CD47 antibody | BRIC126
Mouse anti Human CD47 antibody, clone BRIC126 (MCA911) used to assess levels of CD47 expression in serum samples from pigs by western blotting.
Image caption:
(A) Western blot of CD47 from each group at different time hours
From: Wang Z, Guo H, Xu R, Zhao CN, Xia Y.
The effects of cell surface CD47 downregulation on ischaemia-reperfusion injury during pig liver transplantation.
Int J Exp Pathol. 2021 Jun;102(3):140-7.
doi: 10.1111/iep.12391.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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WB ResetMouse anti Human CD47
- Product Type
- Monoclonal Antibody
- Clone
- BRIC126
- Isotype
- IgG2b
- Specificity
- CD47
Antibody Quality and Performance Guarantee
| Mouse anti human CD47 antibody, clone BRIC126 recognises the human CD47 cell surface glycoprotein, a heavily N-glycosylated 47-52 kDa molecule. CD47 is expressed on all cells and tissues so far examined, although expression is reduced on erythrocytes of the rare Rh null phenotype. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Bovine Sheep Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- TRIS buffered glycine
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human erythrocytes
- Approx. Protein Concentrations
- Pack Size: 0.2 mgIgG concentration 1 mg/mlPack Size: 25 µgIgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | |
| Functional Assays 1 | |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
||
| Western Blotting 2 | |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2 BRIC126 recognizes CD47 under non-reducing conditions, the CD47 epitope recognized is lost on reduction, see Shahein et al. for details
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control | ||||||
Source Reference
-
Avent, N. et al. (1988) Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific.
Biochem J. 251 (2): 499-505.
References for CD47 antibody
-
Ticchioni, M. et al. (2001) Integrin-associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow.
FASEB J. 15: 341-50. -
Shahein, Y.E. et al. (2002) Molecular cloning and functional characterization of the pig homologue of integrin-associated protein (IAP/CD47).
Immunology. 106: 564-76. -
Brooke, G. et al. (2004) Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family.
J Immunol. 173: 2562-70. -
Ide, K. et al. (2007) Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages.
Proc Natl Acad Sci U S A. 104 (12): 5062-6. -
Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression.
Vet Res. 39: 50. -
Chao, M.P. et al. (2010) Anti-CD47 antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma.
Cell. 142: 699-713. -
Wewer, C. et al. (2011) Transcellular migration of neutrophil granulocytes through the blood-cerebrospinal fluid barrier after infection with Streptococcus suis.
J Neuroinflammation. 8: 51. -
Chao, M.P. et al. (2011) Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemia.
Cancer Res. 71 (4): 1374-84.
View The Latest Product References
-
Petrova, P.S. et al. (2017) TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding.
Clin Cancer Res. 23 (4): 1068-79. -
Noda, G.S. et al. (2020) Specificities and isotypes of erythrocytes autoantibodies in patients with warm autoimmune hemolytic anemia
Rev Cubana Hematol Inmunol Hemoter 36(4): e1283. -
Kosaka, A. et al. (2021) CD47 blockade enhances the efficacy of intratumoral STING-targeting therapy by activating phagocytes.
J Exp Med. 218 (11): e20200792. -
Wang, Z. et al. (2021) The effects of cell surface CD47 downregulation on ischaemia-reperfusion injury during pig liver transplantation.
Int J Exp Pathol. 102 (3): 140-147.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- SIRP LIGAND
- RRID
- AB_321436
- UniProt
- Q08722
- Entrez Gene
- CD47
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0007229 integrin-mediated signaling pathway
- GO:0008284 positive regulation of cell proliferation
- GO:0022409 positive regulation of cell-cell adhesion
- GO:0034329 cell junction assembly
- GO:0050870 positive regulation of T cell activation
- View More GO Terms
- GO:0050900 leukocyte migration
- GO:0070053 thrombospondin receptor activity
View more products with CD47 specificity
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