CD44 antibody | CVS18
Mouse anti Horse CD44 antibody, clone CVS18 (MCA1082GA) used for evaluation of CD44 expressing white blood cells in equine samples by flow cytometry.
Image caption:
Antigen expression on WBC in CB, and CB-MSC at passage (p) 2, 3 and 5. Consistent gates were applied for all cell analyses. Control sample fluorescence is indicated by black lines, fluorescence of samples incubated with specific antibody by light blue/gray lines. Results were similar for samples from 5 animals. Numbers in each plot represent the mean proportion of cells expressing each antigen.
From: Tessier L, Bienzle D, Williams LB, Koch TG (2015)
Phenotypic and Immunomodulatory Properties of Equine Cord Blood-Derived Mesenchymal Stromal Cells.
PLoS ONE 10(4): e0122954.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082GA) used for the immunohistochemical evaluation of CD44 expression on equine endometrial sections.
Image caption:
Immunohistochemistry of equine endometrial sections. Photomicrographs show localization of (a) MSC markers CD29, CD44, CD90, and CD105 and (b) perivascular markers NG2 and CD146 within the equine endometrium. DAPI was used to stain cell nuclei. Yellow arrows, endometrial glands; white arrows, blood vessels. DAPI 4′,6-diamidine-2′-phenylindole dihydrochloride
From: Rink BE, Amilon KR, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX.
Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 2017 Jul 12;8(1):166.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082GA) used for the evaluation of CD44expression on equine cells by flow cytometry.
Image caption:
Flow cytometry analysis. Representative flow cytometry histograms with percentages of endometrial and BM MSCs (n = 6 and n = 3 horses, respectively) positive for different MSC, perivascular, and hematopoietic cell surface markers. Grey areas, signal from isotype controls; black lines, signal from the specific cell surface marker. BM bone marrow, MSC mesenchymal stromal/stem cell
From: Rink BE, Amilon KR, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX.
Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 2017 Jul 12;8(1):166.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082) used to identify equine mesenchymal stem cells by flow cytometry.
Image caption:
Representative image of equine ASC phenotype. Equine ASC phenotype panel. Positive markers: CD44, CD29, CD90, and MHC I. Negative markers: F6B and MHC II.
From: Barberini DJ, Aleman M, Aristizabal F, Spriet M, Clark KC, Walker NJ, Galuppo LD, Amorim RM, Woolard KD, Borjesson DL.
Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 2018 Apr 10;9(1):96.
doi: 10.1186/s13287-018-0849-6.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082GA) use for the evaluation of CD44 expression on adipose tissue-derived and bone marrow-derived mesenchymal stem cells by flow cytometry.
Image caption:
Flow cytometry histograms of AT-MSCs and BM-MSCs (A; upper and lower panels, respectively) showing positive staining of most cells for the MSC markers, CD29, CD44, CD90, and CD105 (dark line; ≥99% cells for all antibodies by detection of AF488 conjugate or FITC conjugate). Signal from isotype controls is shown by the gray peak and unstained curves were omitted for simplicity. (B) Results of quantitative polymerase chain reaction (qPCR) analysis of the MSC markers, CD44, CD73, CD90, and CD105, and (C) the endothelial markers, CD31 and CD144, and the hematopoietic marker, CD45, in AT-MSCs and BM-MSCs. All results are shown as mean ±SEM; n = 3 animals. *P <0.05 and **P <0.005. AU, arbitrary units; ND, not detected; SEM, standard error of the mean.
From: Esteves CL, Sheldrake TA, Dawson L, Menghini T, Rink BE, Amilon K, Khan N, Péault B, Donadeu FX.
Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
Stem Cells Dev. 2017 Jul 1;26(13):964-72.
doi: 10.1089/scd.2017.0017.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Equine CD44 antibody, clone CVS18 (MCA1082GA) used to visualise CD44 expression on equine cell types by immunofluorescence.
Image caption:
Immunohistochemistry of pericyte, adventitial cell, and MSC markers in equine tissues. g, Colocalization (right panel) of the pericyte markers CD44 and CD146 (g) in adipose tissue. Individual staining is shown (left and middle panels). h, i Immunodetection of the adventitial cell marker CD34 (h) and the MSC marker CD44 (i) in the outer layer of blood vessels (arrows) in adipose tissue. 4′,6-Diamidino-2-phenylindole (DAPI) was used to stain cell nuclei. White scale bars = 10 μm
From: Esteves CL, Sheldrake TA, Mesquita SP, Pesántez JJ, Menghini T, Dawson L, Péault B, Donadeu FX.
Isolation and characterization of equine native MSC populations.
Stem Cell Res Ther. 2017 Apr 18;8(1):80.
doi: 10.1186/s13287-017-0525-2.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Horse CD11a/CD18 antibody, clone CVS18 recognizes equine CD44, a plasma membrane glycoprotein broadly expressed on the cell surface of leucocytes. CD44 is the primary receptor for hyaluronate and functions in cell adhesion.
Equine CD44 is widely expressed and Mouse anti Horse CD11a/CD18 antibody, clone CVS18 may be used as a pan equine leucocyte marker.
Equine CD44 is widely expressed and Mouse anti Horse CD11a/CD18 antibody, clone CVS18 may be used as a pan equine leucocyte marker.
Product Details
- Target Species
- Horse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Equine leucocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q05078
- Entrez Gene
- CD44
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005540 hyaluronic acid binding
- Regulatory
- For research purposes only
Applications of CD44 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/25 | 1/200 | |
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for CD44 antibody
-
Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
Vet Immunol Immunopathol. 42 (1): 3-60. -
Rappocciolo,G. et al. (2003) Down-regulation of MHC class I expression by equine herpesvirus-1
J Gen Virol. 84: 293-300 -
De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
Cytometry A. 81: 312-23. -
Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.
Stem Cells Dev. 19: 269-82. -
Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
Cell Med. 4 (1): 1-11. -
Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
BMC Vet Res. 11 (1): 361. -
Maia L et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow.
Microsc Res Tech. 76 (6): 618-24. -
Soboll, G. et al. (2003) Mucosal co-administration of cholera toxin and influenza virus hemagglutinin-DNA in ponies generates a local IgA response.
Vaccine. 21 (21-22): 3081-92. -
Tessier, L. et al. (2015) Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.
PLoS One. 10 (4): e0122954. -
Spaas, J.H. et al. (2015) Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants.
Cell Physiol Biochem. 37 (2): 651-665. -
Gomiero, C. et al. (2016) Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology.
Vet Res Commun. 40 (1): 39-48. -
Clark, K.C. et al. (2016) Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype.
Stem Cell Rev. 12 (2): 245-56. -
Alvarenga, M.A. (2016) Feasibility and Safety of Endometrial Injection of Autologous Bone Marrow Mesenchymal Stem Cells in Mares
J Eq Vet Sci. 42: 12-8. -
Lepage, S.I. et al. (2016) Generation, Characterization, and Multilineage Potency of Mesenchymal-Like Progenitors Derived from Equine Induced Pluripotent Stem Cells.
Stem Cells Dev. 25 (1): 80-9. -
Maia, L. et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
Cell Biol Int. Nov 26. [Epub ahead of print] -
Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
Front Immunol. 7: 392. -
Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
Theriogenology. 100: 8-15. -
Rink, B.E. et al. (2017) Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 8 (1): 166. -
Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
BMC Vet Res. 11: 63. -
Dos Santos V, H. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
Res Vet Sci. 124: 38-45. -
Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 9 (1): 96. -
Esteves, C.L. et al. (2017) Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
Stem Cells Dev. 26 (13): 964-72. -
Esteves, C.L. et al. (2017) Isolation and characterization of equine native MSC populations.
Stem Cell Res Ther. 8 (1): 80. -
Tongu, E.A.O. et al. (2021) Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares.
Theriogenology. 169: 1-8.
Further Reading
-
Burk, J. et al. (2013) Equine cellular therapy--from stall to bench to bedside?
Cytometry A. 83 (1): 103-13.
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