CD44 antibody | CVS18

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Anti Horse CD44 Antibody, clone CVS18 thumbnail image 1

Anti Horse CD44 Antibody, clone CVS18 thumbnail image 2

Anti Horse CD44 Antibody, clone CVS18 thumbnail image 3

Anti Horse CD44 Antibody, clone CVS18 thumbnail image 4

Anti Horse CD44 Antibody, clone CVS18 thumbnail image 5

Anti Horse CD44 Antibody, clone CVS18 thumbnail image 6

Anti Horse CD44 Antibody, clone CVS18 gallery image 1 — Staining of equine peripheral blood lymphocytes with Mouse anti Equine CD44 antibody, clone CVS18 (**MCA1082GA**), followed by FITC conjugated Goat anti Mouse IgG antibody (STAR117F)

Anti Horse CD44 Antibody, clone CVS18 gallery image 2 — Staining of equine peripheral blood lymphocytes with FITC conjugated Mouse anti Equine CD44 antibody, clone CVS18 (**MCA1082F**)

Anti Horse CD44 Antibody, clone CVS18 gallery image 3 — Staining of equine peripheral blood lymphocytes with FITC conjugated Mouse anti Equine CD44 antibody, clone CVS18 (**MCA1082F**)

Anti Horse CD44 Antibody, clone CVS18 gallery image 4 — **Published customer image:**
Mouse anti Horse CD44 antibody, clone CVS18 (**MCA1082GA**) used for evaluation of CD44 expressing white blood cells in equine samples by flow cytometry.
**Image caption:**
Antigen expression on WBC in CB, and CB-MSC at passage (p) 2, 3 and 5. Consistent gates were applied for all cell analyses. Control sample fluorescence is indicated by black lines, fluorescence of samples incubated with specific antibody by light blue/gray lines. Results were similar for samples from 5 animals. Numbers in each plot represent the mean proportion of cells expressing each antigen.

From: Tessier L, Bienzle D, Williams LB, Koch TG (2015)
Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells.
PLoS ONE 10(4): e0122954.

Anti Horse CD44 Antibody, clone CVS18 gallery image 5 — **Published customer image:**
Mouse anti Equine CD44 antibody, clone CVS18 (AHP1082GA) used for the immunohistochemical evaluation of CD44 expression on equine endometrial sections.
**Image caption:**
Immunohistochemistry of equine endometrial sections. Photomicrographs show localization of (a) MSC markers CD29, CD44, CD90, and CD105 and (b) perivascular markers NG2 and CD146 within the equine endometrium. DAPI was used to stain cell nuclei. Yellow arrows, endometrial glands; white arrows, blood vessels. DAPI 4′,6-diamidine-2′-phenylindole dihydrochloride

From: Rink BE, Amilon KR, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX.
Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 2017 Jul 12;8(1):166.

Anti Horse CD44 Antibody, clone CVS18 gallery image 6 — **Published customer image:**
Mouse anti Equine CD44 antibody, clone CVS18 (**MCA1082GA**) used for the evaluation of CD44expression on equine cells by flow cytometry.
**Image caption:**
Flow cytometry analysis. Representative flow cytometry histograms with percentages of endometrial and BM MSCs (n = 6 and n = 3 horses, respectively) positive for different MSC, perivascular, and hematopoietic cell surface markers. Grey areas, signal from isotype controls; black lines, signal from the specific cell surface marker. BM bone marrow, MSC mesenchymal stromal/stem cell

From: Rink BE, Amilon KR, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX.
Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 2017 Jul 12;8(1):166.

Mouse anti Horse CD44:FITC

Mouse anti Horse CD44

Mouse anti Horse CD44:RPE

Product Type: Monoclonal Antibody
Clone: CVS18
Isotype: IgG1

Product Code	Applications	Pack Size
MCA1082F	F	0.1 mg
MCA1082GA	C F	0.1 mg
MCA1082PE	F	100 Tests

Mouse anti Horse CD11a/CD18 antibody, clone CVS18 recognizes equine CD44, a plasma membrane glycoprotein broadly expressed on the cell surface of leucocytes. CD44 is the primary receptor for hyaluronate and functions in cell adhesion.

Equine CD44 is widely expressed and Mouse anti Horse CD11a/CD18 antibody, clone CVS18 may be used as a pan equine leucocyte marker.

Product Details

Target Species: Horse
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1.0 ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free: Yes
Immunogen: Equine leucocytes.
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC for one month or at -20^oC for longer.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC for one month or at -20^oC for longer.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.

More Information

UniProt: Q05078 Related reagents
Entrez Gene: CD44 Related reagents
GO Terms: GO:0007155 cell adhesion; GO:0016021 integral to membrane; GO:0004872 receptor activity; GO:0005540 hyaluronic acid binding
Regulatory: For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	1/25	1/200
Immunohistology - Frozen
Flow Cytometry	Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD44 antibody

Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
Vet Immunol Immunopathol. 42 (1): 3-60.
Rappocciolo,G. et al. (2003) Down-regulation of MHC class I expression by equine herpesvirus-1
J Gen Virol. 84: 293-300
De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
Cytometry A. 81: 312-23.
Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.
Stem Cells Dev. 19: 269-82.
Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
Cell Med. 4 (1): 1-11.
Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
BMC Vet Res. 11 (1): 361.
Maia L et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow.
Microsc Res Tech. 76 (6): 618-24.
Soboll, G. et al. (2003) Mucosal co-administration of cholera toxin and influenza virus hemagglutinin-DNA in ponies generates a local IgA response.
Vaccine. 21 (21-22): 3081-92.
Tessier L et al. (2015) Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.
PLoS One. 10 (4): e0122954.
Spaas, J.H. et al. (2015) Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants.
Cell Physiol Biochem. 37 (2): 651-665.
Gomiero, C. et al. (2016) Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology.
Vet Res Commun. 40 (1): 39-48.
Clark, K.C. et al. (2016) Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype.
Stem Cell Rev. 12 (2): 245-56.
Alvarenga, M.A. (2016) Feasibility and Safety of Endometrial Injection of Autologous Bone Marrow Mesenchymal Stem Cells in Mares
J Eq Vet Sci. 42: 12-8.
Lepage, S.I. et al. (2016) Generation, Characterization, and Multilineage Potency of Mesenchymal-Like Progenitors Derived from Equine Induced Pluripotent Stem Cells.
Stem Cells Dev. 25 (1): 80-9.
Maia, L. et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
Cell Biol Int. Nov 26. [Epub ahead of print]
Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
Front Immunol. 7: 392.
Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
Theriogenology. 100: 8-15.
Rink, B.E. et al. (2017) Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 8 (1): 166.
Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
BMC Vet Res. 11: 63.
SantosV, H.D. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
Res Vet Sci. 124: 38-45.