CD44 antibody | CVS18

Mouse anti Horse CD44

Product Type
Monoclonal Antibody
Clone
CVS18
Isotype
IgG1
Specificity
CD44

Product Code Applications Pack Size List Price Your Price Qty
MCA1082GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Horse CD11a/CD18 antibody, clone CVS18 recognizes equine CD44, a plasma membrane glycoprotein broadly expressed on the cell surface of leucocytes. CD44 is the primary receptor for hyaluronate and functions in cell adhesion.

Equine CD44 is widely expressed and Mouse anti Horse CD11a/CD18 antibody, clone CVS18 may be used as a pan equine leucocyte marker.

Target Species
Horse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Equine leucocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD44 antibody

  1. Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
    Vet Immunol Immunopathol. 42 (1): 3-60.
  2. Rappocciolo,G. et al. (2003) Down-regulation of MHC class I expression by equine herpesvirus-1
    J Gen Virol. 84: 293-300
  3. De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
    Cytometry A. 81: 312-23.
  4. Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.
    Stem Cells Dev. 19: 269-82.
  5. Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
    Cell Med. 4 (1): 1-11.
  6. Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
    BMC Vet Res. 11 (1): 361.
  7. Maia L et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow.
    Microsc Res Tech. 76 (6): 618-24.
  8. Soboll, G. et al. (2003) Mucosal co-administration of cholera toxin and influenza virus hemagglutinin-DNA in ponies generates a local IgA response.
    Vaccine. 21 (21-22): 3081-92.
  9. View The Latest Product References
  10. Tessier, L. et al. (2015) Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.
    PLoS One. 10 (4): e0122954.
  11. Spaas, J.H. et al. (2015) Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants.
    Cell Physiol Biochem. 37 (2): 651-665.
  12. Gomiero, C. et al. (2016) Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology.
    Vet Res Commun. 40 (1): 39-48.
  13. Clark, K.C. et al. (2016) Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype.
    Stem Cell Rev. 12 (2): 245-56.
  14. Alvarenga, M.A. (2016) Feasibility and Safety of Endometrial Injection of Autologous Bone Marrow Mesenchymal Stem Cells in Mares
    J Eq Vet Sci. 42: 12-8.
  15. Lepage, S.I. et al. (2016) Generation, Characterization, and Multilineage Potency of Mesenchymal-Like Progenitors Derived from Equine Induced Pluripotent Stem Cells.
    Stem Cells Dev. 25 (1): 80-9.
  16. Maia, L. et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
    Cell Biol Int. Nov 26. [Epub ahead of print]
  17. Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
    Front Immunol. 7: 392.
  18. Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
    Theriogenology. 100: 8-15.
  19. Rink, B.E. et al. (2017) Isolation and characterization of equine endometrial mesenchymal stromal cells.
    Stem Cell Res Ther. 8 (1): 166.
  20. Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
    BMC Vet Res. 11: 63.
  21. Dos Santos V, H. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
    Res Vet Sci. 124: 38-45.
  22. Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
    Stem Cell Res Ther. 9 (1): 96.
  23. Esteves, C.L. et al. (2017) Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
    Stem Cells Dev. 26 (13): 964-72.
  24. Esteves, C.L. et al. (2017) Isolation and characterization of equine native MSC populations.
    Stem Cell Res Ther. 8 (1): 80.
  25. Tongu, E.A.O. et al. (2021) Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares.
    Theriogenology. 169: 1-8.
  26. Murata, D. et al. (2022) Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses.
    BMC Vet Res. 18 (1): 53.
  27. Bernardino, P.N. et al. (2022) Therapeutics prior to mesenchymal stromal cell therapy improves outcome in equine orthopedic injuries.
    Am J Vet Res. 83 (10): ajvr.22.04.0072.

Further Reading

  1. Burk, J. et al. (2013) Equine cellular therapy--from stall to bench to bedside?
    Cytometry A. 83 (1): 103-13.

Flow Cytometry

Synonyms
H-CAM
PGP-1
UniProt
Q05078
Entrez Gene
CD44
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005540 hyaluronic acid binding
View more products with CD44 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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