CD266 antibody | ITEM-4

Mouse anti Human CD266

Product Type
Monoclonal Antibody
Clone
ITEM-4
Isotype
IgG2b
Specificity
CD266

Product Code Applications Pack Size List Price Your Price Qty
MCA4699GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F WB 0.1 mg loader
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loader
MCA4699
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F WB 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD266 antibody, clone ITEM-4 recognizes human tumor necrosis factor receptor superfamily member 12A, also known as CD266, Fibroblast growth factor-inducible immediate-early response protein 14 or Tweak-receptor. CD266 is a 102 amino acid ~14 kDa type I transmembrane glycoprotein and member of the tumour necrosis factor receptor superfamily (TNFRSF12A) expressed by a variety of tissues, including the heart, lung and kidney, and at elevated levels by human brain and liver tumors.

The interaction between CD266 and its ligand CD255 (TWEAK), induces the proliferation and migration of endothelial cells and also angiogenesis, and CD255-induced apoptosis of some tumor cell lines is mediated by CD266 (Nakayama et al. 2003).

Mouse anti Human CD266 antibody, clone ITEM-4 is cross-reactive with mouse CD266, and also with Rat-1 fibroblastoid cells in flow cytometry.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human CD266 transfected P815 cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the P3U1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2b Negative Control MCA691 F 100 Tests loader
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Description Mouse IgG2b Negative Control

References for CD266 antibody

  1. Nakayama, M. et al. (2003) Fibroblast growth factor-inducible 14 mediates multiple pathways of TWEAK-induced cell death.
    J Immunol. 170 (1): 341-8.
  2. Nakayama, M. et al. (2003) Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies.
    Biochem Biophys Res Commun. 306 (4): 819-25.
  3. Winkles, J.A. and Brown,S. (2010) Targeted Therapy of Fn14-Positive Breast Tumors Using a TWEAK-CytotoxinFusion Protein or Noncovalent Complex.
    U.S. Army Medical Research and Materiel Command, Annual Report
  4. Girgenrath, M. et al. (2006) TWEAK, via its receptor Fn14, is a novel regulator of mesenchymal progenitor cells and skeletal muscle regeneration.
    EMBO J. 25: 5826-39.

Synonyms
TWEAK-R
RRID
AB_10851347
UniProt
Q9NP84
Entrez Gene
TNFRSF12A
GO Terms
GO:0001525 angiogenesis
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0004872 receptor activity
View more products with CD266 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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