CD83 antibody | HB15e

100% Secure


Mouse anti Human CD83:FITC

Mouse anti Human CD83

Mouse anti Human CD83:RPE

Product Type
Monoclonal Antibody
Clone
HB15e
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1582F F 0.1 mg
MCA1582 C F IF IP P* 0.2 mg
MCA1582GA C F IF IP P* 0.1 mg
MCA1582T C F IF P* 20 µg
MCA1582PE F 100 Tests
MCA1582PET F 25 Tests
Mouse anti Human CD83 antibody, clone HB15e recognizes the human CD83 cell surface antigen, a 40-45 kDa glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA.

In immunohistology CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan's cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein (Zhou et al. 1992).

However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage (Zhou et al. 1995).CD83+ cells co-express the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules (Zhou et al.1995) and Zhou and Tedder 1995).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
Chimpanzee
Baboon
Rhesus Monkey
Tasmanian Devil
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 100 Tests
Reconstitute with 1.0 ml distilled water
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Cos cells transfected with HB15 cDNA.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
Q01151 Related reagents
Entrez Gene
CD83 Related reagents
GO Terms
GO:0006952 defense response
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0006959 humoral immune response
Regulatory
For research purposes only

Applications of CD83 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/20
Flow Cytometry 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1/500 1/1000
Immunohistology - Paraffin 1 1/50 1/100 Pack Size: 0.2 mg, 0.1 mg
1/50 Pack Size: 20 µg
Immunoprecipitation
Flow Cytometry Neat 1/5
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
Histology Positive Control Tissue
Human Tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD83 antibody

  1. Zhou, L.J. et al. (1992) A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily.
    J Immunol. 149 (2): 735-42.
  2. Zhou, L.J. & Tedder, T.F. (1995) Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily.
    J Immunol. 154 (8): 3821-35.
  3. Zhou, L.J. & Tedder, T.F. (1995) A distinct pattern of cytokine gene expression by human CD83+ blood dendritic cells.
    Blood. 86 (9): 3295-301.
  4. Denniston, A.K. et al. (2011) Endogenous Cortisol and TGF-{beta} in Human Aqueous Humor Contribute to Ocular Immune Privilege by Regulating Dendritic Cell Function.
    J Immunol. 186:305-11.
  5. Schlossman, S.F., et al. Eds. Engel, P. et al. (1995) 'CD83 Workshop report' in Leucocyte Typing V, White Cell Differentiation Antigens,
    Oxford University Press pp. 693-5.
  6. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  7. Hesselink, D.A. et al. (2005) The effects of renal transplantation on circulating dendritic cells.
    Clin Exp Immunol. 140: 384-93.
  8. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. ; 12:2.
  9. Ifergan, I. et al. (2008) The blood-brain barrier induces differentiation of migrating monocytes into Th17-polarizing dendritic cells.
    Brain. 131: 785-99.
  10. Walker, J.G. et al. (2007) Characterisation of a dendritic cell subset in synovial tissue which strongly expresses Jak/STAT transcription factors from patients with rheumatoid arthritis.
    Ann Rheum Dis. 66: 992-9.
  11. Köller, M. et al. (2004) Phenotypic and functional deficiencies of monocyte-derived dendritic cells in systemic lupus erythematosus (SLE) patients.
    Int Immunol. 16: 1595-604.
  12. Denniston, A.K. et al. (2012) Aqueous humor suppression of dendritic cell function helps maintain immune regulation in the eye during human uveitis.
    Invest Ophthalmol Vis Sci. 53 (2): 888-96.
  13. Shikotra, A. et al. (2012) Increased expression of immunoreactive thymic stromal lymphopoietin in patients with severe asthma.
    J Allergy Clin Immunol. 129: 104-11.e1-9.
  14. Sprater, F. et al. (2012) Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells
    PLoS One. 7: e49577.
  15. Howson, L.J. et al. (2014) Identification of dendritic cells, B cell and T cell subsets in Tasmanian devil lymphoid tissue; evidence for poor immune cell infiltration into devil facial tumors.
    Anat Rec (Hoboken). 297: 925-38.
  16. Eren U et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
    Microsc Res Tech. Apr 26. [Epub ahead of print]
  17. Wang, P. et al. (2016) Distribution and expression profiles of dendritic cell subpopulations in human bladder cancer.
    Int J Clin Exp Pathol 9(7):7180-7.
  18. Van Vré, E.A. et al. (2011) Immunohistochemical characterisation of dendritic cells in human atherosclerotic lesions: possible pitfalls.
    Pathology. 43 (3): 239-47.
  19. Duan, Y.G. et al. (2016) Characterisation of dendritic cell subsets in chronically inflamed human epididymis.
    Andrologia. 48 (4): 431-40.