CD83 antibody | HB15e
Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1α,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-α, IL-1β, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.
From: Sprater F, Hovden A-O, Appel S (2012)
Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.
PLoS ONE 7(11): e49577.
Mouse anti Human CD83:FITC
Mouse anti Human CD83
Mouse anti Human CD83:RPE
- Product Type
- Monoclonal Antibody
- Clone
- HB15e
- Isotype
- IgG1
Mouse anti Human CD83 antibody, clone HB15e recognizes the human CD83 cell surface antigen, a 40-45 kDa glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA.
In immunohistology CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein (Zhou et al. 1992).
However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage (Zhou et al. 1995).CD83+ cells co-express the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules (Zhou et al.1995) and Zhou and Tedder 1995).
In immunohistology CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein (Zhou et al. 1992).
However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage (Zhou et al. 1995).CD83+ cells co-express the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules (Zhou et al.1995) and Zhou and Tedder 1995).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Chimpanzee Baboon Rhesus Monkey Tasmanian Devil - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 25 TestsReconstitute in 0.25 ml disilled waterPack Size: 100 TestsReconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Cos cells transfected with HB15 cDNA.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- Q01151 Related reagents
- Entrez Gene
- CD83 Related reagents
- GO Terms
- GO:0006952 defense response
- GO:0007165 signal transduction
- GO:0005887 integral to plasma membrane
- GO:0006959 humoral immune response
- Regulatory
- For research purposes only
Applications of CD83 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/20 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/500 | 1/1000 | |
| Immunohistology - Paraffin 1 | 1/50 | 1/50 Pack Size: 20 µg 1/100 Pack Size: 0.1 mg, 0.2 mg |
|
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/5 |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106cells in 100ul.
- Histology Positive Control Tissue
- Human Tonsil
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | ||
| Mouse IgG1 Negative Control:RPE | MCA928PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD83 antibody
-
Zhou, L.J. et al. (1992) A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily.
J Immunol. 149 (2): 735-42. -
Zhou, L.J. & Tedder, T.F. (1995) Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily.
J Immunol. 154 (8): 3821-35. -
Zhou, L.J. & Tedder, T.F. (1995) A distinct pattern of cytokine gene expression by human CD83+ blood dendritic cells.
Blood. 86 (9): 3295-301. -
Denniston, A.K. et al. (2011) Endogenous Cortisol and TGF-{beta} in Human Aqueous Humor Contribute to Ocular Immune Privilege by Regulating Dendritic Cell Function.
J Immunol. 186:305-11. -
Schlossman, S.F., et al. Eds. Engel, P. et al. (1995) 'CD83 Workshop report' in Leucocyte Typing V, White Cell Differentiation Antigens,
Oxford University Press pp. 693-5. -
Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
Exp Anim. 49 (2): 97-110. -
Hesselink, D.A. et al. (2005) The effects of renal transplantation on circulating dendritic cells.
Clin Exp Immunol. 140: 384-93. -
Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
BMC Immunol. ; 12:2. -
Ifergan, I. et al. (2008) The blood-brain barrier induces differentiation of migrating monocytes into Th17-polarizing dendritic cells.
Brain. 131: 785-99. -
Walker, J.G. et al. (2007) Characterisation of a dendritic cell subset in synovial tissue which strongly expresses Jak/STAT transcription factors from patients with rheumatoid arthritis.
Ann Rheum Dis. 66: 992-9. -
Köller, M. et al. (2004) Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients.
Int Immunol. 16: 1595-604. -
Denniston, A.K. et al. (2012) Aqueous humor suppression of dendritic cell function helps maintain immune regulation in the eye during human uveitis.
Invest Ophthalmol Vis Sci. 53 (2): 888-96. -
Shikotra, A. et al. (2012) Increased expression of immunoreactive thymic stromal lymphopoietin in patients with severe asthma.
J Allergy Clin Immunol. 129: 104-11.e1-9. -
Sprater, F. et al. (2012) Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells
PLoS One. 7: e49577. -
Howson, L.J. et al. (2014) Identification of dendritic cells, B cell and T cell subsets in Tasmanian devil lymphoid tissue; evidence for poor immune cell infiltration into devil facial tumors.
Anat Rec (Hoboken). 297: 925-38. -
Eren U et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
Microsc Res Tech. Apr 26. [Epub ahead of print] -
Wang, P. et al. (2016) Distribution and expression profiles of dendritic cell subpopulations in human bladder cancer.
Int J Clin Exp Pathol 9(7):7180-7. -
Van Vré, E.A. et al. (2011) Immunohistochemical characterisation of dendritic cells in human atherosclerotic lesions: possible pitfalls.
Pathology. 43 (3): 239-47. -
Duan, Y.G. et al. (2016) Characterisation of dendritic cell subsets in chronically inflamed human epididymis.
Andrologia. 48 (4): 431-40.
