Cytokeratin 14 antibody | LL002
Mouse anti Human cytokeratin 14 antibody, clone LL002 (MCA890) used for the detection of CK14 expressing cells in a canine inflammatory mammary cancer pellet section by immunohistochemistry.
Image caption:
Immunohistochemistry of IPC-366 pellet, paraffin sections. A) Pancytokeratin (AE1/AE3) (10X); intense immunolabeling and numerous positive cells. B) CK14; intense immunolabeling and numerous positive cells (10X). C) Vimentin; moderate immunolabeling and numerous positive cells (10X). D) p63; negative (20X). E) α-smooth muscle actin, negative cells (10X). F) Estrogen receptor; negative cells (20X). G) Progesterone receptor; negative cells (20X). H) HER-2; negative cells (20X). I) E-Cadherin; intense membranous immunolabeling in numerous cells (20X). J) COX-2; most of the cells are moderately positive; some cells are intensely positive (10X). K) COX-2; some cells strongly positive showing features of malignancy (binucleated cell, arrow) (40X). L) Ki-67 proliferation marker; many nuclei are positive (10X).
From: Caceres S, Peña L, de Andres PJ, Illera MJ, Lopez MS, et al. (2015)
Establishment and Characterization of a New Cell Line of Canine Inflammatory Mammary Cancer: IPC-366.
PLoS ONE 10(3): e0122277.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti human Cytokeratin 14 antibody, clone LL002 (MCA890) used for the demonstration of cytokeratin 14 expressing cells in human buccal epithelium and urothelium by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Comparison between buccal epithelium and urothelium. (A-B) Histological sections showing representative in situ immunolocalisation patterns for cytokeratins CK5, CK7, CK13, CK14 and CK20 in (A) buccal mucosa (scale bar ≡ 100 μm) and (B) urothelium (scale bar ≡ 25 μm).
From: Hustler, A. et al. 2018
Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation
Exp Cell Res. 369(2):284-94.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti human Cytokeratin 14 antibody, clone LL002 (MCA890) used for the demonstration of cytokeratin 14 expressing cells in human buccal epithelium and urothelium by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Formation of cell sheets and barrier function. The ability to form a stratified barrier epithelium was examined in three independent NHB cell lines, with a representative NHU cell line provided for comparison purposes. Immunohistochemical analysis of NHB and NHU cell sheets for CK13 and CK14 protein expression. Representative results shown from experiments performed on n = 3 independent NHB cell lines. Scale bar ≡ 50 μm
From: Hustler, A. et al. 2018
Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation
Exp Cell Res. 369(2):284-94.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
P WB ResetMouse anti Human Cytokeratin 14
- Product Type
- Monoclonal Antibody
- Clone
- LL002
- Isotype
- IgG3
- Specificity
- Cytokeratin 14
Antibody Quality and Performance Guarantee
| Mouse anti Human Cytokeratin 14 antibody, clone LL002 recognizes cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Mouse anti cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011) |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Elephant Dog Pig Lion - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/100 | |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 2 | |
1/200 | |
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Skin
References for Cytokeratin 14 antibody
-
Purkis, P.E. et al. (1990) Antibody markers of basal cells in complex epithelia.
J Cell Sci. 97 ( Pt 1): 39-50. -
Lane, E.B. & Alexander, C.M. (1990) Use of keratin antibodies in tumor diagnosis.
Semin Cancer Biol. 1 (3): 165-79. -
Wetzels, R.H. et al. (1989) Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast.
Am J Pathol. 134 (3): 571-9. -
Moll, R. et al. (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
Cell. 31 (1): 11-24. -
Richardson, G.D. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells.
J Cell Sci. 117 (Pt 16): 3539-45. -
Holliday, D. et al. (2009) Novel multicellular organotypic models of normal and malignant breast: tools for dissecting the role of the microenvironment in breast cancer progression.
Breast Cancer Res. 11: R3 -
Eastman, R. .Jr. et al. (2010) Fibroblast growth factor-10 signals development of von Brunn's nests in the exstrophic bladder.
Am J Physiol Renal Physiol.299:F1094-110. -
Stumpf, P and Welsch, U. (2004) Secretory and defensive functions of the duct system of the lactating mammary gland of the African elephant (Loxodonta africana, Proboscidea)
Zoomorphology 123:155-67
View The Latest Product References
-
Alam H et al. (2011) Loss of keratins 8 and 18 leads to alterations in α6β4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line.
J Cell Sci. 124 (Pt 12): 2096-106. -
Clark, S.E. et al. (2011) Molecular subtyping of DCIS: heterogeneity of breast cancer reflected in pre-invasive disease.
Br J Cancer. 104: 120-7. -
Caceres, S. et al. (2015) Establishment and Characterization of a New Cell Line of Canine Inflammatory Mammary Cancer: IPC-366.
PLoS One. 10 (3): e0122277. -
Takahashi, C. et al. (2010) Newly established cell lines from mouse oral epithelium regenerate teeth when combined with dental mesenchyme.
In Vitro Cell Dev Biol Anim. 46: 457-68. -
Faustino, A.M. & Dias Pereira, P. (2007) A salivary malignant myoepithelioma in a dog.
Vet J. 173 (1): 223-6. -
Collins, A.T. et alet al. (2005) Prospective identification of tumorigenic prostate cancer stem cells.
Cancer Res. 65: 10946-51. -
Varley, C.L. et al. (2004) Activation of peroxisome proliferator-activated receptor-gamma reverses squamous metaplasia and induces transitional differentiation in normal human urothelial cells.
Am J Pathol. 164: 1789-98. -
Abdeen, S.K. et al. (2011) Wwox inactivation enhances mammary tumorigenesis.
Oncogene. 30: 3900-6. -
Matos, A.J. et al. (2006) Detection of lymph node micrometastases in malignant mammary tumours in dogs by cytokeratin immunostaining.
Vet Rec. 158: 626-30. -
Turner, A. et al. (2011) Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty.
Eur Urol. 59: 447-54. -
Munz, B. et al. (1999) Overexpression of activin A in the skin of transgenic mice reveals new activities of activin in epidermal morphogenesis, dermal fibrosis and wound repair.
EMBO J. 18: 5205-15. -
Mwase, M. et al. (2013) Cutaneous Squamous Cell Carcinoma presenting as a Wound with Discharging Sinus Tracts in a Wild African Lion (Panthera leo).
J Comp Pathol. pii: S0021-9975(13)00106-0. -
Honda, K. & Tomooka, Y. (2016) Nerve-independent and ectopically additional induction of taste buds in organ culture of fetal tongues.
In Vitro Cell Dev Biol Anim. 52 (9): 911-9. -
Hustler, A. et al. (2018) Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation.
Exp Cell Res. 369 (2): 284-294. -
Ogihara, K. & Madarame, H. (2020) Pancreatic adenosquamous carcinoma with invasion to the spleen in a cat.
J Vet Med Sci. 82 (9): 1395-9. -
Stumpf, P. & Welsch, U. (2002) Cutaneous eccrine glands of the foot pads of the rock hyrax (Procavia capensis., Hyracoidea, Mammalia.).
Cells Tissues Organs. 171 (2-3): 215-26. -
Stumpf, P. et al. (2004) Cutaneous eccrine glands of the foot pads of the small Madagascan tenrec ( Echinops telfairi, Insectivora, Tenrecidae.): skin glands in a primitive mammal.
Cell Tissue Res. 315 (1): 59-70. -
Sakai, Y. et al. (2019) A clonal stem cell line established from a mouse mammary placode with ability to generate functional mammary glands.
In Vitro Cell Dev Biol Anim. 55 (10): 861-71. -
Yasuno, K. et al. (2013) Atypical canine mammary adenoma characterized by cystic ducts comprising a single layer of basaloid cells with myoepithelial differentiation.
J Vet Med Sci. 75 (8): 1095-9.
- RRID
- AB_322015
- UniProt
- P02533
- Entrez Gene
- KRT14
- GO Terms
- GO:0005515 protein binding
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0005200 structural constituent of cytoskeleton
- GO:0005634 nucleus
- GO:0008544 epidermis development
- GO:0031581 hemidesmosome assembly
- GO:0045095 keratin filament
- GO:0045110 intermediate filament bundle assembly
- View More GO Terms
View more products with CYTOKERATIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up