CD31 antibody | WM59
Mouse anti Human CD31 antibody, clone WM59 (MCA1738) used for the detection of CD31 positive cells by flow cytometry.
Image caption:
Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors. (A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-β, RGS5, CD146, Nestin, α-SMA and NG2). They do not express hematopoietic (CD45), endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers (CD56), (green = isotype, red = respective marker).
From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012)
The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.
PLoS ONE 7(4): e35577.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD31 antibody, clone WM59 (MCA1738) used for the detection of CD31 positive cells by flow cytometry.
Image caption:
Characterisation of clonal perivascular MSC progenitor lines. (A) Single cell in vitro a few hours after plating, and culture at 7 and 21 days, respectively. Scale bar 50 μm. (B) Clonally derived MSC show the same antigen profile as detected for polyclonal lines with pericyte and MSC markers but neither neuronal nor glial precursor cell markers (O4, GFAP) (example here cortical clone; green = isotype, red = respective marker).(C) QPCR comparing clones derived from the ventricular zone (n = 2), and the cortex (n = 2) to a human neuronal progenitor cell line MesC2.10. Data show the relative higher expression of mRNA for RGS5, PDGFR-β and α-SMA in the MSC clones compared to the control. As expected, Nestin mRNA was present in perivascular MSC clones and neural progenitors at comparable amounts. NG2 mRNA was slightly higher expressed in the MSC clones compared to the control. (D) Importantly, neural progenitors markers such as CD133, SOX1, NGN2, PAX6 and Musashi were highly expressed in the positive control but not detectable in MSC clones.
From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012)
The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells.
PLoS ONE 7(4): e35577.
This image is from an open access article distributed under the terms of Creative Commons Attribution License.
Mouse anti Human CD31 monoclonal antibody, clone WM59 (MCA1738) used for the detection of CD31 expressing lung endothelium by immunofluorescence
Image caption:
Figure 7. Apigenin reduces LPS-induced apoptosis in the lungs. Lungs from mice treated with LPS, control, apigenin (Api) or apigenin 3 h prior to LPS (Api + LPS) were obtained at 24 h. (A) The number of apoptotic cells was determined by the TUNEL assay and expressed as the mean ± SEM of the numbers of stained cells per 1000× microscopic field (cpf) (N = 4 mice for each biological condition; * p < 0.01 vs. LPS), scale bars: 25 μm for all figures; (B,C) Caspase-3 activity was determined by the DEVD-AFC assay using lung lysates as described in materials and methods. Values represent the means ± SEM (N = 4 mice for each biological condition; * p < 0.05 vs. LPS); (D) Lung tissue immuno-histochemistry using antibodies specific for active caspase-3 in combination with anti-CD31, anti-cytokeratin or anti-7/4 antibodies specific for endothelia, epithelia and neutrophils, respectively. Scale bars, 100 μm.
From: Cardenas, H.; Arango, D.; Nicholas, C.; Duarte, S.; Nuovo, G.J.; He, W.; Voss, O.H.; Gonzalez-Mejia, M.E.; Guttridge, D.C.; Grotewold, E.; Doseff, A.I.
Dietary Apigenin Exerts Immune-Regulatory Activity in Vivo by Reducing NF-κB Activity, Halting Leukocyte Infiltration and Restoring Normal Metabolic Function.
Int. J. Mol. Sci. 2016, 17, 323.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD31 monoclonal antibody, clone WM59 (MCA17738) used for the detection of CD31 expressing cells in cardiovacular procegitor cell cultures by immunofluorescence.
Image caption:
In vitro differentiation of ISL1+ CVP clones into cardiomyocytes, as well as endothelial and smooth muscle cells. Undifferentiated ISL1+ CVP clones were maintained in medium supplemented with EDN1 and WNT3A. Differentiation of the CVPs into various cardiac lineages was achieved using protocols described under material and method. (a) Immunocytochemistry demonstrating the presence of endothelial cell (EC; CD31+), smooth muscle cell (SM; SMMHC+) and cardiomyocyte (CM; CTNT+) on differentiation with various differentiation media. EC was stained with mouse anti-CD31 primary antibody while SM and CM were stained with mouse anti-SMMHC and mouse anti-CTNT, respectively, at 1:100 dilutions. Cell nuclei (blue) were stained with 4,6-diamindino-2-phenylindole (DAPI). Scale bar, 100 μm. (b) Graphical representation illustrating differentiation efficiencies of ISL1+ CVP into various cardiac lineages. Flow cytometric analyses for EC (CD31+), SM (SMMHC+) and CM (CTNT+) differentiation were performed on two progenitor clones (clones 1 and 2). Bars, s.d. of n=3 experiments. *P<0.05 and **P<0.001, evaluated by Student's t-test. (c–e) Quantitative PCR confirming the expression of CD31 and VWF (EC markers); SM22 and SMMHC (SM markers); and NKX2.5, GATA4, MEF2C, TBX5, CX43 and TNNT2 (CM markers) when ISL1+ CVP clones were differentiated into the various cardiac lineages. Gene expressions were normalized to the respective undifferentiated ISL1+ CVP clones. n=3 experiments. *P<0.05 and **P<0.01, evaluated by Student's t-test.
From: Soh BS, Ng SY, Wu H, Buac K, Park JH, Lian X, Xu J, Foo KS, Felldin U, He X, Nichane M, Yang H, Bu L, Li RA, Lim B, Chien KR.
Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells.
Nat Commun. 2016 Mar 8;7:10774.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD31 monoclonal antibody, clone WM59 (MCA1738) used for the detection of CD31 expressing cells in cardiovacular procegitor cell cultures by immunofluorescence.
Image caption:
ISL1+ CVP differentiates and forms human–mouse chimeric circulatory system in vivo. (a) Xenografts stained positive for GFP present in kidney capsule and adult mouse heart after myocardial infarction (MI), demonstrating the engraftment of ISL1+ CVP-derived cells 3 weeks post transplantation. Cell nuclei (blue) were stained with 4,6-diamindino-2-phenylindole (DAPI). Scale bar, 100 μm. (b) Representative images of xenograft showing engraftment of ISL1+ CVP-derived differentiated cells in the kidney capsule and myocardium. Vasculature network could be seen from the surface of the xenograft (kidney capsule), with numerous blood vessels carrying red blood cells (RBCs) as shown in the haematoxylin and eosin (H&E) and Masson stains (indicated with black arrows). Site of MI is marked by red arrow (lower panel). Connective tissues revealed by Masson stain (stained blue) were observed throughout the xenograft. Enlarged images show functional blood vessels carrying blood cells. RV, right ventricle; LV, left ventricle. Scale bar, 100 μm. (c) Representative immunofluorescent image of muscularized blood vessels in xenografts (both kidney capsule and myocardium). Rings of endothelial cells were stained positive for CD31 (stained red). The arteries were surrounded by additional ring of smooth muscle cells that were stained positive for α-SMA. Cell nuclei (blue) were stained with DAPI. Scale bar, 50 μm. (d) Graphical representation of the numbers and sizes of blood vessels observed in the xenograft per field (transplantation into myocardium after MI). Results were tabulated from 10 random fields of H&E stains of the xenografts. Bars, s.d. of n=3 sections. D, diameter. *P<0.05 and **P<0.001, evaluated by Student's t-test. (e) Large coronary-like artery developed from the transplanted ISL1+ CVP in the kidney capsule. Immunofluorescent staining of the section revealed an inner layer of endothelial cells (stained positive for CD31), which is surrounded by a thick layer of smooth muscle cells that were stained positive for both SMMHC and α-SMA. Connective tissue surrounding the artery was further depicted by the presence of fibrillin-1 (FBN1) as well as Masson stain (blue). Cell nuclei (blue) were stained with DAPI. Scale bar, 100 μm. Scale bar in enlarged image, 50 &mum.
From: Soh BS, Ng SY, Wu H, Buac K, Park JH, Lian X, Xu J, Foo KS, Felldin U, He X, Nichane M, Yang H, Bu L, Li RA, Lim B, Chien KR.
Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells.
Nat Commun. 2016 Mar 8;7:10774.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD31:Alexa Fluor® 647
Mouse anti Human CD31:Biotin
Mouse anti Human CD31:FITC
Mouse anti Human CD31:Pacific Blue®
Mouse anti Human CD31
Mouse anti Human CD31:RPE
Mouse anti Human CD31:Alexa Fluor® 700
Mouse anti Human CD31:Starbright Blue 700
Mouse anti Human CD31:Starbright Violet 440
Mouse anti Human CD31:Starbright Violet 515
Mouse anti Human CD31:Starbright Violet 610
- Product Type
- Monoclonal Antibody
- Clone
- WM59
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1738A647T | F | 25 Tests/0.25ml |
|
|
| MCA1738A647 | F | 100 Tests/1ml |
|
|
| MCA1738B | F | 0.1 mg |
|
|
| MCA1738FT | F | 25 µg |
|
|
| MCA1738F | F | 0.1 mg |
|
|
| MCA1738PB | F | 100 Tests/1ml |
|
|
| MCA1738T | F IP | 25 µg |
|
|
| MCA1738 | F IF IP | 0.2 mg |
|
|
| MCA1738PE | F | 100 Tests |
|
|
| MCA1738PET | F | 25 Tests |
|
|
| MCA1738A700 | F | 100 Tests/1ml |
|
|
| MCA1738SBB700 | F | 100 Tests/0.5ml |
|
|
| MCA1738SBV440 | F | 100 Tests/0.5ml |
|
|
| MCA1738SBV515 | F | 100 Tests/0.5ml |
|
|
| MCA1738SBV610 | F | 100 Tests/0.5ml |
|
Mouse anti Human CD31 monoclonal antibody, clone WM59 recognizes the human CD31 antigen, a ~130 kDa single pass type I transmembrane glycoprotein bearing six C2 immunoglobulin domains. CD31 is expressed by all continuous endothelia including arteries, veins and non-sinusoidal capillaries, platelets,granulocytes and some lymphocytes. CD31 is not expressed by discontinuous endothelia such as hepatic sinusoids and splenic red pulp (Muller et al. 1989).CD31 is also known as PECAM-1.
The binding epitope for mouse anti human CD31, clone WM59 has been mapped to the Ig-like domain 2 (Fawcett et al. 1995).
The binding epitope for mouse anti human CD31, clone WM59 has been mapped to the Ig-like domain 2 (Fawcett et al. 1995).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cynomolgus monkey Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor®647- liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor 700 - liquid
- Product Form
- Purified IgG conjugated to StarBright Blue 700 - liquid
- Product Form
- Purified IgG conjugated to StarBright Violet 440 - liquid
- Product Form
- Purified IgG conjugated to StarBright Violet 515 - liquid
- Product Form
- Purified IgG conjugated to StarBright Violet 610 - liquid
- Reconstitution
- Pack Size: 100 TestsReconstitute with 1 ml distilled waterPack Size: 25 TestsReconstitute with 0.25ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350 - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 25 TestsPrior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Storage
- Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P16284
- Entrez Gene
- PECAM1
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
- Regulatory
- For research purposes only
Applications of CD31 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | Neat Pack Size: 25 µg 1/2 Pack Size: 0.1 mg |
|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/2 Pack Size: 100 Tests Neat Pack Size: 25 Tests |
|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:Pacific Blue® | MCA928PB | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:Alexa Fluor® 700 | MCA928A700 | F | 100 Tests/1ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD31 antibody
-
Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
PLoS One. 7: e35577. -
Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31.
J Immunol. 145 (11): 3889-97. -
DeLisser, H.M. et al. (1994) Molecular and functional aspects of PECAM-1/CD31.
Immunol Today. 15 (10): 490-5. -
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321 (2): 656-62. -
Reedquist, K.A. et al. (2000) The small GTPase, Rap1, mediates CD31-induced integrin adhesion.
J Cell Biol. 148: 1151-8. -
Vernon-Wilson, E.F. et al. (2007) CD31 delays phagocyte membrane repolarization to promote efficient binding of apoptotic cells.
J Leukoc Biol. 82: 1278-88. -
Johnston, A. et al. (2005) The anti-inflammatory action of methotrexate is not mediated by lymphocyte apoptosis, but by the suppression of activation and adhesion molecules.
Clin Immunol. 114: 154-63. -
Hilbe W et al. (2003) Immunohistochemical typing of non-small cell lung cancer on cryostat sections: correlation with clinical parameters and prognosis.
J Clin Pathol. 56 (10): 736-41. -
Stein, A. et al. (2010) Local erythropoietin and endothelial progenitor cells improve regional cardiac function in acute myocardial infarction.
BMC Cardiovasc Disord. Sep; 10:43. -
Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.
Clin Exp Immunol. 130: 256-62. -
Theberge, A.B. et al. (2015) Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis.
Anal Chem. 87 (6): 3239-46. -
Hilbe W et al. (2004) CD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer.
J Clin Pathol. 57 (9): 965-9. -
Yi, T. et al. (2015) Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.
Tissue Eng Part C Methods. 21 (12): 1251-62. -
Palakkan, A.A. et al. (2015) Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells.
Biomed Rep. 3 (5): 626-636. -
Newey SE et al. (2014) The hematopoietic chemokine CXCL12 promotes integration of human endothelial colony forming cell-derived cells into immature vessel networks.
Stem Cells Dev. 23 (22): 2730-43. -
Fabre-Mersseman V et al. (2011) CD4⁺ recent thymic emigrants are infected by HIV in vivo, implication for pathogenesis.
AIDS. 25 (9): 1153-62. -
Patten PE et al. (2008) CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment.
Blood. 111 (10): 5173-81. -
Katz SC et al. (2004) Liver sinusoidal endothelial cells are insufficient to activate T cells.
J Immunol. 173 (1): 230-5. -
Pfisterer K et al. (2015) CD90(+) human dermal stromal cells are potent inducers of FoxP3(+) regulatory T cells.
J Invest Dermatol. 135 (1): 130-41. -
Hale, S.J. et al. (2015) CXCR2 modulates bone marrow vascular repair and haematopoietic recovery post-transplant.
Br J Haematol. 169 (4): 552-64. -
Muthana, M. et al. (2015) Directing cell therapy to anatomic target sites in vivo with magnetic resonance targeting.
Nat Commun. 6: 8009. -
Schuster, C. et al. (2015) Development of Blood and Lymphatic Endothelial Cells in Embryonic and Fetal Human Skin.
Am J Pathol. 185 (9): 2563-74. -
Somers, E. et al. (2016) Vascular Defects and Spinal Cord Hypoxia in Spinal Muscular Atrophy.
Ann Neurol. 79 (2): 217-30. -
Soh, B.S. et al. (2016) Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells.
Nat Commun. 7: 10774. -
GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
PLoS One. 13 (2): e0192244. -
Duque, J.C. et al. (2019) Vascularization of the arteriovenous fistula wall and association with maturation outcomes.
J Vasc Access. : 1129729819863584. [Epub ahead of print]
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
