CD163 antibody | EDHu-1

Mouse anti Human CD163

Product Type
Monoclonal Antibody
Clone
EDHu-1
Isotype
IgG1
Specificity
CD163

Product Code Applications Pack Size List Price Your Price Qty
MCA1853
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF IY P WB 0.2 mg loader
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loader
MCA1853T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E F IF IY P WB 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD163 antibody, clone EDHu-1 recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 expression may be induced on monocytes by culture in dexamethasone.

Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsen et al. 2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment.
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Our CD163 (EDHu-1) Antibody has been referenced in >88 publications*


*Based on June 2020 data from CiteAb's antibody search engine.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Sheep
Pig
Guinea Pig
Bovine
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1/10 1/50
Immunoassay
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD163 antibody

  1. Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor.
    Nature. 409 (6817): 198-201.
  2. Madsen, M. et al. (2004) Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region.
    J Biol Chem. 279 (49): 51561-7.
  3. Kim, W.K. et al. (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood.
    Am J Pathol. 168 (3): 822-34.
  4. Moreno JA et al. (2010) Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
    Arterioscler Thromb Vasc Biol. 30 (6): 1253-62.
  5. Herrmann-Hoesing, L.M. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep.
    Vet Pathol. 47: 518-28.
  6. Asleh, R. et al. (2003) Genetically determined heterogeneity in hemoglobin scavenging and susceptibility to diabetic cardiovascular disease.
    Circ Res. 92: 1193-200.
  7. Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
    Blood 109: 5223-9.
  8. Jensen, T.O. et al. (2009) Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma.
    J Clin Oncol. 27: 3330-7.
  9. View The Latest Product References
  10. Montes de Oca, M. et al. (2005) Skeletal muscle inflammation and nitric oxide in patients with COPD.
    Eur Respir J. 26: 390-7.
  11. Martens JH et al. (2006) Differential expression of a gene signature for scavenger/lectin receptors by endothelial cells and macrophages in human lymph node sinuses, the primary sites of regional metastasis.
    J Pathol. 208 (4): 574-89.
  12. Vinet-Oliphant, H. et al. (2010) Neurokinin-1 receptor (NK1-R) expression in the brains of SIV-infected rhesus macaques: implications for substance P in NK1-R immune cell trafficking into the CNS.
    Am J Pathol. 177: 1286-97.
  13. Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
    J Transl Med. 4: 30.
  14. Grund, S. et al (2009) The microglial/macrophagic response at the tumour-brain border of invasive meningiomas.
    Neuropathol Appl Neurobiol. 35: 82-8.
  15. Jorgensen, J.M. et al (2009) Expression level, tissue distribution pattern, and prognostic impact of vascular endothelial growth factors VEGF and VEGF-C and their receptors Flt-1, KDR, and Flt-4 in different subtypes of non-Hodgkin lymphomas.
    Leuk Lymphoma. 50: 1647-60.
  16. Moreno, J.A. et al. (2009) The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.
    Atherosclerosis. 207: 103-10.
  17. Tang, Z. et al. (2013) Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells.
    Endocrinology 154: 471-82.
  18. Boyle, J.J. et al. (2009) Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.
    Am J Pathol. 174: 1097-108.
  19. Taus, N.S. et al. (2010) Sheep (Ovis aries) airway epithelial cells support ovine herpesvirus 2 lytic replication in vivo.
    Vet Microbiol. 145: 47-53.
  20. Seeboth, J. et al. (2012) The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
    Vet Res. 43: 35.
  21. Berglin, L. et al. (2014) In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
    PLoS One 9: e105375.
  22. Liu, J. et al. (2014) Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
    Acta Neuropathol Commun. 2: 71.
  23. Baek, J.H. et al. (2014) Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin.
    Toxins (Basel) 6: 1244-59.
  24. Micci, L, et al. (2014) CD4 depletion in SIV-infected macaques results in macrophage and microglia infection with rapid turnover of infected cells.
    PLoS Pathog. 10: e1004467.
  25. Pirilä E et al. (2015) Macrophages modulate migration and invasion of human tongue squamous cell carcinoma.
    PLoS One 10 (3): e0120895.
  26. Arranz-Solís D et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
    Vet Res. 47 (1): 2.
  27. Lakritz, J R. et al. (2016) α4-Integrin Antibody Treatment Blocks Monocyte/Macrophage Traffic to, Vascular Cell Adhesion Molecule-1 Expression in, and Pathology of the Dorsal Root Ganglia in an SIV Macaque Model of HIV-Peripheral Neuropathy.
    Am J Pathol. 186 (7): 1754-61.
  28. Fry, L.M. et al. (2016) East Coast Fever Caused by Theileria parva Is Characterized by Macrophage Activation Associated with Vasculitis and Respiratory Failure.
    PLoS One 11 (5): e0156004.
  29. Schultz, N. et al. (2017) Amylin alters human brain pericyte viability and NG2 expression.
    J Cereb Blood Flow Metab. 37 (4): 1470-82.
  30. Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
    PLoS One 8: e65121.
  31. Furukawa S et al. (2017) Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease.
    Sci Rep. 7: 42413.
  32. Farina, A. et al. (2017) Epstein-Barr virus lytic infection promotes activation of Toll-like receptor 8 innate immune response in systemic sclerosis monocytes.
    Arthritis Res Ther. 19 (1): 39.
  33. Mallard, J. et al. (2017) A method for obtaining simian immunodeficiency virus RNA sequences from laser capture microdissected and immune captured CD68+ and CD163+ macrophages from frozen tissue sections of bone marrow and brain.
    J Immunol Methods. 442: 59-63.
  34. Blair, T.C. et al. (2016) Immunopathology of Japanese macaque encephalomyelitis is similar to multiple sclerosis.
    J Neuroimmunol. 291: 1-10.
  35. Zhu, C. et al. (2017) Activation of CECR1 in M2-like TAMs promotes paracrine stimulation-mediated glial tumor progression.
    Neuro Oncol. 19 (5): 648-59.
  36. Derricott, H. et al. (2016) Characterizing Villitis of Unknown Etiology and Inflammation in Stillbirth.
    Am J Pathol. 186 (4): 952-61.
  37. Wächter, C. et al. (2016) Loss of cerebellar neurons in the progression of lentiviral disease: effects of CNS-permeant antiretroviral therapy.
    J Neuroinflammation. 13 (1): 272.
  38. Chen, J. et al. (2019) Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163.
    Int J Biol Sci. 15 (2): 481-492.
  39. Palaiologou, E. et al. (2020) Human placental villi contain stromal macrovesicles associated with networks of stellate cells.
    J Anat. 236 (1): 132-41.
  40. Kong, L.Q. et al. (2013) The clinical significance of the CD163+ and CD68+ macrophages in patients with hepatocellular carcinoma.
    PLoS One. 8 (3): e59771.
  41. Zhao, S. et al. (2020) CD14+ monocytes and CD163+ macrophages correlate with the severity of liver fibrosis in patients with chronic hepatitis C
    Experimental and Therapeutic Medicine. 20 (6): 1-1.
  42. Hayashi, K. et al. (2020) The Natural History of Spontaneously Occurred Endometriosis in Cynomolgus Monkeys by Monthly Follow-Up Laparoscopy for Two Years.
    Tohoku J Exp Med. 251 (4): 241-53.
  43. Gonçalves, V.M. et al. (2021) Macrophage and Lymphocyte Infiltration Is Associated with Volumetric Tumor Size but Not with Volumetric Growth in the T&yyml;bingen Schwannoma Cohort.
    Cancers (Basel). 13 (3): 466.
  44. Pestronk, A. (2020) Chronic Graft Versus Host Myopathies: Noninflammatory, Multi-Tissue Pathology With Glycosylation Disorders.
    J Neuropathol Exp Neurol. 79 (1): 102-12.
  45. Holsapple, J.S. et al. (2021) Low Intensity Shockwave Treatment Modulates Macrophage Functions Beneficial to Healing Chronic Wounds.
    Int J Mol Sci. 22(15):7844.
  46. da Silva M.C.M. et al. (2021) Inhibition of CSF1R, a receptor involved in microglia viability, alters behavioral and molecular changes induced by cocaine.
    Sci Rep. 11 (1): 15989.
  47. Frafjord, A. et al. (2020) Antibody combinations for optimized staining of macrophages in human lung tumours.
    Scand J Immunol. 92 (1): e12889.
  48. Ma, H. et al. (2021) Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndrome virus-2 infection in vitro..
    Vet Res. 52 (1): 97.
  49. Kemmerer, C.L. et al. (2021) Cerebrospinal fluid cytokine levels are associated with macrophage infiltration into tumor tissues of glioma patients
    BMC Cancer. 21(1):1108.
  50. Lehmann, M. et al. (2021) New onset of mainly guttate psoriasis after COVID-19 vaccination: a case report.
    J Eur Acad Dermatol Venereol. 35 (11): e752-e755.
  51. Baumann, D. et al. (2021) p38 MAPK signaling in M1 macrophages results in selective elimination of M2 macrophages by MEK inhibition.
    J Immunother Cancer.9 (7): e002319.
  52. Ikezumi, Y. et al. (2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.
    Pediatr Nephrol. 36 (2): 349-59.
  53. Eligini, S. et al. (2019) Biological profile of monocyte-derived macrophages in coronary heart disease patients: implications for plaque morphology.
    Sci Rep. 9 (1): 8680.
  54. Edwards, J.H. et al. (2021) Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.
    Biomaterials. 279: 121204.
  55. Ströbel, S. et al. (2021) A 3D primary human cell-based in vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates.
    Sci Rep. 11 (1): 22765.
  56. Galea, I. et al. (2022) Iron Deposition in the Brain After Aneurysmal Subarachnoid Hemorrhage.
    Stroke. 53 (5): 1633-42.

Immunoassay

Immunofluorescence

Immunohistology - Paraffin

RRID
AB_2074540
UniProt
Q86VB7
Entrez Gene
CD163
GO Terms
GO:0005515 protein binding
GO:0006953 acute-phase response
GO:0005576 extracellular region
GO:0005044 scavenger receptor activity
GO:0005887 integral to plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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