C4d antibody | 057-51.5.1.6
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Mouse anti Human C4d (Neoantigen) antibody, clone 057-51.5.1.6 recognizes a neoantigen on the secreted protein complement component 4d (C4d). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. The short half life of C4d means that it is present in serum at high enough concentrations to make it a useful marker for classical complement activation.
C4 has been linked to susceptibility to systemic lupus erythematosous.
C4 has been linked to susceptibility to systemic lupus erythematosous.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Purified human C4d.
- Approx. Protein Concentrations
- IgG concentration 1.07 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
Applications of C4d antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptoccal glomerulonephritis patients
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Secondary Antibodies Available
Product Specific References
References for C4d antibody
-
Rogers, J. et al. (1992) Complement activation by beta-amyloid in Alzheimer disease.
Proc Natl Acad Sci U S A. 89 (21): 10016-20.
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