CD8 antibody | CVS21
RPE conjugated Mouse anti Horse CD8 antibody, clone CVS21 (MCA1080PE) used to identify CD8 expressing cells in equine cell co-cultures by flow cytometry.
Image caption:
The figure shows systematic gating strategies based on FMO, isotype and Live/Dead controls for samples stained with CD3, CD8 and IFN-γ. A co-cultured sample of MSC and ConA-activated leukocytes was used to create the figure.
From: Hillmann A, Paebst F, Brehm W, Piehler D, Schubert S, Tárnok A, et al. (2019)
A novel direct co-culture assay analyzed by multicolor flow cytometry reveals context- and cell type-specific immunomodulatory effects of equine mesenchymal stromal cells.
PLoS ONE 14(6): e0218949.
doi: 10.1371/journal.pone.0218949
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
R-phycoerythrin conjugated Mouse anti Horse CD8 antibody, clone CVS21 (MCA1080PE) used to identify equine T cells in total equine blood cell preparation by flow cytometry.
Image caption:
Gating strategy to determine fractions of leucocytes (a–e).
(a) Viable, propidium iodide-negative cells were determined in FL3 versus SSC-A density plots.; (b) Single cells among viable cells were identified in FSC-A versus FSC-H density plots; (c) A region of granulocytes, lymphocytes, and monocytes were identified in FSC-A versus SSC-A density plots. Fractions of lymphoid cell subpopulations were determined after dual staining with antibodies specific for CD4 and CD8 or MHC II and CD21 to identify CD4+ and CD8+ lymphocytes (d) or CD21+ B-cells and CD21−MHC-II+ lymphocytes (e).
From: Lucassen A, Finkler-Schade C, Schuberth H-J. A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
Animals. 2021; 11(9):2726.
doi: 10.3390/ani11092726
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Horse CD8
- Product Type
- Monoclonal Antibody
- Clone
- CVS21
- Isotype
- IgG2a
- Specificity
- CD8
Antibody Quality and 
Unrivaled 
| Mouse anti Horse CD8 antibody, clone CVS21 recognizes the equine homolog of human CD8. Equine CD8 is expressed by a subset of T lymphocytes. In addition to the CVS21 clone, other CVS clones recognising equine MHC and cell surface antigens are available. |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% sodium azide (NaN3).
- Carrier Free
- Yes.
- Immunogen
- Equine peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml.
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
References for CD8 antibody
-
Ferreira-Dias, G. et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
Domest Anim Endocrinol. 29: 203-13. -
Krakowski, L. et al. (2017) Changes in blood lymphocyte subpopulations and expression of MHC-II molecules in wild mares before and after parturition
J Vet Res. 61 (2): 217-21. -
Witonsky, S. et al. (2019) Can levamisole upregulate the equine cell-mediated macrophage (M1) dendritic cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro.?
J Vet Intern Med. 33 (2): 889-96. -
Hillmann, A. et al. (2019) A novel direct co-culture assay analyzed by multicolor flow cytometry reveals context- and cell type-specific immunomodulatory effects of equine mesenchymal stromal cells.
PLoS One. 14 (6): e0218949. -
Tomlinson, J.E. et al. (2018) Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood.
Vet Immunol Immunopathol. 199: 22-31. -
Lucassen, A. et al. (2021) A Saccharomyces cerevisiae Fermentation Product (Olimond BB) Alters the Early Response after Influenza Vaccination in Racehorses.
Animals (Basel). 11(9):2726. -
Schauer, M. et al. (2018) Interaction of septin 7 and DOCK8 in equine lymphocytes reveals novel insights into signaling pathways associated with autoimmunity.
Sci Rep. 8 (1): 12332.
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