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MHC Class I Monomorphic antibody | CVS22

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Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 thumbnail image 1 Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 thumbnail image 2 Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 thumbnail image 3 Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 thumbnail image 4 Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 thumbnail image 5 Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 thumbnail image 6
Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 gallery image 1
Staining of equine peripheral blood lymphocytes with Mouse anti Equine MHC Class 1 antibody, clone CVS22 (MCA1086GA), followed by FITC conjugated Goat anti Mouse IgG antibody (STAR117F)
Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 gallery image 2
Staining of equine peripheral blood lymphocytes with FITC conjugated Mouse anti Equine MHC Class 1 antibody, clone CVS22 (MCA1086F)
Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 gallery image 3
Staining of equine peripheral blood lymphocytes with phycoerythrin conjugated Mouse anti Equine MHC Class 1 antibody, clone CVS22 (MCA1086PE)
Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 gallery image 4
Published customer image:
Mouse anti Horse MHC class I antibody, clone CVS22 (MCA1086GA) used for evaluation of MHC class I expressing white blood cells in equine samples by flow cytometry.
Image caption:
Antigen expression on WBC in CB, and CB-MSC at passage (p) 2, 3 and 5. Consistent gates were applied for all cell analyses. Control sample fluorescence is indicated by black lines, fluorescence of samples incubated with specific antibody by light blue/gray lines. Results were similar for samples from 5 animals. Numbers in each plot represent the mean proportion of cells expressing each antigen.

From: Tessier L, Bienzle D, Williams LB, Koch TG (2015)
Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells.
PLoS ONE 10(4): e0122954.
Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 gallery image 5
Figure A. RPE conjugated Mouse anti Horse CD11a/CD18 (MCA1081PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Horse CD11a/CD18 (MCA1081PE) and FITC conjugated Mouse anti Horse MHC Class I Monomorphic (MCA1086F). All experiments performed on red cell lysed horse blood gated on lymphocytes in the presence of 10% horse serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Horse MHC Class I Monomorphic Antibody, clone CVS22 gallery image 6
Published customer image:
Mouse anti Equine MHC Class I antibody, clone CVS22 (MCA1086GA) used to identify equine mesenchymal stem cells by flow cytometry.
Image caption:
Representative image of equine ASC phenotype. Equine ASC phenotype panel. Positive markers: CD44, CD29, CD90, and MHC I. Negative markers: F6B and MHC II.
Barberini DJ, Aleman M, Aristizabal F, Spriet M, Clark KC, Walker NJ, Galuppo LD, Amorim RM, Woolard KD, Borjesson DL.
Safety and tracking of intrathecalallogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 2018 Apr 10;9(1):96.
doi: 10.1186/s13287-018-0849-6.
From:
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Horse MHC Class I Monomorphic:FITC

Mouse anti Horse MHC Class I Monomorphic

Mouse anti Horse MHC Class I Monomorphic:RPE

Product Type
Monoclonal Antibody
Clone
CVS22
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1086F
Datasheet
F
SDS
0.1 mg loader

MCA1086GA
Datasheet
C F IP
SDS
0.1 mg loader

MCA1086PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Horse MHC Class I Monomorphic antibody, clone CVS22 recognizes monomorphic equine MHC Class I and was classified at the International Equine Leucocyte Antigen Workshop. MHC class I is expressed by all nucleated cells.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In horses, this complex is referred to as the equine leukocyte antigen (ELA) region.

Product Details

Target Species
Horse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Buffer Solution
Phosphate buffered saline.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Equine leucocytes.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the X63.Ag 8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class I Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Horse MHC Class II Monomorphic:FITC MCA1085F F 0.1 mg loader
Mouse anti Horse MHC Class II Monomorphic MCA1085GA C F IP 0.1 mg loader

Product Specific References

References for MHC Class I Monomorphic antibody

  1. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62:101-143
  2. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
    Vet Immunol Immunopathol. 131:1-8
  3. Soboll Hussey, G. et al. (2014) Innate immune responses of airway epithelial cells to infection with equine herpesvirus-1.
    Vet Microbiol. 170 (1-2): 28-38.
  4. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
    Vet Immunol Immunopathol. 131 (1-2): 1-8.
  5. Maumus M et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
    Front Immunol. 7: 392.
  6. Rapacz-leonard, A. et al. (2018) Major histocompatibility complex class I in the horse (Equus caballus) placenta during pregnancy and parturition.
    Placenta. 74: 36-46.
  7. Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
    Stem Cell Res Ther. 9 (1): 96.

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