CD4 antibody | CA13.1E4
Mouse anti Dog CD4 antibody (CA13.1E4), a monoclonal antibody specific for canine CD4. CA13.1E4 was clustered at the first Canine Leukocyte Antigen Workshop (Claw) [Cobbold et al. 1992 ] and identifies, by immunoprecipitation a ~60 kDa monomeric protein under both reducing and non-reducing conditions. Canine CD4 is a surface glycoprotein expressed by non CD8 expressing T lymphocytes, with similar developmental and functional characteristics to T helper cells described in other mammalian species. Mouse anti Dog CD4 also recognizes a population of CD8 positive cells in the canine thymus (Moore et al. 1992).
CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8 (Bismarck et al. 2012 ). Similar reports have been made for cynomolgus monkey (Nam et al. 2000), pig (Saalmuller et al. 1987 , Pescowitz et al. 1990) as well as rat, chicken and human (reviewed Zuckermann 1999 ).
Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species (Moore et al. 1992).
Clone CA13.1E4 also demonstrates immunohistological staining of splenic marginal zone macrophages (Moore et al. 1992). Other macrophage populations, such as splenic red pulp macrophages and Langerhans cells do not stain with clone CA13.1E4. Canine CD4 positive T cells can be further characterized according to their simultaneous expression of CD45RA as recognized by Rat anti Canine anti CD45RA clone CA4.1D3 in a manner analogous to that seen with human T cells (Moore et al. 1992).
Clone CA13.1E4 has been used amongst a large panel of anti-canine monoclonal antibodies for the study of various lymphoproliferative diseases. T lymphocytes in large granular lymphocyte (LGL) lymphocytosis are negative for CD4 (McDonough and Moore 2000 ). Dogs with chronic myelogenous leukemia demonstrate CD4 positive staining on neutrophils (Tarrant et al. 2001). CD4 expression varies considerably between various canine leukemias.
CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8 (Bismarck et al. 2012 ). Similar reports have been made for cynomolgus monkey (Nam et al. 2000), pig (Saalmuller et al. 1987 , Pescowitz et al. 1990) as well as rat, chicken and human (reviewed Zuckermann 1999 ).
Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species (Moore et al. 1992).
Clone CA13.1E4 also demonstrates immunohistological staining of splenic marginal zone macrophages (Moore et al. 1992). Other macrophage populations, such as splenic red pulp macrophages and Langerhans cells do not stain with clone CA13.1E4. Canine CD4 positive T cells can be further characterized according to their simultaneous expression of CD45RA as recognized by Rat anti Canine anti CD45RA clone CA4.1D3 in a manner analogous to that seen with human T cells (Moore et al. 1992).
Clone CA13.1E4 has been used amongst a large panel of anti-canine monoclonal antibodies for the study of various lymphoproliferative diseases. T lymphocytes in large granular lymphocyte (LGL) lymphocytosis are negative for CD4 (McDonough and Moore 2000 ). Dogs with chronic myelogenous leukemia demonstrate CD4 positive staining on neutrophils (Tarrant et al. 2001). CD4 expression varies considerably between various canine leukemias.
Product Details
- Target Species
- Dog
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Canine Thymocytes
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the P3X63-Ag.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P33705 Related reagents
- Entrez Gene
- CD4 Related reagents
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0045058 T cell selection
- Regulatory
- For research purposes only
Applications of CD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 25ul of the suggested working dilution to label 106 cells or 100ul whole blood.
- Histology Positive Control Tissue
- Spleen, lymph node
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Product Specific References
References for CD4 antibody
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Moore, P.F. et al. (1992) Monoclonal antibodies specific for canine CD4 and CD8 define functional T-lymphocyte subsets and high-density expression of CD4 by canine neutrophils.
Tissue Antigens 40(2): 75-85. -
Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).
Tissue Antigens. 43 (3): 137-54. -
Veenhof, E.Z. et al. (2011) Characterisation of T cell phenotypes, cytokines and transcription factors in the skin of dogs with cutaneous adverse food reactions.
Vet J. 187 (3): 320-4. -
Izci C et al. (2015) Clinical and light microscopic studies of the conjunctival tissues of dogs with bilateral keratoconjunctivitis sicca before and after treatment with topical 2% cyclosporine.
Biotech Histochem. 90 (3): 223-30. -
Ricklin, M.E. et al. (2010) Characterization of canine dendritic cells in healthy, atopic, and non-allergic inflamed skin.
J Clin Immunol. 30 (6): 845-54. -
Wijewardana, V. et al. (2013) Production of canine soluble CD40 ligand to induce maturation of monocyte derived dendritic cells for cancer immunotherapy.
Vet Immunol Immunopathol. 156 (1-2): 121-7. -
Kamiie, J. et al. (2014) Quantitative analysis of CD3ε in a cloned canine lymphoma cell line by selected reaction monitoring assay.
Biosci Biotechnol Biochem. 78 (2): 271-5. -
Heinrich, F. et al. (2015) Immunophenotyping of immune cell populations in the raccoon (Procyon lotor).
Vet Immunol Immunopathol. 168 (3-4): 140-6. -
Yuasa, K. et al. (2007) Injection of a recombinant AAV serotype 2 into canine skeletal muscles evokes strong immune responses against transgene products.
Gene Ther. 14 (17): 1249-60. -
Lin Shiow-Chen et al. (2014) Immune Characterization of Peripheral Blood Mononuclear cells of the Dogs Restored from Innoculation of Canine Transmissible Venereal Tumor Cells.
Tai Vet J. 40 (04): 181-190. -
Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
Parasite Immunol. Jul 27 [Epub ahead of print].
