CD13 antibody | CVS19

Mouse anti Horse CD13

Product Type
Monoclonal Antibody
Clone
CVS19
Isotype
IgG1
Specificity
CD13

Product Code Applications Pack Size List Price Your Price Qty
MCA1084GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
List Price Your Price
loader
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Mouse anti Horse CD13 antibody, clone CVS19 recognizes the equine CD13 cell surface antigen, also known as Aminopeptidase N, a single-pass type II membrane protein belonging to the peptidase N family with a molecular weight of between 150 - 170 kDa.

CD13 is widely expressed by a range of cell types including all blood neutrophils, basophils, monocytes, fibroblasts, kidney epithelial cells, endothelial cells and mesenchymal stem cells, but not by T or B cells. It is involved in a broad spectrum of biological processes and is believed to be linked to a number of disease states including tumor invasion (Saiki et al. 1993).

Mouse anti Horse CD13 antibody, clone CVS19 may be used for differentiating myeloid and lymphoid linage cells in tumors of the haematopoietic system.

In addition to clone CVS19, other CVS clones recognising equine MHC and cell surface antigens are available.

Target Species
Horse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Equine leucocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for CD13 antibody

  1. Kydd, J.H. & Antczak, D.F. (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991
    Equine Immunology 4: 5.
  2. Dunkel, B. et al. (2009) Neutrophil and platelet activation in equine recurrent airway obstruction is associated with increased neutrophil CD13 expression, but not platelet CD41/61 and CD62P or neutrophil-platelet aggregate formation.
    Vet Immunol Immunopathol. 131: 25-32.
  3. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
    Vet Immunol Immunopathol. 62: 101-143
  4. Loftus J.P et al. (2006) Matrix metalloproteinase-9 in laminae of black walnut extract treated horses correlates with neutrophil abundance.
    Vet Immunol Immunopathol. 113: 267-76.
  5. Aalberts, M. et al. (2012) Spermatozoa recruit prostasomes in response to capacitation induction.
    Biochim Biophys Acta.1834: 2326-35.
  6. Maia, L. et al. (2013) Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow.
    Microsc Res Tech. 76: 618-24.
  7. Radcliffe, C.H. et al. (2010) Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations.
    Stem Cells Dev. 19: 269-82.
  8. Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
    Dev Comp Immunol. 65: 352-7.
  9. View The Latest Product References
  10. Soboll Hussey, G. et al. (2011) Evaluation of immune responses following infection of ponies with an EHV-1 ORF1/2 deletion mutant.
    Vet Res. 42: 23.

Synonyms
Aminopeptidase N
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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