Lymphocytes antibody | MsGp2
Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 (MCA560) used for the detection of infiltrating lymphocytes in guinea pig skin following intradermal Mantoux injection and subsequent electroporation by immunofluorescence.
Image caption:
Histological analysis reveals infiltration of lymphocytes at the EP treatment site. Guinea pig skin sections were immunohistochemically stained with an antibody recognizing lymphocytes and Langerhans cells (Tetramethylrhodamine isothiocyanate (TRITC) secondary) after ID GFP plasmid administration followed by EP with SEP. Skin biopsies were removed, cryosectioned, antibody and 4',6-diamidino-2-phenylindole (DAPI) stained and visualized under fluorescence microscopy at 20× and 40×. An untreated control (no DNA, no EP) is also shown.
From: Mendoza, J.M.; Amante, D.H.; Kichaev, G.; Knott, C.L.; Kiosses, W.B.; Smith, T.R.F.; Sardesai, N.Y.; Broderick, K.E.
Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation.
Vaccines 2013, 1, 384-97.
Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 recognizes an antigen present on the majority of T and B lymphocytes and thymocytes, although the antigen is not expressed by germinal centre cells of the lymph node (Kraal et al 1986).
Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 also reacts strongly with epidermal Langerhans cells, giving a superior profile than anti-MHC Class II antibodies on these cells (Healey et al. 1987).
Clone MsGp2 has the same distribution as Mouse anti Guinea Pig CD90 antibody, clone CT4 which detects an antigen reportedly involved in the organ non-specific trafficking of guinea pig lymphocytes (Kraal et al 1988). However, unlike clone CT4, clone MsGp2 does not functionally inhibit lymphocyte adherence to high endothelial venules in vitro (Healey et al. 1987).
Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 also reacts strongly with epidermal Langerhans cells, giving a superior profile than anti-MHC Class II antibodies on these cells (Healey et al. 1987).
Clone MsGp2 has the same distribution as Mouse anti Guinea Pig CD90 antibody, clone CT4 which detects an antigen reportedly involved in the organ non-specific trafficking of guinea pig lymphocytes (Kraal et al 1988). However, unlike clone CT4, clone MsGp2 does not functionally inhibit lymphocyte adherence to high endothelial venules in vitro (Healey et al. 1987).
Product Details
- Target Species
- Guinea Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Nylon wool adherent B cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Lymphocytes antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 | |
| Immunofluorescence | |||
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Guinea Pig T Lymphocytes | MCA564GA | 0.1 mg | C F |
Product Specific References
References for Lymphocytes antibody
-
Healey, D.G. et al. (1987) An antigenic determinant common to lymphocytes and Langerhans cells of the guinea pig.
Int Arch Allergy Appl Immunol. 82 (2): 120-4. -
Kraal, G. et al. (1986) A surface molecule on guinea pig lymphocytes involved in adhesion and homing.
Eur J Immunol. 16 (12): 1515-9. -
Baker, D. et al. (1987) Induction of sensitization and tolerance in contact sensitivity with haptenated epidermal cells in the guinea-pig.
Immunology. 62 (4): 659-64. -
Mendoza, J.M. et al. (2013) Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation.
Vaccines (Basel). 1 (3): 384-97. -
Smith, T.R. et al. (2014) DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response.
Mol Ther Methods Clin Dev. 1: 14054. -
Sato H & Kamiya H (1995) Role of epidermal Langerhans' cells in the induction of protective immunity to Schistosoma mansoni in guinea-pigs.
Immunology. 84 (2): 233-40. -
Schäfer, H. et al. (1991) T cell proliferation induced by monoclonal antibodies to a phosphatidylinositol-linked differentiation antigen of guinea pig lymphocytes.
Eur J Immunol. 21 (3): 701-5. -
Schäfer, H. & Burger, R. (2012) Tools for cellular immunology and vaccine research the in the guinea pig: monoclonal antibodies to cell surface antigens and cell lines.
Vaccine. 30 (40): 5804-11.
