CD44 antibody | CVS18
Mouse anti Horse CD44 antibody, clone CVS18 (MCA1082GA) used for evaluation of CD44 expressing white blood cells in equine samples by flow cytometry.
Image caption:
Antigen expression on WBC in CB, and CB-MSC at passage (p) 2, 3 and 5. Consistent gates were applied for all cell analyses. Control sample fluorescence is indicated by black lines, fluorescence of samples incubated with specific antibody by light blue/gray lines. Results were similar for samples from 5 animals. Numbers in each plot represent the mean proportion of cells expressing each antigen.
From: Tessier L, Bienzle D, Williams LB, Koch TG (2015)
Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells.
PLoS ONE 10(4): e0122954.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082GA) used for the immunohistochemical evaluation of CD44 expression on equine endometrial sections.
Image caption:
Immunohistochemistry of equine endometrial sections. Photomicrographs show localization of (a) MSC markers CD29, CD44, CD90, and CD105 and (b) perivascular markers NG2 and CD146 within the equine endometrium. DAPI was used to stain cell nuclei. Yellow arrows, endometrial glands; white arrows, blood vessels. DAPI 4′,6-diamidine-2′-phenylindole dihydrochloride
From: Rink BE, Amilon KR, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX.
Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 2017 Jul 12;8(1):166.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082GA) used for the evaluation of CD44expression on equine cells by flow cytometry.
Image caption:
Flow cytometry analysis. Representative flow cytometry histograms with percentages of endometrial and BM MSCs (n = 6 and n = 3 horses, respectively) positive for different MSC, perivascular, and hematopoietic cell surface markers. Grey areas, signal from isotype controls; black lines, signal from the specific cell surface marker. BM bone marrow, MSC mesenchymal stromal/stem cell
From: Rink BE, Amilon KR, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX.
Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 2017 Jul 12;8(1):166.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082) used to identify equine mesenchymal stem cells by flow cytometry.
Image caption:
Representative image of equine ASC phenotype. Equine ASC phenotype panel. Positive markers: CD44, CD29, CD90, and MHC I. Negative markers: F6B and MHC II.
From: Barberini DJ, Aleman M, Aristizabal F, Spriet M, Clark KC, Walker NJ, Galuppo LD, Amorim RM, Woolard KD, Borjesson DL.
Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 2018 Apr 10;9(1):96.
doi: 10.1186/s13287-018-0849-6.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082GA) use for the evaluation of CD44 expression on adipose tissue-derived and bone marrow-derived mesenchymal stem cells by flow cytometry.
Image caption:
Flow cytometry histograms of AT-MSCs and BM-MSCs (A; upper and lower panels, respectively) showing positive staining of most cells for the MSC markers, CD29, CD44, CD90, and CD105 (dark line; ≥99% cells for all antibodies by detection of AF488 conjugate or FITC conjugate). Signal from isotype controls is shown by the gray peak and unstained curves were omitted for simplicity. (B) Results of quantitative polymerase chain reaction (qPCR) analysis of the MSC markers, CD44, CD73, CD90, and CD105, and (C) the endothelial markers, CD31 and CD144, and the hematopoietic marker, CD45, in AT-MSCs and BM-MSCs. All results are shown as mean ±SEM; n = 3 animals. *P <0.05 and **P <0.005. AU, arbitrary units; ND, not detected; SEM, standard error of the mean.
From: Esteves CL, Sheldrake TA, Dawson L, Menghini T, Rink BE, Amilon K, Khan N, Péault B, Donadeu FX.
Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
Stem Cells Dev. 2017 Jul 1;26(13):964-72.
doi: 10.1089/scd.2017.0017.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082GA) used to visualise CD44 expression on equine cell types by immunofluorescence.
Image caption:
Immunohistochemistry of pericyte, adventitial cell, and MSC markers in equine tissues. g, Colocalization (right panel) of the pericyte markers CD44 and CD146 (g) in adipose tissue. Individual staining is shown (left and middle panels). h, i Immunodetection of the adventitial cell marker CD34 (h) and the MSC marker CD44 (i) in the outer layer of blood vessels (arrows) in adipose tissue. 4′,6-Diamidino-2-phenylindole (DAPI) was used to stain cell nuclei. White scale bars = 10 μm
From: Esteves CL, Sheldrake TA, Mesquita SP, Pesántez JJ, Menghini T, Dawson L, Péault B, Donadeu FX.
Isolation and characterization of equine native MSC populations.
Stem Cell Res Ther. 2017 Apr 18;8(1):80.
doi: 10.1186/s13287-017-0525-2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Horse CD44 antibody, clone CVS18 (MCA1082GA) used to evaluate phenotype of equine synovial membrane derived mesenchymal stem cells by flow cytometry.
Image caption:
Results of flow cytometry using immunological markers on synovial membrane-derived mesenchymal stem cells. The grey area represents the negative control. The horizontal line in individual histograms indicates the population of the positive cells
From: Murata D, Ishikawa S, Sunaga T, Saito Y, Sogawa T, Nakayama K, Hobo S, Hatazoe T. Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses.
BMC Vet Res. 2022 Jan 22;18(1):53.
doi: 10.1186/s12917-021-03126-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Horse CD11a/CD18 antibody, clone CVS18 (MCA1082GA) used to label equine MSCs by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Immunohistochemistry of encapsulated MSCs. (A) Negative control of the sample; (B) Positive labelling of CD44 and C) Absence of labelling by MHC II. Harris’ hematoxylin counterstaining, 20x
From: de Souza JB, Rosa GDS, Rossi MC, Stievani FC, Pfeifer JPH, Krieck AMT, Bovolato ALC, Fonseca-Alves CE, Borrás VA, Alves ALG.
In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery.
Front Bioeng Biotechnol. 2021 Aug 27;9:674581.
doi: 10.3389/fbioe.2021.674581.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Horse CD44
- Product Type
- Monoclonal Antibody
- Clone
- CVS18
- Isotype
- IgG1
- Specificity
- CD44
Antibody Quality and Performance Guarantee
| Mouse anti Horse CD44 antibody, clone CVS18 recognizes equine CD44, a plasma membrane glycoprotein broadly expressed on the cell surface of leucocytes. CD44 is the primary receptor for hyaluronate and functions in cell adhesion. Mouse anti Horse CD44 antibody, clone CVS18 may be used as a pan equine leucocyte marker. |
- Target Species
- Horse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Equine leucocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD44 antibody
-
Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
Vet Immunol Immunopathol. 42 (1): 3-60. -
Rappocciolo,G. et al. (2003) Down-regulation of MHC class I expression by equine herpesvirus-1
J Gen Virol. 84: 293-300 -
De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
Cytometry A. 81: 312-23. -
Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.
Stem Cells Dev. 19: 269-82. -
Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
Cell Med. 4 (1): 1-11. -
Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
BMC Vet Res. 11 (1): 361. -
Maia L et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow.
Microsc Res Tech. 76 (6): 618-24. -
Soboll, G. et al. (2003) Mucosal co-administration of cholera toxin and influenza virus hemagglutinin-DNA in ponies generates a local IgA response.
Vaccine. 21 (21-22): 3081-92.
View The Latest Product References
-
Tessier, L. et al. (2015) Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.
PLoS One. 10 (4): e0122954. -
Spaas, J.H. et al. (2015) Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants.
Cell Physiol Biochem. 37 (2): 651-665. -
Gomiero, C. et al. (2016) Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology.
Vet Res Commun. 40 (1): 39-48. -
Clark, K.C. et al. (2016) Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype.
Stem Cell Rev. 12 (2): 245-56. -
Alvarenga, A.M. et al. (2016) Feasibility and Safety of Endometrial Injection of Autologous Bone Marrow Mesenchymal Stem Cells in Mares
J Eq Vet Sci. 42: 12-18. -
Lepage, S.I. et al. (2016) Generation, Characterization, and Multilineage Potency of Mesenchymal-Like Progenitors Derived from Equine Induced Pluripotent Stem Cells.
Stem Cells Dev. 25 (1): 80-9. -
Maia, L. et al. (2017) Conditioned medium: a new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
Cell Biol Int. 41 (3): 239-248. -
Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
Front Immunol. 7: 392. -
Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
Theriogenology. 100: 8-15. -
Rink, B.E. et al. (2017) Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 8 (1): 166. -
Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
BMC Vet Res. 11: 63. -
Dos Santos V, H. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
Res Vet Sci. 124: 38-45. -
Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 9 (1): 96. -
Esteves, C.L. et al. (2017) Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
Stem Cells Dev. 26 (13): 964-72. -
Esteves, C.L. et al. (2017) Isolation and characterization of equine native MSC populations.
Stem Cell Res Ther. 8 (1): 80. -
Tongu, E.A.O. et al. (2021) Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares.
Theriogenology. 169: 1-8. -
Murata, D. et al. (2022) Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses.
BMC Vet Res. 18 (1): 53. -
Bernardino, P.N. et al. (2022) Therapeutics prior to mesenchymal stromal cell therapy improves outcome in equine orthopedic injuries.
Am J Vet Res. 83 (10): ajvr.22.04.0072. -
Papa, P.M. et al. (2023) Intratesticular transplantation of allogenic mesenchymal stem cells mitigates testicular destruction after induced heat stress in Miniature-horse stallions.
J Equine Vet Sci. 132: 104961. -
Carvalho, A de M. et al. (2013) Isolation and characterization of equine peripheral blood-derived multipotent mesenchymal stromal cells
Pesquisa Veterinária Brasileira. 33 (9): 1151-4. -
Fülber, J. et al. (2021) Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system.
World J Stem Cells. 13 (6): 645-58. -
Cabezas, J. et al. (2020) In vitro preconditioning of equine adipose mesenchymal stem cells with prostaglandin E(2), substance P and their combination changes the cellular protein secretomics and improves their immunomodulatory competence without compromising stemness.
Vet Immunol Immunopathol. 228: 110100. -
de Souza, J.B. et al. (2021) In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery.
Front Bioeng Biotechnol. 9: 674581. -
Cassano, J.M. et al. (2023) Preliminary evaluation of safety and migration of immune activated mesenchymal stromal cells administered by subconjunctival injection for equine recurrent uveitis.
Front Vet Sci. 10: 1293199.
Further Reading
-
Burk, J. et al. (2013) Equine cellular therapy--from stall to bench to bedside?
Cytometry A. 83 (1): 103-13.
- Synonyms
- H-CAM
- PGP-1
- UniProt
- Q05078
- Entrez Gene
- CD44
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005540 hyaluronic acid binding
MCA1082GA
153314
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