Cytokeratin 14 antibody | LL002

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Mouse anti Human Cytokeratin 14:FITC

Mouse anti Human Cytokeratin 14

Mouse anti Human Cytokeratin 14

Product Type
Monoclonal Antibody
Clone
LL002
Isotype
IgG3
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA890F F* IF 0.1 mg
MCA890 C F* IF P* WB 0.2 mg
MCA890T C F* IF P* 20 µg
MCA890HT C IF P* 1 ml
Mouse anti Human Cytokeratin 14 antibody, clone LL002 recognizes cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14.

Mouse anti cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011)

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Elephant
Dog
Pig
Lion
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
0.5%Bovine Serum Albumin
Carrier Free
Yes
Immunogen
Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02533 Related reagents
Entrez Gene
KRT14 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005200 structural constituent of cytoskeleton
GO:0005634 nucleus
GO:0008544 epidermis development
GO:0031581 hemidesmosome assembly
GO:0045095 keratin filament
GO:0045110 intermediate filament bundle assembly
Regulatory
For research purposes only

Applications of Cytokeratin 14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
Immunofluorescence
Flow Cytometry 1 1/100
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 2 1/200
Western Blotting
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 Neat
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology
* This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Enzyme digestion is not recommended.
Histology Positive Control Tissue
Skin
Histology Positive Control Tissue
Skin

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG3:FITC HCA039F 0.1 mg F
Human anti Mouse IgG3:HRP HCA039P 0.1 mg E
Human anti Mouse IgG3:RPE HCA039PE 100 Tests F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F
Human anti Mouse IgG3:FITC HCA039F 0.1 mg F
Human anti Mouse IgG3:HRP HCA039P 0.1 mg E
Human anti Mouse IgG3:RPE HCA039PE 100 Tests F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Leucoperm™ BUF09 50 Tests F

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Cytokeratin 14 antibody

  1. Purkis, P.E. et al. (1990) Antibody markers of basal cells in complex epithelia.
    J Cell Sci. 97 ( Pt 1): 39-50.
  2. Lane, E.B. & Alexander, C.M. (1990) Use of keratin antibodies in tumor diagnosis.
    Semin Cancer Biol. 1 (3): 165-79.
  3. Wetzels, R.H. et al. (1989) Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast.
    Am J Pathol. 134 (3): 571-9.
  4. Moll, R. et al. (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
    Cell. 31 (1): 11-24.
  5. Richardson, G.D. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells.
    J Cell Sci. 117 (Pt 16): 3539-45.
  6. Holliday, D. et al. (2009) Novel multicellular organotypic models of normal and malignant breast: tools for dissecting the role of the microenvironment in breast cancer progression.
    Breast Cancer Res. 11: R3
  7. Eastman, R. .Jr. et al. (2010) Fibroblast growth factor-10 signals development of von Brunn's nests in the exstrophic bladder.
    Am J Physiol Renal Physiol.299:F1094-110.
  8. Stumpf, P and Welsch, U. (2004) Secretory and defensive functions of the duct system of the lactating mammary gland of the African elephant (Loxodonta africana, Proboscidea)
    Zoomorphology 123:155-67
  9. Alam H et al. (2011) Loss of keratins 8 and 18 leads to alterations in α6β4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line.
    J Cell Sci. 124 (Pt 12): 2096-106.
  10. Clark, S.E. et al. (2011) Molecular subtyping of DCIS: heterogeneity of breast cancer reflected in pre-invasive disease.
    Br J Cancer. 104: 120-7.
  11. Hale, L.P. and Markert, M.L. (2004) Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.
    J Immunol. 172: 617-24.
  12. Takahashi, C. et al. (2010) Newly established cell lines from mouse oral epithelium regenerate teeth when combined with dental mesenchyme.
    In Vitro Cell Dev Biol Anim. 46: 457-68.
  13. Faustino, A.M. et al. (2007) A salivary malignant myoepithelioma in a dog.
    Faustino, A.M. and Dias Pereira, P.
  14. Collins, A.T. et alet al. (2005) Prospective identification of tumorigenic prostate cancer stem cells.
    Cancer Res. 65: 10946-51.
  15. Varley, C.L. et al. (2004) Activation of peroxisome proliferator-activated receptor-gamma reverses squamous metaplasia and induces transitional differentiation in normal human urothelial cells.
    Am J Pathol. 164: 1789-98.
  16. Abdeen, S.K. et al. (2011) Wwox inactivation enhances mammary tumorigenesis.
    Oncogene. 30: 3900-6.
  17. Matos, A.J. et al. (2006) Detection of lymph node micrometastases in malignant mammary tumours in dogs by cytokeratin immunostaining.
    Vet Rec. 158: 626-30.
  18. Turner, A. et al. (2011) Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty.
    Eur Urol. 59: 447-54.
  19. Munz, B. et al. (1999) Overexpression of activin A in the skin of transgenic mice reveals new activities of activin in epidermal morphogenesis, dermal fibrosis and wound repair.
    EMBO J. 18: 5205-15.
  20. Mwase, M. et al. (2013) Cutaneous Squamous Cell Carcinoma presenting as a Wound with Discharging Sinus Tracts in a Wild African Lion (Panthera leo).
    J Comp Pathol. pii: S0021-9975(13)00106-0.
  21. Caceres, S. et al. (2015) Establishment and Characterization of a New Cell Line of Canine Inflammatory Mammary Cancer: IPC-366.
    PLoS One. 10 (3): e0122277.
  22. Honda, K. & Tomooka, Y. (2016) Nerve-independent and ectopically additional induction of taste buds in organ culture of fetal tongues.
    <em>In Vitro</em> Cell Dev Biol Anim. 52 (9): 911-9.