CD16 antibody | DJ130c
Mouse anti Human CD16 antibody, clone DJ130c (MCA2537) used for the detection of CD16 on in vitro polarized human macrophages by flow cytometry.
Image caption:
Expression of phenotypic markers on in vitro polarized human macrophages. Healthy peripheral blood monocytes were cultured for 4 days in medium or in medium supplemented with IFN-γ, IL-4, IL-10, or LPS in the absence (white bars) or presence (black bars) of HAGGs. Expression of MØIFN-γ markers CD80 and CD64, MØIL-4 markers CD200R and CD14, and MØIL-10 markers CD163, CD16 and CD32 was measured by flow cytometry. Bars represent the mean ± SEM of 4 independent experiments. *p<0.05, **p<0.01, #p<0.01.
From: Ambarus CA, Santegoets KCM, van Bon L, Wenink MH, Tak PP, et al. (2012)
Soluble Immune Complexes Shift the TLR-Induced Cytokine Production of Distinct Polarized Human Macrophage Subsets towards IL-10.
PLoS ONE 7(4): e35994.
Characterization of human monocyte subsets using Mouse anti Human CD16 antibody, clone DJ130c (MCA2537) by flow cytometry.
Image caption:
Monocyte subsets and expression of phenotypic markers on spondyloarthritis (SpA) and rheumatoid arthritis (RA) patients and healthy donor monocytes. The percentage of CD14+CD16-, CD14+CD16+, and CD14dimCD16+ monocytes from SpA and RA patients and healthy donors was measured with flow cytometry (A). Expression of CD64, CD200R, and CD16 on monocytes from SpA and RA patients and healthy donors was measured with flow cytometry and expressed as geometric mean fluorescence intensity (gMFI). Data are represented as median and interquartile range of at least seven independent experiments (B).
From: Ambarus CA, Noordenbos T, de Hair MJ, Tak PP, Baeten DL. Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis. Arthritis Res Ther. 2012 Apr 11;14(2):R74.
Characterization of CD16 expression on in vitro polarized macrophages by flow cytometry using Mouse anti Human CD16 antibody, clone DJ130c.
Image caption:
Expression of phenotypic markers on in vitro polarized monocyte-derived macrophages from spondyloarthritis (SpA) and rheumatoid arthritis (RA) patients and healthy individuals. The expression of MΦIFN-γ markers CD80 and CD64, MØIL-4 markers CD200R and CD14, and MøIL-10 markers CD163 and CD16 was measured with flow cytometry on in vitro polarized monocyte-derived macrophages. Data are expressed as geometric mean fluorescence intensity (gMFI) of the marker expression after polarization with IFN-γ, IL-4, or IL-10, respectively, divided by the gMFI of the marker expression on unpolarized macrophages. Data are represented as median and interquartile range of at least five independent experiments.
From: Ambarus CA, Noordenbos T, de Hair MJ, Tak PP, Baeten DL.
Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 2012 Apr 11;14(2):R74.
Mouse anti Human CD16:Alexa Fluor® 488
Mouse anti Human CD16:Alexa Fluor® 647
Mouse anti Human CD16:Biotin
Mouse anti Human CD16
Mouse anti Human CD16:RPE
Mouse anti Human CD16:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody
- Clone
- DJ130c
- Isotype
- IgG1
Mouse anti Human CD16 antibody, clone DJ130c recognizes human CD16, also known as Low affinity immunoglobulin gamma Fc region receptor III-A or Fc-gamma RIIIa. CD16a is a 254 amino acid ~50-65 kDa single pass type 1 transmembrane glycoprotein bearing two Ig-like C2 type domains. CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form, Fc gammaRIIIB, or CD16B (Scallon et al. 1989). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes (Ravetch and Perussia 1989). In addition, CD16B exists as two allelic variants NA1 and NA2 . DJ130c recognizes all polymorphonuclear cells irrespective of their NA phenotype.
Mouse anti Human CD16 antibody, clone DJ130c recognizes an epitope in the first membrane-distal domain of CD16, recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta (Xu et al. 2012)
Mouse anti Human CD16 antibody, clone DJ130c recognizes an epitope in the first membrane-distal domain of CD16, recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta (Xu et al. 2012)
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Macaque - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor®488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Reconstitution
- Reconstitute with 1.0ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of reconstitution.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P08637 Related reagents
- O75015 Related reagents
- Entrez Gene
- FCGR3B Related reagents
- FCGR3A Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0005576 extracellular region
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0031225 anchored to membrane
- GO:0019864 IgG binding
- GO:0016021 integral to membrane
- GO:0050776 regulation of immune response
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD16 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/5 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/20 Pack Size: 25 µg 1/50 Pack Size: 0.2 mg |
1/200 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | Neat Pack Size: 25 Tests/0.25ml 1/10 Pack Size: 100 Tests/1ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Histology Positive Control Tissue
- Human tonsil
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | ||
| Mouse IgG1 Negative Control:RPE | MCA928PE | 100 Tests | F | ||
| Mouse IgG1 Negative Control:Alexa Fluor® 700 | MCA928A700 | 100 Tests/1ml | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD16 antibody
-
Schmidt, R.E. (1993) CD16 cluster workshop report.
In Leucocyte Typing V: White cell differentiation antigens, Vol.1. Edited by Schlossman, S.F. et al. Oxford University Press. p805 – 806. -
Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.
Diab Vasc Dis Res. 8: 221-8. -
Shantsila, E. et al. (2012) Fibrinolytic status in acute coronary syndromes: evidence of differences in relation to clinical features and pathophysiological pathways.
Thromb Haemost. 108: 32-40. -
Shantsila, E. et al. (2011) Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
J Thromb Haemost. 9: 1056-66. -
Tapp, L.D. et al. (2012) The CD14++CD16+ monocyte subset and monocyte-platelet interactions in patients with ST-elevation myocardial infarction.
J Thromb Haemost. 10: 1231-41. -
Ambarus, C.A. et al. (2012) Intimal lining layer macrophages but not synovial sublining macrophages display an IL-10 polarized-like phenotype in chronic synovitis.
Arthritis Res Ther. 14: R74. -
Ambarus, C.A. et al. (2012) Systematic validation of specific phenotypic markers for in vitro polarized human macrophages.
J Immunol Methods. 375: 196-206. -
Ambarus, C.A. et al. (2012) Soluble immune complexes shift the TLR-induced cytokine production of distinct polarized human macrophage subsets towards IL-10.
PLoS One. 7: e35994. -
Shantsila, E. et al. (2012) The effects of exercise and diurnal variation on monocyte subsets and monocyte-platelet aggregates.
Eur J Clin Invest. 42: 832-9. -
Chehadeh. W. et al. (2009) Antibody-mediated opsonization of red blood cells in parvovirus B19 infection.
Virology. 390: 56-63. -
Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.
Circ Heart Fail. 6: 127-35. -
Jaipersad, A.S. et al. (2014) Expression of monocyte subsets and angiogenic markers in relation to carotid plaque neovascularization in patients with pre-existing coronary artery disease and carotid stenosis.
Ann Med. 11: 1-9. -
Shantsila, E. et al. (2015) Free Light Chains in patients with acute coronary syndromes: Relationships to inflammation and renal function.
Int J Cardiol. 185: 322-7. -
Wrigley, B.J. et al. (2013) Increased formation of monocyte-platelet aggregates in ischemic heart failure.
Circ Heart Fail. 6 (1): 127-35. -
Romee R et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17).
Blood. 121 (18): 3599-608. -
Sousa, S. et al. (2015) Human breast cancer cells educate macrophages toward the M2 activation status.
Breast Cancer Res. 17: 101.
