CD68 antibody | 514H12

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Mouse anti Human CD68

Product Type
Monoclonal Antibody
Clone
514H12
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1815
Datasheet
C IF P*
SDS
1 ml loader

MCA1815T
Datasheet
C IF P*
SDS
0.1 ml loader

Mouse anti Human CD68 antibody, clone 514H12 recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes.

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
Pack Size: 1 ml
0.09%Sodium Azide
Pack Size: 0.1 ml
<0.1% Sodium Azide (NaN3)
Immunogen
Fusion protein corresponding to external domain of human CD68.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P34810
Entrez Gene
CD68
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0005765 lysosomal membrane
GO:0010008 endosome membrane
Regulatory
For research purposes only

Applications of CD68 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/40 1/80
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose. N.B. The epitope recognised by clone 514H12 is sensitive to peroxide. It is therefore recommended that any peroxidase-blocking steps be performed after incubation with this antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human tonsil

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for CD68 antibody

  1. Hameed, A. et al. (1994) Immunohistochemical expression of CD68 antigen in human peripheral blood T cells.
    Hum Pathol. 25 (9): 872-6.
  2. Madden, L.R. et al. (2010) Proangiogenic scaffolds as functional templates for cardiac tissue engineering.
    Proc Natl Acad Sci U S A. 107 (34): 15211-6.
  3. Muthana, M. et al. (2011) Use of macrophages to target therapeutic adenovirus to human prostate tumors.
    Cancer Res. 71 (5): 1805-15.
  4. da Costa, C.E. et al. (2005) Presence of osteoclast-like multinucleated giant cells in the bone and nonostotic lesions of Langerhans cell histiocytosis.
    J Exp Med. 201 (5): 687-93.
  5. Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
    J Transl Med. 4: 30.
  6. Coury, F. et al. (2008) Langerhans cell histiocytosis reveals a new IL-17A-dependent pathway of dendritic cell fusion.
    Nat Med. 14: 81-7.
  7. Rodriguez-Agudo, D. et al. (2006) Localization of StarD5 cholesterol binding protein.
    J Lipid Res. 47: 1168-75.
  8. Achard, V. et al. (2007) Renin receptor expression in human adipose tissue.
    Am J Physiol Regul Integr Comp Physiol. 292: R274-82.
  9. Paulmyer-Lacroix, O. et al. (2006) Expression of adrenomedullin in adipose tissue of lean and obese women.
    Eur J Endocrinol. 155: 177-85.
  10. Hever, A. et al. (2007) Human endometriosis is associated with plasma cells and overexpression of B lymphocyte stimulator.
    Proc Natl Acad Sci U S A. 104: 12451-6.
  11. Silaghi, A. et al. (2007) Expression of adrenomedullin in human epicardial adipose tissue: role of coronary status.
    Am J Physiol Endocrinol Metab. 293: E1443-50.
  12. Kaibara, N. et al. (2008) Comparative histopathological analysis between tenosynovitis and joint synovitis in rheumatoid arthritis.
    Histopathology. 52: 856-64.
  13. Moskovszky, L. et al. (2009) Genomic instability in giant cell tumor of bone. A study of 52 cases using DNA ploidy, relocalization FISH, and array-CGH analysis.
    Genes Chromosomes Cancer. 48: 468-79.
  14. Angel, C.E. et al. (2007) CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
    Int Immunol. 19: 1271-9.
  15. de Vos van Steenwijk PJ et al. (2013) Tumor-infiltrating CD14-positive myeloid cells and CD8-positive T-cells prolong survival in patients with cervical carcinoma.
    Int J Cancer. 133 (12): 2884-94.
  16. Quispel, W.T. et al. (2016) Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis.
    Oncoimmunology. 5 (8): e1164364.
  17. Toh, M.L. et al. (2014) Bone- and cartilage-protective effects of a monoclonal antibody against colony-stimulating factor 1 receptor in experimental arthritis.
    Arthritis Rheumatol. 66 (11): 2989-3000.
  18. Schirmer, L. et al. (2014) Differential loss of KIR4.1 immunoreactivity in multiple sclerosis lesions.
    Ann Neurol. 75 (6): 810-28.
  19. Schrevel, M. et al. (2017) Autocrine expression of the epidermal growth factor receptor ligand heparin-binding EGF-like growth factor in cervical cancer.
    Int J Oncol. 50 (6): 1947-54.
  20. Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
    PLoS One. 8 (5): e65121.
  21. Bobosha, K. et al. (2014) T-cell regulation in lepromatous leprosy.
    PLoS Negl Trop Dis. 8 (4): e2773.
  22. van Poppel, P.C.M. et al. (2018) Inflammation in the subcutaneous adipose tissue does not attenuate endothelial function in subjects with diabetes mellitus and subjects with dyslipidaemia and hypertension: A cross-sectional study.
    Endocrinol Diabetes Metab. 1 (3): e00020.
  23. Yuan, A. et al. (2020) Histologic analysis of medication-related osteonecrosis of the jaw compared with antiresorptive-exposed bone and other infectious, inflammatory, and necrotic jaw diseases.
    Oral Surg Oral Med Oral Pathol Oral Radiol. 129 (2): 133-40.
  24. Durán, D. et al. (2020) Histological and Immunohistochemical Study of Wounds in Sheep Skin in Maggot Therapy by Using Protophormia terraenovae. (Diptera: Calliphoridae.) Larvae.
    J Med Entomol. 57 (2): 369-76.

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