Cytokeratin 14 antibody | LL002

Mouse anti Human Cytokeratin 14

Product Type
Monoclonal Antibody
Clone
LL002
Isotype
IgG3
Specificity
Cytokeratin 14

Product Code Applications Pack Size List Price Your Price Qty
MCA890
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * IF P * WB 0.2 mg loader
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loader
MCA890T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * IF P * 20 µg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human Cytokeratin 14 antibody, clone LL002 recognizes cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14.

Mouse anti cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011)

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Elephant
Dog
Pig
Lion
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/100
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 2 1/200
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Skin

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References for Cytokeratin 14 antibody

  1. Purkis, P.E. et al. (1990) Antibody markers of basal cells in complex epithelia.
    J Cell Sci. 97 ( Pt 1): 39-50.
  2. Lane, E.B. & Alexander, C.M. (1990) Use of keratin antibodies in tumor diagnosis.
    Semin Cancer Biol. 1 (3): 165-79.
  3. Wetzels, R.H. et al. (1989) Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast.
    Am J Pathol. 134 (3): 571-9.
  4. Moll, R. et al. (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
    Cell. 31 (1): 11-24.
  5. Richardson, G.D. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells.
    J Cell Sci. 117 (Pt 16): 3539-45.
  6. Holliday, D. et al. (2009) Novel multicellular organotypic models of normal and malignant breast: tools for dissecting the role of the microenvironment in breast cancer progression.
    Breast Cancer Res. 11: R3
  7. Eastman, R. .Jr. et al. (2010) Fibroblast growth factor-10 signals development of von Brunn's nests in the exstrophic bladder.
    Am J Physiol Renal Physiol.299:F1094-110.
  8. Stumpf, P and Welsch, U. (2004) Secretory and defensive functions of the duct system of the lactating mammary gland of the African elephant (Loxodonta africana, Proboscidea)
    Zoomorphology 123:155-67
  9. View The Latest Product References
  10. Alam H et al. (2011) Loss of keratins 8 and 18 leads to alterations in α6β4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line.
    J Cell Sci. 124 (Pt 12): 2096-106.
  11. Clark, S.E. et al. (2011) Molecular subtyping of DCIS: heterogeneity of breast cancer reflected in pre-invasive disease.
    Br J Cancer. 104: 120-7.
  12. Caceres, S. et al. (2015) Establishment and Characterization of a New Cell Line of Canine Inflammatory Mammary Cancer: IPC-366.
    PLoS One. 10 (3): e0122277.
  13. Takahashi, C. et al. (2010) Newly established cell lines from mouse oral epithelium regenerate teeth when combined with dental mesenchyme.
    In Vitro Cell Dev Biol Anim. 46: 457-68.
  14. Faustino, A.M. & Dias Pereira, P. (2007) A salivary malignant myoepithelioma in a dog.
    Vet J. 173 (1): 223-6.
  15. Collins, A.T. et alet al. (2005) Prospective identification of tumorigenic prostate cancer stem cells.
    Cancer Res. 65: 10946-51.
  16. Varley, C.L. et al. (2004) Activation of peroxisome proliferator-activated receptor-gamma reverses squamous metaplasia and induces transitional differentiation in normal human urothelial cells.
    Am J Pathol. 164: 1789-98.
  17. Abdeen, S.K. et al. (2011) Wwox inactivation enhances mammary tumorigenesis.
    Oncogene. 30: 3900-6.
  18. Matos, A.J. et al. (2006) Detection of lymph node micrometastases in malignant mammary tumours in dogs by cytokeratin immunostaining.
    Vet Rec. 158: 626-30.
  19. Turner, A. et al. (2011) Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty.
    Eur Urol. 59: 447-54.
  20. Munz, B. et al. (1999) Overexpression of activin A in the skin of transgenic mice reveals new activities of activin in epidermal morphogenesis, dermal fibrosis and wound repair.
    EMBO J. 18: 5205-15.
  21. Mwase, M. et al. (2013) Cutaneous Squamous Cell Carcinoma presenting as a Wound with Discharging Sinus Tracts in a Wild African Lion (Panthera leo).
    J Comp Pathol. pii: S0021-9975(13)00106-0.
  22. Honda, K. & Tomooka, Y. (2016) Nerve-independent and ectopically additional induction of taste buds in organ culture of fetal tongues.
    In Vitro Cell Dev Biol Anim. 52 (9): 911-9.
  23. Hustler, A. et al. (2018) Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation.
    Exp Cell Res. 369 (2): 284-294.
  24. Ogihara, K. & Madarame, H. (2020) Pancreatic adenosquamous carcinoma with invasion to the spleen in a cat.
    J Vet Med Sci. 82 (9): 1395-9.
  25. Stumpf, P. & Welsch, U. (2002) Cutaneous eccrine glands of the foot pads of the rock hyrax (Procavia capensis., Hyracoidea, Mammalia.).
    Cells Tissues Organs. 171 (2-3): 215-26.
  26. Stumpf, P. et al. (2004) Cutaneous eccrine glands of the foot pads of the small Madagascan tenrec ( Echinops telfairi, Insectivora, Tenrecidae.): skin glands in a primitive mammal.
    Cell Tissue Res. 315 (1): 59-70.
  27. Sakai, Y. et al. (2019) A clonal stem cell line established from a mouse mammary placode with ability to generate functional mammary glands.
    In Vitro Cell Dev Biol Anim. 55 (10): 861-71.
  28. Yasuno, K. et al. (2013) Atypical canine mammary adenoma characterized by cystic ducts comprising a single layer of basaloid cells with myoepithelial differentiation.
    J Vet Med Sci. 75 (8): 1095-9.

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

RRID
AB_322015
UniProt
P02533
Entrez Gene
KRT14
GO Terms
GO:0005515 protein binding
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005634 nucleus
GO:0008544 epidermis development
GO:0031581 hemidesmosome assembly
GO:0045095 keratin filament
GO:0045110 intermediate filament bundle assembly
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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