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CD83 antibody | HB15e

Product Code Applications Pack Size List Price Your Price Qty
MCA1582T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF P * 20 µg loader
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loader
MCA1582GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP P * 0.1 mg loader
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loader
MCA1582
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP P * 0.2 mg loader
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loader

Mouse anti Human CD83 antibody, clone HB15e recognizes the human CD83 cell surface antigen, a 40-45 kDa glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA.

In immunohistology CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein (Zhou et al. 1992).

However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage (Zhou et al. 1995).CD83+ cells co-express the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules (Zhou et al.1995) and Zhou and Tedder 1995).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Chimpanzee
Baboon
Rhesus Monkey
Tasmanian Devil
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA1582T, MCA1582GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA1582: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Cos cells transfected with HB15 cDNA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1/500 1/1000
Immunohistology - Paraffin 1 1/50 1/50 Pack Size: 20 µg
1/100 Pack Size: 0.1 mg, 0.2 mg
Immunoprecipitation
  1. 1 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106cells in 100μl
Histology Positive Control Tissue
Human tonsil

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References for CD83 antibody

  1. Zhou, L.J. et al. (1992) A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily.
    J Immunol. 149 (2): 735-42.
  2. Zhou, L.J. & Tedder, T.F. (1995) Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily.
    J Immunol. 154 (8): 3821-35.
  3. Schlossman, S.F., et al. Eds. Engel, P. et al. (1995) 'CD83 Workshop report' in Leucocyte Typing V, White Cell Differentiation Antigens,
    Oxford University Press pp. 693-5.
  4. Hesselink, D.A. et al. (2005) The effects of renal transplantation on circulating dendritic cells.
    Clin Exp Immunol. 140: 384-93.
  5. Walker, J.G. et al. (2007) Characterisation of a dendritic cell subset in synovial tissue which strongly expresses Jak/STAT transcription factors from patients with rheumatoid arthritis.
    Ann Rheum Dis. 66: 992-9.
  6. Ifergan, I. et al. (2008) The blood-brain barrier induces differentiation of migrating monocytes into Th17-polarizing dendritic cells.
    Brain. 131: 785-99.
  7. Denniston, A.K. et al. (2011) Endogenous Cortisol and TGF-{beta} in Human Aqueous Humor Contribute to Ocular Immune Privilege by Regulating Dendritic Cell Function.
    J Immunol. 186:305-11.
  8. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. ; 12:2.
  9. View The Latest Product References
  10. Van Vré, E.A. et al. (2011) Immunohistochemical characterisation of dendritic cells in human atherosclerotic lesions: possible pitfalls.
    Pathology. 43 (3): 239-47.
  11. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
    J Biomed Biotechnol. 2012: 172420.
  12. Sprater, F. et al. (2012) Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells
    PLoS One. 7: e49577.
  13. Shikotra, A. et al. (2012) Increased expression of immunoreactive thymic stromal lymphopoietin in patients with severe asthma.
    J Allergy Clin Immunol. 129: 104-11.e1-9.
  14. Denniston, A.K. et al. (2012) Aqueous humor suppression of dendritic cell function helps maintain immune regulation in the eye during human uveitis.
    Invest Ophthalmol Vis Sci. 53 (2): 888-96.
  15. Howson, L.J. et al. (2014) Identification of dendritic cells, B cell and T cell subsets in Tasmanian devil lymphoid tissue; evidence for poor immune cell infiltration into devil facial tumors.
    Anat Rec (Hoboken). 297: 925-38.
  16. Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
    Microsc Res Tech. 79 (7): 604-14.
  17. Wang, P. et al. (2016) Distribution and expression profiles of dendritic cell subpopulations in human bladder cancer.
    Int J Clin Exp Pathol 9(7):7180-7.
  18. Duan, Y.G. et al. (2016) Characterisation of dendritic cell subsets in chronically inflamed human epididymis.
    Andrologia. 48 (4): 431-40.
  19. Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
    Parasit Vectors. 11 (1): 640.
  20. Arya, S. et al. (2019) Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study.
    Sci Rep. 9 (1): 4343.
  21. Yildiz, M. et al. (2019) Histological and immunohistochemical studies of the proximal caecum and caecal tonsils of quail (Coturnix coturnix japonica).
    Anat Histol Embryol. 48 (5): 476-85.
  22. Eren, U. et al. (2022) TLR2 and TLR4 molecules and antigen-presenting cell compositions in cecal tonsils of broiler chicks (Gallus gallus domesticus.) in the first two weeks of the post-hatch period.
    Anat Histol Embryol. 51 (1): 125-35.
  23. Barbieri, S. et al. (2022) Does smoking habit affect dendritic cell expression in oral squamous cell carcinoma?
    Braz Oral Res. 36: e044.

Synonyms
HB15
RRID
AB_321773
UniProt
Q01151
Entrez Gene
CD83
GO Terms
GO:0006952 defense response
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0006959 humoral immune response

MCA1582T

164014 1802

MCA1582GA

0715 158899

MCA1582

149726

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