CD14 antibody | TÜK4
Phycoerythrin conjugated Mouse anti Human CD14 antibody, clone Tük4 (MCA1568PE) used for the evaluation of CD14 expression on bovine monocytes by flow cytometry.
Image caption:
Gating strategy utilized for flow cytometric analysis of bovine peripheral blood leukocytes showing representative data from one animal. After gating on viable (propidium-iodide-negative) cells (A), cell doublets were excluded by SSC-A and SSC-H gating (B). Bovine mononuclear cells (MNC) and granulocytes (PMN) were gated based on their forward and side scatter characteristics and their percentages were calculated (C). Three-color immunofluorescence of bovine MNC with mAbs to CD172a, CD14 and CD16 defines three monocyte subsets in peripheral blood. Viable mononuclear cells, based on forward and side scatter characteristics, were gated on CD172a-positive cells (D). Dot plots of CD14 and CD16 expression display classical monocytes (CD14+CD16−, upper left), intermediate monocytes (CD14+CD16+, upper right) and nonclassical monocytes (CD14−CD16+, lower right) (E)
From: Pomeroy B, Sipka A, Hussen J, Eger M, Schukken Y, Schuberth HJ.
Counts of bovine monocyte subsets prior to calving are predictive for postpartum occurrence of mastitis and metritis.
Vet Res. 2017 Feb 21;48(1):13.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Image caption:
CD11b+CD14−MHCII− cells suppress T cell proliferation. Facs sorted CD11b+CD14−MHCII− cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.
From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody clone Tük4 (MCA1568) used to evaluate CD14 expression on canine monocytes by flow cytometry.
Image caption:
Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs. PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b+CD14−MHCII− cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b+CD14−MHCII− cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b+CD14−MHCII− cells is shown at 63× magnification.
From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody clone Tük4 (MCA1568) used to evaluate CD14 expression on canine monocytes by flow cytometry.
Image caption:
CD11b+CD14+MHCII− cells demonstrate ability to suppressive T cell proliferation. (A) CD11b+CD14+MHCII− cells were sorted from peripheral blood sample of an osteosarcoma dog (B) and co-cultured with healthy dog PBMCs in the presence of mitogen for 72 hs. Non-stimulated PBMCs were used as negative control and PBMCs co-cultured with healthy PMNs were used to control for the effect of adding cells to the assay. Proliferative responses were measured by 3H-thymidine incorporation. CPM, counts per minute. The experiment was performed in triplicate. Mean ± SEM are shown.
From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used to identify bovine monocytes in the skin and subcutaneous tissue of adjuvant injected calves by immunofluorescence.
Image caption:
Cellular recruitment to skin and subcutaneous tissues. (A) HE stained sections of skin with subcutaneous tissue from the side injected with adjuvant and the contralateral side, at 24 h post-injection. Scale bars: 200 μm. (B) Enlargement of outlined areas in A, as indicated. Scale bars: 20 μm. (C) Immunofluorescent labeling of subcutaneous tissue on the injected side with antibody against CD14 (green). Scale bar: 20 μm.
From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)
Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front. Immunol. 7:322.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used to identify bovine monocytes in lymph nodes of adjuvant injected calves by immunohistochemistry.
Image caption:
Cellular recruitment to lymph nodes (LN) and peripheral blood. (A) LN cells were prepared for FCM analysis and gated on forward/side scatter (FSC/SSC) characteristics. Plots from one representative animal are presented. Panels illustrate the gating of lymphocytes, monocytes, and granulocytes as indicated and in the draining LN (left) and the contralateral LN (right), at 24 h post-injection. (B) Percentages of major immune cell populations in LNs, based on the gating strategy in A. Horizontal stacked bars show mean percentages of lymphocytes (gray), monocytes (dark gray), and granulocytes (black) of the total live cell population in non-injected animals (n = 6) and at different time points after adjuvant injection (n = 2–3). (C) IHC labeling of draining and contralateral LNs at 24 h post-injection with antibody against CD14. Different regions of the LN are indicated. (D) Cellular differential counts in peripheral blood. Horizontal stacked bars show mean absolute numbers (×109) of lymphocytes (gray), monocytes (dark gray), and granulocytes (black) at pre-injection and at different time points after adjuvant injection.
From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)
Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front. Immunol. 7:322.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used to identify bovine monocytes in lymph nodes of adjuvant injected calves by immunofluorescence.
Image caption:
Distribution of monocytes in the LNs. Immunofluorescent labeling of LNs with antibodies against CD14 (green), CD21 (blue), and Ki67 (red). CD21 stains the LN follicles. (A) CD14+ cells were present in the capsule, subcapsular sinus, peri-trabecular sinus, and interfollicular areas of the draining LN at 24 h post-injection. (B) The contralateral LN was mainly devoid of CD14+ cells. Note the empty sub capsular sinus and trabecular sinus areas, as opposed to the infiltration in (A). (C) CD14+ cells were abundant in the capsule, but were decreased in numbers in the sinus and the cortex at 48 h post-injection. (D) CD14+ cells were present in the medulla of the LN at 48 h post-injection, and particularly around vessels. Follicle (f), interfollicular area (i), capsule (c), sinus area (s), vessel (v). Scale bars: 100 μm.
From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)
Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front. Immunol. 7:322.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used to identify bovine monocytes and Mouse anti Bovine CD205 (MCA1651GA) to identify dendritic cells in lymph nodes and subcutaneous tissue of adjuvant injected calves by immunofluorescence.
Image caption:
Distribution of monocyte and DC markers in skin and LNs. Immunofluorescent labeling of subcutaneous tissues and LNs with antibodies against CD14 (green), CD205 (red) and CD11c (blue), at 24 h post-injection. (A) Large numbers of CD14+ cells were observed in the subcutis on the injected side. Note the separation of collagen bundles (appears as gray auto fluorescence, arrowheads) due to inflammatory infiltrates. A limited number of CD205+ cells were present. (B) A few CD14+ cells (arrow) were observed on the contralateral side. (C) CD14+ cells infiltrated the interfollicular areas of the cortex of the draining LN. A moderate amount of CD205+ CD11c+ DCs were observed (arrow heads in insert). (D) CD14+ cells were sparsely present in the contralateral LN. CD205+ follicles were surrounded by CD11c single labeled and CD11c+ CD205+ double labeled cells. Follicle (f), interfollicular area (i), capsule (c), sinus area (s). Scale bars: 100 μm.
From: Lund H, Boysen P, Åkesson CP, Lewandowska-Sabat AM and Storset AK (2016)
Transient Migration of Large Numbers of CD14++ CD16+ Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front. Immunol. 7:322.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
RPE-AlexaFluor647 tandem conjugated Mouse anti Human CD14 antibody, clone Tük4 (MCA1568P647) used to identify CD14 expressing mononuclear cells in peripheral blood samples by flow cytometery.
Image caption:
Clinical relevance of CD11b+CD14+S100A9+ MDSCs in lung adenocarcinoma harboring activating EGFR mutation
(A) Representative dot plots of PBMC of NSCLC patients (CA) and normal health donors (NC). Non-lymphocyte mononuclear cell was gated, and CD11b+CD14+ S100A9+ cells were analyzed by flow cytometry. (B) Ratio of CD11b+CD14+ S100A9+ cells in PBMC of health donors (NC, n = 7) and NSCLC patients (CA, n = 58). (C) Clinical response of EGFR-TKI treatment and peripheral blood CD11b+CD14+ S100A9+ MDSCs, CR+PR, n = 46; SD+ PD, n = 12. (D) CD11b+CD14+ S100A9+ difference between DCB and NCB groups in training cohort. (DCB, n = 19; NCB, n = 6, p = 0.0007) (E) CD11b+CD14+ S100A9+ difference between DCB and NCB groups in validation cohort. (DCB, n = 26; NCB, n = 7, p = 0.06) (F–H) Kaplain-Meier curve of PFS according to median percentage of CD11b+CD14+S100A9+ in training cohort, validation cohort and all cases. Dark line: CD11b+CD14+S100A9+ ≤18.9% in PBMC, dashed line: CD11b+CD14+S100A9+ >18.9%. All data express as mean ±SD, * p <0.05; CR: complete response, PR: partial response, SD: stable disease, PD: progress disease, DCB: durable clinical benefit, NDB: non-durable benefit.
From: Feng PH, Yu CT, Chen KY, Luo CS, Wu SM, Liu CY, Kuo LW, Chan YF, Chen TT, Chang CC, Lee CN, Chuang HC, Lin CF, Han CL, Lee WH, Lee KY.
S100A9(+) MDSC and TAM-mediated EGFR-TKI resistance in lung adenocarcinoma: the role of RELB.
Oncotarget. 2018 Jan 10;9(7):7631-43.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
RPE conjugated Mouse anti Bovine CD14 antibody, clone Tük4 (MCA1568PE) used to identify monocyte populations in bovime blood samples by flow cytometry.
Image caption:
Changes in the monocyte subsets of PBMCs in Holstein and Jersey steers subjected to heat stress. Flow cytometry analysis to identify monocyte subset population. A dot plot depicting the three monocyte subsets in monocytes (CD172a+): classical CD14+CD16-, intermediate CD14+CD16+, and non-classical CD14-CD16+ monocytes. MT indicates moderate THI condition and HT indicates high THI condition
From: Park DS, Gu BH, Park YJ, Joo SS, Lee SS, Kim SH, Kim ET, Kim DH, Lee SS, Lee SJ, Kim BW, Kim M.
Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 2021 Jul;26(4):705-20.
doi: 10.1007/s12192-021-01216-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody, clone Tük4 (MCA1568) used for the characterization of rabbit bone marrow derived mesenchymal stem cells using flow cytometry
Image caption:
Phenotypic characterization of BMSCs using flow cytometry
From: Wee JH, Yoo KD, Sim SB, Kim HJ, Kim HJ, Park KN, Kim GH, Moon MH, You SJ, Ha MY, Yang DH, Chun HJ, Ko JH, Kim CH.
Stem cell laden nano and micro collagen/PLGA bimodal fibrous patches for myocardial regeneration.
Biomater Res. 2022 Dec 13;26(1):79.
doi: 10.1186/s40824-022-00319-w.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
R-Phycoerythrin conjugated Mouse anti Human CD14 antibody, clone TÜK4 (MCA1568PE) used for the characterization of rabbit mesenchymal stem cells by flow cytometry.
Image caption:
Flow cytometry analysis. Adherent cells that showed MSC morphology were characterized using specific markers for stem cells according to the International Society for Cellular Therapy (ISCT). Negative controls of each antibody’s own isotype were included to rule out nonspecific signals and unlabeled cells (dotted line curves). CD73, CD44, and CD105 stained datasets were found positive after overlay, whereas CD34 and CD14 stained datasets were negative, thus discarding a hematopoietic origin of the sampled cells and suggesting an MSC phenotype.
From: Arnaud-Franco Á, Lara-Arias J, Marino-Martínez IA, Cienfuegos-Jiménez O, Barbosa-Quintana Á, Peña-Martínez VM.
Effect of Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Application in Achilles-Tendon Injury in an Animal Model.
Curr Issues Mol Biol. 2022 Nov 22;44(12):5827-38.
doi: 10.3390/cimb44120396.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Pacific Blue® conjugated Mouse anti Human CD14 antibody, clone TÜK4 (MCA1568PB) used to label lapine monocytes for analysis by flow cytometry.
Image caption:
Analysis of rabbit peripheral blood mononuclear cells by multi-colour flow cytometry. PBMC were isolated from K3EDTA-sampled blood of naive rabbits before five-colour flow cytometry analysis. Specific detection of monocytes, B cells and T cell subsets consisted in a 3-step staining procedure. PBMC were first stained with anti-rabbit IgM (or mouse IgG1 isotype control), anti-rabbit CD8 (or mouse IgG1 isotype control) and anti-rabbit CD4 (or mouse IgG2a isotype control). Stainings were revealed with PE-conjugated rat anti-IgG1 or biotinylated rat anti-IgG2a, as secondary staining. Final staining was performed with streptavidin-APC, FITC-conjugated anti-rabbit T lymphocytes (or FITC-conjugated mouse IgG1 isotype control) and anti-human CD14 (or Pacific Blue-conjugated mouse IgG2a isotype control). Live lymphocytes were gated on 7-AAD- cells.
From: Dewals BG, Vanderplasschen A.
Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4-
T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 2011 Aug 22;42(1):95.
doi: 10.1186/1297-9716-42-95.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody, clone TüK4 (MCA1568) used to label sheep monocytes by flow cytometry.
Image caption:
Gating Strategy forFlow Cytometry:
More that 10,000 events were acquired ba a Macsquant flow cytometer for each sample and analysed with Flowlogic software using the following strategy.
The main cell population was selected using side scatter - Area (SS-A) versus FSC-A in P1 gate. Doublet cells were excluded in gate P2. Cells from P1/P2 gate were analsed for the specific staining as shown in histogram overlays showing staining with specific antibodies (in red) in comparison with their respective isotypes (in blue). Markers (M, M1, M2, M3, M4, M5) were set up according to the control isotypes to determine the percentage of positive cells. The same strategy was applied to all samples.
From: Ducournau C, Moiré N, Carpentier R, Cantin P, Herkt C, Lantier I, Betbeder D, Dimier-Poisson I.
Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
Front Immunol. 2020 Sep 9;11:2183.
doi: 10.3389/fimmu.2020.02183.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody, clone TÜK4 (MCA1568) used to label canine CD14 expressing cells by immunofluorescence.
Image caption:
Structural organization of canine Peyer’s patches, according to immunofluorescence data.(D) Myeloid cell staining. Immunostaining of the CD14 and CD11c markers, along with CD21, was used to identify dendritic cells within the SED. Different images from the lower and upper parts of the sample were captured to adequately show the CD11c staining in proximity to both the epithelium and the follicle.
From: Miguelena Chamorro B, Hameed SA, Dechelette M, Claude JB, Piney L, Chapat L, Swaminathan G, Poulet H, Longet S, De Luca K, Mundt E, Paul S.
Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 2023 Nov 1;7(11):788-805.
doi: 10.4049/immunohorizons.2300091.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD14 antibody, clone TÜK4 (MCA1568) used to label canine CD14 expressing cells by immunofluorescence.
Image caption:
Structural organization of canine Peyer’s patches, according to immunofluorescence data.(D) Myeloid cell staining. Immunostaining of the CD14 and CD11c markers, along with CD21, was used to identify dendritic cells within the SED. Different images from the lower and upper parts of the sample were captured to adequately show the CD11c staining in proximity to both the epithelium and the follicle.
From: Miguelena Chamorro B, Hameed SA, Dechelette M, Claude JB, Piney L, Chapat L, Swaminathan G, Poulet H, Longet S, De Luca K, Mundt E, Paul S.
Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 2023 Nov 1;7(11):788-805.
doi: 10.4049/immunohorizons.2300091.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
StarBright Violet 790 conjugated Mouse anti Human CD14 antibody, clone TÜK4 (MCA1568SBV790) used for the gating of milk neutrophils by flow cytometry
Image caption:
Hierarchical gating to identify milk neutrophils bearing the FcμR and expression by migrated neutrophils. Milk cells were isolated by centrifugation from milk samples taken from inflamed glands (> 300 000 cells/mL). (A) The cells were labeled with antibodies to CD14-SBV790, or to granulocytes (MM20A) and F(ab’)2 anti-mouse IgG AF546, or anti-peptide 1 and F(ab’)2 anti-rabbit-PE.
From: Gilbert FB, Rainard P.
Expression of the receptor for IgM (FcμR) by bovine neutrophils.
Dev Comp Immunol. 2024 Jul 30:105235 [Epub ahead of print].
doi: 10.1016/j.dci.2024.105235. ].
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD14
- Product Type
- Monoclonal Antibody
- Clone
- TÜK4
- Isotype
- IgG2a
- Specificity
- CD14
Antibody Quality and Performance Guarantee
| Mouse anti Human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen. CD14 is a ~55 kDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage (Simmons et al. 1989), a soluble form of CD14 also exists (Bazil et al. 1986). CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Pugin et al. 1994, Dziarski et al. 1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria. Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner (Yang et al. 2003). Use of the anti-human CD14 antibody, Low Endotoxin format is recommended for this purpose. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Goat Cat Rabbit Mink Bovine Pig Sheep Cynomolgus monkey Llama - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- MCA1568: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- MCA1568T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA1568GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/50 | 1/200 |
| Immunofluorescence | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells or 100μl whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG2a Negative Control | ||||||
References for CD14 antibody
-
Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species.
Vet Immunol Immunopathol. 39 (4): 461-6. -
Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.
Vet Immunol Immunopathol. 51 (1-2): 89-99. -
Sopp, P. & Howard, C.J. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells.
Vet Immunol Immunopathol. 56 (1-2): 11-25. -
Werling, D. et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus.
Vet Immunol Immunopathol. 62 (3): 185-95. -
Weiss, D.J. (2001) Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies.
Vet Pathol. 38: 512-8. -
Bryan, S.A. et al. (2002) Responses of leukocytes to chemokines in whole blood and their antagonism by novel CC-chemokine receptor 3 antagonists.
Am J Respir Crit Care Med. 165: 1602-9. -
Yang, H. et al. (2003) Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion.
Immunol Lett. 88 (1): 27-30. -
Schenk, M. et al. (2005) Macrophages expressing triggering receptor expressed on myeloid cells-1 are underrepresented in the human intestine.
J Immunol. 174 (1): 517-24.
View The Latest Product References
-
Fulton, B.E. Jr. et al. (2006) Dissemination of bovine leukemia virus-infected cells from a newly infected sheep lymph node.
J Virol. 80: 7873-84. -
Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
J Virol. 81: 9665-79. -
Dewals, B.G. & Vanderplasschen, A. (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 42 (1): 95. -
Dalli J et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
Blood. 112 (6): 2512-9. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin Vaccine Immunol. 16 (7): 1003-11. -
Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705. -
Xiong, W. et al. (2010) Human Flt3L generates dendritic cells from canine peripheral blood precursors: implications for a dog glioma clinical trial.
PLoS One. 5: e11074. -
Kallapur, S.G. et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95. -
Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
Vet Immunol Immunopathol. 159 (1-2): 91-6. -
Schaut, R.G. et al. (2015) Bovine viral diarrhea virus type 2 in vivo infection modulates TLR4 responsiveness in differentiated myeloid cells which is associated with decreased MyD88 expression.
Virus Res. 208: 44-55. -
Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
Vet Comp Oncol. 14 (4): 409-16. -
Gibson, A.J. et al. (2016) Differential macrophage function in Brown Swiss and Holstein Friesian cattle.
Vet Immunol Immunopathol. 181: 15-23. -
Krueger, L.A. et al. (2016) Gamma delta T cells are early responders to Mycobacterium avium ssp. paratuberculosis in colostrum-replete Holstein calves.
J Dairy Sci. 99 (11): 9040-50. -
Lund, H. et al. (2016) Transient Migration of Large Numbers of CD14(++) CD16(+) Monocytes to the Draining Lymph Node after Onset of Inflammation.
Front Immunol. 7: 322. -
Westover, A.J. et al. (2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
J Diabetes Res. 2016: 3486727. -
Pomeroy, B. et al. (2017) Counts of bovine monocyte subsets prior to calving are predictive for postpartum occurrence of mastitis and metritis.
Vet Res. 48 (1): 13. -
Martini, V. et al. (2018) Flow cytometry for feline lymphoma: a retrospective study regarding pre-analytical factors possibly affecting the quality of samples.
J Feline Med Surg. 20 (6): 494-501. -
Feng, P.H. et al. (2018) S100A9+ MDSC and TAM-mediated EGFR-TKI resistance in lung adenocarcinoma: the role of RELB.
Oncotarget. 9 (7): 7631-43. -
Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
Vet Immunol Immunopathol. 202: 74-84. -
Lessard, M. et al. (2018) Piglet weight gain during the first two weeks of lactation influences the immune system development.
Vet Immunol Immunopathol. 206: 25-34. -
Moncada-Saucedo, N.K. et al. (2019) A Bioactive Cartilage Graft of IGF1-Transduced Adipose Mesenchymal Stem Cells Embedded in an Alginate/Bovine Cartilage Matrix Tridimensional Scaffold.
Stem Cells Int. 2019: 9792369. -
Kolar, Q.K. et al. (2020) Anatomical distribution of respiratory tract leukocyte cell subsets in neonatal calves.
Vet Immunol Immunopathol. 227: 110090. -
Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
Comp Immunol Microbiol Infect Dis. 68: 101388. -
Muñoz-Silvestre, A. et al. (2020) Pathogenesis of Intradermal Staphylococcal Infections: Rabbit Experimental Approach to Natural Staphylococcus aureus Skin Infections.
Am J Pathol. 190 (6): 1188-210. -
Sipka, A.S. et al. (2020) The effect of ex vivo. lipopolysaccharide stimulation and nutrient availability on transition cow innate immune cell AKT/mTOR pathway responsiveness.
J Dairy Sci. 103 (2): 1956-1968. -
Mas, A. et al. (2020) A further investigation of the leishmaniosis outbreak in Madrid (Spain): low-infectivity phenotype of the Leishmania infantum BOS1FL1 isolate to establish infection in canine cells.
Vet Immunol Immunopathol. 230: 110148. -
Schwarz, E.R. et al. (2020) Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.
Viruses. 12 (3): 291. -
Penadés, M. et al. (2020) Early deviations in performance, metabolic and immunological indicators affect stayability in rabbit females.
Animal. 14 (4): 780-9. -
Tuohy, J.L. et al. (2020) Immune dysregulation and osteosarcoma: Staphylococcus aureus. downregulates TGF-β and heightens the inflammatory signature in human and canine macrophages suppressed by osteosarcoma.
Vet Comp Oncol. 18 (1): 64-75. -
Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 26 (4): 705-20. -
Grudzien, M. et al. (2021) A newly established canine NK-type cell line and its cytotoxic properties.
Vet Comp Oncol. 19 (3): 567-77. -
Jaensch, S.M. et al. (2022) Clinicopathologic and immunophenotypic features in dogs with presumptive large granular lymphocyte leukaemia.
Aust Vet J. 100 (11): 527-32. -
Riccardo, F. et al. (2022) Antigen mimicry as an effective strategy to induce CSPG4-targeted immunity in dogs with oral melanoma: a veterinary trial.
J Immunother Cancer. 10(5):e004007. -
Shiue, S.J. et al. (2022) Arthrospira Enhances Seroclearance in Patients with Chronic Hepatitis B Receiving Nucleos(t)ide Analogue through Modulation of TNF-α/IFN-γ Profile.
Nutrients. 14 (14): 2790. -
Wee, J.H. et al. (2022) Stem cell laden nano and micro collagen/PLGA bimodal fibrous patches for myocardial regeneration.
Biomater Res. 26 (1): 79. -
Arnaud-Franco, Á. et al. (2022) Effect of Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Application in Achilles-Tendon Injury in an Animal Model.
Curr Issues Mol Biol. 44 (12): 5827-38. -
Ashwood, P. (2022) Preliminary Evidence of Differentially Induced Immune Responses by Microparticle-adsorbed LPS in Patients with Crohn's Disease.
J Cell Immunol. 4 (6): 211-218. -
Rotolo, A. et al. (2023) Unedited allogeneic iNKT cells show extended persistence in MHC-mismatched canine recipients.
Cell Rep Med. 4 (10): 101241. -
Rütgen, B.C. et al. (2022) Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats.
J Feline Med Surg. 24 (2): 77-90. -
Ducournau, C. et al. (2020) Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep
Front Immunol. 11:2183. -
Sheng, R. et al. (2023) Prognostic significance of CD25 expression in dogs with a noninvasive diagnosis of B-cell lymphoma treated with CHOP chemotherapy.
Vet Comp Oncol. 21 (1): 28-35. -
Miguelena Chamorro, B. et al. (2023) Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 7 (11): 788-805. -
Mason, N.J. et al. (2021) Development of a fully canine anti-canine CTLA4 monoclonal antibody for comparative translational research in dogs with spontaneous tumors.
MAbs. 13 (1): 2004638. -
Gilbert, F.B. & Rainard, P. (2024) Expression of the receptor for IgM (FcμR) by bovine neutrophils.
Dev Comp Immunol. : 105235. 30 Jul [Epub ahead of print].
Further Reading
-
Bazil, V. et al. (1986) Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.
Eur J Immunol. 16:1583-9. -
Simmons, D. L. et al. (1989) Monocyte antigen CD14 is a phospholipid anchored membrane protein.
Blood. 73:284-9. -
Pugin, J. et al. (1994) CD14 is a pattern recognition receptor.
Immunity.1:509-16. -
Dziarski, R. et al. (1998) Binding of bacterial peptidoglycan to CD14.
J Biol Chem. 273:8680-90. -
Jersmann, H.P. (2005) Time to abandon dogma: CD14 is expressed by non-myeloid lineage cells.
Immunol Cell Biol. 83:462-7. -
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- RRID
- AB_566515
- UniProt
- P08571
- Entrez Gene
- CD14
- GO Terms
- GO:0005886 plasma membrane
- GO:0001530 lipopolysaccharide binding
- GO:0001847 opsonin receptor activity
- GO:0006915 apoptosis
- GO:0006909 phagocytosis
- GO:0006954 inflammatory response
- GO:0008063 Toll signaling pathway
- GO:0031225 anchored to membrane
- GO:0016019 peptidoglycan receptor activity
- View More GO Terms
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0045087 innate immune response
- GO:0070891 lipoteichoic acid binding
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071223 cellular response to lipoteichoic acid
View more products with CD14 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up