CD79a antibody | HM57
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F P ResetMouse anti Human CD79a
- Product Type
- Monoclonal Antibody
- Clone
- HM57
- Isotype
- IgG1
- Specificity
- CD79a
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| Mouse anti Human CD79a antibody, clone HM57 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45 kDa protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signaling following antigen-binding to surface immunoglobulin. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit Horse Pig Monkey Rat Bovine Guinea Pig Fallow deer American Bison Red deer Ferret Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 0.1 mg, 25 µg, 0.2 mg
0.09% Sodium Azide (NaN3) Pack Size: 1 ml0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Carrier Free
- Yes
- Immunogen
- Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1
- Approx. Protein Concentrations
- Pack Size: 0.1 mg, 25 µg, 0.2 mgIgG concentration 1.0mg/mlPack Size: 1 mlIgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/50 Pack Size: 0.1 mg, 0.2 mg 1/10 Pack Size: 1 ml, 25 µg |
1/100 Pack Size: 0.1 mg, 0.2 mg 1/25 Pack Size: 1 ml 1/50 Pack Size: 25 µg |
| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 2 | |
1/100 | 1/500 |
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Human tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Mason, D.Y. et al. (1991) The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
J Immunol. 147 (11): 2474-82.
References for CD79a antibody
-
Jones, M. et al. (1993) Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
J Immunol. 150 (12): 5429-35. -
Nelson, D.D. et al. (2010) CD8(+)/perforin(+)/WC1(-) gammadelta T cells, not CD8(+) alphabeta T cells, infiltrate vasculitis lesions of American bison (Bison bison) with experimental sheep-associated malignant catarrhal fever.
Vet Immunol Immunopathol. 136: 284-91. -
De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
Cytometry A. 81 (4): 312-23. -
Spaas, J.H. et al. (2013) Culture and characterisation of equine peripheral blood mesenchymal stromal cells.
Vet J. 195 (1): 107-13. -
Moore, P.F. et al. (2013) Canine inflamed nonepitheliotropic cutaneous T-cell lymphoma: a diagnostic conundrum.
Vet Dermatol. 24 (1): 204-11.e44-5. -
De Schauwer, C. et al. (2014) Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources.
Stem Cell Res Ther. 5 (1): 6. -
Bozkurt, Y.A., et al. (2014) Histological and immunohistological studies of the structure of lymph nodes in Kilis goats.
Biotech Histochem. 89(6):440-5. -
Paebst, F. et al. (2014) Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: an approach toward a standardized definition.
Cytometry A. 85 (8): 678-87.
View The Latest Product References
-
Gelain ME et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
Vet Immunol Immunopathol. 159 (1-2): 91-6. -
Claessen, C. et al. (2015) Equid herpesvirus 1 (EHV1) infection of equine mesenchymal stem cells induces a pUL56-dependent downregulation of select cell surface markers.
Vet Microbiol. 176 (1-2): 32-9. -
Aresu, L. et al. (2015) Canine indolent and aggressive lymphoma: clinical spectrum with histologic correlation.
Vet Comp Oncol. 13 (4): 348-62. -
Poggi, A. et al. (2015) Flow cytometric evaluation of ki67 for the determination of malignancy grade in canine lymphoma.
Vet Comp Oncol. 13 (4): 475-80. -
Froment, R. & Bédard, C. (2016) Marked hyperphosphatasemia associated with an acute leukemia in a Great Dane.
Vet Clin Pathol. 45 (3): 459-65. -
Schinköthe J >et al. (2016) Characterization of tuberculous granulomas in different stages of progression and associated tertiary lymphoid tissue in goats experimentally infected with Mycobacterium avium subsp. hominissuis.
Comp Immunol Microbiol Infect Dis. 47: 41-51. -
Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
Vet Comp Oncol. 14 (4): 409-16. -
Hillmann, A. et al. (2016) Comparative Characterization of Human and Equine Mesenchymal Stromal Cells: A Basis for Translational Studies in the Equine Model.
Cell Transplant. 25 (1): 109-24. -
Long, H. et al. (2016) Polyostotic Lymphoma in a Ferret (Mustela putorius furo).
J Comp Pathol. 154 (4): 341-4. -
Wessels, M. et al. (2017) Systemic necrotizing polyarteritis in three weaned lambs from one flock.
J Vet Diagn Invest 29 (5) :733-37. -
Uitterdijk, A. et al. (2017) Time course of VCAM-1 expression in reperfused myocardial infarction in swine and its relation to retention of intracoronary administered bone marrow-derived mononuclear cells.
PLoS One. 12 (6): e0178779. -
Nagata, K. et al. (2017) Epstein-Barr Virus Lytic Reactivation Activates B Cells Polyclonally and Induces Activation-Induced Cytidine Deaminase Expression: A Mechanism Underlying Autoimmunity and Its Contribution to Graves' Disease.
Viral Immunol. 30 (3): 240-9. -
Collins, J.J.P. et al. (2018) Impaired Angiogenic Supportive Capacity and Altered Gene Expression Profile of Resident CD146+ Mesenchymal Stromal Cells Isolated from Hyperoxia-Injured Neonatal Rat Lungs.
Stem Cells Dev. 27 (16): 1109-24. -
Murphy, E.G. et al. (2019) First detection of Hepatitis E virus (Orthohepevirus C) in wild brown rats (Rattus norvegicus.) from Great Britain.
Zoonoses Public Health. 66 (6): 686-94. -
Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
PLoS One. 14 (10): e0223647. -
Mu&mtilde;oz-Silvestre, A. et al. (2020) Pathogenesis of Intradermal Staphylococcal Infections: Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections.
Am J Pathol. 190 (6): 1188-210. -
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366. -
Matsuyama, S. et al. (2021) Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin.
J Vet Med Sci. 83 (9): 1435-42. -
Carroll, C.S.E. et al. (2021) Simple and effective bacterial-based intratumoral cancer immunotherapy.
J Immunother Cancer.9(9):e002688. -
Maciag, S.S. et al. (2022) The influence of source of porcine colostrum in development of early immune ontogeny in the piglet
Res Sq. Mar 24 [Epub ahead of print]. -
do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
Res Vet Sci. 145: 193-204. -
Korbonits, L. et al. (2022) Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome.
Metabolites. 12 (10): 924.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- MB-1
- RRID
- AB_905978
- UniProt
- P11912
- Entrez Gene
- CD79A
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0005771 multivesicular body
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0030183 B cell differentiation
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050853 B cell receptor signaling pathway
- View More GO Terms
View more products with CD79 specificity
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