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Lymphocytes antibody | MsGp2

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Anti Guinea Pig Lymphocytes and Langerhans Cells Antibody, clone MsGp2 thumbnail image 1 Anti Guinea Pig Lymphocytes and Langerhans Cells Antibody, clone MsGp2 thumbnail image 2
Anti Guinea Pig Lymphocytes and Langerhans Cells Antibody, clone MsGp2 gallery image 1
Staining of Guinea Pig peripheral blood lymphocytes with Mouse anti Guinea Pig Lymphocytes and Langerhans Cells (MCA560GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)
Anti Guinea Pig Lymphocytes and Langerhans Cells Antibody, clone MsGp2 gallery image 2
Published customer image:
Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 (MCA560) used for the detection of infiltrating lymphocytes in guinea pig skin following intradermal Mantoux injection and subsequent electroporation by immunofluorescence.
Image caption:
Histological analysis reveals infiltration of lymphocytes at the EP treatment site. Guinea pig skin sections were immunohistochemically stained with an antibody recognizing lymphocytes and Langerhans cells (Tetramethylrhodamine isothiocyanate (TRITC) secondary) after ID GFP plasmid administration followed by EP with SEP. Skin biopsies were removed, cryosectioned, antibody and 4',6-diamidino-2-phenylindole (DAPI) stained and visualized under fluorescence microscopy at 20× and 40×. An untreated control (no DNA, no EP) is also shown.

From: Mendoza, J.M.; Amante, D.H.; Kichaev, G.; Knott, C.L.; Kiosses, W.B.; Smith, T.R.F.; Sardesai, N.Y.; Broderick, K.E.
Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation.
Vaccines 2013, 1, 384-97.

Mouse anti Guinea Pig Lymphocytes and Langerhans Cells

Product Type
Monoclonal Antibody
Clone
MsGp2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA560GA
Datasheet
C F IF
SDS
0.1 mg loader

Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 recognizes an antigen present on the majority of T and B lymphocytes and thymocytes, although the antigen is not expressed by germinal centre cells of the lymph node (Kraal et al. 1986).

Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 also reacts strongly with epidermal Langerhans cells, giving a superior profile than anti-MHC Class II antibodies on these cells (Healey et al. 1987).

Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 has the same distribution as Mouse anti Guinea Pig CD90 antibody, clone CT4 which detects an antigen reportedly involved in the organ non-specific trafficking of guinea pig lymphocytes (Kraal et al 1988). However, unlike clone CT4, clone MsGp2 does not functionally inhibit lymphocyte adherence to high endothelial venules in vitro (Healey et al. 1987).

Product Details

Target Species
Guinea Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Nylon wool adherent B cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Lymphocytes antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunofluorescence
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Guinea Pig T Lymphocytes MCA564GA C F 0.1 mg loader

Product Specific References

References for Lymphocytes antibody

  1. Healey, D.G. et al. (1987) An antigenic determinant common to lymphocytes and Langerhans cells of the guinea pig.
    Int Arch Allergy Appl Immunol. 82 (2): 120-4.
  2. Kraal, G. et al. (1986) A surface molecule on guinea pig lymphocytes involved in adhesion and homing.
    Eur J Immunol. 16 (12): 1515-9.
  3. Baker, D. et al. (1987) Induction of sensitization and tolerance in contact sensitivity with haptenated epidermal cells in the guinea-pig.
    Immunology. 62 (4): 659-64.
  4. Mendoza, J.M. et al. (2013) Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation.
    Vaccines (Basel). 1 (3): 384-97.
  5. Smith, T.R. et al. (2014) DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response.
    Mol Ther Methods Clin Dev. 1: 14054.
  6. Sato H & Kamiya H (1995) Role of epidermal Langerhans' cells in the induction of protective immunity to Schistosoma mansoni in guinea-pigs.
    Immunology. 84 (2): 233-40.
  7. Schäfer, H. et al. (1991) T cell proliferation induced by monoclonal antibodies to a phosphatidylinositol-linked differentiation antigen of guinea pig lymphocytes.
    Eur J Immunol. 21 (3): 701-5.
  8. Schäfer, H. & Burger, R. (2012) Tools for cellular immunology and vaccine research the in the guinea pig: monoclonal antibodies to cell surface antigens and cell lines.
    Vaccine. 30 (40): 5804-11.

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