CD4 antibody | RPA-T4
![Anti Human CD4 Antibody, clone RPA-T4 thumbnail image 1](https://images.bio-rad-antibodies.com/monoclonal/human-cd4-antibody-rpa-t4-mca1267-image1-52w.jpg)
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Mouse anti Human CD4
- Product Type
- Monoclonal Antibody
- Clone
- RPA-T4
- Isotype
- IgG1
- Specificity
- CD4
Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a ~55 kDa cell surface glycoprotein, primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping shows that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule. Mouse anti human CD4 antibody, clone RPA-T4 blocks gp120-CD4 interaction and inhibits syncytium formation (Piatier-Tonneau et al, 1997). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- MCA1267T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA1267, MCA1267GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human PHA blasts
- Approx. Protein Concentrations
- MCA1267T: IgG concentration 1mg/ml
- MCA1267, MCA1267GA: IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/50 | 1/100 |
Immunohistology - Frozen 1 | ![]() |
1/10 | 1/100 |
Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
- Histology Positive Control Tissue
- Human lymphatic tissue
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
List Price | Your Price | ||||||
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Description | Mouse IgG1 Negative Control |
References for CD4 antibody
-
Piatier-Tonneau, D. (1997) CD4 workshop panel report.
In: Leucocyte Typing VI: White Cell Differentiation Antigens: Proceedings of the Sixth International Workshop and Conference Held in Kobe, Japan, 10-14 November 1996. Garland Pub., 1998. -
Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes.
J Immunol. 167 (8): 4593-9. -
Wright, G.J. et al. (2001) The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans.
Immunology. 102 (2): 173-9. -
Pentón-Rol, G. et al. (2011) C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells.
Int Immunopharmacol. 11 (1): 29-38. -
Zhang, Y. et al. (2013) Accelerated in vivo. proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.
PLoS Pathog. 9 (4): e1003310. -
Bughani, U. et al. (2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.
PLoS One. 12 (7): e0180088. -
Agrawal, S.M. et al. (2013) Extracellular matrix metalloproteinase inducer shows active perivascular cuffs in multiple sclerosis.
Brain. 136 (Pt 6): 1760-77. -
Malmassari, S.L. et al. (2007) Impact of hepatitis B virus basic core promoter mutations on T cell response to an immunodominant HBx-derived epitope.
Hepatology. 45 (5): 1199-209.
View The Latest Product References
-
Wooldridge, L. et al. (2006) Anti-coreceptor antibodies profoundly affect staining with peptide-MHC class I and class II tetramers.
Eur J Immunol. 36 (7): 1847-55. -
Wildum, S. et al. (2006) Contribution of Vpu, Env, and Nef to CD4 down-modulation and resistance of human immunodeficiency virus type 1-infected T cells to superinfection.
J Virol. 80 (16): 8047-59. -
Kirchhof, J. et al. (2018) Learned immunosuppressive placebo responses in renal transplant patients.
Proc Natl Acad Sci U S A. 115 (16): 4223-7. -
Kelleher, M. et al. (2011) Comparative Kinetics of Immune Responses and Changes in Cellular Sub-Sets Detected in Colorectal Cancer Patients Vaccinated with MVA-5T4 (TroVax) Administered Alongside Two Different Chemotherapy Regimens
J Cancer Therapy. 02 (01): 54-64.
- RRID
- AB_321275
- UniProt
- P01730
- Entrez Gene
- CD4
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005201 extracellular matrix structural constituent
- GO:0005769 early endosome
- GO:0005789 endoplasmic reticulum membrane
- GO:0006948 induction by virus of host cell-cell fusion
- View More GO Terms
- GO:0006955 immune response
- GO:0008270 zinc ion binding
- GO:0015026 coreceptor activity
- GO:0019059 initiation of viral infection
- GO:0019901 protein kinase binding
- GO:0030260 entry into host cell
- GO:0031295 T cell costimulation
- GO:0032507 maintenance of protein location in cell
- GO:0042101 T cell receptor complex
- GO:0042289 MHC class II protein binding
- GO:0042803 protein homodimerization activity
- GO:0045058 T cell selection
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045234 protein palmitoleylation
- GO:0045860 positive regulation of protein kinase activity
- GO:0050690 regulation of defense response to virus by virus
- GO:0050852 T cell receptor signaling pathway
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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