CD47 antibody | BRIC126

Mouse anti Human CD47

Product Type
Monoclonal Antibody
Clone
BRIC126
Isotype
IgG2b
Specificity
CD47

Product Code Applications Pack Size List Price Your Price Qty
MCA911
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F FN * IP WB * 0.2 mg loader
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loader
MCA911T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F FN * IP WB * 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti human CD47 antibody, clone BRIC126 recognises the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52 kDa molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog
Bovine
Sheep
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
TRIS buffered glycine
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human erythrocytes
Approx. Protein Concentrations
Pack Size: 0.2 mg
IgG concentration 1 mg/ml
Pack Size: 25 µg
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Functional Assays 1
Immunohistology - Frozen
Immunoprecipitation
Western Blotting 2
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
  2. 2 BRIC126 recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction, see Shahein et al. for details
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD47 antibody

  1. Avent, N. et al. (1988) Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific.
    Biochem J. 251 (2): 499-505.
  2. Ide, K. et al. (2007) Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages.
    Proc Natl Acad Sci U S A. 104 (12): 5062-6.
  3. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
    Vet Res. 39: 50.
  4. Chao, M.P. et al. (2011) Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemia.
    Cancer Res. 71 (4): 1374-84.
  5. Shahein, Y.E. et al. (2002) Molecular cloning and functional characterization of the pig homologue of integrin-associated protein (IAP/CD47).
    Immunology. 106: 564-76.
  6. Brooke, G. et al. (2004) Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.
    J Immunol. 173: 2562-70.
  7. Wewer, C. et al. (2011) Transcellular migration of neutrophil granulocytes through the blood-cerebrospinal fluid barrier after infection with Streptococcus suis.
    J Neuroinflammation. 8: 51.
  8. Ticchioni, M. et al. (2001) Integrin-associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow.
    FASEB J. 15: 341-50.
  9. View The Latest Product References
  10. Chao, M.P. et al. (2010) Anti-CD47 antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma.
    Cell. 142: 699-713.
  11. Siddhartha, J et al. (2016) Methods of manipulating phagocytosis mediated by CD47.
    US patent app 15/054930
  12. Petrova, P.S. et al. (2017) TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding.
    Clin Cancer Res. 23 (4): 1068-79.
  13. Noda, G.S. et al. (2020) Specificities and isotypes of erythrocytes autoantibodies in patients with warm autoimmune hemolytic anemia
    Rev Cubana Hematol Inmunol Hemoter 36(4): e1283.
  14. Kosaka, A. et al. (2021) CD47 blockade enhances the efficacy of intratumoral STING-targeting therapy by activating phagocytes.
    J Exp Med. 218 (11): e20200792.
  15. Wang, Z. et al. (2021) The effects of cell surface CD47 downregulation on ischaemia-reperfusion injury during pig liver transplantation.
    Int J Exp Pathol. 102 (3): 140-147.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Flow Cytometry

Functional Assays

Western Blotting

Synonyms
SIRP LIGAND
RRID
AB_321436
UniProt
Q08722
Entrez Gene
CD47
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0007229 integrin-mediated signaling pathway
GO:0008284 positive regulation of cell proliferation
GO:0022409 positive regulation of cell-cell adhesion
GO:0034329 cell junction assembly
GO:0050870 positive regulation of T cell activation
GO:0050900 leukocyte migration
GO:0070053 thrombospondin receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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