CD79a antibody | HM57

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Mouse anti Human CD79a:Alexa Fluor® 488

Mouse anti Human CD79a:Alexa Fluor® 647

Mouse anti Human CD79a:FITC

Mouse anti Human CD79a

Mouse anti Human CD79a:RPE

Mouse anti Human CD79a:Alexa Fluor® 700

Product Type
Monoclonal Antibody
Clone
HM57
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2538A488 F* 100 Tests/1ml
MCA2538A647T F* 25 Tests/0.25ml
MCA2538A647 F* 100 Tests/1ml
MCA2538F F* 0.1 mg
MCA2538T C F* P* WB 25 µg
MCA2538GA C F* P* 0.1 mg
MCA2538 C F* P* WB 0.2 mg
MCA2538H C F* P* WB 1 ml
MCA2538PET F* 25 Tests
MCA2538PE F* 100 Tests
MCA2538A700 F* 100 Tests/1ml
Mouse anti Human CD79a antibody, clone HM57 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45 kDa protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells.

The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signaling following antigen-binding to surface immunoglobulin.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Dog
Rabbit
Horse
Pig
Monkey
Rat
Bovine
Guinea Pig
Fallow deer
American Bison
Red deer
Ferret
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to Alexa Fluor 700 - liquid
Reconstitution
Pack Size: 25 Tests
Reconstitute with 0.25ml distilled water
Pack Size: 100 Tests
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
Pack Size: 25 µg, 0.1 mg, 0.2 mg
0.09%Sodium Azide (NaN3)
Pack Size: 1 ml
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Carrier Free
Yes
Immunogen
Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
Pack Size: 25 µg, 0.1 mg, 0.2 mg
IgG concentration 1.0mg/ml
Pack Size: 1 ml
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 Tests
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Pack Size: 100 Tests
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of reconstitution.
Shelf Life
18 months from date of despatch

More Information

UniProt
P11912 Related reagents
Entrez Gene
CD79A Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0005771 multivesicular body
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0030183 B cell differentiation
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050853 B cell receptor signaling pathway
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD79a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
Flow Cytometry 1 1/5 1/10
Flow Cytometry 1 Neat
Flow Cytometry 1 1/10 Pack Size: 25 µg, 0.2 mg, 1 ml
1/50 Pack Size: 0.1 mg
1/50 Pack Size: 25 µg, 0.2 mg
1/100 Pack Size: 0.1 mg
1/25 Pack Size: 1 ml
Immunohistology - Frozen
Immunohistology - Paraffin 2 1/100 1/500
Western Blotting
Flow Cytometry 1 Neat
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 100 Tests/1ml F
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F
Mouse IgG1 Negative Control:Alexa Fluor® 700 MCA928A700 100 Tests/1ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD79a antibody

  1. Mason, D.Y. et al. (1991) The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
    J Immunol. 147 (11): 2474-82.
  2. Jones, M. et al. (1993) Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
    J Immunol. 150 (12): 5429-35.
  3. Christgau, M. et al. (1998) Characterization of immunocompetent cells in the diseased canine periodontium.
    J Histochem Cytochem. 46: 1443-54.
  4. Spaas, J.H. et al. (2013) Culture and characterisation of equine peripheral blood mesenchymal stromal cells.
    Vet J. 195 (1): 107-13.
  5. Nelson, D.D. et al. (2010) CD8(+)/perforin(+)/WC1(-) gammadelta T cells, not CD8(+) alphabeta T cells, infiltrate vasculitis lesions of American bison (Bison bison) with experimental sheep-associated malignant catarrhal fever.
    Vet Immunol Immunopathol. 136: 284-91.
  6. De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
    Cytometry A. 81 (4): 312-23.
  7. Long, H. et al. (2016) Polyostotic Lymphoma in a Ferret (Mustela putorius furo).
    J Comp Pathol. 154 (4): 341-4.
  8. Schinköthe J >et al. (2016) Characterization of tuberculous granulomas in different stages of progression and associated tertiary lymphoid tissue in goats experimentally infected with Mycobacterium avium subsp. hominissuis.
    Comp Immunol Microbiol Infect Dis. 47: 41-51.
  9. Bozkurt, Y.A., et al. (2014) Histological and immunohistological studies of the structure of lymph nodes in Kilis goats.
    Biotech Histochem. 89(6):440-5.
  10. Froment R & Bédard C (2016) Marked hyperphosphatasemia associated with an acute leukemia in a Great Dane.
    Vet Clin Pathol. Aug 18. [Epub ahead of print]
  11. Aresu, L. et al. (2015) Canine indolent and aggressive lymphoma: clinical spectrum with histologic correlation.
    Vet Comp Oncol. 13 (4): 348-62.
  12. Poggi, A. et al. (2015) Flow cytometric evaluation of ki67 for the determination of malignancy grade in canine lymphoma.
    Vet Comp Oncol. 13 (4): 475-80.
  13. Gelain ME et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
    Vet Immunol Immunopathol. 159 (1-2): 91-6.
  14. Paebst, F. et al. (2014) Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: an approach toward a standardized definition.
    Cytometry A. 85 (8): 678-87.
  15. De Schauwer, C. et al. (2014) Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources.
    Stem Cell Res Ther. 5 (1): 6.
  16. Claessen, C. et al. (2015) Equid herpesvirus 1 (EHV1) infection of equine mesenchymal stem cells induces a pUL56-dependent downregulation of select cell surface markers.
    Vet Microbiol. 176 (1-2): 32-9.
  17. Novacco, M. et al. (2015) Prognostic factors in canine acute leukaemias: a retrospective study.
    Vet Comp Oncol. Jan 26. [Epub ahead of print]
  18. Hillmann, A. et al. (2016) Comparative Characterization of Human and Equine Mesenchymal Stromal Cells: A Basis for Translational Studies in the Equine Model.
    Cell Transplant. 25 (1): 109-24.
  19. Moore, P.F. et al. (2013) Canine inflamed nonepitheliotropic cutaneous T-cell lymphoma: a diagnostic conundrum.
    Vet Dermatol. 24 (1): 204-11.e44-5.
  20. Nagata, K. et al. (2017) Epstein-Barr Virus Lytic Reactivation Activates B Cells Polyclonally and Induces Activation-Induced Cytidine Deaminase Expression: A Mechanism Underlying Autoimmunity and Its Contribution to Graves' Disease.
    Viral Immunol. Mar 23. [Epub ahead of print]
  21. Wessels, M. et al. (2017) Systemic necrotizing polyarteritis in three weaned lambs from one flock.
    J Vet Diagn Invest. May 1:1040638717709856. [Epub ahead of print]
  22. Uitterdijk, A. et al. (2017) Time course of VCAM-1 expression in reperfused myocardial infarction in swine and its relation to retention of intracoronary administered bone marrow-derived mononuclear cells.
    PLoS One. 12 (6): e0178779.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.