MHC Class II Monomorphic antibody | CVS20

Mouse anti Horse MHC Class II Monomorphic

Product Type
Monoclonal Antibody
Clone
CVS20
Isotype
IgG1
Specificity
MHC Class II Monomorphic

Product Code Applications Pack Size List Price Your Price Qty
MCA1085GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Horse MHC Class II Monomorphic antibody, clone CVS20 recognizes monomorphic equine MHC Class II and was classified at the International Equine Leucocyte Antigen Workshop. Clone CVS20 reacts with all equine B cells and 95% of equine T cells.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this is referred to as the equine leukocyte antigen (ELA) region.

Target Species
Horse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Bovine
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
3132 cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X.63-Ag8.653 mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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References for MHC Class II Monomorphic antibody

  1. Kydd, J.H. & Antczak. D.F. (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991
    Equine Immunol. 4: 5.
  2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62 (2): 101-43.
  3. Weiss, D.J. et al. (2001) Regulation of expression of major histocompatibility antigens by bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium.
    Infect Immun. 69 (2): 1002-8.
  4. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
    Immunology. 105 (4): 499-508.
  5. Carrade, D.D. et al. (2011) Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses.
    Cytotherapy. 13: 419-30.
  6. Catchpole, B. et al. (2002) Generation of blood-derived dendritic cells in dogs with oral malignant melanoma.
    J Comp Pathol. 126: 238-41.
  7. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
    Vet Pathol. 43: 127-35.
  8. Weiss, D.J. (2001) Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies.
    Vet Pathol. 38: 512-8.
  9. View The Latest Product References
  10. Sassa, Y. et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc
    Vet Immunol Immunopathol. 133: 33-9.
  11. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
    Cell Med. 4 (1): 1-11.
  12. Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
    Comp Immunol Microbiol Infect Dis. 45: 40-7.
  13. Maia, L. et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
    Cell Biol Int. Nov 26 [Epub ahead of print]
  14. de Moraes, C.N. et al. (2016) Bovine endometrial cells: a source of mesenchymal stem/progenitor cells.
    Cell Biol Int. 40 (12): 1332-1339.
  15. Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
    Front Immunol. 7: 392.
  16. Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
    Dev Comp Immunol. 65: 352-7.
  17. Abdelhamid, L. et al. (2017) Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells.
    Res Vet Sci. 114: 225-32.
  18. Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
    Theriogenology. 100: 8-15.
  19. Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
    Comp Immunol Microbiol Infect Dis. 45: 40-7.
  20. Dos Santos, V.H. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
    Res Vet Sci. 124: 38-45.
  21. Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
    Stem Cell Res Ther. 9 (1): 96.
  22. Witonsky, S. et al. (2019) Can levamisole upregulate the equine cell-mediated macrophage (M1) dendritic cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro.?
    J Vet Intern Med. 33 (2): 889-96.
  23. Lopez, B.S. et al. (2019) The effect of age on foal monocyte-derived dendritic cell (MoDC) maturation and function after exposure to killed bacteria.
    Vet Immunol Immunopathol. 210: 38-45.
  24. Lucassen, A. et al. (2021) A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
    Animals (Basel). 18;11(9):2726.

Further Reading

  1. Burk, J. et al. (2013) Equine cellular therapy-from stall to bench to bedside?
    Cytometry A. 83: 103-13

Flow Cytometry

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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