CD47 antibody | BRIC126
Mouse anti Human CD47 antibody, clone BRIC126 (MCA911) used to assess levels of CD47 expression in serum samples from pigs by western blotting.
Image caption:
(A) Western blot of CD47 from each group at different time hours
From: Wang Z, Guo H, Xu R, Zhao CN, Xia Y.
The effects of cell surface CD47 downregulation on ischaemia-reperfusion injury during pig liver transplantation.
Int J Exp Pathol. 2021 Jun;102(3):140-7.
doi: 10.1111/iep.12391.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti human CD47 antibody, clone BRIC126 (MCA911) used to label CD47 expressing cells in buman brain biopsies by immunofluorescence.
Image caption:
Gene and protein expression of CD200, CD47, CD200R and SIRPα in NAGM
(E) Immunofluorescent staining of CD200 and MBP shows that CD200 expression was abundant in NAGM and low in NAWM. Scale bar on the overview image is 1 cm and on the zoomed image it is 250 μm. (F) CD200 expression co-localized with MBP+ myelin as indicated with arrows; scale bar is set at 10 μm. (G) CD200 expression co-localized with NFH+ axons as indicated with arrows; scale bar is set at 5 μm. (H) CD47 expression was, like CD200, abundant in NAGM and lower in NAWM. Scale bar on the overview image is 1 cm and on the zoomed image is 250 μm. (I) CD47 strongly co-localized with some MBP+ myelin, as indicated with the arrows, and was sometimes localized adjacent to the myelin, on the outer layer, as indicated with the arrowhead. Scale bar is set at 10 μm. (J) Occasionally, CD47 co-localized with NFH+ axons, scale bar is set at 5 μm.
From: van den Bosch AMR, Wever D, Schonewille P, Schuller SL, Smolders J, Hamann J, Huitinga I.
Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 2024 Aug 7;6(4):fcae264.
doi: 10.1093/braincomms/fcae264.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti human CD47 antibody, clone BRIC126 (MCA911) used to label CD47 expressing cells in buman brain biopsies by immunohistochemistry on cryostat sections.
Image caption:
Protein expression of CD200, CD47, CD200R and SIRPα in NAGM
DAB stainings showed (M) mainly extracellular deposition and sporadic expression on neurons for CD200, (N) mainly extracellular deposition and sporadic expression on neurons for CD47, (O) sporadic positive staining of cells for CD200R, and (P) light positive neurons and some darker stained round cells for SIRPα. Scale bars are set at 20 μm.
From: van den Bosch AMR, Wever D, Schonewille P, Schuller SL, Smolders J, Hamann J, Huitinga I.
Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 2024 Aug 7;6(4):fcae264.
doi: 10.1093/braincomms/fcae264.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD47
- Product Type
- Monoclonal Antibody
- Clone
- BRIC126
- Isotype
- IgG2b
- Specificity
- CD47
Antibody Quality and Performance Guarantee
| Mouse anti human CD47 antibody, clone BRIC126 recognises the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52 kDa molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Bovine Sheep Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human erythrocytes
- Approx. Protein Concentrations
- MCA911T: IgG concentration 1.0 mg/ml
- MCA911: IgG concentration 1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | |
| Functional Assays 1 | |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
||
| Western Blotting 2 | |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2BRIC126 recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction, see Shahein et al. for details.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG2b Negative Control | ||||||
Source Reference
-
Avent, N. et al. (1988) Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific.
Biochem J. 251 (2): 499-505.
References for CD47 antibody
-
Ticchioni, M. et al. (2001) Integrin-associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow.
FASEB J. 15: 341-50. -
Shahein, Y.E. et al. (2002) Molecular cloning and functional characterization of the pig homologue of integrin-associated protein (IAP/CD47).
Immunology. 106: 564-76. -
Brooke, G. et al. (2004) Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.
J Immunol. 173: 2562-70. -
Ide, K. et al. (2007) Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages.
Proc Natl Acad Sci U S A. 104 (12): 5062-6. -
Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
Vet Res. 39: 50. -
Chao, M.P. et al. (2010) Anti-CD47 antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma.
Cell. 142: 699-713. -
Wewer, C. et al. (2011) Transcellular migration of neutrophil granulocytes through the blood-cerebrospinal fluid barrier after infection with Streptococcus suis.
J Neuroinflammation. 8: 51. -
Chao, M.P. et al. (2011) Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemia.
Cancer Res. 71 (4): 1374-84.
View The Latest Product References
-
Petrova, P.S. et al. (2017) TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding.
Clin Cancer Res. 23 (4): 1068-79. -
Noda, G.S. et al. (2020) Specificities and isotypes of erythrocytes autoantibodies in patients with warm autoimmune hemolytic anemia
Rev Cubana Hematol Inmunol Hemoter 36(4): e1283. -
Kosaka, A. et al. (2021) CD47 blockade enhances the efficacy of intratumoral STING-targeting therapy by activating phagocytes.
J Exp Med. 218 (11): e20200792. -
Wang, Z. et al. (2021) The effects of cell surface CD47 downregulation on ischaemia-reperfusion injury during pig liver transplantation.
Int J Exp Pathol. 102 (3): 140-147. -
van den Bosch, A.M.R. et al. (2024) Cortical CD200-CD200R and CD47-SIRPα expression is associated with multiple sclerosis pathology.
Brain Commun. 6 (4): fcae264.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- SIRP LIGAND
- RRID
- AB_321436
- UniProt
- Q08722
- Entrez Gene
- CD47
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0007229 integrin-mediated signaling pathway
- GO:0008284 positive regulation of cell proliferation
- GO:0022409 positive regulation of cell-cell adhesion
- GO:0034329 cell junction assembly
- GO:0050870 positive regulation of T cell activation
- View More GO Terms
- GO:0050900 leukocyte migration
- GO:0070053 thrombospondin receptor activity
View more products with CD47 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up