CD47 antibody | BRIC126

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Mouse anti Human CD47

Product Type
Monoclonal Antibody
Clone
BRIC126
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA911T C F FN* IP WB* 25 µg
MCA911 C F FN* IP WB* 0.2 mg
Mouse anti human CD47 antibody, clone BRIC126 recognises the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52 kDa molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Bovine
Sheep
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human erythrocytes
Approx. Protein Concentrations
IgG concentration 1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q08722 Related reagents
Entrez Gene
CD47 Related reagents
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0007229 integrin-mediated signaling pathway
GO:0008284 positive regulation of cell proliferation
GO:0022409 positive regulation of cell-cell adhesion
GO:0034329 cell junction assembly
GO:0050870 positive regulation of T cell activation
GO:0050900 leukocyte migration
GO:0070053 thrombospondin receptor activity
Regulatory
For research purposes only

Applications of CD47 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Functional Assays 1
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 2
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Our Slide-A-Lyzer® Dialysis Cassette (EQU003) is recommended suitable for this purpose.
  2. 2BRIC126 recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction, see Shahein et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2b Negative Control MCA691 100 Tests F

Application Based External Images

Flow Cytometry

Functional Assays

Western Blotting

Product Specific References

References for CD47 antibody

  1. Avent, N. et al. (1988) Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific.
    Biochem J. 251 (2): 499-505.
  2. Campbell, I.G. et al. (1992) An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains.
    Cancer Res. 52 (19): 5416-20.
  3. Brown, E. et al. (1990) Integrin-associated protein: a 50-kD plasma membrane antigen physically and functionally associated with integrins.
    J Cell Biol. 111 (6 Pt 1): 2785-94.
  4. Lindberg, F.P. et al. (1993) Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding.
    J Cell Biol. 123 (2): 485-96.
  5. Ide, K. et al. (2007) Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages.
    Proc Natl Acad Sci U S A. 104 (12): 5062-6.
  6. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
    Vet Res. 39: 50.
  7. Chao, M.P. et al. (2011) Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemia.
    Cancer Res. 71 (4): 1374-84.
  8. Shahein, Y.E. et al. (2002) Molecular cloning and functional characterization of the pig homologue of integrin-associated protein (IAP/CD47).
    Immunology. 106: 564-76.
  9. Brooke, G. et al. (2004) Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.
    J Immunol. 173: 2562-70.
  10. Wewer, C. et al. (2011) Transcellular migration of neutrophil granulocytes through the blood-cerebrospinal fluid barrier after infection with Streptococcus suis.
    J Neuroinflammation. 8: 51.
  11. Ticchioni, M. et al. (2001) Integrin-associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow.
    FASEB J. 15: 341-50.
  12. Chao, M.P. et al. (2010) Anti-CD47 antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma.
    Cell. 142: 699-713.
  13. Siddhartha, J et al. (2016) Methods of manipulating phagocytosis mediated by CD47.
    US patent app 15/054930

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.