Pan B-Cells antibody | CVS36




Mouse anti Horse Pan B-Cells:FITC
Mouse anti Horse Pan B-Cells
Mouse anti Horse Pan B-Cells:RPE
- Product Type
- Monoclonal Antibody
- Clone
- CVS36
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1899F | F | 0.1 mg |
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MCA1899GA | E F | 0.1 mg |
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MCA1899PE | F | 100 Tests |
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Specific anti equine reagents have yet to be fully characterized for the typically recognized B-cell makers such as CD19, CD20, CD21, CD22 and CD79. While testing has demonstrated the cross reactivity of some monoclonal B-cell markers raised against other species with equine B cells, such as the Mouse anti Human CD79a antibody, (clone HM57), an overview of which may be found in the report of the second equine leucocyte antigen workshop (Lunn et al. 1998).
With specificity for equine Ig light chains, clone CVS36 may be used to detect all equine immunoglobulin classes and subclasses in ELISA applications (Lunn et al. 1998).
Product Details
- Target Species
- Horse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Purified Equine Ig.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 18 months from date of despatch
- Guarantee
- 18 months from date of despatch
- Guarantee
- 12 months from date of reconstitution
More Information
- Regulatory
- For research purposes only
Applications of Pan B-Cells antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
ELISA | |||
Flow Cytometry | 1/25 | 1/200 | |
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Secondary Antibodies Available
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Horse Pan B-Cells | MCA1899GA | E F | 0.1 mg |
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Mouse anti Horse Pan B-Cells:RPE | MCA1899PE | F | 100 Tests |
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Mouse anti Horse Pan B-Cells:FITC | MCA1899F | F | 0.1 mg |
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Mouse anti Horse Pan B-Cells:RPE | MCA1899PE | F | 100 Tests |
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Product Specific References
References for Pan B-Cells antibody
-
Schneider, R. (2010) Analysis of antibody subtypes and T lymphocyte activation following vaccination of healthy foals against West Nile Virus
In M.Sc thesis University of Pennsylvania, Chapter 3, p.34-45 -
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43. -
Umlauf, C. (2004) Herstellung und Charakterisierung monoklonaler Antikörper gegen equine Leukozyten
In Phd thesis Ludwig-Maximillians-Universität München -
Tomlinson, J.E. et al. (2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.
Vet Immunol Immunopathol. 199: 22-31.
Further Reading
-
Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets.
Immunology. 74 (2): 251-7. -
Sheoran, A.S. et al. (1998) Monoclonal antibodies to subclass-specific antigenic determinants on equine immunoglobulin gamma chains and their characterization.
Vet Immunol Immunopathol. 62 (2): 153-65.