CD163 antibody | EDHu-1

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Mouse anti Human CD163:Alexa Fluor® 488

Mouse anti Human CD163:FITC

Mouse anti Human CD163

Mouse anti Human CD163:RPE

Product Type
Monoclonal Antibody
Clone
EDHu-1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1853A488 F 100 Tests/1ml
MCA1853F F 0.1 mg
MCA1853T C E F IF IY P WB 25 µg
MCA1853 C E F IF IY P WB 0.2 mg
MCA1853PE F 100 Tests
Mouse anti Human CD163 antibody, clone EDHu-1 recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes but expression may be induced on monocytes by culture in dexamethasone.

Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsen et al. 2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Sheep
Pig
Guinea Pig
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
Q86VB7 Related reagents
Entrez Gene
CD163 Related reagents
GO Terms
GO:0005515 protein binding
GO:0006953 acute-phase response
GO:0005576 extracellular region
GO:0005044 scavenger receptor activity
GO:0005887 integral to plasma membrane
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD163 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat 1/10
ELISA
Flow Cytometry 1/10 1/50
Immunoassay
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 100 Tests/1ml F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Immunoassay

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for CD163 antibody

  1. Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor.
    Nature. 409 (6817): 198-201.
  2. Madsen, M. et al. (2004) Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region.
    J Biol Chem. 279 (49): 51561-7.
  3. Kim, W.K. et al. (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood.
    Am J Pathol. 168 (3): 822-34.
  4. Moreno JA et al. (2010) Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
    Arterioscler Thromb Vasc Biol. 30 (6): 1253-62.
  5. Herrmann-Hoesing, L.M. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep.
    Vet Pathol. 47: 518-28.
  6. Asleh, R. et al. (2003) Genetically determined heterogeneity in hemoglobin scavenging and susceptibility to diabetic cardiovascular disease.
    Circ Res. 92: 1193-200.
  7. Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
    Blood 109: 5223-9.
  8. Jensen, T.O. et al. (2009) Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma.
    J Clin Oncol. 27: 3330-7.
  9. Montes de Oca, M. et al. (2005) Skeletal muscle inflammation and nitric oxide in patients with COPD.
    Eur Respir J. 26: 390-7.
  10. Martens JH et al. (2006) Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis.
    J Pathol. 208 (4): 574-89.
  11. Vinet-Oliphant, H. et al. (2010) Neurokinin-1 receptor (NK1-R) expression in the brains of SIV-infected rhesus macaques: implications for substance P in NK1-R immune cell trafficking into the CNS.
    Am J Pathol. 177: 1286-97.
  12. Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
    J Transl Med. 4: 30.
  13. Grund, S. et al (2009) The microglial/macrophagic response at the tumour-brain border of invasive meningiomas.
    Neuropathol Appl Neurobiol. 35: 82-8.
  14. Jorgensen, J.M. et al (2009) Expression level, tissue distribution pattern, and prognostic impact of vascular endothelial growth factors VEGF and VEGF-C and their receptors Flt-1, KDR, and Flt-4 in different subtypes of non-Hodgkin lymphomas.
    Leuk Lymphoma. 50: 1647-60.
  15. Moreno, J.A. et al. (2009) The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.
    Atherosclerosis. 207: 103-10.
  16. Tang, Z. et al. (2013) Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells.
    Endocrinology 154: 471-82.
  17. Boyle, J.J. et al. (2009) Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.
    Am J Pathol. 174: 1097-108.
  18. Taus, N.S. et al. (2010) Sheep (Ovis aries) airway epithelial cells support ovine herpesvirus 2 lytic replication in vivo.
    Vet Microbiol. 145: 47-53.
  19. Seeboth, J. et al. (2012) The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
    Vet Res. 43: 35.
  20. Berglin, L. et al. (2014) In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
    PLoS One 9: e105375.
  21. Liu, J. et al. (2014) Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
    Acta Neuropathol Commun. 2: 71.
  22. Baek, J.H. et al. (2014) Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin.
    Toxins (Basel) 6: 1244-59.
  23. Micci, L, et al. (2014) CD4 depletion in SIV-infected macaques results in macrophage and microglia infection with rapid turnover of infected cells.
    PLoS Pathog. 10: e1004467.
  24. Pirilä E et al. (2015) Macrophages modulate migration and invasion of human tongue squamous cell carcinoma.
    PLoS One 10 (3): e0120895.
  25. Arranz-Solís D et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
    Vet Res. 47 (1): 2.
  26. Lakritz, J R. et al. (2016) α4-Integrin Antibody Treatment Blocks Monocyte/Macrophage Traffic to, Vascular Cell Adhesion Molecule-1 Expression in, and Pathology of the Dorsal Root Ganglia in an SIV Macaque Model of HIV-Peripheral Neuropathy.
    Am J Pathology May 6 [Epub ahead of print]
  27. Fry, L.M. et al. (2016) East Coast Fever Caused by Theileria parva Is Characterized by Macrophage Activation Associated with Vasculitis and Respiratory Failure.
    PLoS One 11 (5): e0156004.
  28. Schultz, N. et al. (2016) Amylin alters human brain pericyte viability and NG2 expression.
    J Cereb Blood Flow Metab. Jun 28 [Epub ahead of print]
  29. Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
    PLoS One 8: e65121.
  30. Furukawa S et al. (2017) Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease.
    Sci Rep. 7: 42413.
  31. Farina, A. et al. (2017) Epstein-Barr virus lytic infection promotes activation of Toll-like receptor 8 innate immune response in systemic sclerosis monocytes.
    Arthritis Res Ther. 19 (1): 39.
  32. Mallard, J. et al. (2017) A method for obtaining simian immunodeficiency virus RNA sequences from laser capture microdissected and immune captured CD68+ and CD163+ macrophages from frozen tissue sections of bone marrow and brain.
    J Immunol Methods pii: S0022-1759(16)30299-X. [Epub ahead of print]
  33. Blair, T.C. et al. (2016) Immunopathology of Japanese macaque encephalomyelitis is similar to multiple sclerosis.
    J Neuroimmunol. 291: 1-10.
  34. Zhu, C. et al. (2017) Activation of CECR1 in M2-like TAMs promotes paracrine stimulation-mediated glial tumor progression.
    Neuro Oncol. Jan 3. pii: now251. [Epub ahead of print]
  35. Derricott, H. et al. (2016) Characterizing Villitis of Unknown Etiology and Inflammation in Stillbirth.
    Am J Pathol. 186 (4): 952-61.
  36. Wächter, C. et al. (2016) Loss of cerebellar neurons in the progression of lentiviral disease: effects of CNS-permeant antiretroviral therapy.
    J Neuroinflammation. 13 (1): 272.
CiteAb logo - trusted, tested, published

Our CD163 (EDHu-1) Antibody has been referenced in >60 publications*


*Based on February 2018 data from CiteAb's antibody search engine.