WC4 antibody | CC55
Mouse anti Bovine WC4
- Product Type
- Monoclonal Antibody
- Clone
- CC55
- Isotype
- IgG1
- Specificity
- WC4
Antibody Quality and Performance Guarantee
| Mouse anti Bovine WC4 antibody, clone CC55 recognizes the bovine WC4 cell surface antigen, a ~90kDa molecule expressed by a subpopulation of B cells in peripheral blood and lymphoid tissues (Howard et al. 1993). It is suggested that WC4, like SWC7 in pigs, is the bovine orthologue of human CD19 (Naessens and Howard 1991, Ikebuchi et al. 2013). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for WC4 antibody
-
Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle.
Vet Immunol Immunopathol. 27 (1-3): 21-7. -
Denham, S. et al. (1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.
Vet Immunol Immunopathol. 43 (1-3): 259-67. -
Naessens, J. et al. (1997) Nomenclature and characterization of leukocyte differentiation antigens in ruminants.
Immunol Today. 18 (8): 365-8. -
Andersen, J.K. <et al. (1999) Systematic characterization of porcine ileal Peyer's patch, I. apoptosis-sensitive immature B cells are the predominant cell type.
Immunology. 98: 612-21. -
Boersma, W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
Vet Immunol Immunopathol. 80 (1-2): 63-78. -
Szymańska-Czerwińska, M. et al. (2009) Effect of tylosin and prebiotics on the level of cytokines and lymphocyte immunophenotyping parameters in calves
Central European Journal of Immunology. 34: 1-6. -
Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.
Vet Res. 44: 59. -
Ikebuchi, R. et al. (2014) Differences in cellular function and viral protein expression between IgMhigh and IgMlow B-cells in bovine leukemia virus-infected cattle.
J Gen Virol. 95: 1832-42.
View The Latest Product References
-
Nishimori, A. et al. (2016) Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection.
J Vet Med Sci. 78 (5): 791-6. -
Maciag, S.S. et al. (2022) On the influence of the source of porcine colostrum in the development of early immune ontogeny in piglets.
Sci Rep. 12 (1): 15630. -
Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
Virol J. 20 (1): 181. -
Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
Vet Immunol Immunopathol. 254: 110520. -
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- CD19
- SWC7
MCA1648GA
150230
165882
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