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CD83 antibody | HB15e

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Anti Human CD83 Antibody, clone HB15e thumbnail image 1 Anti Human CD83 Antibody, clone HB15e thumbnail image 2 Anti Human CD83 Antibody, clone HB15e thumbnail image 3 Anti Human CD83 Antibody, clone HB15e thumbnail image 4 Anti Human CD83 Antibody, clone HB15e thumbnail image 5 Anti Human CD83 Antibody, clone HB15e thumbnail image 6 Anti Human CD83 Antibody, clone HB15e thumbnail image 7 Anti Human CD83 Antibody, clone HB15e thumbnail image 8 Anti Human CD83 Antibody, clone HB15e thumbnail image 9 Anti Human CD83 Antibody, clone HB15e thumbnail image 10 Anti Human CD83 Antibody, clone HB15e thumbnail image 11 Anti Human CD83 Antibody, clone HB15e thumbnail image 12
Anti Human CD83 Antibody, clone HB15e gallery image 1
Staining of KM-H2 cell line with Mouse anti Human CD83:FITC (MCA1582F)
Anti Human CD83 Antibody, clone HB15e gallery image 2
Staining of KM-H2 cells with Mouse anti Human CD83 (MCA1582)
Anti Human CD83 Antibody, clone HB15e gallery image 3
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582) followed by the Histar detection system (STAR 3000). Low power
Anti Human CD83 Antibody, clone HB15e gallery image 4
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582) followed by the Histar detection system (STAR 3000). Low power
Anti Human CD83 Antibody, clone HB15e gallery image 5
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582) followed by the Histar detection system (STAR 3000). Medium power
Anti Human CD83 Antibody, clone HB15e gallery image 6
Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582) followed by the Histar detection system (STAR 3000). High power
Anti Human CD83 Antibody, clone HB15e gallery image 7
Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582), red in A and Mouse anti Human CD21 antibody, clone LB21 (MCA1195), green in B. The merged image is in C with nuclei counterstained blue using DAPI. Low power
Anti Human CD83 Antibody, clone HB15e gallery image 8
Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582), red in A and Mouse anti Human CD21 antibody, clone LB21 (MCA1195), green in B. The merged image is in C with nuclei counterstained blue using DAPI. High power
Anti Human CD83 Antibody, clone HB15e gallery image 9
Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e (MCA1582PE) used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.
Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1α,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-α, IL-1β, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.

From: Sprater F, Hovden A-O, Appel S (2012)
Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.
PLoS ONE 7(11): e49577.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD83 Antibody, clone HB15e gallery image 10
Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e (MCA1582), red in A and Mouse anti Human CD21 antibody, clone LB21 (MCA1195), green in B. The merged image is in C with nuclei counterstained blue using DAPI. Medium power
Anti Human CD83 Antibody, clone HB15e gallery image 11
Published customer image:
FITC conjugated Mouse anti Human CD83 antibody, clone HB15e (MCA1582F) used for the evaluation of CD83 expression on ovine peritoneal cells by flow cytometry.
Image caption:
Percentage of cell populations in the peritoneal fluid during the early stages of infection. The specific cell populations are shown as follows: (a) TCD4; (b) TCD8; (c) WC1+γδ; (d) CD14; (e) MHCII; (f) CD83. Cell subsets of uninfected (Neg. Ctl) and infected sheep (Group 1) are shown in columns. Mann-Whitney U-test was used to compare data from Groups 1 and 3. Values represent the mean ± standard deviation (SD). Asterisks indicate different levels of significance between groups: *P ≤ 0.05, **P ≤ 0.01

From: Pérez-Caballero R, Javier Martínez-Moreno F, Zafra R, Molina-Hernández V, Pacheco IL, Teresa Ruiz-Campillo M, Escamilla A, Pérez J, Martnez-Moreno Á, Buffoni L.
Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 2018 11(1):640.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD83 Antibody, clone HB15e gallery image 12
Published customer image:
Mouse anti Human CD83 antibody, clone HB15e (MCA1582) used for the evaluation of CD83 expression on cell populations by flow cytometry.
Image caption:
DC differentiation from H1 hESC.
Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.

From: Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ.
Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012;2012:172420.
doi: 10.1155/2012/172420.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD83:FITC

Mouse anti Human CD83

Product Type
Monoclonal Antibody
Clone
HB15e
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1582F
Datasheet
F
SDS
0.1 mg loader

MCA1582T
Datasheet
C F IF P*
SDS
20 µg loader

MCA1582GA
Datasheet
C F IF IP P*
SDS
0.1 mg loader

MCA1582
Datasheet
C F IF IP P*
SDS
0.2 mg loader

Mouse anti Human CD83 antibody, clone HB15e recognizes the human CD83 cell surface antigen, a 40-45 kDa glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA.

In immunohistology CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein (Zhou et al. 1992).

However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage (Zhou et al. 1995).CD83+ cells co-express the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules (Zhou et al.1995) and Zhou and Tedder 1995).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Chimpanzee
Baboon
Rhesus Monkey
Tasmanian Devil
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Cos cells transfected with HB15 cDNA.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q01151
Entrez Gene
CD83
GO Terms
GO:0006952 defense response
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0006959 humoral immune response
Regulatory
For research purposes only

Applications of CD83 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1/500 1/1000
Immunohistology - Paraffin 1 1/50 1/50 Pack Size: 20 µg
1/100 Pack Size: 0.1 mg, 0.2 mg
Immunoprecipitation
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
Histology Positive Control Tissue
Human Tonsil
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD83 antibody

  1. Zhou, L.J. et al. (1992) A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily.
    J Immunol. 149 (2): 735-42.
  2. Zhou, L.J. & Tedder, T.F. (1995) Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily.
    J Immunol. 154 (8): 3821-35.
  3. Zhou, L.J. & Tedder, T.F. (1995) A distinct pattern of cytokine gene expression by human CD83+ blood dendritic cells.
    Blood. 86 (9): 3295-301.
  4. Denniston, A.K. et al. (2011) Endogenous Cortisol and TGF-{beta} in Human Aqueous Humor Contribute to Ocular Immune Privilege by Regulating Dendritic Cell Function.
    J Immunol. 186:305-11.
  5. Schlossman, S.F., et al. Eds. Engel, P. et al. (1995) 'CD83 Workshop report' in Leucocyte Typing V, White Cell Differentiation Antigens,
    Oxford University Press pp. 693-5.
  6. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  7. Hesselink, D.A. et al. (2005) The effects of renal transplantation on circulating dendritic cells.
    Clin Exp Immunol. 140: 384-93.
  8. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. ; 12:2.
  9. Ifergan, I. et al. (2008) The blood-brain barrier induces differentiation of migrating monocytes into Th17-polarizing dendritic cells.
    Brain. 131: 785-99.
  10. Walker, J.G. et al. (2007) Characterisation of a dendritic cell subset in synovial tissue which strongly expresses Jak/STAT transcription factors from patients with rheumatoid arthritis.
    Ann Rheum Dis. 66: 992-9.
  11. Köller, M. et al. (2004) Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients.
    Int Immunol. 16: 1595-604.
  12. Denniston, A.K. et al. (2012) Aqueous humor suppression of dendritic cell function helps maintain immune regulation in the eye during human uveitis.
    Invest Ophthalmol Vis Sci. 53 (2): 888-96.
  13. Shikotra, A. et al. (2012) Increased expression of immunoreactive thymic stromal lymphopoietin in patients with severe asthma.
    J Allergy Clin Immunol. 129: 104-11.e1-9.
  14. Sprater, F. et al. (2012) Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells
    PLoS One. 7: e49577.
  15. Howson, L.J. et al. (2014) Identification of dendritic cells, B cell and T cell subsets in Tasmanian devil lymphoid tissue; evidence for poor immune cell infiltration into devil facial tumors.
    Anat Rec (Hoboken). 297: 925-38.
  16. Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
    Microsc Res Tech. 79 (7): 604-14.
  17. Wang, P. et al. (2016) Distribution and expression profiles of dendritic cell subpopulations in human bladder cancer.
    Int J Clin Exp Pathol 9(7):7180-7.
  18. Van Vré, E.A. et al. (2011) Immunohistochemical characterisation of dendritic cells in human atherosclerotic lesions: possible pitfalls.
    Pathology. 43 (3): 239-47.
  19. Duan, Y.G. et al. (2016) Characterisation of dendritic cell subsets in chronically inflamed human epididymis.
    Andrologia. 48 (4): 431-40.
  20. Arya, S. et al. (2019) Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study.
    Sci Rep. 9 (1): 4343.
  21. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
    J Biomed Biotechnol. 2012: 172420.
  22. Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
    Parasit Vectors. 11 (1): 640.
  23. Yildiz, M. et al. (2019) Histological and immunohistochemical studies of the proximal caecum and caecal tonsils of quail (Coturnix coturnix japonica).
    Anat Histol Embryol. 48 (5): 476-85.

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