CD11b antibody | ICRF44

Mouse anti Human CD11b

Product Type
Monoclonal Antibody
Clone
ICRF44
Isotype
IgG1
Specificity
CD11b

Product Code Applications Pack Size List Price Your Price Qty
MCA551
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IP 0.2 mg loader
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loader
MCA551T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F 20 µg loader
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loader
MCA551GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IP 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD11b antibody, clone ICRF44 recognizes the human CD11b cell surface glycoprotein, a 165 kDa molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.

Mouse anti Human CD11b antibody, clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Cynomolgus monkey
Baboon
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA551, MCA551GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA551T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rheumatoid synovial cells and human monocytes
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/100
Immunohistology - Frozen 1 1/100 1/1000
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Histology Positive Control Tissue
Human tonsil

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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)

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Description Mouse IgG1 Negative Control

References for CD11b antibody

  1. Yoshino, M. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49: 97-110.
  2. Jonker, M. et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocyte.
    Leucocyte Typing IV. Oxford University Press 1058-1063.
  3. Dransfield, I. et al. (1992) Interaction of leukocyte integrins with ligand is necessary but not sufficient for function.
    J Cell Biol. 116 (6): 1527-35.
  4. Malhotra, V. et al. (1986) Ligand binding by the p150,95 antigen of U937 monocytic cells: properties in common with complement receptor type 3 (CR3).
    Eur J Immunol. 16 (9): 1117-23.
  5. Glasow, A. et al. (2005) Retinoids and myelomonocytic growth factors co-operatively activate RAR{alpha} and induce human myeloid leukemia cell differentiation via MAP kinase pathways.
    Blood 105: 341-9.
  6. Rezzonico, R. et al. (2001) Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB.
    Blood. 97 (10): 2932-40.
  7. Rezzonico, R. et al. (2000) Engagement of CD11b and CD11c beta2 integrin by antibodies or soluble CD23 induces IL-1beta production on primary human monocytes through mitogen-activated protein kinase-dependent pathways.
    Blood. 95 (12): 3868-77.
  8. Canalli, A.A. et al. (2001) Participation of Mac-1, LFA-1 and VLA-4 integrins in the in vitro adhesion of sickle cell disease neutrophils to endothelial layers, and reversal of adhesion by simvastatin.
    Haematologica 96: 526-33.
  9. View The Latest Product References
  10. Patel, S. et al. (2009) Reconstituted high-density lipoprotein increases plasma high-density lipoprotein anti-inflammatory properties and cholesterol efflux capacity in patients with type 2 diabetes.
    J Am Coll Cardiol. 53: 962-71.
  11. Stirling, R.G. et al. (2001) Interleukin-5 induces CD34(+) eosinophil progenitor mobilization and eosinophil CCR3 expression in asthma.
    Am J Respir Crit Care Med. 164: 1403-9.
  12. Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
    J Pharmacol Exp Ther. 321: 656-62.
  13. Woollard, K.J. et al. (2002) Direct modulatory effect of C-reactive protein on primary human monocyte adhesion to human endothelial cells.
    Clin Exp Immunol. 130: 256-62.
  14. Ramacciotti, E. et al. (2011) Evaluation of soluble p-selectin as a marker for the diagnosis of deep venous thrombosis.
    Clin Appl Thromb Hemost. 17: 425-31.
  15. Paul, G. et al. (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells.
    PLoS One 7: e35577.
  16. Gomes-Alves, P. et al. (2016) In vitro expansion of human cardiac progenitor cells: Exploring ‘omics tools for characterization of cell-based allogeneic products
    Translational Research. 9 Feb [Epub ahead of print]
  17. Hughes, S.F. et al. (2020) The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
    Eur J Med Res. 25 (1): 68.

Flow Cytometry

Functional Assays

Synonyms
Integrin Alpha M Chain
MAC-1
RRID
AB_321288
UniProt
P11215
Entrez Gene
ITGAM
GO Terms
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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