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MHC Class II Monomorphic antibody | CVS20

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Anti Horse MHC Class II Monomorphic Antibody, clone CVS20 thumbnail image 1 Anti Horse MHC Class II Monomorphic Antibody, clone CVS20 thumbnail image 2 Anti Horse MHC Class II Monomorphic Antibody, clone CVS20 thumbnail image 3 Anti Horse MHC Class II Monomorphic Antibody, clone CVS20 thumbnail image 4
Anti Horse MHC Class II Monomorphic Antibody, clone CVS20 gallery image 1
Staining of equine peripheral blood lymphocytes with Mouse anti Equine MHC Class II (MCA1085GA) followed by Goat anti Mouse IgG:FITC (STAR117F)
Anti Horse MHC Class II Monomorphic Antibody, clone CVS20 gallery image 2
Staining of equine peripheral blood lymphocytes with Mouse anti Equine MHC Class II:FITC (MCA1085F)
Anti Horse MHC Class II Monomorphic Antibody, clone CVS20 gallery image 3
Staining of equine peripheral blood lymphocytes with Mouse anti Equine MHC Class II:RPE (MCA1085PE)
Anti Horse MHC Class II Monomorphic Antibody, clone CVS20 gallery image 4
Published customer image:
Mouse anti Equine MHC Class II antibody, clone CVS20 (MCA1085GA) used to identify equine mesenchymal stem cells by flow cytometry.
Image caption:
Representative image of equine ASC phenotype. Equine ASC phenotype panel. Positive markers: CD44, CD29, CD90, and MHC I. Negative markers: F6B and MHC II.
Barberini DJ, Aleman M, Aristizabal F, Spriet M, Clark KC, Walker NJ, Galuppo LD, Amorim RM, Woolard KD, Borjesson DL.
Safety and tracking of intrathecalallogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 2018 Apr 10;9(1):96.
doi: 10.1186/s13287-018-0849-6.
From:
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Horse MHC Class II Monomorphic:FITC

Mouse anti Horse MHC Class II Monomorphic

Mouse anti Horse MHC Class II Monomorphic:RPE

Product Type
Monoclonal Antibody
Clone
CVS20
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1085F
Datasheet
F
SDS
0.1 mg loader

MCA1085GA
Datasheet
C F IP
SDS
0.1 mg loader

MCA1085PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Horse MHC Class II Monomorphic antibody, clone CVS20 recognizes monomorphic equine MHC Class II and was classified at the International Equine Leucocyte Antigen Workshop. Clone CVS20 reacts with all equine B cells and 95% of equine T cells.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this is referred to as the equine leukocyte antigen (ELA) region.

Product Details

Target Species
Horse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Bovine
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Buffer Solution
Phosphate buffered saline.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
3132 cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X.63-Ag8.653 mouse myeloma cell line

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class II Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Horse MHC Class I Monomorphic:FITC MCA1086F F 0.1 mg loader
Mouse anti Horse MHC Class I Monomorphic MCA1086GA C F IP 0.1 mg loader

Application Based External Images

Flow Cytometry

Product Specific References

References for MHC Class II Monomorphic antibody

  1. Kydd, J.H. & Antczak. D.F. (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991
    Equine Immunol. 4: 5.
  2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62 (2): 101-43.
  3. Weiss, D.J. et al. (2001) Regulation of expression of major histocompatibility antigens by bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium.
    Infect Immun. 69 (2): 1002-8.
  4. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
    Immunology. 105 (4): 499-508.
  5. Carrade, D.D. et al. (2011) Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses.
    Cytotherapy. 13: 419-30.
  6. Catchpole, B. et al. (2002) Generation of blood-derived dendritic cells in dogs with oral malignant melanoma.
    J Comp Pathol. 126: 238-41.
  7. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
    Vet Pathol. 43: 127-35.
  8. Weiss, D.J. (2001) Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies.
    Vet Pathol. 38: 512-8.
  9. Sassa, Y. et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc
    Vet Immunol Immunopathol. 133: 33-9.
  10. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
    Cell Med. 4 (1): 1-11.
  11. Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
    Comp Immunol Microbiol Infect Dis. 45: 40-7.
  12. Maia, L. et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
    Cell Biol Int. Nov 26 [Epub ahead of print]
  13. de Moraes, C.N. et al. (2016) Bovine endometrial cells: a source of mesenchymal stem/progenitor cells.
    Cell Biol Int. 40 (12): 1332-1339.
  14. Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
    Front Immunol. 7: 392.
  15. Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
    Dev Comp Immunol. 65: 352-7.
  16. Abdelhamid, L. et al. (2017) Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells.
    Res Vet Sci. 114: 225-32.
  17. Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
    Theriogenology. 100: 8-15.
  18. Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
    Comp Immunol Microbiol Infect Dis. 45: 40-7.
  19. Dos Santos, V.H. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
    Res Vet Sci. 124: 38-45.
  20. Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
    Stem Cell Res Ther. 9 (1): 96.
  21. Witonsky, S. et al. (2019) Can levamisole upregulate the equine cell-mediated macrophage (M1) dendritic cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro.?
    J Vet Intern Med. 33 (2): 889-96.
  22. Lopez, B.S. et al. (2019) The effect of age on foal monocyte-derived dendritic cell (MoDC) maturation and function after exposure to killed bacteria.
    Vet Immunol Immunopathol. 210: 38-45.

Further Reading

  1. Burk, J. et al. (2013) Equine cellular therapy-from stall to bench to bedside?
    Cytometry A. 83: 103-13

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