CD68 antibody | 514H12
Mouse anti Human CD68 antibody, clone 514H12 (MCA1815) used to identify macrophages in human peritoneal tissue by immunofluorescence on cryostat sections.
Image caption:
Triple immunofluorescence costaining of frozen right peritoneal tissues were stained with CD68 (red), CD31 (blue), and keratin (CK) (green) antibodies (Rows 1 & 2) Row 1, peritoneal cells from a patient with EOC (ID 266 m) appear yellow from the colocalization of CD68 (red) and CK (green) on some surface mesothelial cells. CD31 staining (blue) indicates endothelial cells just under the mesothelium. Row 2, peritoneal cells from a patient with benign cystic teratoma of the ovary (ID 283b) show prominent staining for keratin in the single cell mesothelial layer but no staining for CD68 staining (red) and positive staining for endothelial cells (blue). Rows 3 and 4, peritoneal cells from patient ID#235 showed colocalization of CD68 (blue) and CD163 (green) appearing cyan color; CD68 (blue) and CXCL8 (red) costaining showed magenta effect and no color changed in CD163+ cells (green). Images were analyzed by confocal laser scanning microscopy (magnification 400×). H&E stained sections are shown for comparison.
From: Wang X, Deavers M, Patenia R, Bassett RL Jr, Mueller P, Ma Q, Wang E, Freedman RS.
Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
J Transl Med. 2006 Jul 6;4:30.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD68 antibody, clone 514H12 (MCA1815) used to identify macrophages in human peritoneal tissue by immunofluorescence on ascitic fluid cytospins.
Image caption:
Stained cytospin preps of mononuclear leukocytes isolated from ascitic fluid on F:H density cushion (patient ID 290; top row) or from frozen tissue (patient ID 235; bottom row) were fixed with 4% paraformaldehyde and double-stained with phospho-cPLA2 (Ser505) antibody (red) and CD68 (green) or CD163 (green). Indirect immunofluorescence showed pcPLA2 in both the nucleus and cytoplasm, and results were similar in the cells from ascites and those from tissue. Cells costained for CD68 and cPLA2 showed colocalization of both markers in the cytoplasm and in the nucleus (yellow). However, in CD163+ cells, cPLA2 was seen only in relation to the nucleus.
From: Wang X, Deavers M, Patenia R, Bassett RL Jr, Mueller P, Ma Q, Wang E, Freedman RS.
Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
J Transl Med. 2006 Jul 6;4:30.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD68 antibody, clone 514H12 (MCA1815) used to visualize macrophages in myeloid clusters by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
Prominent myeloid clusters associated with anthracosis and elevated STAT3 activity in NSCLC uninvolved LNs.
(A) HE staining of normal LNs from individuals without cancer (n = 4; left two panels) and uninvolved regional LNs from NSCLC patients (middle two panels) showing anthracotic carbon pigment (indicated with arrows; Scale bar, 20 μm) in patient LNs. Immunofluorescent staining for CD68 (right panel) demonstrating anthracotic pigment is within CD68+ cells (Note the black dots indicated with small arrows in CD68+ cells; Scale bar, 20 μm).
From: Zhang W, Pal SK, Liu X, Yang C, Allahabadi S, Bhanji S, et al. (2013)
Myeloid Clusters Are Associated with a Pro-Metastatic Environment and Poor Prognosis in Smoking-Related Early Stage Non-Small Cell Lung Cancer.
PLoS ONE 8(5): e65121.
doi: 10.1371/journal.pone.0065121
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD68 antibody, clone 514H12 (MCA1815) used to visualize macrophages in myeloid clusters by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
(B) IHC staining showing prominent CD68+ myeloid clusters associated with anthracosis and highly activated STAT3 in uninvolved NSCLC regional LNs (right panels) compared to normal LNs (n = 4; left panels) (Scale bar, 200 μm).
From: Zhang W, Pal SK, Liu X, Yang C, Allahabadi S, Bhanji S, et al. (2013)
Myeloid Clusters Are Associated with a Pro-Metastatic Environment and Poor Prognosis in Smoking-Related Early Stage Non-Small Cell Lung Cancer.
PLoS ONE 8(5): e65121.
doi: 10.1371/journal.pone.0065121
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD68 antibody, clone 514H12 (MCA1815) used to visualize macrophages in myeloid clusters by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
Myeloid clusters in uninvolved LNs colocalize with occult metastasis and cFn.
(B) Confocal images showing the colocalization of pan-CK+ NSCLC cells (arrows) and CD68+ myeloid clusters associated with anthracosis. (Scale bar, 50 μm) (C) Confocal images showing the colocalization of myeloid clusters associated with anthracosis (arrows) and cFn. (Scale bar, 50 μm).
From: Zhang W, Pal SK, Liu X, Yang C, Allahabadi S, Bhanji S, et al. (2013)
Myeloid Clusters Are Associated with a Pro-Metastatic Environment and Poor Prognosis in Smoking-Related Early Stage Non-Small Cell Lung Cancer.
PLoS ONE 8(5): e65121.
doi: 10.1371/journal.pone.0065121
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD68 antibody, clone 514H12 (MCA1815) used to visualize macrophages in myeloid clusters by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Scoring method.
H&E staining and IHC staining for CD68 showing representative images for the scoring of anthracosis intensity, anthracotic myeloid cluster infiltration (Scale bar, 200 μm).
From: Zhang W, Pal SK, Liu X, Yang C, Allahabadi S, Bhanji S, et al. (2013)
Myeloid Clusters Are Associated with a Pro-Metastatic Environment and Poor Prognosis in Smoking-Related Early Stage Non-Small Cell Lung Cancer.
PLoS ONE 8(5): e65121.
doi: 10.1371/journal.pone.0065121
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD68 antibody, clone 514H12 (MCA1815) used to illustrate macrophages in cervical tumor and stromal tissue sections by immunohistochemistry.
Image caption:
Immunohistochemical double staining of heparin-binding EGF-like growth factor (HB-EGF, DAB) and macrophages (CD68, PermaBlue), without nuclear counterstaining (A), and the simulated fluorescence composite image obtained through spectral imaging (B), showing not only macrophages (arrow), but also other cells in the stroma and tumor cells strongly expressing HB-EGF in cervical cancer.
From: Schrevel M, Osse EM, Prins FA, Trimbos JBMZ, Fleuren GJ, Gorter A, Jordanova ES.
Autocrine expression of the epidermal growth factor receptor ligand heparin-binding EGF-like growth factor in cervical cancer.
Int J Oncol. 2017 Jun;50(6):1947-54.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti human CD68 antibody, clone 514H12 (MCA1815) used to label macrophages in human renal cell carcinoma tumors by immunohistochemistry.
Image caption:
Enhanced infiltration of macrophages after VEGF-targeted therapy. a. Immunohistochemical detection of the total number of macrophages (CD68+) and the number of M2-like CD163+ macrophages in primary RCC tumor sections from patients treated with bevacizumab (n = 33) or sunitinib (n = 35) prior to cytoreductive surgery, as well as from untreated control tissues (n = 53). Scale bar represents 50 μm. Scatter plot quantifications of relative numbers of infiltrated CD68+ macrophages (b) and the subpopulation of M2-like CD163+ macrophages (c). Horizontal bars indicate the mean values for each patient group. Significance is indicated by asterisks (**p < 0.01 and ***p < 0.001)
From: Nowak-Sliwinska P, van Beijnum JR, Griffioen CJ, Huinen ZR, Sopesens NG, Schulz R, Jenkins SV, Dings RPM, Groenendijk FH, Huijbers EJM, Thijssen VLJL, Jonasch E, Vyth-Dreese FA, Jordanova ES, Bex A, Bernards R, de Gruijl TD, Griffioen AW.
Proinflammatory activity of VEGF-targeted treatment through reversal of tumor endothelial cell anergy.
Angiogenesis. 2022 Dec 2 [Epub ahead of print].
doi: 10.1007/s10456-022-09863-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IF P C ResetMouse anti Human CD68
- Product Type
- Monoclonal Antibody
- Clone
- 514H12
- Isotype
- IgG2a
- Specificity
- CD68
Antibody Quality and Performance Guarantee
| Mouse anti Human CD68 antibody, clone 514H12 recognizes the human CD68 cell surface antigen, a ~110 kDa glycoprotein primarily expressed by macrophages and monocytes. |
- Target Species
- Human
- Product Form
- Tissue culture supernatant - liquid
- Preservative Stabilisers
- Pack Size: 1 ml0.09% sodium azide (NaN3)Pack Size: 0.1 ml<0.1% sodium azide (NaN3)
- Immunogen
- Fusion protein corresponding to external domain of human CD68.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 1 | |
1/40 | 1/80 |
| Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose. N.B. The epitope recognised by clone 514H12 is sensitive to peroxide. It is therefore recommended that any peroxidase-blocking steps be performed after incubation with this antibody.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human tonsil
References for CD68 antibody
-
da Costa, C.E. et al. (2005) Presence of osteoclast-like multinucleated giant cells in the bone and nonostotic lesions of Langerhans cell histiocytosis.
J Exp Med. 201 (5): 687-93. -
Rodriguez-Agudo, D. et al. (2006) Localization of StarD5 cholesterol binding protein.
J Lipid Res. 47: 1168-75. -
Paulmyer-Lacroix, O. et al. (2006) Expression of adrenomedullin in adipose tissue of lean and obese women.
Eur J Endocrinol. 155: 177-85. -
Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
J Transl Med. 4: 30. -
Angel, C.E. et al. (2007) CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts.
Int Immunol. 19: 1271-9. -
Achard, V. et al. (2007) Renin receptor expression in human adipose tissue.
Am J Physiol Regul Integr Comp Physiol. 292: R274-82. -
Hever, A. et al. (2007) Human endometriosis is associated with plasma cells and overexpression of B lymphocyte stimulator.
Proc Natl Acad Sci U S A. 104: 12451-6. -
Silaghi, A. et al. (2007) Expression of adrenomedullin in human epicardial adipose tissue: role of coronary status.
Am J Physiol Endocrinol Metab. 293: E1443-50.
View The Latest Product References
-
Kaibara, N. et al. (2008) Comparative histopathological analysis between tenosynovitis and joint synovitis in rheumatoid arthritis.
Histopathology. 52: 856-64. -
Moskovszky, L. et al. (2009) Genomic instability in giant cell tumor of bone. A study of 52 cases using DNA ploidy, relocalization FISH, and array-CGH analysis.
Genes Chromosomes Cancer. 48: 468-79. -
Muthana, M. et al. (2011) Use of macrophages to target therapeutic adenovirus to human prostate tumors.
Cancer Res. 71 (5): 1805-15. -
de Vos van Steenwijk PJ et al. (2013) Tumor-infiltrating CD14-positive myeloid cells and CD8-positive T-cells prolong survival in patients with cervical carcinoma.
Int J Cancer. 133 (12): 2884-94. -
Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
PLoS One. 8 (5): e65121. -
Toh, M.L. et al. (2014) Bone- and cartilage-protective effects of a monoclonal antibody against colony-stimulating factor 1 receptor in experimental arthritis.
Arthritis Rheumatol. 66 (11): 2989-3000. -
Schirmer, L. et al. (2014) Differential loss of KIR4.1 immunoreactivity in multiple sclerosis lesions.
Ann Neurol. 75 (6): 810-28. -
Bobosha, K. et al. (2014) T-cell regulation in lepromatous leprosy.
PLoS Negl Trop Dis. 8 (4): e2773. -
Quispel, W.T. et al. (2016) Tertiary lymphoid structures are confined to patients presenting with unifocal Langerhans Cell Histiocytosis.
Oncoimmunology. 5 (8): e1164364. -
Schrevel, M. et al. (2017) Autocrine expression of the epidermal growth factor receptor ligand heparin-binding EGF-like growth factor in cervical cancer.
Int J Oncol. 50 (6): 1947-54. -
van Poppel, P.C.M. et al. (2018) Inflammation in the subcutaneous adipose tissue does not attenuate endothelial function in subjects with diabetes mellitus and subjects with dyslipidaemia and hypertension: A cross-sectional study.
Endocrinol Diabetes Metab. 1 (3): e00020. -
Yuan, A. et al. (2020) Histologic analysis of medication-related osteonecrosis of the jaw compared with antiresorptive-exposed bone and other infectious, inflammatory, and necrotic jaw diseases.
Oral Surg Oral Med Oral Pathol Oral Radiol. 129 (2): 133-40. -
Durán, D. et al. (2020) Histological and Immunohistochemical Study of Wounds in Sheep Skin in Maggot Therapy by Using Protophormia terraenovae. (Diptera: Calliphoridae.) Larvae.
J Med Entomol. 57 (2): 369-76. -
Nowak-Sliwinska, P. et al. (2022) Proinflammatory activity of VEGF-targeted treatment through reversal of tumor endothelial cell anergy.
Angiogenesis. Dec 02 [Epub ahead of print]. -
Cossins, B.C. et al. (2023) Sex-specific association between adipose tissue inflammation and vascular and metabolic complications of obesity.
J Clin Endocrinol Metab. dgad193.
- Synonyms
- Macrosialin
- RRID
- AB_322866
- UniProt
- P34810
- Entrez Gene
- CD68
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- GO:0005765 lysosomal membrane
- GO:0010008 endosome membrane
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