MHC Class II Monomorphic antibody | CVS20
Mouse anti Equine MHC Class II antibody, clone CVS20 (MCA1085GA) used to identify equine mesenchymal stem cells by flow cytometry.
Image caption:
Representative image of equine ASC phenotype. Equine ASC phenotype panel. Positive markers: CD44, CD29, CD90, and MHC I. Negative markers: F6B and MHC II.
Barberini DJ, Aleman M, Aristizabal F, Spriet M, Clark KC, Walker NJ, Galuppo LD, Amorim RM, Woolard KD, Borjesson DL.
Safety and tracking of intrathecalallogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 2018 Apr 10;9(1):96.
doi: 10.1186/s13287-018-0849-6.
From:
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
R-phycoerythrin conjugated Mouse anti Horse MHC Class II Monomorphic antibody, clone CVS20 (MCA1085PE) used to identify equine lymphocytes in total equine blood cell preparation by flow cytometry.
Image caption:
Gating strategy to determine fractions of leucocytes (a–e).
(a) Viable, propidium iodide-negative cells were determined in FL3 versus SSC-A density plots.; (b) Single cells among viable cells were identified in FSC-A versus FSC-H density plots; (c) A region of granulocytes, lymphocytes, and monocytes were identified in FSC-A versus SSC-A density plots. Fractions of lymphoid cell subpopulations were determined after dual staining with antibodies specific for CD4 and CD8 or MHC II and CD21 to identify CD4+ and CD8+ lymphocytes (d) or CD21+ B-cells and CD21−MHC-II+ lymphocytes (e).
From: Lucassen A, Finkler-Schade C, Schuberth H-J. A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
Animals. 2021; 11(9):2726.
doi: 10.3390/ani11092726
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Horse MHC Class II Monomorphic
- Product Type
- Monoclonal Antibody
- Clone
- CVS20
- Isotype
- IgG1
- Specificity
- MHC Class II Monomorphic
Quick Links:
Antibody Quality and 
Unrivaled 
| Mouse anti Horse MHC Class II Monomorphic antibody, clone CVS20 recognizes monomorphic equine MHC Class II and was classified at the International Equine Leucocyte Antigen Workshop. Clone CVS20 reacts with all equine B cells and 95% of equine T cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this is referred to as the equine leukocyte antigen (ELA) region. |
- Target Species
- Horse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Bovine Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- 3132 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X.63-Ag8.653 mouse myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
References for MHC Class II Monomorphic antibody
-
Kydd, J.H. & Antczak. D.F. (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991
Equine Immunol. 4: 5. -
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43. -
Weiss, D.J. et al. (2001) Regulation of expression of major histocompatibility antigens by bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium.
Infect Immun. 69 (2): 1002-8. -
Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
Immunology. 105 (4): 499-508. -
Carrade, D.D. et al. (2011) Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses.
Cytotherapy. 13: 419-30. -
Catchpole, B. et al. (2002) Generation of blood-derived dendritic cells in dogs with oral malignant melanoma.
J Comp Pathol. 126: 238-41. -
Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease.
Vet Pathol. 43: 127-35. -
Weiss, D.J. (2001) Evaluation of proliferative disorders in canine bone marrow by use of flow cytometric scatter plots and monoclonal antibodies.
Vet Pathol. 38: 512-8.
View The Latest Product References
-
Sassa, Y. et al. (2010) Bovine macrophage degradation of scrapie and BSE PrPSc
Vet Immunol Immunopathol. 133: 33-9. -
Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
Cell Med. 4 (1): 1-11. -
Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
Comp Immunol Microbiol Infect Dis. 45: 40-7. -
Maia, L. et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
Cell Biol Int. Nov 26 [Epub ahead of print] -
de Moraes, C.N. et al. (2016) Bovine endometrial cells: a source of mesenchymal stem/progenitor cells.
Cell Biol Int. 40 (12): 1332-1339. -
Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
Front Immunol. 7: 392. -
Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
Dev Comp Immunol. 65: 352-7. -
Abdelhamid, L. et al. (2017) Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells.
Res Vet Sci. 114: 225-32. -
Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
Theriogenology. 100: 8-15. -
Hussein, H. et al. (2016) Cathepsin K inhibition renders equine bone marrow nucleated cells hypo-responsive to LPS and unmethylated CpG stimulation in vitro.
Comp Immunol Microbiol Infect Dis. 45: 40-7. -
Dos Santos, V.H. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
Res Vet Sci. 124: 38-45. -
Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 9 (1): 96. -
Witonsky, S. et al. (2019) Can levamisole upregulate the equine cell-mediated macrophage (M1) dendritic cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro.?
J Vet Intern Med. 33 (2): 889-96. -
Lopez, B.S. et al. (2019) The effect of age on foal monocyte-derived dendritic cell (MoDC) maturation and function after exposure to killed bacteria.
Vet Immunol Immunopathol. 210: 38-45. -
Lucassen, A. et al. (2021) A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
Animals (Basel). 18;11(9):2726.
Further Reading
-
Burk, J. et al. (2013) Equine cellular therapy-from stall to bench to bedside?
Cytometry A. 83: 103-13
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