CD266 antibody | ITEM-4
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F ResetMouse anti Human CD266
- Product Type
- Monoclonal Antibody
- Clone
- ITEM-4
- Isotype
- IgG2b
- Specificity
- CD266
Antibody Quality and Performance Guarantee
| Mouse anti Human CD266 antibody, clone ITEM-4 recognizes human tumor necrosis factor receptor superfamily member 12A, also known as CD266, Fibroblast growth factor-inducible immediate-early response protein 14 or Tweak-receptor. CD266 is a 102 amino acid ~14 kDa type I transmembrane glycoprotein and member of the tumour necrosis factor receptor superfamily (TNFRSF12A) expressed by a variety of tissues, including the heart, lung and kidney, and at elevated levels by human brain and liver tumors. The interaction between CD266 and its ligand CD255 (TWEAK), induces the proliferation and migration of endothelial cells and also angiogenesis, and CD255-induced apoptosis of some tumor cell lines is mediated by CD266 (Nakayama et al. 2003). Mouse anti Human CD266 antibody, clone ITEM-4 is cross-reactive with mouse CD266, and also with Rat-1 fibroblastoid cells in flow cytometry. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human CD266 transfected P815 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the P3U1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control | ||||||
References for CD266 antibody
-
Nakayama, M. et al. (2003) Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death.
J Immunol. 170 (1): 341-8. -
Nakayama, M. et al. (2003) Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies.
Biochem Biophys Res Commun. 306 (4): 819-25. -
Winkles, J.A. and Brown,S. (2010) Targeted Therapy of Fn14-Positive Breast Tumors Using a TWEAK-CytotoxinFusion Protein or Noncovalent Complex.
-
Girgenrath, M. et al. (2006) TWEAK, via its receptor Fn14, is a novel regulator of mesenchymal progenitor cells and skeletal muscle regeneration.
EMBO J. 25: 5826-39.
- Synonyms
- TWEAK-R
- RRID
- AB_10851347
- UniProt
- Q9NP84
- Entrez Gene
- TNFRSF12A
- GO Terms
- GO:0001525 angiogenesis
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
MCA4699GA
0509
MCA4699
0509R
155274
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