CD41 antibody | PM6/248

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Anti Human CD41 Antibody, clone PM6/248 gallery image 1 — Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD61 (MCA2588A647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD61 (MCA2588A647) and FITC conjugated Mouse anti Human CD41 (**MCA467F**). All experiments performed on human blood gated on live, single cell platelets in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer

Mouse anti Human CD41:Alexa Fluor® 647

Mouse anti Human CD41:Preservative Free

Mouse anti Human CD41:FITC

Mouse anti Human CD41

Mouse anti Human CD41:RPE

Product Type: Monoclonal Antibody
Clone: PM6/248
Isotype: IgG1

Product Code	Applications	Pack Size
MCA467A647T	F	25 Tests/0.25ml
MCA467A647	F	100 Tests/1ml
MCA467XZ	F	1 mg
MCA467F	F	0.1 mg
MCA467GT	F	25 µg
MCA467GA	F	0.1 mg
MCA467G	F	0.2 mg
MCA467PET	F	25 Tests
MCA467PE	F	100 Tests

Mouse anti Human CD41 antibody, clone PM6/248 recognizes the human CD41 cell surface antigen, a ~140 kDa glycoprotein expressed by platelets and megakaryocytes. CD41 is also known as platelet glycoprotein IIb, and functions as a receptor for fibrinogen, fibronectin and vWF.

It has not been established if clone PM6/248 recognizes free CD41 or CD41 only when complexed with CD61. However, antibody binding is reduced in the presence of EDTA suggesting that the epitope recognized is dependent upon an intact CD41/61 complex.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Baboon
Mink
Mustelid

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Pack Size: 25 Tests

Reconstitute in 0.25 ml disilled water

Pack Size: 100 Tests

Reconstitute with 1 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

None present

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%

Sodium Azide

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Carrier Free

Yes

Carrier Free

Yes

Immunogen

Human platelet plasma membranes.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: P08514 Related reagents
Entrez Gene: ITGA2B Related reagents
GO Terms: GO:0002576 platelet degranulation; GO:0004872 receptor activity; GO:0008305 integrin complex; GO:0007229 integrin-mediated signaling pathway; GO:0007411 axon guidance; GO:0042802 identical protein binding; GO:0030168 platelet activation; GO:0031092 platelet alpha granule membrane
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of CD41 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/5
Flow Cytometry	1/100	1/200
Western Blotting
Flow Cytometry	Neat	1/10
Flow Cytometry
Western Blotting
Flow Cytometry		Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 100ul human whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 100ul whole blood.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood
Flow Cytometry: Use 10ul of the suggested working dilution to label 100ul whole blood.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	100 Tests/1ml	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:FITC	MCA928F	100 Tests	F
Mouse IgG1 Negative Control	MCA928	100 Tests	F
Mouse IgG1 Negative Control:RPE	MCA928PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD41 antibody

Hornby, E.J. et al. (1991) Activation of human platelets by exposure to a monoclonal antibody, PM6/248, to glycoprotein IIb-IIIa.
Br J Haematol. 79 (2): 277-85.
Michelson, A.D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons.
Leucocyte Typing V. Oxford University Press p 1230-1231.
Maloney, S.F. ety al. (2010) P2Y12 or P2Y1 inhibitors reduce platelet deposition in a microfluidic model of thrombosis while apyrase lacks efficacy under flow conditions.
Integr Biol (Camb). 2: 183-92.
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37.
Massoudy, P. et al. (2001) Evidence for inflammatory responses of the lungs during coronary artery bypass grafting with cardiopulmonary bypass.
Chest. 119: 31-6.
Kahng, J. et al. (2008) Quantitative comparisons of antibody-binding sites of platelet glycoprotein IIb/IIIa in aplastic anemia and idiopathic thrombocytopenic purpura.
Ann Clin Lab Sci. 38: 6-11.
Chae, H. et al. (2009) EDTA Inhibits the Binding of Clone 96.2C1, an Anti-CD41a Monoclonal Antibody, to the Platelets and Addition of Heparin and CaCl2 to the Antibody Neutralizes the EDTA-induced Inhibitory Effect
Korean J Hematol 44: 42 - 6.
Zahler, S. et al. (1999) Acute cardiac inflammatory responses to postischemic reperfusion during cardiopulmonary bypass.
Cardiovasc Res. 41: 722-30.
Wang, J.S. et al. (2005) Effects of exercise training and deconditioning on platelet aggregation induced by alternating shear stress in men.
Arterioscler Thromb Vasc Biol. 25: 454-60.
Welsh, J.D. et al. (2012) Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse.
J Thromb Haemost. 10 (11): 2344-53.
Kamat, V. et al. (2015) Microfluidic assessment of functional culture-derived platelets in human thrombi under flow.
Exp Hematol. 43 (10): 891-900.e4.
Vučetić, D. et al. (2018) Flow cytometry analysis of platelet populations: usefulness for monitoringthe storage lesion in pooled buffy-coat platelet concentrates.
Blood Transfus. 16 (1): 83-92.
Foruzanmehr, M. et al. (2014) Nano-structure TiO₂ film coating on 316L stainless steel via sol-gel technique for blood compatibility improvement
Nanomedicine J. 1 (3): 128-36.

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