Mouse anti Human CD68 antibody, clone 514H12 recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.
- Target Species
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
Pack Size: 1 ml
Pack Size: 0.1 ml
<0.1% Sodium Azide (NaN3)
- Fusion protein corresponding to external domain of human CD68.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- GO Terms
integral to membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD68 antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose. N.B. The epitope recognised by clone 514H12 is sensitive to peroxide. It is therefore recommended that any peroxidase-blocking steps be performed after incubation with this antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human tonsil
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Immunohistology - Paraffin
Product Specific References
References for CD68 antibody
Hameed, A. et al. (1994) Immunohistochemical expression of CD68 antigen in human peripheral blood T cells.
Hum Pathol. 25 (9): 872-6.
Madden, L.R. et al. (2010) Proangiogenic scaffolds as functional templates for cardiac tissue engineering.
Proc Natl Acad Sci U S A. 107 (34): 15211-6.
Muthana, M. et al. (2011) Use of macrophages to target therapeutic adenovirus to human prostate tumors.
Cancer Res. 71 (5): 1805-15.
da Costa, C.E. et al. (2005) Presence of osteoclast-like multinucleated giant cells in the bone and nonostotic lesions of Langerhans cell histiocytosis.
J Exp Med. 201 (5): 687-93.
Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
J Transl Med. 4: 30.
Coury, F. et al. (2008) Langerhans cell histiocytosis reveals a new IL-17A-dependent pathway of dendritic cell fusion.
Nat Med. 14: 81-7.
Rodriguez-Agudo, D. et al. (2006) Localization of StarD5 cholesterol binding protein.
J Lipid Res. 47: 1168-75.
Achard, V. et al. (2007) Renin receptor expression in human adipose tissue.
Am J Physiol Regul Integr Comp Physiol. 292: R274-82.
Paulmyer-Lacroix, O. et al. (2006) Expression of adrenomedullin in adipose tissue of lean and obese women.
Eur J Endocrinol. 155: 177-85.
Hever, A. et al. (2007) Human endometriosis is associated with plasma cells and overexpression of B lymphocyte stimulator.
Proc Natl Acad Sci U S A. 104: 12451-6.
Silaghi, A. et al. (2007) Expression of adrenomedullin in human epicardial adipose tissue: role of coronary status.
Am J Physiol Endocrinol Metab. 293: E1443-50.
Kaibara, N. et al. (2008) Comparative histopathological analysis between tenosynovitis and joint synovitis in rheumatoid arthritis.
Histopathology. 52: 856-64.
Moskovszky, L. et al. (2009) Genomic instability in giant cell tumor of bone. A study of 52 cases using DNA ploidy, relocalization FISH, and array-CGH analysis.
Genes Chromosomes Cancer. 48: 468-79.
Angel, C.E. et al. (2007) CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
Int Immunol. 19: 1271-9.
de Vos van Steenwijk PJ et al. (2013) Tumor-infiltrating CD14-positive myeloid cells and CD8-positive T-cells prolong survival in patients with cervical carcinoma.
Int J Cancer. 133 (12): 2884-94.
Quispel, W.T. et al. (2016) Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis.
Oncoimmunology. 5 (8): e1164364.
Toh, M.L. et al. (2014) Bone- and cartilage-protective effects of a monoclonal antibody against colony-stimulating factor 1 receptor in experimental arthritis.
Arthritis Rheumatol. 66 (11): 2989-3000.
Schirmer, L. et al. (2014) Differential loss of KIR4.1 immunoreactivity in multiple sclerosis lesions.
Ann Neurol. 75 (6): 810-28.
Schrevel, M. et al. (2017) Autocrine expression of the epidermal growth factor receptor ligand heparin-binding EGF-like growth factor in cervical cancer.
Int J Oncol. 50 (6): 1947-54.
Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
PLoS One. 8 (5): e65121.
Bobosha, K. et al. (2014) T-cell regulation in lepromatous leprosy.
PLoS Negl Trop Dis. 8 (4): e2773.
van Poppel, P.C.M. et al. (2018) Inflammation in the subcutaneous adipose tissue does not attenuate endothelial function in subjects with diabetes mellitus and subjects with dyslipidaemia and hypertension: A cross-sectional study.
Endocrinol Diabetes Metab. 1 (3): e00020.
Yuan, A. et al. (2020) Histologic analysis of medication-related osteonecrosis of the jaw compared with antiresorptive-exposed bone and other infectious, inflammatory, and necrotic jaw diseases.
Oral Surg Oral Med Oral Pathol Oral Radiol. 129 (2): 133-40.
Durán, D. et al. (2020) Histological and Immunohistochemical Study of Wounds in Sheep Skin in Maggot Therapy by Using Protophormia terraenovae. (Diptera: Calliphoridae.) Larvae.
J Med Entomol. 57 (2): 369-76.
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