CD13 antibody | CVS19

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Anti Horse CD13 Antibody, clone CVS19 thumbnail image 1

Anti Horse CD13 Antibody, clone CVS19 thumbnail image 2

Anti Horse CD13 Antibody, clone CVS19 thumbnail image 3

Anti Horse CD13 Antibody, clone CVS19 gallery image 1 — Staining of equine peripheral blood granulocytes with Mouse anti Horse CD13 (**MCA1084GA**) followed by Goat anti Mouse IgG:FITC (STAR117F)

Anti Horse CD13 Antibody, clone CVS19 gallery image 2 — Staining of equine peripheral blood granulocytes with Mouse anti Horse CD13:FITC (**MCA1084F**)

Anti Horse CD13 Antibody, clone CVS19 gallery image 3 — Staining of equine peripheral blood granulocytes with Mouse anti Horse CD13:RPE(**MCA1084PE**)

Mouse anti Horse CD13:FITC

Mouse anti Horse CD13

Mouse anti Horse CD13:RPE

Product Type: Monoclonal Antibody
Clone: CVS19
Isotype: IgG1

Product Code	Applications	Pack Size
MCA1084F	F	0.1 mg
MCA1084GA	C F IP	0.1 mg
MCA1084PE	F	100 Tests

Mouse anti Horse CD13 antibody, clone CVS19 recognizes the equine CD13 cell surface antigen, also known as Aminopeptidase N, a single-pass type II membrane protein belonging to the peptidase N family with a molecular weight of between 150 - 170 kDa.

CD13 is widely expressed by a range of cell types including all blood neutrophils, basophils, monocytes, fibroblasts, kidney epithelial cells, endothelial cells and mesenchymal stem cells, but not by T or B cells. It is involved in a broad spectrum of biological processes and is believed to be linked to a number of disease states including tumor invasion (Saiki et al. 1993).

Mouse anti Horse CD13 antibody, clone CVS19 may be used for differentiating myeloid and lymphoid linage cells in tumors of the haematopoietic system.

In addition to clone CVS19, other CVS clones recognising equine MHC and cell surface antigens are available.

Product Details

Target Species: Horse
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1.0ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein G
Preparation: Purified IgG prepared by affinity chromatography on Protein G
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free: Yes
Immunogen: Equine leucocytes.
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised mice were fused with cells of the mouse X63-Ag8.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC for one month or at -20^oC for longer.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.

More Information

Regulatory: For research purposes only

Applications of CD13 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry	1/25	1/200
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry		Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for CD13 antibody

Kydd, J.H. & Antczak, D.F. (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991
Equine Immunology 4: 5.
Dunkel, B. et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation.
Vet Immunol Immunopathol. 131: 25-32.
Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62: 101-143
Loftus J.P et al. (2006) Matrix metalloproteinase-9 in laminae of black walnut extract treated horses correlates with neutrophil abundance.
Vet Immunol Immunopathol. 113: 267-76.
Aalberts, M. et al. (2012) Spermatozoa recruit prostasomes in response to capacitation induction.
Biochim Biophys Acta.1834: 2326-35.
Maia, L. et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow.
Microsc Res Tech. 76: 618-24.
Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.
Stem Cells Dev. 19: 269-82.
Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
Dev Comp Immunol. 65: 352-7.

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