CD163 antibody | EDHu-1
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the identification of infiltrating macrophages in the pulmonary tissues of cattle by immunohistochemistry on formalin fixed tissue sections.
Image caption:
Immunohistochemical Labeling of Leukocytes in the Lungs.
Shown are the results of IHC labeling for CD3-like immunoreactivity (CD3-li), CD163-li, IBA-1-li, and IL-17-li in the lung of uninfected control and representative infected Holstein calves. Note: CD3-li, CD163-li, and IL-17-li is significantly increased in the lungs of the infected calf. Furthermore, CD3+, CD163+, IBA-1+ and IL-17+ mononuclear cells are components of vasculitis lesions. Vascular endothelial cells consistently exhibit pronounced IL-17-li in the infected calf, but endothelial IL-17-li is rare in the control calf. Scale bar: 200 μm.
From: Fry LM, Schneider DA, Frevert CW, Nelson DD, Morrison WI, Knowles DP (2016)
East Coast Fever Caused by Theileria parva Is Characterized by Macrophage Activation Associated with Vasculitis and Respiratory Failure.
PLoS ONE 11(5): e0156004.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of perivascular macrophages in human brain by immunofluorescence.
Image caption:
Images demonstrating immunohistological stainings of amylin and double immunofluorescence staining against NG2/amylin, laminin/amylin and CD163/amylin in the hippocampus of the patient with AD and T2D. Amylin cell inclusions are indicated with arrows in (a) and shown in a higher magnification in (b). Pericytes with round cell bodies and NG2-positive coverage of the microvessel surface (green in c), without amylin cell inclusions (red in d), displayed round DAPI-positive cell nuclei (blue in e). The images in (c), (d) and (e) are merged in (f). Cells with more diffuse and weak NG2 staining (indicated by the arrowhead, green in g) and cytosolic amylin cell inclusions (red in h) showed altered cell nuclei (indicated with arrow, blue in i). The adjacent unaffected NG2-positive cell is indicated with an arrowhead in (i). The images in (g), (h) and (i) are merged in (j). Cells enclosed by laminin (green in K) contained amylin grains (red in l) and fragmented DAPI-positive cell nuclei (indicated with an arrow blue in m). The images in (k), (l) and (m) are merged in (n). Loss of NG2 coverage (green in o) was associated with polarized amylin cell inclusion (red in p) and fragmented DAPI-positive cell nuclei (indicated with an arrow, blue in q). The images in (o), (p) and (q) are merged in (r). Staining against macrophage marker CD163 (green in s) did not co-localize with amylin cell inclusions (red in t). The cell nucleus was stained with DAPI (blue in U). The images in (s), (t) and (u) are merged in (v). Scale bars: (a) 50 μm, (b), (c) to (v) 5 μm.
From: Schultz, N. et al. (2016).
Amylin alters human brain pericyte viability and NG2 expression.
J Cereb Blood Flow & Metab. Jun 28 [Epub ahead of print]
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the evaluation of CD163 expression on porcine alveolar macrophages by flow cytometry.
Image caption:
Morphologic and phenotypic characterization of porcine alveolar macrophage cells (PAM) by flow cytometry using SWC3, SWC1, CD163, CD14, CD16, MHCII and DC-sign staining. This figure is a representative of three independent experiments
From: Seeboth J, Solinhac R, Oswald IP, Guzylack-Piriou L.
The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
Vet Res. 2012 Apr 24;43:35.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the evaluation of perivascular macrophages on guinea pig myocardial tissue sections by immunofluorescence on formalin fixed, paraffin embedded tissue sections following heat mediated antigen retrieval in sodium citrate buffer pH 6.0.
Image caption:
Immunofluorescence analysis of myocardial HO-1 and CD163 expression. Surgical non-treated (NT) control animals showed minimal HO-1 (green) expression in resident CD163+ perivascular macrophages (red) (A–C). Sporadic HO-1 expression (white arrow) localized to CD163+ cells observed with Hb alone (D–F). With LPS alone, CD163+ infiltrates and perivascular macrophages showed minimal HO-1 expression (white arrowhead) (G–I). Increased HO-1 expression localized to CD163+ infiltrates and perivascular macrophages observed with LPS + Hb (white arrows) (J–L,M–O). Hp reduces HO-1 expression in CD163+ infiltrates and perivascular cells (P–R,S–U). Nuclei were counterstained with Hoechst 33342 (blue). Scale bar = 10 μm.
From: Baek JH, Zhang X, Williams MC, Schaer DJ, Buehler PW, D'Agnillo F.
Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin.
Toxins (Basel). 2014 Mar 31;6(4):1244-59.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the evaluation of macrophage infiltration in the brains of SIV infected Rhesus macaques by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
Massive activation and productive infection of microglia in CD4-depleted RMs. (a) ISH showing the levels of SIV vRNA+ cells in brain from a representative SIV-uninfected control (top), SIV-infected control (middle) and CD4-depleted SIV-infected (bottom) animal. The amount of SIV vRNA+ cells was markedly higher in CD4-depleted animals than in controls. (b) Immunofluorescence staining for CD163 (left panels), HLA-DR (middle panels) and proliferating cell nuclear antigen (PCNA; right panels) in the parenchyma of one representative SIV-uninfected control (top), one SIV-infected control (middle) and one SIV-infected CD4-depleted (bottom) RM. Nuclei are stained in green; markers of interest in red. (c) Quantitative analyses showing the number of cells per mm2 of tissue that stained positively for the markers of interest. Increased expression of CD163, HLA-DR and PCNA was found in CD4-depleted animals (orange square; n = 4) when compared to both groups of controls (SIV- open circle, n = 3; SIV+ closed circle, n = 3). (d) Single and combined staining for IBA-1 (green), CD163 (blue), and SIV-vRNA (red) in the brain of one representative SIV-infected CD4 depleted RM. The box on the right is a magnification of a microglial cell (IBA-1+) that expresses CD163 and is productively infected (SIV vRNA+).
From: Micci L, Alvarez X, Iriele RI, Ortiz AM, Ryan ES, et al. (2014)
CD4 Depletion in SIV-Infected Macaques Results in Macrophage and Microglia Infection with Rapid Turnover of Infected Cells.
PLoS Pathog 10(10): e1004467.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the identification of intestinal and lymph node macrophages in SIV infected macaques by immunofluorescence on formalin fixed, paraffin embedded tissue sections.
Image caption:
Massive SIV infection of LN and intestinal macrophages in CD4-depleted RMs. (a) Immunofluorescence staining for T cell (CD3; blue) and macrophage (CD68 and/or CD163; green) lineage markers combined with in situ hybridization for SIV vRNA (red) in the LN isolated at day 42 post-infection from one representative undepleted control (left) and one CD4-depleted RM. The vast majority of SIV vRNA+ cells express CD3 in undepleted control but express CD68/CD163 in CD4-depleted RM. (b) The same staining is shown for colon (top) and jejunum (bottom) tissues in two representative CD4-depleted animals. (c) Quantitative image analysis of LN and mucosal tissues showing the fraction of SIV vRNA+ cells that express CD3 or CD68/CD163 in CD4-depleted (n = 12; the 8 animals in this study plus the 4 in Ortiz et al 2011) or undepleted control RMs (n = 6; two SIVmac251 infected animals belonging to a different study were added as controls). (d) Relative abundance of SIV vRNA+ in productively infected T cells and macrophages, determined by measuring the volumetric sum of the SIV vRNA+ intensity integration values in situ from confocal images collected under identical laser settings, is shown in four CD4-depleted RMs. Macrophages on average have higher per cell SIV vRNA+ content compared to CD4+ T cells within the same host. Statistical analyses were determined by Mann-Whitney test.
From: Micci L, Alvarez X, Iriele RI, Ortiz AM, Ryan ES, et al. (2014)
CD4 Depletion in SIV-Infected Macaques Results in Macrophage and Microglia Infection with Rapid Turnover of Infected Cells.
PLoS Pathog 10(10): e1004467.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the detection of CD163 expressing cells in vitro by immunofluorescence.
Image caption:
Expression of Mf-markers and NF-κB subunits in co-cultures of HSC-3 cells and M1 or R848 Mfs. Vybrant CM-Dil labeled HSC-3 cells (red) and unlabeled M1- or R848 Mfs were incubated with DMSO or 10 ng/ml TNF-α for 30 min where after cells were fixed for immunofluorescence with antibodies for CD68, CD163, NF-κB p50 and p65 subunits. Some samples were pre-incubated with 10 μM BAY 11-7082 prior to TNF-α activation. AlexaFluor488-conjugated secondary antibody was used for visualization. Samples were mounted with DAPI- mountain medium to visualize nuclei (blue). Samples were photographed with a Leica Confocal microscope with 63x oil immersion objective. CD163 and NF-κB p65 immunofluorescence from HSC-3 cell and M1 Mf co-cultures (A). CD68 and NF-κB p50 immunofluorescence from HSC-3 cell and R848 Mf co-cultures (B). Red arrows indicate HSC-3 cells and green arrows Mfs. Scale bars 50 μm.
From: Pirilä E, Väyrynen O, Sundquist E, Päkkilä K, Nyberg P, et al. (2015)
Macrophages Modulate Migration and Invasion of Human Tongue Squamous Cell Carcinoma.
PLoS ONE 10(3): e0120895.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the detection of CD163 expressing cells in human tongue carcinoma by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
TAMs localize to evading carcinoma cells in vivo. Human tongue carcinoma tissue sections were subjected to double-staining immunohistochemistry with antibodies for CD163, NF-κB p50 and NF-κB p65. CD163 (red) and NF-κB p50 (grey) staining in A, B. Black arrows show cells with both red and grey color, indicating co-localization of the antibodies. CD163 (red) and NF-κB p65 (grey) staining in C,D, G,H. Blue arrows in H show tongue carcinoma cells. NF-κB p50 (grey) and NF-κB p65 (red) staining in E,F. Black boxes in A, C and G indicates site of magnification in B, D and H respectively. Scale bars in A,C,E and G 200 μm and in B, D, F, and H 50 μm.
From: Pirilä E, Väyrynen O, Sundquist E, Päkkilä K, Nyberg P, et al. (2015)
Macrophages Modulate Migration and Invasion of Human Tongue Squamous Cell Carcinoma.
PLoS ONE 10(3): e0120895.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the detection of CD163 expressing cells in vitro by immunofluorescence.
Image caption:
Vybrant CM-Dil labeled HSC-3 cells (red) and unlabeled M1-, M2 and R848 Mfs were incubated with DMSO where after cells were fixed for immunofluorescence with antibodies for CD68, CD163 and pancytokeratin. AlexaFluor488-conjugated secondary antibody was used for visualization. Samples were mounted with DAPI- mounting medium to visualize nuclei (blue). Samples were photographed with a Leica Confocal microscope with 63x oil immersion objective. CD68 marker staining in DMSO-treated M1 Mfs (A), M2 Mfs (B) and R848 Mfs (C). CD163 marker staining in DMSO-treated M2 Mfs treated (D) and R848 Mfs (E). DMSO-treated HSC-3 cells (red) stained with pancytokeratin (green) in F. Scale bars 50 μm.
From: Pirilä E, Väyrynen O, Sundquist E, Päkkilä K, Nyberg P, et al. (2015)
Macrophages Modulate Migration and Invasion of Human Tongue Squamous Cell Carcinoma.
PLoS ONE 10(3): e0120895.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the identification and enumeration of infiltrating macrophages in both polyploid and non-polyploid colorectal adenoma tissue sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical assessment of stromal immune cell infiltrates in polypoid and nonpolypoid colorectal adenomas.
CD4+, FOXP3+, CD8+, CD68+, CD163+, and MHC-I+ immune cell densities (i.e., number of positively-stained cells per unit area; see Methods) in the stroma of polypoid adenomas were consistently higher than those observed in nonpolypoid lesions. Intraepithelial CD8+ (cytotoxic) T cells are also seen in the epithelial compartments of both types of tumor. In calculating CD68+ and CD163+ macrophage densities, we counted only nuclei surrounded by CD68+ or CD163+ granulations and excluded elongated or fragmented cells with granular cytoplasmic positivity. MHC-I labelling (granular, compact, or membranous) was observed in a variety of stromal cells. MHC-I+ epithelial cells were also present in both types of tumor, but their presence was not quantified in this study. Magnification for all panels: 400X.
From: Maglietta A, Maglietta R, Staiano T, Bertoni R, Ancona N, Marra G, et al. (2016)
The Immune Landscapes of Polypoid and Nonpolypoid Precancerous Colorectal Lesions.
PLoS ONE 11(7): e0159373.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody clone EDHu-1 (MCA1853) used for the identification of skin macrophages by immunohistochemistry.
Image caption:
EBV-lytic proteins are expressed in dcSSc circulating monocytes and dcSSc skin macrophages. Immunohistochemistry of skin sections, representative immunohistologic images of CD163+ macrophages (brown) and EBV/lytic protein Zebra + (blue) cells in skin sections from dcSSc and HD. Original magnification 2× (upper panels) and 10× (inserts and lower panels). The arrows indicate monocytes represented in the high magnification inserts. Scale bars = 5μm
From: Farina A, Peruzzi G, Lacconi V, Lenna S, Quarta S, Rosato E, Vestri AR, York M, Dreyfus DH, Faggioni A, Morrone S, Trojanowska M, Farina GA.
Epstein-Barr virus lytic infection promotes activation of Toll-like receptor 8 innate immune response in systemic sclerosis monocytes.
Arthritis Res Ther. 2017 Feb 28;19(1):39.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used for the demonstration of CD163 expression on liver macrophages from patients with primary sclerosing cholangitis by immunohistochemistry on formalin fixed OCT-embedded cryosections.
Image caption:
T cells infiltrate PSC-patient livers. Representative immunostaining and quantification analysis of (A) CD3, (B) TCR-Vα7.2, (C) NKp46, (D) CD163, and (E) MHC class I expression in controls (n = 14–17) and PSC patients (n = 14–17). Samples were counterstained with hematoxylin and dark brown indicate immunoreaction product. Quantification was performed to calculate mean intensity of the stainings (ACIA) and frequency of expression (percentage of stained area out of total area).
From: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, et al. (2014)
In Situ Characterization of Intrahepatic Non-Parenchymal Cells in PSC Reveals Phenotypic Patterns Associated with Disease Severity.
PLoS ONE 9(8): e105375.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label macrophages in human liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative immunostaining of CD163 and CD68 on consecutive sections of paired tumor tissue and peritumoral liver tissue.
The densities of intratumoral CD68+ cells (A) and CD163+ cells (C) were lower in tumor than in corresponding peritumoral liver tissue (B and D). The density of CD163+ cells was higher than that of CD68+ cells in both tumor and in peritumoral liver tissue. Scale bar, 200 μm.
From: Kong L-Q, Zhu X-D, Xu H-X, Zhang J-B, Lu L, Wang W-Q, et al. (2013)
The Clinical Significance of the CD163+ and CD68+ Macrophages in Patients with Hepatocellular Carcinoma.
PLoS ONE 8(3): e59771. doi: 10.1371/journal.pone.0059771
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to visualize M2 macrophages in human liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Expression of CD163 in liver sections, serum concentration of sCD163 and proportion of CD14 in controls and patients with CHC with different severities of fibrosis (F<2 or F≥2). (A-C) Representative histology images of liver sections with (A) H&E, (B) Masson-trichrome and (C) CD163 immunohistochemical staining (original magnification, x200). In CHC liver tissues, enhanced expression of CD163 was localized in the fibrous septum.
From: Zhao, S., Li, W., Fu, N., Kong, L., Zhang, Q., Han, F., Ren, W., Cui, P., Du, J., Wang, B., Zhang, Y., Wang, R., Kong, L., Nan, Y.
"CD14+ monocytes and CD163+ macrophages correlate with the severity of liver fibrosis in patients with chronic hepatitis C".
Experimental and Therapeutic Medicine 20.6 (2020): 228.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label macrophages in Cynomolgus monkey endometrium by immunohistochemistry on formalin fixed , paraffin embedded tissue sections.
Image caption:
Histology of endometriosis from an advanced lesion and an early lesion in cynomolgus monkeys.
For an early lesion, a tissue was taken from a brown deposition on the peritoneum from the vesicouterine pouch of monkey #7 that died of acute gastrectasia unrelated to endometriosis two days after the follow-up laparoscopy performed 11 months after the initial laparoscopy (G-L).(G) Inflammation and an endometrial gland were seen in the peritoneum of the tissue (× 40). (H) Higher magnification of G shows infiltration of mononuclear cells (× 400). (I) Higher magnification of G shows an endometrial gland (× 400). (J) CD3-positive lymphocytes (T cells) seen in the same location of H in a serial section (× 400). (K) CD163-positive macrophages and macrophage-derived foreign body multinucleated giant cells (arrow) seen in the same location of H in a serial section (× 400). (L) Expression of estrogen receptor alpha of an endometrial gland seen in the same location of I in a serial section (× 400). G, H, and I (H & E staining); J, K, and L (Immunohistochemistry).
From: Hayashi K, Nakayama M, Iwatani C, Tsuchiya H, Nakamura S, Nonoguchi K, Itoh Y, Tsuji S, Ishigaki H, Mori T, Murakami T, Ogasawara K.
The Natural History of Spontaneously Occurred Endometriosis in Cynomolgus Monkeys by Monthly Follow-Up Laparoscopy for Two Years.
Tohoku J Exp Med. 2020 Aug;251(4):241-53.
doi: 10.1620/tjem.251.241.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 used for demonstrating CD163 expression by anthracosis associated myeloid cells by immunohistochemistry.
Image caption:
Tumor-promoting phenotype of the myeloid clusters. IHC staining demonstrating the expression of CD163, IL-6, IL-10, VEGF-A, MMP-9, SDF-1 and Bcl-xL by the myeloid cells associated with anthracosis. For each protein, representative images showing positive and negative staining areas were selected from the same slide. The quantification was performed by analyzing random images of myeloid cluster areas associated with anthracosis and other areas (10 images for each group) from 10 patients. Two-tailed Student's t-test was used for statistical analysis. Shown are means ± SEM, *** P<0.001. (Scale bar, 200 μm for 100× and 400×; 25 μm for 1600×)
From: Zhang W, Pal SK, Liu X, Yang C, Allahabadi S, et al. (2013)
Myeloid Clusters Are Associated with a Pro-Metastatic Environment and Poor Prognosis in Smoking-Related Early Stage Non-Small Cell Lung Cancer.
PLoS ONE 8(5): e65121.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody (MCA1853) used for the evaluation of CD163 expression on microglia by immunohistochemistry on formalin fixed, paraffin embedded brain sections.
Image caption:
Rasmussen's encephalitis. (A) Areas of active encephalitis and cortical scarring highlighted with increased numbers of HLADR-positive microglia/macrophages, as well as interstitial rod-like CD163-positive microglia cells (inset). (B) On adjacent sections, labelling with pS6 235/236 showed a similar distribution of cellular labelling as well as with pS6 240/244 (C). (D) pS6 240/244 highlighted many of the enlarged dysmorphic neurones in RE cases, as confirmed with co-labelling with neurofilaments (inset). (E) pS6 236/26 demonstrated labelling of smaller glial cells as well as (F) bipolar rod shape cells. Double labelling studies in RE cases confirmed the majority of pS6 labelled cells were not GFAP-positive astroglia (G) but co-labelled with populations of iba1-positive cells (microglial marker) (H), nestin and doublecortin (inset) (I) positive cells. Bar in A, B, C equivalent to approximately 100 microns, in d approximately 50 microns and E, F, G, H, I approximately 30 microns.
From: Liu J, Reeves C, Michalak Z, Coppola A, Diehl B, Sisodiya SM, Thom M.
Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
Acta Neuropathol Commun. 2014 Jul 8;2:71.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to evaluate pCD163 expression in transfected PK-15 cells by western blotting.
Image caption:
Identification of residues in pCD163 SRCR5 important for PRRSV-2 infection. A RT-qPCR analyses of the effect of mutated pCD163 on PRRSV-2 replication. WT or mutant pCD163 constructs (3 μg/well, 6-well plate) were transfected into PK-15 cells. After 24 h, the transfected PK-15 cells were inoculated with PRRSV-2 strain BJ-4 at a MOI of 1. At 12 hpi, total RNAs of infected PK-15 cells were extracted and then the viral RNA was measured by RT-qPCR. In parallel, expression levels of WT and mutant pCD163 were tested by IB using a commercial mouse anti-human CD163 antibody (MCA1853). Data represent means ± SEM from three independent experiments. *p <0.05.
From: Ma H, Li R, Jiang L, Qiao S, Chen XX, Wang A, Zhang G.
Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro.
Vet Res. 2021 Jun 30;52(1):97.
doi: 10.1186/s13567-021-00969-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to evaluate pCD163 expression in transfected PK-15 cells by western blotting.
Image caption:
Identification of residues in pCD163 SRCR5 important for PRRSV-2 infection.B Analyses of PRRSV N protein expression in WT or mutant pCD163-expressed cells. The infected WT or mutant pCD163-expressed cells were harvested and lysed at 24 hpi. PRRSV N protein in WT or mutant pCD163-expressed cells was measured by IB. Significant reduction in PRRSV N protein expression is marked by an arrow. In parallel, expression levels of WT and mutant pCD163 were tested by IB using a commercial mouse anti-human CD163 antibody (MCA1853).
From: Ma H, Li R, Jiang L, Qiao S, Chen XX, Wang A, Zhang G.
Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro.
Vet Res. 2021 Jun 30;52(1):97.
doi: 10.1186/s13567-021-00969-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to evaluate pCD163 expression in transfected PK-15 cells by western blotting.
Image caption:
Analyses of simultaneous mutagenesis of the identified two residues for PRRSV-2 infection. A TCID50 analyses of the effect of mutated CD163 on PRRSV-2 viral titers. PK-15 cells were transfected with WT or mutated pCD163 constructs. After 24 h, the transfected PK-15 cells were inoculated with PRRSV strain BJ-4 or HN07-1 at a MOI of 1 for 48 h. The viral yields were measured by TCID50 assay in MARC-145 cells. Data represent means ± SEM of three independent experiments. **p <0.01, ***p <0.001 for mutant CD163 compared to the WT one. #p <0.05 for simultaneous mutagenesis at position 534 and 561 of CD163 compared to the single-site mutagenesis. B Analyses of PRRSV N protein expression in WT or mutant pCD163-expressed cells. PRRSV N protein expression was tested by IB as described in Figure 5B.
From: Ma H, Li R, Jiang L, Qiao S, Chen XX, Wang A, Zhang G.
Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro.
Vet Res. 2021 Jun 30;52(1):97.
doi: 10.1186/s13567-021-00969-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label M2 type macrophages in high-grade glioma tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemistry. Representative microscopic images (200x magnification, scale bar 100 μm) of one GBM patient including the HE (first column) and the IHC staining for CD45 (second column), CD68 (third column) and CD163 (fourth column) Images demonstrate the morphological heterogeneity within the tumor tissue (A), the tumor core (B), the infiltration zone (C) and the perivascular area (D).
From: Kemmerer CL, Schittenhelm J, Dubois E, Neumann L, Häsler LM, Lambert M, Renovanz M, Kaeser SA, Tabatabai G, Ziemann U, Naumann U, Kowarik MC.
Cerebrospinal fluid cytokine levels are associated with macrophage infiltration into tumor tissues of glioma patients.
BMC Cancer. 2021 Oct 15;21(1):1108.
doi: 10.1186/s12885-021-08825-1.
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Mouse anti Human CD163 antibody, clone EDHu-1 (MCA1853) used to label CD163 expressing cells in ovine tissues by immunohistochemistry of formalin fixed, paraffin embedded tissue sections following proteinase K mediated antigen retrieval.
Image caption:
Representative images of sections of the native ovine ACL and intra-articular graft portion stained with antibodies to CD3, Ki-67, MAC 387; CD163 and CTGF.
A-E Native ovine ACL; F-J decellularised pSFT explanted at 4 weeks; K–O; decellularised pSFT explanted at 12 weeks; P-T decellularised pSFT explanted at 26 weeks; U–Y ovine SFT (allograft control) explanted at 26 weeks. Images captured at 10× magnification unless otherwise stated. Scale bars show 100 μm.
From: Edwards JH, Jones GL, Herbert A, Fisher J, Ingham E.
Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.
Biomaterials. 2021 Oct 21;279:121204.
doi: 10.1016/j.biomaterials.2021.121204
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD163:Alexa Fluor® 488
Mouse anti Human CD163:FITC
Mouse anti Human CD163
Mouse anti Human CD163:RPE
- Product Type
- Monoclonal Antibody
- Clone
- EDHu-1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1853A488 | F | 100 Tests/1ml |
|
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| MCA1853F | F | 0.1 mg |
|
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| MCA1853 | C E F IF IY P WB | 0.2 mg |
|
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| MCA1853T | C E F IF IY P WB | 25 µg |
|
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| MCA1853PE | F | 100 Tests |
|
Mouse anti Human CD163 antibody, clone EDHu-1 recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 expression may be induced on monocytes by culture in dexamethasone.
Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsen et al. 2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment.
Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsen et al. 2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Sheep Pig Guinea Pig Bovine Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q86VB7
- Entrez Gene
- CD163
- GO Terms
- GO:0005515 protein binding
- GO:0006953 acute-phase response
- GO:0005576 extracellular region
- GO:0005044 scavenger receptor activity
- GO:0005887 integral to plasma membrane
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD163 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| ELISA | |||
| Flow Cytometry | 1/10 | 1/50 | |
| Immunoassay | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting | |||
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
|
|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for CD163 antibody
-
Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor.
Nature. 409 (6817): 198-201. -
Madsen, M. et al. (2004) Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region.
J Biol Chem. 279 (49): 51561-7. -
Kim, W.K. et al. (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood.
Am J Pathol. 168 (3): 822-34. -
Moreno JA et al. (2010) Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
Arterioscler Thromb Vasc Biol. 30 (6): 1253-62. -
Herrmann-Hoesing, L.M. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep.
Vet Pathol. 47: 518-28. -
Asleh, R. et al. (2003) Genetically determined heterogeneity in hemoglobin scavenging and susceptibility to diabetic cardiovascular disease.
Circ Res. 92: 1193-200. -
Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
Blood 109: 5223-9. -
Jensen, T.O. et al. (2009) Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma.
J Clin Oncol. 27: 3330-7. -
Montes de Oca, M. et al. (2005) Skeletal muscle inflammation and nitric oxide in patients with COPD.
Eur Respir J. 26: 390-7. -
Martens JH et al. (2006) Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis.
J Pathol. 208 (4): 574-89. -
Vinet-Oliphant, H. et al. (2010) Neurokinin-1 receptor (NK1-R) expression in the brains of SIV-infected rhesus macaques: implications for substance P in NK1-R immune cell trafficking into the CNS.
Am J Pathol. 177: 1286-97. -
Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
J Transl Med. 4: 30. -
Grund, S. et al (2009) The microglial/macrophagic response at the tumour-brain border of invasive meningiomas.
Neuropathol Appl Neurobiol. 35: 82-8. -
Jorgensen, J.M. et al (2009) Expression level, tissue distribution pattern, and prognostic impact of vascular endothelial growth factors VEGF and VEGF-C and their receptors Flt-1, KDR, and Flt-4 in different subtypes of non-Hodgkin lymphomas.
Leuk Lymphoma. 50: 1647-60. -
Moreno, J.A. et al. (2009) The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.
Atherosclerosis. 207: 103-10. -
Tang, Z. et al. (2013) Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells.
Endocrinology 154: 471-82. -
Boyle, J.J. et al. (2009) Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.
Am J Pathol. 174: 1097-108. -
Taus, N.S. et al. (2010) Sheep (Ovis aries) airway epithelial cells support ovine herpesvirus 2 lytic replication in vivo.
Vet Microbiol. 145: 47-53. -
Seeboth, J. et al. (2012) The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
Vet Res. 43: 35. -
Berglin, L. et al. (2014) In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
PLoS One 9: e105375. -
Liu, J. et al. (2014) Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
Acta Neuropathol Commun. 2: 71. -
Baek, J.H. et al. (2014) Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin.
Toxins (Basel) 6: 1244-59. -
Micci, L, et al. (2014) CD4 depletion in SIV-infected macaques results in macrophage and microglia infection with rapid turnover of infected cells.
PLoS Pathog. 10: e1004467. -
Pirilä E et al. (2015) Macrophages modulate migration and invasion of human tongue squamous cell carcinoma.
PLoS One 10 (3): e0120895. -
Arranz-Solís D et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 47 (1): 2. -
Lakritz, J R. et al. (2016) α4-Integrin Antibody Treatment Blocks Monocyte/Macrophage Traffic to, Vascular Cell Adhesion Molecule-1 Expression in, and Pathology of the Dorsal Root Ganglia in an SIV Macaque Model of HIV-Peripheral Neuropathy.
Am J Pathol. 186 (7): 1754-61. -
Fry, L.M. et al. (2016) East Coast Fever Caused by Theileria parva Is Characterized by Macrophage Activation Associated with Vasculitis and Respiratory Failure.
PLoS One 11 (5): e0156004. -
Schultz, N. et al. (2017) Amylin alters human brain pericyte viability and NG2 expression.
J Cereb Blood Flow Metab. 37 (4): 1470-82. -
Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
PLoS One 8: e65121. -
Furukawa S et al. (2017) Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease.
Sci Rep. 7: 42413. -
Farina, A. et al. (2017) Epstein-Barr virus lytic infection promotes activation of Toll-like receptor 8 innate immune response in systemic sclerosis monocytes.
Arthritis Res Ther. 19 (1): 39. -
Mallard, J. et al. (2017) A method for obtaining simian immunodeficiency virus RNA sequences from laser capture microdissected and immune captured CD68+ and CD163+ macrophages from frozen tissue sections of bone marrow and brain.
J Immunol Methods. 442: 59-63. -
Blair, T.C. et al. (2016) Immunopathology of Japanese macaque encephalomyelitis is similar to multiple sclerosis.
J Neuroimmunol. 291: 1-10. -
Zhu, C. et al. (2017) Activation of CECR1 in M2-like TAMs promotes paracrine stimulation-mediated glial tumor progression.
Neuro Oncol. 19 (5): 648-59. -
Derricott, H. et al. (2016) Characterizing Villitis of Unknown Etiology and Inflammation in Stillbirth.
Am J Pathol. 186 (4): 952-61. -
Wächter, C. et al. (2016) Loss of cerebellar neurons in the progression of lentiviral disease: effects of CNS-permeant antiretroviral therapy.
J Neuroinflammation. 13 (1): 272. -
Chen, J. et al. (2019) Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163.
Int J Biol Sci. 15 (2): 481-492. -
Palaiologou, E. et al. (2020) Human placental villi contain stromal macrovesicles associated with networks of stellate cells.
J Anat. 236 (1): 132-41. -
Kong, L.Q. et al. (2013) The clinical significance of the CD163+ and CD68+ macrophages in patients with hepatocellular carcinoma.
PLoS One. 8 (3): e59771. -
Zhao, S. et al. (2020) CD14+ monocytes and CD163+ macrophages correlate with the severity of liver fibrosis in patients with chronic hepatitis C
Experimental and Therapeutic Medicine. 20 (6): 1-1. -
Hayashi, K. et al. (2020) The Natural History of Spontaneously Occurred Endometriosis in Cynomolgus Monkeys by Monthly Follow-Up Laparoscopy for Two Years.
Tohoku J Exp Med. 251 (4): 241-53. -
Gonçalves, V.M. et al. (2021) Macrophage and Lymphocyte Infiltration Is Associated with Volumetric Tumor Size but Not with Volumetric Growth in the T&yyml;bingen Schwannoma Cohort.
Cancers (Basel). 13 (3): 466. -
Pestronk, A. (2020) Chronic Graft Versus Host Myopathies: Noninflammatory, Multi-Tissue Pathology With Glycosylation Disorders.
J Neuropathol Exp Neurol. 79 (1): 102-12. -
Holsapple, J.S. et al. (2021) Low Intensity Shockwave Treatment Modulates Macrophage Functions Beneficial to Healing Chronic Wounds.
Int J Mol Sci. 22(15):7844. -
da Silva M.C.M. et al. (2021) Inhibition of CSF1R, a receptor involved in microglia viability, alters behavioral and molecular changes induced by cocaine.
Sci Rep. 11 (1): 15989. -
Frafjord, A. et al. (2020) Antibody combinations for optimized staining of macrophages in human lung tumours.
Scand J Immunol. 92 (1): e12889. -
Ma, H. et al. (2021) Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro..
Vet Res. 52 (1): 97. -
Kemmerer, C.L. et al. (2021) Cerebrospinal fluid cytokine levels are associated with macrophage infiltration into tumor tissues of glioma patients
BMC Cancer. 21(1):1108. -
Lehmann, M. et al. (2021) New onset of mainly guttate psoriasis after COVID-19 vaccination: a case report.
J Eur Acad Dermatol Venereol. 35 (11): e752-e755. -
Baumann, D. et al. (2021) p38 MAPK signaling in M1 macrophages results in selective elimination of M2 macrophages by MEK inhibition.
J Immunother Cancer.9 (7): e002319. -
Ikezumi, Y. et al. (2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.
Pediatr Nephrol. 36 (2): 349-59. -
Eligini, S. et al. (2019) Biological profile of monocyte-derived macrophages in coronary heart disease patients: implications for plaque morphology.
Sci Rep. 9 (1): 8680. -
Edwards, J.H. et al. (2021) Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.
Biomaterials. 279: 121204.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Our CD163 (EDHu-1) Antibody has been referenced in >88 publications*
*Based on June 2020 data from CiteAb's antibody search engine.
