Lymphocytes antibody | MsGp2
Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 (MCA560) used for the detection of infiltrating lymphocytes in guinea pig skin following intradermal Mantoux injection and subsequent electroporation by immunofluorescence.
Image caption:
Histological analysis reveals infiltration of lymphocytes at the EP treatment site. Guinea pig skin sections were immunohistochemically stained with an antibody recognizing lymphocytes and Langerhans cells (Tetramethylrhodamine isothiocyanate (TRITC) secondary) after ID GFP plasmid administration followed by EP with SEP. Skin biopsies were removed, cryosectioned, antibody and 4',6-diamidino-2-phenylindole (DAPI) stained and visualized under fluorescence microscopy at 20× and 40×. An untreated control (no DNA, no EP) is also shown.
From: Mendoza, J.M.; Amante, D.H.; Kichaev, G.; Knott, C.L.; Kiosses, W.B.; Smith, T.R.F.; Sardesai, N.Y.; Broderick, K.E.
Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation.
Vaccines 2013, 1, 384-97.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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- Product Type
- Monoclonal Antibody
- Clone
- MsGp2
- Isotype
- IgG1
- Specificity
- Lymphocytes
Antibody Quality and Performance Guarantee
| Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 recognizes an antigen present on the majority of T and B lymphocytes and thymocytes, although the antigen is not expressed by germinal centre cells of the lymph node (Kraal et al. 1986). Mouse anti Guinea Pig lymphocytes and Langerhans cells antibody, clone MsGp2 also reacts strongly with epidermal Langerhans cells, giving a superior profile than anti-MHC Class II antibodies on these cells (Healey et al. 1987). Clone MsGp2 does not functionally inhibit lymphocyte adherence to high endothelial venules in vitro (Healey et al. 1987). |
- Target Species
- Guinea Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Nylon wool adherent B cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Guinea Pig T Lymphocytes | MCA564GA | C F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Guinea Pig T Lymphocytes | ||||||
Source Reference
-
Healey, D.G. et al. (1987) An antigenic determinant common to lymphocytes and Langerhans cells of the guinea pig.
Int Arch Allergy Appl Immunol. 82 (2): 120-4.
References for Lymphocytes antibody
-
Baker, D. et al. (1987) Induction of sensitization and tolerance in contact sensitivity with haptenated epidermal cells in the guinea-pig.
Immunology. 62 (4): 659-64. -
Schäfer, H. et al. (1991) T cell proliferation induced by monoclonal antibodies to a phosphatidylinositol-linked differentiation antigen of guinea pig lymphocytes.
Eur J Immunol. 21 (3): 701-5. -
Sato H & Kamiya H (1995) Role of epidermal Langerhans' cells in the induction of protective immunity to Schistosoma mansoni in guinea-pigs.
Immunology. 84 (2): 233-40. -
Schäfer, H. & Burger, R. (2012) Tools for cellular immunology and vaccine research the in the guinea pig: monoclonal antibodies to cell surface antigens and cell lines.
Vaccine. 30 (40): 5804-11. -
Mendoza, J.M. et al. (2013) Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation.
Vaccines (Basel). 1 (3): 384-97. -
Smith, T.R. et al. (2014) DNA vaccination strategy targets epidermal dendritic cells, initiating their migration and induction of a host immune response.
Mol Ther Methods Clin Dev. 1: 14054.
MCA560GA
0215
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