IgE antibody | E6-71A1
Biotin conjugated Mouse anti Canine IgE antibody, clone E6-71-A1 (MCA1896B) used for the detection of canine IgE in sera on two dimensional immunoblots
Image caption:
A – IgE immunoblot from D. glomerata 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase anti-dog-IgE. Dog D. glomerata allergogram with a pool of sera. B – Two-dimensional diagram from dog D. glomerata allergogram in Figure 3 A. Recognized allergen spots are identified from a to n
From: Martins LM, Marques AG, Pereira LM, Semião-Santos SJ, Bento OP.
Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Postepy Dermatol Alergol. 2017 Feb;34(1):60-9.
Biotin conjugated Mouse anti Canine IgE antibody, clone E6-71-A1 (MCA1896B) used for the detection of canine IgE in sera on two dimensional immunoblots
Image caption:
A – IgE immunoblot from P. pratense 2-D separation, revealed with biotin-ExtrAvidin-alkaline phosphatase antidog-IgE. Dog P. pratense allergogram with a pool of sera. B – Two-dimensional diagram from dog P. pratense allergogram in Figure 4 A. Recognized allergen spots are identified from a to z7
From: Martins LM, Marques AG, Pereira LM, Semião-Santos SJ, Bento OP.
Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Postepy Dermatol Alergol. 2017 Feb;34(1):60-9.
Mouse anti Dog IgE antibody, clone E6-71A1 recognises canine IgE. E6-2A1 does not cross react with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of ~62-65 kDa under reducing conditions.
Product Details
- Target Species
- Dog
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgE antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting | |||
| ELISA | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- The antibody may be used as a detection antibody in a sandwich ELISA in combination with MCA1895 as the capture antibody.
- Western Blotting
- Under reducing conditions MCA1896B detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE.
- Western Blotting
- Under reducing conditions MCA1896 detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE.
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Secondary Antibodies Available
Product Specific References
References for IgE antibody
-
Ognjenovic, J. et al. (2013) Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis.
Vet Immunol Immunopathol. 155: 38-47. -
Martins, L.M. et al. (2017) Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Postepy Dermatol Alergol. 34 (1): 60-9.
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