IgE antibody | E6-71A1
Biotin conjugated Mouse anti Canine IgE antibody, clone E6-71-A1 (MCA1896B) used for the detection of canine IgE in sera on two dimensional immunoblots
Image caption:
A – IgE immunoblot from D. glomerata 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase anti-dog-IgE. Dog D. glomerata allergogram with a pool of sera. B – Two-dimensional diagram from dog D. glomerata allergogram in Figure 3 A. Recognized allergen spots are identified from a to n
From: Martins LM, Marques AG, Pereira LM, Semião-Santos SJ, Bento OP.
Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Postepy Dermatol Alergol. 2017 Feb;34(1):60-9.
Biotin conjugated Mouse anti Canine IgE antibody, clone E6-71-A1 (MCA1896B) used for the detection of canine IgE in sera on two dimensional immunoblots
Image caption:
A – IgE immunoblot from P. pratense 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase antidog-IgE. Dog P. pratense allergogram with a pool of sera. B – Two-dimensional diagram from dog P. pratense allergogram in Figure 4 A. Recognized allergen spots are identified from a to z7
From: Martins LM, Marques AG, Pereira LM, Semião-Santos SJ, Bento OP.
Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Postepy Dermatol Alergol. 2017 Feb;34(1):60-9.
Mouse anti Dog IgE antibody, clone E6-71A1 recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of ~62-65 kDa under reducing conditions.
Product Details
- Target Species
- Dog
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.05% Sodium Azide - Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
- Immunogen
- Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of IgE antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting | |||
| ELISA | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- The antibody may be used as a detection antibody in a sandwich ELISA in combination with MCA1895 as the capture antibody.
- Western Blotting
- Under reducing conditions MCA1896B detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE.
- Western Blotting
- Under reducing conditions MCA1896 detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for IgE antibody
-
Ognjenovic, J. et al. (2013) Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis.
Vet Immunol Immunopathol. 155: 38-47. -
Martins, L.M. et al. (2017) Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Postepy Dermatol Alergol. 34 (1): 60-9.
