MHC Class II Monomorphic antibody | CVS20
Mouse anti Equine MHC Class II antibody, clone CVS20 (MCA1085GA) used to identify equine mesenchymal stem cells by flow cytometry.
Image caption:
Representative image of equine ASC phenotype. Equine ASC phenotype panel. Positive markers: CD44, CD29, CD90, and MHC I. Negative markers: F6B and MHC II.
Barberini DJ, Aleman M, Aristizabal F, Spriet M, Clark KC, Walker NJ, Galuppo LD, Amorim RM, Woolard KD, Borjesson DL.
Safety and tracking of intrathecalallogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 2018 Apr 10;9(1):96.
doi: 10.1186/s13287-018-0849-6.
From:
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
R-phycoerythrin conjugated Mouse anti Horse MHC Class II Monomorphic antibody, clone CVS20 (MCA1085PE) used to identify equine lymphocytes in total equine blood cell preparation by flow cytometry.
Image caption:
Gating strategy to determine fractions of leucocytes (a–e).
(a) Viable, propidium iodide-negative cells were determined in FL3 versus SSC-A density plots.; (b) Single cells among viable cells were identified in FSC-A versus FSC-H density plots; (c) A region of granulocytes, lymphocytes, and monocytes were identified in FSC-A versus SSC-A density plots. Fractions of lymphoid cell subpopulations were determined after dual staining with antibodies specific for CD4 and CD8 or MHC II and CD21 to identify CD4+ and CD8+ lymphocytes (d) or CD21+ B-cells and CD21−MHC-II+ lymphocytes (e).
From: Lucassen A, Finkler-Schade C, Schuberth H-J. A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
Animals. 2021; 11(9):2726.
doi: 10.3390/ani11092726
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Insert meta-description and applications
Image caption:
Higher MHC class II abundance in PBMC from MAP-resistant compared with persistently MAP-infected cows as measured by flow cytometry analysis with representative histograms. (B2) Higher mean fluorescence intensity of MHC class II positive PBMC from MAP-resistant compared to persistently MAP-infected cows. Mean fluorescence intensity from flow cytometry ± SD.
From:Korbonits L, Kleinwort KJH, Amann B, Didier A, Märtlbauer E, Hauck SM, Deeg CA.
Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome.
Metabolites. 2022 Sep 29;12(10):924.
doi: 10.3390/metabo12100924..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
RPE conjugated Mouse anti Horse MHC class II antibody, clone CVS20 (MCA1085PE) used to identify equine lymphocyte populations by flow cytometry.
Image caption:
Gating strategy for determination of leukocyte fractions (a–e) using density plots. (a) Viable, propidium iodide-negative leukocytes were identified according to their morphology and in a PI/SSC density plot. (b) Single cells were detected among viable cells in an FSC-A/FSC-H density plot. (c) Regions identifying viable, single granulocytes, monocytes, and lymphocytes in a FSC-A/SSC density plot. Identification of CD4+ and CD8+ T cells among lymphoid cells after a staining with directly labelled CD4- and CD8-specific antibodies (d) or CD21+ B cells and MHC-II+CD21− lymphocytes after staining with directly labelled MHC-II− and CD21-specific antibodies (e).
From: Terpeluk, E.R.; Schäfer, J.; Finkler-Schade, C.; Schuberth, H.-J.
Supplementation of Foals with a Saccharomyces cerevisiae Fermentation Product Alters the Early Response to Vaccination.
Animals 2024, 14, 960.
doi: 10.3390/ani14060960
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
RPE conjugated Mouse anti Horse MHC Class II Monomorphic antibody, clone CVS20 (MCA1085PE) demonstrating negative staining on equine mesenchymal stem cells as analysed by flow cytometry.
Image caption:
MSC were harvested, stained with fluorescent-labeled monoclonal antibodies
The figure displays representative histograms of surface marker stainings (red) and the corresponding isotype controls (blue).
From: Moellerberndt J, Niebert S, Fey K, Hagen A, Burk J.
Impact of platelet lysate on immunoregulatory characteristics of equine mesenchymal stromal cells.
Front Vet Sci. 2024 Apr 24;11:1385395.
doi: 10.3389/fvets.2024.1385395.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Horse MHC Class II Monomorphic
- Product Type
- Monoclonal Antibody
- Clone
- CVS20
- Isotype
- IgG1
- Specificity
- MHC Class II Monomorphic
Antibody Quality and Performance Guarantee
| Mouse anti Horse MHC Class II Monomorphic antibody, clone CVS20 recognizes monomorphic equine MHC Class II and was classified at the International Equine Leucocyte Antigen Workshop. Clone CVS20 reacts with all equine B cells and 95% of equine T cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this is referred to as the equine leukocyte antigen (ELA) region. |
- Target Species
- Horse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Bovine Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- 3132 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X.63-Ag8.653 mouse myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for MHC Class II Monomorphic antibody
-
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43. -
Weiss, D.J. et al. (2001) Regulation of expression of major histocompatibility antigens by bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium.
Infect Immun. 69 (2): 1002-8. -
Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
Immunology. 105 (4): 499-508. -
Catchpole, B. et al. (2002) Generation of blood-derived dendritic cells in dogs with oral malignant melanoma.
J Comp Pathol. 126: 238-41. -
Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
Vet Pathol. 43: 127-35. -
Sassa, Y. et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc
Vet Immunol Immunopathol. 133: 33-9. -
Carrade, D.D. et al. (2011) Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses.
Cytotherapy. 13: 419-30. -
Weiss, D.J. (2001) Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies.
Vet Pathol. 38: 512-8.
View The Latest Product References
-
Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
Cell Med. 4 (1): 1-11. -
Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
Comp Immunol Microbiol Infect Dis. 45: 40-7. -
Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
Comp Immunol Microbiol Infect Dis. 45: 40-7. -
de Moraes, C.N. et al. (2016) Bovine endometrial cells: a source of mesenchymal stem/progenitor cells.
Cell Biol Int. 40 (12): 1332-1339. -
Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
Front Immunol. 7: 392. -
Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
Dev Comp Immunol. 65: 352-7. -
Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
Theriogenology. 100: 8-15. -
Maia, L. et al. (2017) Conditioned medium: a new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
Cell Biol Int. 41 (3): 239-48. -
Abdelhamid, L. et al. (2017) Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells.
Res Vet Sci. 114: 225-32. -
Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 9 (1): 96. -
Dos Santos, V.H. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
Res Vet Sci. 124: 38-45. -
Witonsky, S. et al. (2019) Can levamisole upregulate the equine cell-mediated macrophage (M1) dendritic cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro.?
J Vet Intern Med. 33 (2): 889-96. -
Lopez, B.S. et al. (2019) The effect of age on foal monocyte-derived dendritic cell (MoDC) maturation and function after exposure to killed bacteria.
Vet Immunol Immunopathol. 210: 38-45. -
Lucassen, A. et al. (2021) A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
Animals (Basel). 18;11(9):2726. -
Korbonits, L. et al. (2022) Mycobacterium avium subsp. paratuberculosis Infected Cows Reveal Divergent Immune Response in Bovine Peripheral Blood Derived Lymphocyte Proteome.
Metabolites. 12 (10): 924.. -
Lopez, B.S. et al. (2020) Phenotypic characterization of equine monocyte-derived dendritic cells generated ex vivo utilizing commercially available serum-free medium.
Vet Immunol Immunopathol. 222: 110036. -
Lopez, B.S. et al. (2020) The effect of foal or adult horse plasma on equine monocyte-derived dendritic cell phenotype and function.
Vet Immunol Immunopathol. 228: 110099. -
Lopez,.B.S. et al. (2024) The effect of cortisol on equine monocyte–derived dendritic cell phenotype and cytokine production
Vet Med Sci. 10 (2): e1333 [Epub ahead of print]. -
Cabezas, J. et al. (2020) In vitro preconditioning of equine adipose mesenchymal stem cells with prostaglandin E(2), substance P and their combination changes the cellular protein secretomics and improves their immunomodulatory competence without compromising stemness.
Vet Immunol Immunopathol. 228: 110100. -
Terpeluk, R.E. et al. (2024) Supplementation of Foals with a Saccharomyces cerevisiae Fermentation Product Alters the Early Response to Vaccination
Animals. 14 (6): 960. -
Kamm, J.L. et al. (2021) Immune response to allogeneic equine mesenchymal stromal cells.
Stem Cell Res Ther. 12 (1): 570. -
Espinoza-Duarte, M.R. et al. (2022) Flow cytometry analysis of CD11c-positive peripheral blood mononuclear cells in horses.
Vet Immunol Immunopathol. 253: 110504. -
Moellerberndt, J. et al. (2024) Impact of platelet lysate on immunoregulatory characteristics of equine mesenchymal stromal cells.
Front Vet Sci. 11: 1385395.
Further Reading
-
Burk, J. et al. (2013) Equine cellular therapy-from stall to bench to bedside?
Cytometry A. 83: 103-13
MCA1085GA
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