CD41 antibody | PM6/248

Mouse anti Human CD41

Product Type
Monoclonal Antibody
Clone
PM6/248
Isotype
IgG1
Specificity
CD41

Product Code Applications Pack Size List Price Your Price Qty
MCA467G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.2 mg loader
List Price Your Price
loader
MCA467GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
MCA467GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD41 antibody, clone PM6/248 recognizes the human CD41 cell surface antigen, a ~140 kDa glycoprotein expressed by platelets and megakaryocytes. CD41 is also known as platelet glycoprotein IIb, and functions as a receptor for fibrinogen, fibronectin and vWF.

It has not been established if clone PM6/248 recognizes free CD41 or CD41 only when complexed with CD61. However, antibody binding is reduced in the presence of EDTA suggesting that the epitope recognized is dependent upon an intact CD41/61 complex.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Baboon
Mink
Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA467G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA467GA, MCA467GT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human platelet plasma membranes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

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Description Mouse IgG1 Negative Control

References for CD41 antibody

  1. Hornby, E.J. et al. (1991) Activation of human platelets by exposure to a monoclonal antibody, PM6/248, to glycoprotein IIb-IIIa.
    Br J Haematol. 79 (2): 277-85.
  2. Michelson, A.D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons.
    Leucocyte Typing V. Oxford University Press p 1230-1231.
  3. Maloney, S.F. ety al. (2010) P2Y12 or P2Y1 inhibitors reduce platelet deposition in a microfluidic model of thrombosis while apyrase lacks efficacy under flow conditions.
    Integr Biol (Camb). 2: 183-92.
  4. Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
    Vet Immunol Immunopathol. 119: 27-37.
  5. Massoudy, P. et al. (2001) Evidence for inflammatory responses of the lungs during coronary artery bypass grafting with cardiopulmonary bypass.
    Chest. 119: 31-6.
  6. Kahng, J. et al. (2008) Quantitative comparisons of antibody-binding sites of platelet glycoprotein IIb/IIIa in aplastic anemia and idiopathic thrombocytopenic purpura.
    Ann Clin Lab Sci. 38: 6-11.
  7. Chae, H. et al. (2009) EDTA Inhibits the Binding of Clone 96.2C1, an Anti-CD41a Monoclonal Antibody, to the Platelets and Addition of Heparin and CaCl2 to the Antibody Neutralizes the EDTA-induced Inhibitory Effect
    Korean J Hematol 44: 42 - 6.
  8. Zahler, S. et al. (1999) Acute cardiac inflammatory responses to postischemic reperfusion during cardiopulmonary bypass.
    Cardiovasc Res. 41: 722-30.
  9. View The Latest Product References
  10. Wang, J.S. et al. (2005) Effects of exercise training and deconditioning on platelet aggregation induced by alternating shear stress in men.
    Arterioscler Thromb Vasc Biol. 25: 454-60.
  11. Welsh, J.D. et al. (2012) Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse.
    J Thromb Haemost. 10 (11): 2344-53.
  12. Kamat, V. et al. (2015) Microfluidic assessment of functional culture-derived platelets in human thrombi under flow.
    Exp Hematol. 43 (10): 891-900.e4.
  13. Vučetić, D. et al. (2018) Flow cytometry analysis of platelet populations: usefulness for monitoringthe storage lesion in pooled buffy-coat platelet concentrates.
    Blood Transfus. 16 (1): 83-92.
  14. Foruzanmehr, M. et al. (2014) Nano-structure TiO2 film coating on 316L stainless steel via sol-gel technique for blood compatibility improvement
    Nanomedicine J. 1 (3): 128-36.
  15. Miyazaki, K. et al. (2015) Immature platelet fraction measurement is influenced by platelet size and is a useful parameter for discrimination of macrothrombocytopenia.
    Hematology. 20 (10): 587-92.
  16. Burdorf, L. et al. (2019) Thromboxane and histamine mediate PVR elevation during xenogeneic pig lung perfusion with human blood.
    Xenotransplantation. 26 (2): e12458.

Flow Cytometry

Synonyms
INTEGRIN ALPHA IIB
RRID
AB_324000
UniProt
P08514
Entrez Gene
ITGA2B
GO Terms
GO:0002576 platelet degranulation
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0030168 platelet activation
GO:0031092 platelet alpha granule membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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