CD105 antibody | SN6

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Mouse anti Human CD105:Alexa Fluor® 488

Mouse anti Human CD105:Alexa Fluor® 647

Mouse anti Human CD105:FITC

Mouse anti Human CD105

Mouse anti Human CD105:RPE

Mouse anti Human CD105:RPE-Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
SN6
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1557A488T F 25 Tests/0.25ml
MCA1557A488 F 100 Tests/1ml
MCA1557A647T F 25 Tests/0.25ml
MCA1557A647 F 100 Tests/1ml
MCA1557FT F 25 µg
MCA1557F F 0.1 mg
MCA1557T C* F IP WB 25 µg
MCA1557 C* F IP WB 0.2 mg
MCA1557PET F 25 Tests
MCA1557PE F 100 Tests
MCA1557P647 F 100 Tests
Mouse anti Human CD105 antibody, clone SN6 recognizes the human endoglin, also known as CD105. CD105 is a glycoprotein homodimer of ~95 kDa subunits expressed by endothelial cells, activated monocytes and some leukemia cells.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Cynomolgus monkey
Rhesus Monkey
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to RPE-Alexa Fluor 647 - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1ml distilled water
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Partially purified cell membrane antigens from fresh leukemia cells
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 Tests
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
P17813 Related reagents
Entrez Gene
ENG Related reagents
GO Terms
GO:0001300 chronological cell aging
GO:0001569 patterning of blood vessels
GO:0001937 negative regulation of endothelial cell proliferation
GO:0001947 heart looping
GO:0003084 positive regulation of systemic arterial blood pressure
GO:0007155 cell adhesion
GO:0004888 transmembrane receptor activity
GO:0005024 transforming growth factor beta receptor activity
GO:0005072 transforming growth factor beta receptor, cytoplasmic mediator activity
GO:0005114 type II transforming growth factor beta receptor binding
GO:0005534 galactose binding
GO:0005539 glycosaminoglycan binding
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0050431 transforming growth factor beta binding
GO:0009897 external side of plasma membrane
GO:0009986 cell surface
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway
GO:0022009 central nervous system vasculogenesis
GO:0022617 extracellular matrix disassembly
GO:0030155 regulation of cell adhesion
GO:0030509 BMP signaling pathway
GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
GO:0030513 positive regulation of BMP signaling pathway
GO:0031953 negative regulation of protein autophosphorylation
GO:0034713 type I transforming growth factor beta receptor binding
GO:0042060 wound healing
GO:0042127 regulation of cell proliferation
GO:0042803 protein homodimerization activity
GO:0045449 regulation of transcription
GO:0048185 activin binding
GO:0048745 smooth muscle tissue development
GO:0048844 artery morphogenesis
GO:0048845 venous blood vessel morphogenesis
GO:0051001 negative regulation of nitric-oxide synthase activity
GO:0060326 cell chemotaxis
GO:0060394 negative regulation of pathway-restricted SMAD protein phosphorylation
GO:0070022 transforming growth factor beta receptor complex
GO:0070483 detection of hypoxia
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD105 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat 1/5 Pack Size: 25 Tests/0.25ml
1/10 Pack Size: 100 Tests/1ml
Flow Cytometry Neat
Flow Cytometry 1/10 1/50
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Flow Cytometry Neat
Flow Cytometry Neat
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 100 Tests/1ml F
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 100 Tests/1ml F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F
Mouse IgG1 Negative Control:RPE-Alexa Fluor® 647 MCA928P647 100 Tests/1ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD105 antibody

  1. Haruta, Y. & Seon, B.K. (1986) Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.
    Proc Natl Acad Sci USA 83 (20): 7898-902.
  2. Jin, H.J. et al. (2010) GD2 expression is closely associated with neuronal differentiation of human umbilical cord blood-derived mesenchymal stem cells.
    Cell Mol Life Sci. 67 (11): 1845-58.
  3. Nagano, M. et al. (2007) Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood.
    Blood 110 (1): 151-60.
  4. Braun, J. et al. (2010) Evaluation of the osteogenic and chondrogenic differentiation capacities of equine adipose tissue-derived mesenchymal stem cells.
    Am J Vet Res. 71 (10): 1228-36.
  5. Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells.
    J Cell Physiol. 214: 75-83.
  6. Agha-Hosseini, F. et al. (2010) In vitro isolation of stem cells derived from human dental pulp.
    Clin Transplant. 24: E23-8.
  7. Arufe, M.C. et al. (2010) Chondrogenic potential of subpopulations of cells expressing mesenchymal stem cell markers derived from human synovial membranes.
    J Cell Biochem. 111: 834-45.
  8. Balmayor, E.R. et al. (2011) Synthesis and functionalization of superparamagnetic poly-ε-caprolactone microparticles for the selective isolation of subpopulations of human adipose-derived stem cells.
    J R Soc Interface 8: 896-908.
  9. Benetti, A. et al. (2008) Transforming growth factor-beta1 and CD105 promote the migration of hepatocellular carcinoma-derived endothelium.
    Cancer Res. 68: 8626-34.
  10. Ciccocioppo, R. et al. (2011) Autologous bone marrow-derived mesenchymal stromal cells in the treatment of fistulising Crohn's disease.
    Gut 60: 788-98.
  11. Cox, G. et al. (2011) The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells.
    J Bone Joint Surg Br. 93: 517-24.
  12. Ferro, F. et al. (2010) Biochemical and biophysical analyses of tissue-engineered bone obtained from three-dimensional culture of a subset of bone marrow mesenchymal stem cells.
    Tissue Eng Part A 16: 3657-67.
  13. Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.
    J Immunol. 181: 6109-16.
  14. Sallustio, F. et al. (2010) TLR2 plays a role in the activation of human resident renal stem/progenitor cells.
    FASEB J. 24: 514-25.
  15. Tso, C. et al. (2012) Phenotypic and functional changes in blood monocytes following adherence to endothelium.
    PLoS One 7: e37091.
  16. Hu, N. et al. (2013) Long-term outcome of the repair of 50 mm long median nerve defects in rhesus monkeys with marrow mesenchymal stem cells-containing, chitosan-based tissue engineered nerve grafts.
    Biomaterials 34: 100-11.
  17. Cho, H.J. et al. (2013) Generation of human secondary cardiospheres as a potent cell processing strategy for cell-based cardiac repair.
    Biomaterials 34: 651-61.
  18. Kang, S.D. et al. (2013) Isolation of Functional Human Endothelial Cells from Small Volumes of Umbilical Cord Blood.
    Ann Biomed Eng. 41: 2181-92.
  19. Mehrkens, A. et al. (2013) Non-adherent mesenchymal progenitors from adipose tissue stromal vascular fraction.
    Tissue Eng Part A 20: 1081-8.
  20. De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
    Cytometry A 81: 312-23.
  21. Zhang, J. et al. (2016) Bone mesenchymal stem cells differentiate into myofibroblasts in the tumor microenvironment.
    Oncology Letters. May 30 [Epub ahead of print]
  22. Morsing, M. et al. (2016) Evidence of two distinct functionally specialized fibroblast lineages in breast stroma.
    Breast Cancer Res. 18 (1): 108.
  23. Williamson, K.A. et al. (2015) Restricted differentiation potential of progenitor cell populations obtained from the equine superficial digital flexor tendon (SDFT).
    J Orthop Res. 33 (6): 849-58.
  24. Lützkendorf, J. et al. (2017) Resistance for Genotoxic Damage in Mesenchymal Stromal Cells Is Increased by Hypoxia but Not Generally Dependent on p53-Regulated Cell Cycle Arrest.
    PLoS One. 12 (1): e0169921.
  25. Lee, H.J. et al. (2017) ICOSL expression in human bone marrow-derived mesenchymal stem cells promotes induction of regulatory T cells.
    Sci Rep. 7: 44486.
  26. Yi, T. et al. (2015) Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.
    Tissue Eng Part C Methods. 21 (12): 1251-62.
  27. Boccardo, S. et al. (2016) Engineered mesenchymal cell-based patches as controlled VEGF delivery systems to induce extrinsic angiogenesis.
    Acta Biomater. 42: 127-35.
  28. Mumaw, J.L. et al. (2015) Feline mesenchymal stem cells and supernatant inhibit reactive oxygen species production in cultured feline neutrophils.
    Res Vet Sci. 103: 60-9.
  29. Bertolo, A. et al. (2017) Oxidative status predicts quality in human mesenchymal stem cells.
    Stem Cell Res Ther. 8 (1): 3.
  30. GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.
  31. Olimpio, R.M.C. et al. (2018) Cell viability assessed in a reproducible model of human osteoblasts derived from human adipose-derived stem cells.
    PLoS One. 13 (4): e0194847.

Further Reading

  1. Burk, J. et al. (2013) Equine cellular therapy--from stall to bench to bedside?
    Cytometry A 83 (1): 103-13.
  2. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells.
    Cell Med. 4: 1-11.
CiteAb logo - trusted, tested, published

Our CD105 (SN6) Antibody has been referenced in >60 publications*


*Based on February 2018 data from CiteAb's antibody search engine.