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CD5 antibody | CVS5

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Anti Horse CD5 Antibody, clone CVS5 thumbnail image 1 Anti Horse CD5 Antibody, clone CVS5 thumbnail image 2 Anti Horse CD5 Antibody, clone CVS5 thumbnail image 3 Anti Horse CD5 Antibody, clone CVS5 thumbnail image 4
Anti Horse CD5 Antibody, clone CVS5 gallery image 1
Staining of equine peripheral blood lymphocytes with Mouse anti Horse CD5:FITC (MCA1079F)
Anti Horse CD5 Antibody, clone CVS5 gallery image 2
Staining of equine peripheral blood lymphocytes with Mouse anti Equine CD5 (MCA1079GA), followed by Goat anti Mouse IgG:FITC (STAR117F)
Anti Horse CD5 Antibody, clone CVS5 gallery image 3
Staining of equine peripheral blood lymphocytes with Mouse anti Equine CD5:RPE(MCA1079PE)
Anti Horse CD5 Antibody, clone CVS5 gallery image 4
Figure A. RPE conjugated Mouse anti Horse CD4 (MCA1078PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Horse CD4 (MCA1078PE) and FITC conjugated Mouse anti Horse CD5 (MCA1079F). All experiments performed on red cell lysed horse blood gated on lymphocytes in the presence of 10% horse serum. Data acquired on the ZE5™ Cell Analyzer.

Mouse anti Horse CD5:FITC

Mouse anti Horse CD5

Mouse anti Horse CD5:RPE

Product Type
Monoclonal Antibody
Clone
CVS5
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1079F
Datasheet
F
SDS
0.1 mg loader

MCA1079GA
Datasheet
C F IP
SDS
0.1 mg loader

MCA1079PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Horse CD5 antibody, clone CVS5 recognizes the equine CD5 antigen, a single-pass type I membrane protein with an approximate molecular weight of 69 kDa. Equine CD5 is expressed on the majority of T-lymphocytes, in addition is has been reported that equine CD5 may also be detected at very low levels on B-cells and granulocyctes.

In addition to the CVS5 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.

Product Details

Target Species
Horse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Equine leucocytes.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse X63-Ag8.653 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for CD5 antibody

  1. Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets.
    Immunology. 74 (2): 251-7.
  2. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62 (2): 101-43.
  3. Moyo, N.A. et al. (2013) Differentiation and activation of equine monocyte-derived dendritic cells are not correlated with CD206 or CD83 expression.
    Immunology. 139 (4): 472-83.
  4. Mayall, S. et al. (2001) The anti-human CD21 antibody, BU33, identifies equine B cells.
    J Comp Pathol. 124 :83-7.
  5. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
    Vet Immunol Immunopathol. 60: 15-31.
  6. Colbath AC et al. (2016) Autologous and Allogeneic Equine Mesenchymal Stem Cells Exhibit Equivalent Immunomodulatory Properties In Vitro.
    Stem Cells Dev. Dec 13. [Epub ahead of print]
  7. Ziegler, A. et al. (2016) Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum.
    BMC Vet Res. 12 (1): 254.
  8. Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
    Dev Comp Immunol. 65: 352-7.

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