CD3 antibody | CA17.2A12
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|Mouse anti Dog CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily.
Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalco et al. 2003).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
References for CD3 antibody
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Moore, P.F. and Rossitto, P.V. (1993) Development of monoclonal antibodies to canine T cell receptor complex (TCR/CD3) and their utilisation in the diagnosis of T cell neoplasia.
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McDonough, S. P. and Moore, P. F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis.
Vet Pathol. 37:637-46.
Moore, P.F. et al. (2006) Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.
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Vernau, W and Moore, P. F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.
Vet Immunol Immunopathol. 69:145-64.
Moreno, J. et al (1999) The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy.
Vet Immunol Immunopathol. 71:181-95.
Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-related molecules and cytokines in dogs.
Vet Immunol Immunopathol. 140: 175-80.
Watabe, A. et al. (2011) Alterations of lymphocyte subpopulations in healthy dogs with aging and in dogs with cancer.
Vet Immunol Immunopathol. 142: 189-200.
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Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
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Zentek, J. et al. (2002) Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity.
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Ting-De Ravin, S.S. et al. (2006) Correction of canine X-linked severe combined immunodeficiency by in vivo retroviral gene therapy.
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Miller, J. et al. (2015) Humoral and Cellular Immune Response in Canine Hypothyroidism.
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McGill, J.L. et al. (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.
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do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
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