MHC Class I Monomorphic antibody | CVS22






Mouse anti Horse MHC Class I Monomorphic:FITC
Mouse anti Horse MHC Class I Monomorphic
Mouse anti Horse MHC Class I Monomorphic:RPE
- Product Type
- Monoclonal Antibody
- Clone
- CVS22
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1086F | F | 0.1 mg |
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MCA1086GA | C F IP | 0.1 mg |
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MCA1086PE | F | 100 Tests |
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The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this complex is referred to as the equine leukocyte antigen (ELA) region.
Product Details
- Target Species
- Horse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Equine leucocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the X63.Ag 8.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class I Monomorphic antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen | |||
Immunoprecipitation | |||
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Horse MHC Class II Monomorphic:FITC | MCA1085F | F | 0.1 mg |
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Mouse anti Horse MHC Class II Monomorphic | MCA1085GA | C F IP | 0.1 mg |
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Product Specific References
References for MHC Class I Monomorphic antibody
-
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62:101-143 -
Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
Vet Immunol Immunopathol. 131:1-8 -
Soboll Hussey, G. et al. (2014) Innate immune responses of airway epithelial cells to infection with equine herpesvirus-1.
Vet Microbiol. 170 (1-2): 28-38. -
Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
Vet Immunol Immunopathol. 131 (1-2): 1-8. -
Maumus M et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
Front Immunol. 7: 392. -
Rapacz-leonard, A. et al. (2018) Major histocompatibility complex class I in the horse (Equus caballus) placenta during pregnancy and parturition.
Placenta. 74: 36-46. -
Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 9 (1): 96.
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