CD3 antibody | CA17.2A12
Canine T cells labeled with FITC conjugated Mouse anti Canine CD3 antibody, clone CA17.2A12 (MCA1774F) by flow cytometry.
Image caption:
CD11b+CD14−MHCII− cells suppress T cell proliferation. Facs sorted CD11b+CD14−MHCII− cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.
From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Dog CD3 antibody, clone CA17.2A12 (MCA1774GA) used to label canine CD3 expressing cells by immunofluorescence.
Image caption:
Structural organization of canine Peyer’s patches, according to immunofluorescence data.(C) Immunofluorescence staining of PPs. PPs were stained with DAPI in combination with Abs against CD8 and one of the following markers: CD3, CD21, CD4, or FOXP3. Epifluorescence microscopy is shown at original magnification ×10, visualizing specific structures, including the subepithelial dome (SED), T cell areas, and B cell follicles.
From: Miguelena Chamorro B, Hameed SA, Dechelette M, Claude JB, Piney L, Chapat L, Swaminathan G, Poulet H, Longet S, De Luca K, Mundt E, Paul S.
Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 2023 Nov 1;7(11):788-805.
doi: 10.4049/immunohorizons.2300091.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Dog CD3 antibody, clone CA17.2A12 (MCA1774GA) used to label canine CD3 expressing cells by immunofluorescence.
Image caption:
Structural organization of canine Peyer’s patches, according to immunofluorescence data.(C) Immunofluorescence staining of PPs. PPs were stained with DAPI in combination with Abs against CD8 and one of the following markers: CD3, CD21, CD4, or FOXP3. Epifluorescence microscopy is shown at original magnification ×10, visualizing specific structures, including the subepithelial dome (SED), T cell areas, and B cell follicles.
From: Miguelena Chamorro B, Hameed SA, Dechelette M, Claude JB, Piney L, Chapat L, Swaminathan G, Poulet H, Longet S, De Luca K, Mundt E, Paul S.
Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 2023 Nov 1;7(11):788-805.
doi: 10.4049/immunohorizons.2300091.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Fitc conjugated Mouse anti Dog CD3 antibody, clone CA17.2A12 (MCA1774F) used to label lymphocytes in canine peripherla blood samples by flow cytometry.
Image caption:
Gating strategy. Peripheral blood sequential gating strategy for a panel of six antibodies to identify the different cell subpopulations after doublet exclusion. CD45, a pan-leukocyte marker, and CD3, a T-lymphocyte specific marker, were used to define the T-lymphocyte population, with posterior separation of CD4+ and CD8+ cells, CD4+CD8+ double-positive T cells, and subsequent regulatory CD25+FoxP3+ and effector CD25−FoxP3− cells. Red histograms from unstained control samples and colored histograms from single-stained control samples were used to define the sequential gating, along with gray histograms from fluorescence minus one (FMO) controls to gate for rare cells (CD25+FoxP3+).
From: Santos MF, Alexandre-Pires G, Pereira MA, Gomes L, Rodrigues AV, Basso A, Reisinho A, Meireles J, Santos-Gomes GM, Pereira da Fonseca I.
Immunophenotyping of Peripheral Blood, Lymph Node, and Bone Marrow T Lymphocytes During Canine Leishmaniosis and the Impact of Antileishmanial Chemotherapy.
Front Vet Sci. 2020 Jul 15;7:375.
doi: 10.3389/fvets.2020.00375.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Dog CD3
- Product Type
- Monoclonal Antibody
- Clone
- CA17.2A12
- Isotype
- IgG1
- Specificity
- CD3
Antibody Quality and Performance Guarantee
| Mouse anti Dog CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalco et al. 2003). |
- Target Species
- Dog
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/50 |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD3 antibody
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Moore, P.F. and Rossitto, P.V. (1993) Development of monoclonal antibodies to canine T cell receptor complex (TCR/CD3) and their utilisation in the diagnosis of T cell neoplasia.
Vet. Pathol. 30: 457. Abstract 117. -
Vernau, W and Moore, P. F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.
Vet Immunol Immunopathol. 69:145-64. -
Moreno, J. et al (1999) The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy.
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McDonough, S. P. and Moore, P. F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis.
Vet Pathol. 37:637-46. -
Byrne, K. et al (2000) A standardized gating technique for the generation of flow cytometry data for normal canine and normal feline blood lymphocytes.
Vet Immunol Immunopathol. 73:167-82. -
Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
Immunology. 105: 499-508. -
Zentek, J. et al. (2002) Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity.
J Nutr. 132: 1652S-4S. -
Hsiao, Y.W. et al (2004) Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity.
J Immunol. 172: 1508-14.
View The Latest Product References
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Moore, P.F. et al. (2006) Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.
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Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
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Hai, M. et al. (2008) Potential genotoxicity from integration sites in CLAD dogs treated successfully with gammaretroviral vector-mediated gene therapy.
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Watabe, A. et al. (2011) Alterations of lymphocyte subpopulations in healthy dogs with aging and in dogs with cancer.
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Machado, G.F. et al. (2011) Intravascular Lymphomatosis in the Central Nervous System of Dogs: Immunohistochemical Investigation in Two Cases
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Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
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Villaescusa A et al. (2012) Evaluation of peripheral blood lymphocyte subsets in family-owned dogs naturally infected by Ehrlichia canis.
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Aricò, A. et al. (2013) The role of vascular endothelial growth factor and matrix metalloproteinases in canine lymphoma: in vivo and in vitro study.
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Michael, H.T. et al. (2013) Isolation and characterization of canine natural killer cells.
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Duz, A.L. et al. (2014) The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs.
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Aresu, L. et al. (2014) VEGF and MMP-9: biomarkers for canine lymphoma.
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Perosso, J. et al. (2014) Alteration of sFAS and sFAS ligand expression during canine visceral leishmaniosis.
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Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
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Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
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Grøndahl-Rosado C et al. (2015) NCR1+ cells in dogs show phenotypic characteristics of natural killer cells.
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Miller, J. et al. (2015) Humoral and Cellular Immune Response in Canine Hypothyroidism.
J Comp Pathol. 153 (1): 28-37. -
Mie, K. et al. (2016) Change in peripheral blood lymphocyte count in dogs following adoptive immunotherapy using lymphokine-activated T killer cells combined with palliative tumor resection.
Vet Immunol Immunopathol. 177: 58-63. -
Schaut, R.G. et al. (2016) Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant.
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Riondato, F. et al. (2016) Analytical and diagnostic validation of a flow cytometric strategy to quantify blood and marrow infiltration in dogs with large B-cell lymphoma.
Cytometry B Clin Cytom. 90 (6): 525-530. -
Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
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Schaut, R.G. et al. (2016) Regulatory IgDhi B Cells Suppress T Cell Function via IL-10 and PD-L1 during Progressive Visceral Leishmaniasis.
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McGill, J.L. et al. (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.
PLoS One. 11 (2): e0148229. -
Martins, G.C. et al. (2018) Clinical-pathological and immunological biomarkers in dogs with atopic dermatitis.
Vet Immunol Immunopathol. 205: 58-64. -
Schmidli, M.R. et al. (2018) Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease.
BMC Vet Res. 14 (1): 161. -
Akiyama, S. et al. (2019) Th17 cells increase during maturation in peripheral blood of healthy dogs.
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Aguiar-Soares, R.D.O. et al. (2020) Phase I and II Clinical Trial Comparing the LBSap, Leishmune(®), and Leish-Tec(®) Vaccines against Canine Visceral Leishmaniasis.
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Sayag, D. et al. (2020) Proof-of-concept study: Evaluation of plasma and urinary electrolytes as markers of response to L-asparaginase therapy in dogs with high-grade lymphoma.
Vet Clin Pathol. 49 (3): 476-83. -
Enciso, N. et al. (2020) Regenerative potential of allogeneic adipose tissue-derived mesenchymal cells in canine cutaneous wounds.
Acta Vet Scand. 62 (1): 13. -
Marchetti, C. et al. (2020) Profile of gamma-delta (γδ) T lymphocytes in the peripheral blood of crossbreed dogs during stages of life and implication in aging.
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Lee, J. et al. (2021) Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor.
Biomed Res Int. 2021: 6690704. -
Rotolo, A. et al. (2021) Genetic re-direction of canine primary T cells for clinical trial use in pet dogs with spontaneous cancer
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Grudzien, M. et al. (2021) A newly established canine NK-type cell line and its cytotoxic properties.
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Yang, Y. et al. (2021) Canine Multicentric Large B Cell Lymphoma with Increased Mott Cells Diagnosed by Flow Cytometry
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Lee, S.H. et al. (2021) Safety and immunological effects of recombinant canine IL-15 in dogs.
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Knebel, A. et al. (2021) Measurement of canine Th17 cells by flow cytometry.
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Troupel, T. et al. (2022) Generalised idiopathic polymyositis mimicking masticatory myositis in a dog
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do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
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Konno, H. et al. (2022) An experimental challenge model for Leishmania donovani in beagle dogs, showing a similar pattern of parasite burden in the peripheral blood and liver.
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Rotolo, A. et al. (2023) Unedited allogeneic iNKT cells show extended persistence in MHC-mismatched canine recipients.
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Wesolowski, M. et al. (2023) Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniation.
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Yamauchi, A. et al. (2023) Negative Influence of Aging on Differentiation and Proliferation of CD8(+) T-Cells in Dogs.
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Martini, V. et al. (2018) A retrospective study of flow cytometric characterization of suspected extranodal lymphomas in dogs.
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Archer, T.M. et al. (2018) In vivo effects of aspirin and cyclosporine on regulatory T cells and T-cell cytokine production in healthy dogs.
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Lee, G.W. et al. (2021) Case Report: Long-Term Survival of a Dog With Chronic Lymphocytic Leukemia Treated With Chlorambucil, Prednisolone, and Imatinib.
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Sainz, Á. et al. (2021) Effect of chemically modified tetracycline-8 (CMT-8) on hematology, blood chemistry, cytokines and peripheral blood lymphocyte subsets of healthy dogs.
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Hughes, K. et al. (2024) Canine T zone lymphoma is a tumor of mature, previously activated αβ T cells
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Anthonyraj, S. et al. (2024) Chicory root powder included as a prebiotic in different cereal-based diets for dogs: Influences on gut health, metabolic and immunological status
Bioactive Carbohydrates and Dietary Fibre. : 100414. [Pre-proof] -
Sheng, R. et al. (2023) Prognostic significance of CD25 expression in dogs with a noninvasive diagnosis of B-cell lymphoma treated with CHOP chemotherapy.
Vet Comp Oncol. 21 (1): 28-35. -
Miguelena Chamorro, B. et al. (2023) Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
Immunohorizons. 7 (11): 788-805. -
Santos, M.F. et al. (2020) Immunophenotyping of Peripheral Blood, Lymph Node, and Bone Marrow T Lymphocytes During Canine Leishmaniosis and the Impact of Antileishmanial Chemotherapy.
Front Vet Sci. 7: 375. -
Karai, E. et al. (2020) Celecoxib Prevents Doxorubicin-Induced Multidrug Resistance in Canine and Mouse Lymphoma Cell Lines.
Cancers (Basel). 12 (5): 1117.
- RRID
- AB_1125250
- UniProt
- P27597
- Entrez Gene
- CD3E
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
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