CD47 antibody | BRIC126
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Mouse anti human CD47 antibody, clone BRIC126 recognises the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52 kDa molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Bovine Sheep Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human erythrocytes
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
More Information
- UniProt
- Q08722 Related reagents
- Entrez Gene
- CD47 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0007229 integrin-mediated signaling pathway
- GO:0008284 positive regulation of cell proliferation
- GO:0022409 positive regulation of cell-cell adhesion
- GO:0034329 cell junction assembly
- GO:0050870 positive regulation of T cell activation
- GO:0050900 leukocyte migration
- GO:0070053 thrombospondin receptor activity
- Regulatory
- For research purposes only
Applications of CD47 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | ||
| Functional Assays 1 | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting 2 |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Our Slide-A-Lyzer® Dialysis Cassette (EQU003) is recommended suitable for this purpose.
- 2BRIC126 recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction, see Shahein et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | 100 Tests | F |
Product Specific References
References for CD47 antibody
-
Avent, N. et al. (1988) Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific.
Biochem J. 251 (2): 499-505. -
Campbell, I.G. et al. (1992) An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains.
Cancer Res. 52 (19): 5416-20. -
Brown, E. et al. (1990) Integrin-associated protein: a 50-kD plasma membrane antigen physically and functionally associated with integrins.
J Cell Biol. 111 (6 Pt 1): 2785-94. -
Lindberg, F.P. et al. (1993) Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding.
J Cell Biol. 123 (2): 485-96. -
Ide, K. et al. (2007) Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages.
Proc Natl Acad Sci U S A. 104 (12): 5062-6. -
Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes
and modulate CD18 expression.
Vet Res. 39: 50. -
Chao, M.P. et al. (2011) Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemia.
Cancer Res. 71 (4): 1374-84. -
Shahein, Y.E. et al. (2002) Molecular cloning and functional characterization of the pig homologue of integrin-associated protein (IAP/CD47).
Immunology. 106: 564-76. -
Brooke, G. et al. (2004) Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.
J Immunol. 173: 2562-70. -
Wewer, C. et al. (2011) Transcellular migration of neutrophil granulocytes through the blood-cerebrospinal fluid barrier after infection with Streptococcus suis.
J Neuroinflammation. 8: 51. -
Ticchioni, M. et al. (2001) Integrin-associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow.
FASEB J. 15: 341-50. -
Chao, M.P. et al. (2010) Anti-CD47 antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma.
Cell. 142: 699-713. -
Siddhartha, J et al. (2016) Methods of manipulating phagocytosis mediated by CD47.
US patent app 15/054930
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
