CD105 antibody | SN6

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MCA1557
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C * F IP WB 0.2 mg loader
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MCA1557T
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Mouse anti Human CD105 antibody, clone SN6 recognizes human endoglin, also known as CD105. CD105 is a glycoprotein homodimer of ~95 kDa subunits expressed by endothelial cells, activated monocytes and some leukemia cells.
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Our CD105 (SN6) Antibody has been referenced in >73 publications*


*Based on June 2020 data from CiteAb's antibody search engine.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Cynomolgus monkey
Rhesus Monkey
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Partially purified cell membrane antigens from fresh leukemia cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Source Reference

  1. Haruta, Y. & Seon, B.K. (1986) Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.
    Proc Natl Acad Sci USA 83 (20): 7898-902.

References for CD105 antibody

  1. Pierelli, L. et al. (2000) Modulation of bcl-2 and p27 in human primitive proliferating hematopoietic progenitors by autocrine TGF-beta1 is a cell cycle-independent effect and influences their hematopoietic potential.
    Blood 95: 3001-9.
  2. May, J.E. et al. (2018) Chemotherapy-induced genotoxic damage to bone marrow cells: long-term implications.
    Mutagenesis. 33 (3): 241-251.
  3. Hauser, P.V. et al. (2010) Stem cells derived from human amniotic fluid contribute to acute kidney injury recovery.
    Am J Pathol. 177: 2011-21.
  4. Jin, H.J. et al. (2010) GD2 expression is closely associated with neuronal differentiation of human umbilical cord blood-derived mesenchymal stem cells.
    Cell Mol Life Sci. 67 (11): 1845-58.
  5. Nagano, M. et al. (2007) Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood.
    Blood 110 (1): 151-60.
  6. Braun, J. et al. (2010) Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells.
    Am J Vet Res. 71 (10): 1228-36.
  7. Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
    J Cell Physiol. 214: 75-83.
  8. Agha-Hosseini, F. et al. (2010) In vitro isolation of stem cells derived from human dental pulp.
    Clin Transplant. 24: E23-8.
  9. View The Latest Product References
  10. Arufe, M.C. et al. (2010) Chondrogenic potential of subpopulations of cells expressing mesenchymal stem cell markers derived from human synovial membranes.
    J Cell Biochem. 111: 834-45.
  11. Balmayor, E.R. et al. (2011) Synthesis and functionalization of superparamagnetic poly-ε-caprolactone microparticles for the selective isolation of subpopulations of human adipose-derived stem cells.
    J R Soc Interface 8: 896-908.
  12. Benetti, A. et al. (2008) Transforming growth factor-beta1 and CD105 promote the migration of hepatocellular carcinoma-derived endothelium.
    Cancer Res. 68: 8626-34.
  13. Ciccocioppo, R. et al. (2011) Autologous bone marrow-derived mesenchymal stromal cells in the treatment of fistulising Crohn's disease.
    Gut 60: 788-98.
  14. Cox, G. et al. (2011) The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells.
    J Bone Joint Surg Br. 93: 517-24.
  15. Ferro, F. et al. (2010) Biochemical and biophysical analyses of tissue-engineered bone obtained from three-dimensional culture of a subset of bone marrow mesenchymal stem cells.
    Tissue Eng Part A 16: 3657-67.
  16. Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.
    J Immunol. 181: 6109-16.
  17. Sallustio, F. et al. (2010) TLR2 plays a role in the activation of human resident renal stem/progenitor cells.
    FASEB J. 24: 514-25.
  18. Tso, C. et al. (2012) Phenotypic and functional changes in blood monocytes following adherence to endothelium.
    PLoS One 7: e37091.
  19. Hu, N. et al. (2013) Long-term outcome of the repair of 50 mm long median nerve defects in rhesus monkeys with marrow mesenchymal stem cells-containing, chitosan-based tissue engineered nerve grafts.
    Biomaterials 34: 100-11.
  20. Cho, H.J. et al. (2013) Generation of human secondary cardiospheres as a potent cell processing strategy for cell-based cardiac repair.
    Biomaterials 34: 651-61.
  21. Kang, S.D. et al. (2013) Isolation of Functional Human Endothelial Cells from Small Volumes of Umbilical Cord Blood.
    Ann Biomed Eng. 41: 2181-92.
  22. Mehrkens, A. et al. (2013) Non-adherent mesenchymal progenitors from adipose tissue stromal vascular fraction.
    Tissue Eng Part A 20: 1081-8.
  23. De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
    Cytometry A 81: 312-23.
  24. Zhang, J. et al. (2016) Bone mesenchymal stem cells differentiate into myofibroblasts in the tumor microenvironment.
    Oncol Lett. 12 (1): 644-50.
  25. Morsing, M. et al. (2016) Evidence of two distinct functionally specialized fibroblast lineages in breast stroma.
    Breast Cancer Res. 18 (1): 108.
  26. Williamson, K.A. et al. (2015) Restricted differentiation potential of progenitor cell populations obtained from the equine superficial digital flexor tendon (SDFT).
    J Orthop Res. 33 (6): 849-58.
  27. Lützkendorf, J. et al. (2017) Resistance for Genotoxic Damage in Mesenchymal Stromal Cells Is Increased by Hypoxia but Not Generally Dependent on p53-Regulated Cell Cycle Arrest.
    PLoS One. 12 (1): e0169921.
  28. Lee, H.J. et al. (2017) ICOSL expression in human bone marrow-derived mesenchymal stem cells promotes induction of regulatory T cells.
    Sci Rep. 7: 44486.
  29. Yi, T. et al. (2015) Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.
    Tissue Eng Part C Methods. 21 (12): 1251-62.
  30. Boccardo, S. et al. (2016) Engineered mesenchymal cell-based patches as controlled VEGF delivery systems to induce extrinsic angiogenesis.
    Acta Biomater. 42: 127-35.
  31. Mumaw, J.L. et al. (2015) Feline mesenchymal stem cells and supernatant inhibit reactive oxygen species production in cultured feline neutrophils.
    Res Vet Sci. 103: 60-9.
  32. Bertolo, A. et al. (2017) Oxidative status predicts quality in human mesenchymal stem cells.
    Stem Cell Res Ther. 8 (1): 3.
  33. GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.
  34. Olimpio, R.M.C. et al. (2018) Cell viability assessed in a reproducible model of human osteoblasts derived from human adipose-derived stem cells.
    PLoS One. 13 (4): e0194847.
  35. Lotfi, R. et al. (2018) ATP promotes immunosuppressive capacities of mesenchymal stromal cells by enhancing the expression of indoleamine dioxygenase.
    Immun Inflamm Dis. Oct 10 [Epub ahead of print].
  36. Santos,V.H.D. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
    Res Vet Sci. 124: 38-45.
  37. Noda, S. et al. (2019) Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.
    Sci Rep. 9 (1): 5430.
  38. Manini, I. et al. (2020) Heterogeneity Matters: Different Regions of Glioblastoma Are Characterized by Distinctive Tumor-Supporting Pathways.
    Cancers (Basel). 12 (10) Oct 13 [Epub ahead of print].
  39. Cargnoni, A. et al. (2020) Amniotic MSCs reduce pulmonary fibrosis by hampering lung B-cell recruitment, retention, and maturation.
    Stem Cells Transl Med. 9 (9): 1023-35.
  40. Huang, Q. et al. (2021) Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomal MicroRNA-18b-3p Inhibits the Occurrence of Preeclampsia by Targeting LEP.
    Nanoscale Res Lett. 16 (1): 27.
  41. Piñeiro-Ramil, M. et al. (2020) Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features.
    Stem Cells Int. 2020: 5726947.
  42. Kim, M. et al. (2020) A Small-Sized Population of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Shows High Stemness Properties and Therapeutic Benefit.
    Stem Cells Int. 2020: 5924983.
  43. Niu, C.C. et al. (2014) Identification of mesenchymal stem cells and osteogenic factors in bone marrow aspirate and peripheral blood for spinal fusion by flow cytometry and proteomic analysis.
    J Orthop Surg Res. 9: 32.
  44. Supokawej, A. et al. (2013) Cardiogenic and myogenic gene expression in mesenchymal stem cells after 5-azacytidine treatment.
    Turk J Haematol. 30 (2): 115-21.
  45. Kim, S.H. et al. (2019) Forkhead box O1 (FOXO1) controls the migratory response of Toll-like receptor (TLR3)-stimulated human mesenchymal stromal cells.
    J Biol Chem. 294 (21): 8424-37.
  46. Rey, F. et al. (2019) Adipose-Derived Stem Cells from Fat Tissue of Breast Cancer Microenvironment Present Altered Adipogenic Differentiation Capabilities.
    Stem Cells Int. 2019: 1480314.
  47. Lotfi, R. et al. (2020) Validation of Microbiological Testing of Cellular Medicinal Products Containing Antibiotics.
    Transfus Med Hemother. 47 (2): 144-51.
  48. Serrano, L.J. et al. (2021) Cell therapy for factor V deficiency: An approach based on human decidua mesenchymal stem cells.
    Biomed Pharmacother. 142: 112059.
  49. Piñeiro-Ramil, M. et al. (2021) Generation of Mesenchymal Cell Lines Derived from Aged Donors.
    Int J Mol Sci. 22 (19)Oct 01 [Epub ahead of print].
  50. Fernandez-Pernas, P. et al. (2017) CD105+-mesenchymal stem cells migrate into osteoarthritis joint: An animal model.
    PLoS One. 12 (11): e0188072.
  51. Di Paola, A. et al. (2021) Eltrombopag in paediatric immune thrombocytopenia: Iron metabolism modulation in mesenchymal stromal cells.
    Br J Haematol. Dec 28 [Epub ahead of print].
  52. Murata, D. et al. (2022) Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses.
    BMC Vet Res. 18 (1): 53.
  53. Orikasa, S. et al. (2022) Hypoxia-inducible factor 1α induces osteo/odontoblast differentiation of human dental pulp stem cells via Wnt/β-catenin transcriptional cofactor BCL9.
    Sci Rep. 12 (1): 682.
  54. Freitag, N. et al. (2022) Eutopic endometrial immune profile of infertility-patients with and without endometriosis
    J Repro Immunol. 29 Jan: 103489 [Epub ahead of print].
  55. Watson, L. et al. (2020) Administration of Human Non-Diabetic Mesenchymal Stromal Cells to a Murine Model of Diabetic Fracture Repair: A Pilot Study.
    Cells. 9 (6): 1394.
  56. Creamer, D.G. et al. (2022) Influence of exposure to microbial ligands, immunosuppressive drugs and chronic kidney disease on endogenous immunomodulatory gene expression in feline adipose-derived mesenchymal stem cells.
    J Feline Med Surg. : 1098612X221083074.

Further Reading

  1. Burk, J. et al. (2013) Equine cellular therapy--from stall to bench to bedside?
    Cytometry A 83 (1): 103-13.
  2. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells.
    Cell Med. 4: 1-11.

Flow Cytometry

Synonyms
Endoglin
RRID
AB_321986
UniProt
P17813
Entrez Gene
ENG
GO Terms
GO:0001300 chronological cell aging
GO:0001569 patterning of blood vessels
GO:0001937 negative regulation of endothelial cell proliferation
GO:0001947 heart looping
GO:0003084 positive regulation of systemic arterial blood pressure
GO:0007155 cell adhesion
GO:0004888 transmembrane receptor activity
GO:0005024 transforming growth factor beta receptor activity
GO:0005072 transforming growth factor beta receptor, cytoplasmic mediator activity
GO:0005114 type II transforming growth factor beta receptor binding
GO:0005534 galactose binding
GO:0005539 glycosaminoglycan binding
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0050431 transforming growth factor beta binding
GO:0009897 external side of plasma membrane
GO:0009986 cell surface
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway
GO:0022009 central nervous system vasculogenesis
GO:0022617 extracellular matrix disassembly
GO:0030155 regulation of cell adhesion
GO:0030509 BMP signaling pathway
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
GO:0030513 positive regulation of BMP signaling pathway
GO:0031953 negative regulation of protein autophosphorylation
GO:0034713 type I transforming growth factor beta receptor binding
GO:0042060 wound healing
GO:0042127 regulation of cell proliferation
GO:0042803 protein homodimerization activity
GO:0045449 regulation of transcription
GO:0048185 activin binding
GO:0048745 smooth muscle tissue development
GO:0048844 artery morphogenesis
GO:0048845 venous blood vessel morphogenesis
GO:0051001 negative regulation of nitric-oxide synthase activity
GO:0060326 cell chemotaxis
GO:0060394 negative regulation of pathway-restricted SMAD protein phosphorylation
GO:0070022 transforming growth factor beta receptor complex
GO:0070483 detection of hypoxia
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