MHC Class II Monomorphic antibody | CVS20




Mouse anti Horse MHC Class II Monomorphic:FITC
Mouse anti Horse MHC Class II Monomorphic
Mouse anti Horse MHC Class II Monomorphic:RPE
- Product Type
- Monoclonal Antibody
- Clone
- CVS20
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1085F | F | 0.1 mg |
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MCA1085GA | C F IP | 0.1 mg |
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MCA1085PE | F | 100 Tests |
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The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this is referred to as the equine leukocyte antigen (ELA) region.
Product Details
- Target Species
- Horse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Bovine Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- 3132 cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X.63-Ag8.653 mouse myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- Regulatory
- For research purposes only
Applications of MHC Class II Monomorphic antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Horse MHC Class I Monomorphic:FITC | MCA1086F | F | 0.1 mg |
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Mouse anti Horse MHC Class I Monomorphic | MCA1086GA | C F IP | 0.1 mg |
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Product Specific References
References for MHC Class II Monomorphic antibody
-
Kydd, J.H. & Antczak. D.F. (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991
Equine Immunol. 4: 5. -
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43. -
Weiss, D.J. et al. (2001) Regulation of expression of major histocompatibility antigens by bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium.
Infect Immun. 69 (2): 1002-8. -
Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
Immunology. 105 (4): 499-508. -
Carrade, D.D. et al. (2011) Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses.
Cytotherapy. 13: 419-30. -
Catchpole, B. et al. (2002) Generation of blood-derived dendritic cells in dogs with oral malignant melanoma.
J Comp Pathol. 126: 238-41. -
Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
Vet Pathol. 43: 127-35. -
Weiss, D.J. (2001) Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies.
Vet Pathol. 38: 512-8. -
Sassa, Y. et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc
Vet Immunol Immunopathol. 133: 33-9. -
Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
Cell Med. 4 (1): 1-11. -
Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
Comp Immunol Microbiol Infect Dis. 45: 40-7. -
Maia, L. et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
Cell Biol Int. Nov 26 [Epub ahead of print] -
de Moraes, C.N. et al. (2016) Bovine endometrial cells: a source of mesenchymal stem/progenitor cells.
Cell Biol Int. 40 (12): 1332-1339. -
Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
Front Immunol. 7: 392. -
Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
Dev Comp Immunol. 65: 352-7. -
Abdelhamid, L. et al. (2017) Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells.
Res Vet Sci. 114: 225-32. -
Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
Theriogenology. 100: 8-15. -
Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
Comp Immunol Microbiol Infect Dis. 45: 40-7. -
Santos, V.H.D. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
Res Vet Sci. 124: 38-45. -
Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 9 (1): 96.
Further Reading
-
Burk, J. et al. (2013) Equine cellular therapy-from stall to bench to bedside?
Cytometry A. 83: 103-13