IgE antibody | E6-71A1


Product Details
- Target Species
- Dog
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.05% Sodium Azide - Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
- Immunogen
- Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of IgE antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Western Blotting | |||
ELISA | |||
Western Blotting |
- ELISA
- The antibody may be used as a detection antibody in a sandwich ELISA in combination with MCA1895 as the capture antibody.
- Western Blotting
- Under reducing conditions MCA1896B detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE.
- Western Blotting
- Under reducing conditions MCA1896 detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE.
Secondary Antibodies Available
Product Specific References
References for IgE antibody
-
Ognjenovic, J. et al. (2013) Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis.
Vet Immunol Immunopathol. 155: 38-47. -
Martins, L.M. et al. (2017) Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting.
Postepy Dermatol Alergol. 34 (1): 60-9.