WC4 antibody | CC55

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Mouse anti Bovine WC4

Product Type
Monoclonal Antibody
Clone
CC55
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1648G C F IP 0.25 mg
Mouse anti Bovine WC4 antibody, clone CC55 recognizes the bovine WC4 cell surface antigen, a ~90kDa molecule expressed by a subpopulation of B cells in peripheral blood and lymphoid tissues (Howard et al. 1993). It is suggested that WC4 like SWC7 in pigs is the bovine orthologue of human CD19 (Naessens and Howard 1991, Ikebuchi et al. 2013).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of WC4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for WC4 antibody

  1. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
    Vet Immunol Immunopathol. 27 (1-3): 21-7.
  2. Denham, S. et al. (1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.
    Vet Immunol Immunopathol. 43 (1-3): 259-67.
  3. Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
    Immunol Today. 18 (8): 365-8.
  4. Boersma, W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
    Vet Immunol Immunopathol. 80 (1-2): 63-78.
  5. Andersen, J.K. <et al. (1999) Systematic characterization of porcine ileal Peyer's patch, I. apoptosis-sensitive immature B cells are the predominant cell type.
    Immunology. 98: 612-21.
  6. Szymańska-Czerwińska, M. et al. (2009) Effect of tylosin and prebiotics on the level of cytokines and lymphocyte immunophenotyping parameters in calves
    Central European Journal of Immunology. 34: 1-6.
  7. Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.
    Vet Res. 44: 59.
  8. Ikebuchi, R. et al. (2014) Differences in cellular function and viral protein expression between IgMhigh and IgMlow B-cells in bovine leukemia virus-infected cattle.
    J Gen Virol. 95: 1832-42.
  9. Nishimori, A. et al. (2016) Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection.
    J Vet Med Sci. 78 (5): 791-6.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.