group C member 1 (NR3C1), a 777 amino acid receptor bearing 3 distinct functional domains, an N-terminal modulating domain, a DNA binding domain and a C-terminal steroid binding domain. The human glucocorticoid receptor is located either in the cytoplasm of cells, prior to ligand binding associated with certain heat shock proteins (Kino et al. 2009), or the nucleus following binding to ligand (Werb et al. 1978). Mutations in the glucocorticoid receptor gene can lead to familial glucocorticoid resistance, characterized by elevated plasma cortisol levels (Malchoff et al. 1993). Mouse anti human glucocorticoid receptor antibody, clone 5E4 has been demonstrated to cross-react with the murine glucocorticoid receptor (Bergquist et al. 2014) |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
0.1% Bovine Serum Albumin - Immunogen
- 26 amino acid peptide corresponding to residues 150-176 on human GCR linked to thyroglobulin.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp-2/0 Ag14 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
Neat | 1/10 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for Glucocorticoid Receptor antibody
-
Berki, T. et al. (1998) Production and flow cytometric application of a monoclonal anti-glucocorticoid receptor antibody.
J Immunol Methods. 214 (1-2): 19-27. -
Kim, S.Y. et al. (2010) Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line
Korean J Pediatr 53: 753-8 -
Bergquist, M. et al. (2014) Glucocorticoid receptor function is decreased in neutrophils during endotoxic shock.
J Infect. pii: S0163-4453(14)00081-4. -
Hodge, G. et al. (2015) Lymphocyte senescence in COPD is associated with loss of glucocorticoid receptor expression by pro-inflammatory/cytotoxic lymphocytes.
Respir Res. 16 (1): 2. -
Bergquist, M. et al. (2016) Glucocorticoid receptor expression and binding capacity in patients with burn injury.
Acta Anaesthesiol Scand. 60 (2): 213-21. -
Bergquist, M. et al. (2015) Impairment of neutrophilic glucocorticoid receptor function in patients treated with steroids for septic shock.
Intensive Care Med Exp. 3 (1): 59. -
Bergquist, M. et al. (2013) Expression of the glucocorticoid receptor is decreased in experimental Staphylococcus aureus sepsis.
J Infect. 67 (6): 574-83. -
Fragala, M.S. et al. (2011) Glucocorticoid receptor expression on human B cells in response to acute heavy resistance exercise.
Neuroimmunomodulation. 18 (3): 156-64.
View The Latest Product References
-
Hodge, G. et al. (2015) Lymphocyte senescence in COPD is associated with loss of glucocorticoid receptor expression by pro-inflammatory/cytotoxic lymphocytes.
Respir Res. 16: 2. -
Liddicoat, D.R. et al. (2014) The glucocorticoid receptor 1A3 promoter correlates with high sensitivity to glucocorticoid-induced apoptosis in human lymphocytes.
Immunol Cell Biol. 92 (10): 825-36. -
Kowalik, A. et al. (2013) Dexamethasone-FITC staining application for measurement of circadian rhythmicity of glucocorticoid receptor expression in mouse living thymocyte subsets.
J Neuroimmunol. 261 (1-2): 44-52. -
Hodge, G. et al. (2018) Bronchiolitis obliterans syndrome is associated with increased p-glycoprotein expression and loss of glucocorticoid receptor from steroid-resistant proinflammatory CD8(+) T cells.
Clin Exp Immunol. 192 (2): 242-50. -
Yu, Y. et al. (2022) Glucocorticoid receptor expression in patients with cardiac arrest in the early period after the return of spontaneous circulation: a prospective observational single-centre study
BMJ Open. 12 (9): e060246. -
Hodge, G. et al. (2022) COPD is associated with increased pro-inflammatory CD28null CD8 T and NKT-like cells in the small airways.
Clin Exp Immunol. 207 (3): 351-9.
- RRID
- AB_10844347
- UniProt
- P04150
- Entrez Gene
- NR3C1
- GO Terms
- GO:0005515 protein binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0004883 glucocorticoid receptor activity
- GO:0005496 steroid binding
- GO:0005654 nucleoplasm
- GO:0005759 mitochondrial matrix
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0008270 zinc ion binding
- GO:0016568 chromatin modification
- View More GO Terms
- GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
- GO:0043565 sequence-specific DNA binding
- GO:0051789 response to protein stimulus
- GO:0071109 superior temporal gyrus development
MCA2469
149635
150611
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