CD44 antibody | CVS18









Equine CD44 is widely expressed and Mouse anti Horse CD11a/CD18 antibody, clone CVS18 may be used as a pan equine leucocyte marker.
Product Details
- Target Species
- Horse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Equine leucocytes.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q05078
- Entrez Gene
- CD44
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005540 hyaluronic acid binding
- Regulatory
- For research purposes only
Applications of CD44 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 | |
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen | |||
Flow Cytometry | Neat |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Product Specific References
References for CD44 antibody
-
Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
Vet Immunol Immunopathol. 42 (1): 3-60. -
Rappocciolo,G. et al. (2003) Down-regulation of MHC class I expression by equine herpesvirus-1
J Gen Virol. 84: 293-300 -
De Schauwer, C. et al. (2012) In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.
Cytometry A. 81: 312-23. -
Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.
Stem Cells Dev. 19: 269-82. -
Carrade, D.D. et al. (2012) Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().
Cell Med. 4 (1): 1-11. -
Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
BMC Vet Res. 11 (1): 361. -
Maia L et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow.
Microsc Res Tech. 76 (6): 618-24. -
Soboll, G. et al. (2003) Mucosal co-administration of cholera toxin and influenza virus hemagglutinin-DNA in ponies generates a local IgA response.
Vaccine. 21 (21-22): 3081-92. -
Tessier, L. et al. (2015) Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.
PLoS One. 10 (4): e0122954. -
Spaas, J.H. et al. (2015) Chondrogenic Priming at Reduced Cell Density Enhances Cartilage Adhesion of Equine Allogeneic MSCs - a Loading Sensitive Phenomenon in an Organ Culture Study with 180 Explants.
Cell Physiol Biochem. 37 (2): 651-665. -
Gomiero, C. et al. (2016) Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology.
Vet Res Commun. 40 (1): 39-48. -
Clark, K.C. et al. (2016) Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype.
Stem Cell Rev. 12 (2): 245-56. -
Alvarenga, M.A. (2016) Feasibility and Safety of Endometrial Injection of Autologous Bone Marrow Mesenchymal Stem Cells in Mares
J Eq Vet Sci. 42: 12-8. -
Lepage, S.I. et al. (2016) Generation, Characterization, and Multilineage Potency of Mesenchymal-Like Progenitors Derived from Equine Induced Pluripotent Stem Cells.
Stem Cells Dev. 25 (1): 80-9. -
Maia, L. et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
Cell Biol Int. Nov 26. [Epub ahead of print] -
Maumus, M. et al. (2016) Utility of a Mouse Model of Osteoarthritis to Demonstrate Cartilage Protection by IFNγ-Primed Equine Mesenchymal Stem Cells.
Front Immunol. 7: 392. -
Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
Theriogenology. 100: 8-15. -
Rink, B.E. et al. (2017) Isolation and characterization of equine endometrial mesenchymal stromal cells.
Stem Cell Res Ther. 8 (1): 166. -
Maia, L. et al. (2015) Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.
BMC Vet Res. 11: 63. -
SantosV, H.D. et al. (2019) Evaluation of alginate hydrogel encapsulated mesenchymal stem cell migration in horses.
Res Vet Sci. 124: 38-45. -
Barberini, D.J. et al. (2018) Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.
Stem Cell Res Ther. 9 (1): 96. -
Esteves, C.L. et al. (2017) Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype.
Stem Cells Dev. 26 (13): 964-72. -
Esteves, C.L. et al. (2017) Isolation and characterization of equine native MSC populations.
Stem Cell Res Ther. 8 (1): 80.
Further Reading
-
Burk, J. et al. (2013) Equine cellular therapy--from stall to bench to bedside?
Cytometry A. 83 (1): 103-13.
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