CD33 antibody | WM53
Mouse anti Human CD33:StarBright Violet 790
- Product Type
- Monoclonal Antibody
- Clone
- WM53
- Isotype
- IgG1
- Specificity
- CD33
Antibody Quality and Performance Guarantee
Figure B. FITC conjugated Mouse anti Human CD14 antibody, clone UCHM1 (MCA596F) and Alexa Fluor®647 conjugated Mouse anti Human CD33 antibody, clone WM33 (MCA1271A647).
All experiments performed on red cell lysed human peripheral blood gated on myeloid cells in the presence of Human SeroBlock (BUF070A).
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568A647) and StarBright Violet 515 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV515). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Mouse anti Human CD33 antibody, clone WM53 (MCA1271) used to evaluate CD33 expression on blood derived NK cells by flow cytometry.
Image caption:
CD33 expression and function in primary human NK cells. (a) Blood NK cells (CD3-CD56+ lymphocytes in region R1) and (b, c) quiescent primary human NK cells were phenotyped by flow cytometry. Blood CD33+ NK cells were mainly CD56bright (R2 region, dark dots), CD16-, CD57-, NKG2A+, and NKG2D+. The figure shows a representative blood sample out of the four tested.
From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.
CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019 Apr 15;2019:6032141.
doi: 10.1155/2019/6032141.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD33 antibody, clone WM53 (MCA1271) used to evaluate CD33 expression on blood derived NK cells using immunoprecipitation.
Image caption:
CD33 expression and function in primary human NK cells. (d) CD33 coprecipitated SHP-2 phosphatase only in quiescent primary human NK cell lysates. The figure shows a representative experiment out of two with similar results.
From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.
CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019 Apr 15;2019:6032141.
doi: 10.1155/2019/6032141.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Human CD33 antibody, clone WM53 (MCA1271) used to evaluate CD33 expression on blood derived quiescent NK cells by flow cytometry.
Image caption:
CD33 expression and function in primary human NK cells.CD33+ (WM53+ HIM3-4-) quiescent NK cells (R1 region, dark dots) were CD57-, NKG2D+, and partially expressed NKp46 and NKG2A antigens.
From: Hernández-Caselles T, Miguel RC, Ruiz-Alcaraz AJ, García-Peñarrubia P.
CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019 Apr 15;2019:6032141.
doi: 10.1155/2019/6032141.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568A488) and StarBright Violet 670 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV670). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568A488) and StarBright Violet 440 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV440). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568A488) and StarBright Violet 610 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV610). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 647 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568A647) and StarBright Violet 570 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV570). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. RPE-Alexa Fluor® 750 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568P750) and StarBright Violet 710 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV710). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. FITC conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568F) and StarBright Violet 790 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV790). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568A488) and StarBright Violet 475 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV475). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. FITC conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568F) and StarBright UltraViolet 400 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV400). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 510 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBUV510) and StarBright Violet 760 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV760). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 475 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV475) and StarBright UltraViolet 665 conjugated Mouse anti Human CD33 antibody, clone wM53 (MCA1271SBUV665). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 710 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV710) and StarBright UltraViolet 445 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV445). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 790 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV790) and StarBright UltraViolet 445 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBUV445). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568A700) and StarBright UltraViolet 795 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV795). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 488 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568A488) and StarBright UltraViolet 510 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV510). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 790 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV790) and StarBright UltraViolet 510 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBUV510). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV440) and StarBright UltraViolet 575 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV575). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV440) and StarBright UltraViolet 605 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV605). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B StarBright Violet 440 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV440) and StarBright Blue 580 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBB580). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B Alexa Fluor® 700 conjugated Mouse anti Human CD14 (MCA1568A700) and StarBright Blue 580 conjugated Mouse anti Human CD33 (MCA1271SBB580). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Fluor® 700 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568A700) and StarBright Blue 580 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBB580). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV440) and StarBright Blue 675 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBB675). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV400) and StarBright Blue 675 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBB675). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBUV400) and StarBright Blue 765 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBB765). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 665 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV665) and StarBright Blue 810 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBB810). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV440) and StarBright Blue 810 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBB810). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBUV795) and StarBright Yellow 575 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBY575). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 670 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV670) and StarBright Yellow 575 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBY575). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV440) and StarBright Yellow 720 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBY720). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV610) and StarBright Yellow 720 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBY720). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV440) and StarBright Yellow 605 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBY605). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright UltraViolet 795 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV795) and StarBright Yellow 605 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBY605). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 570 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV570) and StarBright Yellow 800 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBY800). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 515 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV515) and StarBright Yellow 800 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBY800). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 670 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV670) and StarBright Yellow 665 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBY665). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (MCA1568SBV440) and StarBright Yellow 665 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBY665). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 515 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV515) and StarBright Red 775 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBR775). All experiments performed on red cell lysed human blood gated on live single cell mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 515 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV515) and StarBright Red 775 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD33 antibody, clone VM53 (MCA1271SBV440) and StarBright Red 815 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBR815). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV440) and StarBright Red 815 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBR815). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Yellow 605 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBY605) and StarBright UltraViolet 740 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBUV740). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 580 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBB580) and StarBright UltraViolet 740 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBUV740). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBV440) and StarBright Red 715 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBR715). All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum..
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 610 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBV440) and StarBright Red 715 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBR715). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Blue 615 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBB615) and StarBright Red 670 conjugated Mouse anti Human CD33 antibody, clone WM53 (MCA1271SBR670). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Figure B. StarBright Violet 440 conjugated Mouse anti Human CD33 antibody, clone VM53 (MCA1271SBB615) and StarBright Red 670 conjugated Mouse anti Human CD14 antibody, clone TüK4 (MCA1568SBR670).
All experiments performed on red cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum.
Data acquired on the ZE5 Cell Analyzer.
Filter by Application:
F IP WB Reset| Mouse anti Human CD33 antibody, clone WM53, is a monoclonal antibody that recognizes CD33, also known as Siglec-3 or gp67. Clone WM53 detects CD33 in humans and has been validated in the following applications: flow cytometry, immunoprecipitation and western blotting. Clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets. CD33 is a single pass type I membrane protein and member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells, but is absent on lymphocytes, normal platelets, hematopoietic stem cells and erythrocytes. Upon CD33 activation, the immunoreceptor tyrosine-based inhibition motif (ITIM) is phosphorylated by Src family kinases, resulting in recruitment of SH2 domain containing phosphatases and inhibition of cellular activation. As sialoglycans are present on almost all human cells, CD33 and other members of the siglec family have a role in discriminating self from non-self. Mouse anti human CD33 antibody, clone WM53, is also available as a recombinant antibody (TZA1271). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to StarBright Violet 790 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- Human AML cells
- Approx. Protein Concentrations
- For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 790 402 782 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
Source Reference
-
Favaloro, E.J. et al. (1987) Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, gp67 (CD-33).
Dis Markers. 5 (4): 215-25.
References for CD33 antibody
-
Favaloro, E.J. et al. (1988) Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33.
Br J Haematol. 69 (2): 163-71. -
Favaloro, E.J. et al. (1993) Differential expression of surface antigens on activated endothelium.
Immunol Cell Biol. 71:571-81. -
Pietschmann, P. et al. (2000) Surface markers and transendothelial migration of dendritic cells from elderly subjects.
Exp Gerontol. 35: 213-24. -
Vamvakopoulos, J. et al. (2002) Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.
Eur J Immunol. 32 (10): 2988-96. -
Vamvakopoulos, J.E. & Green, C. (2003) HMG-CoA reductase inhibition aborts functional differentiation and triggers apoptosis in cultured primary human monocytes: a potential mechanism of statin-mediated vasculoprotection.
BMC Cardiovasc Disord. 3: 6. -
Dahl C et al. (2004) Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells cultured in vitro.
Allergy. 59 (10): 1087-96. -
Lajaunias, F. et al. (2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling.
Eur J Immunol. 35: 243-51. -
Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
Blood. 106 (10): 3567-74.
View The Latest Product References
-
Hernández-Caselles, T. et al. (2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
J Leukoc Biol. 79: 46-58. -
Biedermann, B. et al. (2006) Analysis of the CD33-related siglec family reveals that Siglec-9 is an endocytic receptor expressed on subsets of acute myeloid leukemia cells and absent from normal hematopoietic progenitors.
Leuk Res. 31: 211-20. -
Yasukawa, T. et al. (2012) Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis.
Anal Chem. 84 (20): 8830-6. -
McCormack E et al. (2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.
Blood. 121 (7): e34-42. -
Hu, Z. et al. (2016) Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibody
J Wuhan Univ of Technol-Mater. Sci. Ed. 31 (2): 446-53. -
Hernández-Caselles T et al. (2019) CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
J Immunol Res. 2019: 6032141.
- Synonyms
- SIGLEC-3
- UniProt
- P20138
- Entrez Gene
- CD33
- GO Terms
- GO:0007155 cell adhesion
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0007267 cell-cell signaling
- GO:0008285 negative regulation of cell proliferation
MCA1271SBV790
100005608
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