Superoxide Dismutase antibody | 2F5

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Mouse anti Superoxide Dismutase (Cu-Zn)

Product Type
Monoclonal Antibody
Clone
2F5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2595 C E P 0.1 mg
Mouse anti Bovine superoxide dismutase (Cu/Zn) antibody, clone 2F5 recognizes bovine superoxide dismutase (Cu/Zn), also known by it's gene name SOD1. Bovine superoxide dismutase (Cu/Zn) is a 152 amino acid ~15kDa cytoplasmic protein involved in the removal of free radicals from the cytosol. Superoxide dismutases are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. There are three major families of superoxide dismutase, depending on the metal cofactor: Cu-Zn (which binds both copper and zinc), Fe and Mn types (which bind either iron or manganese), and finally the Ni type, which binds nickel.

Mouse anti Bovine superoxide dismutase (Cu/Zn) antibody, clone 2F5 does not cross react with immunoglobulins but does react weakly with Mn SOD.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
0.1%Bovine Serum Albumin
Immunogen
Superoxide dismutase enzyme prepared from bovine erythrocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised mice were fused with cells of the mouse Sp-2/0-Ag14 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P00442 Related reagents
Entrez Gene
SOD1 Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0000209 protein polyubiquitination
GO:0001541 ovarian follicle development
GO:0001819 positive regulation of cytokine production
GO:0001895 retina homeostasis
GO:0002262 myeloid cell homeostasis
GO:0051087 chaperone binding
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0004784 superoxide dismutase activity
GO:0004842 ubiquitin-protein ligase activity
GO:0005507 copper ion binding
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0055114 oxidation-reduction process
GO:0006749 glutathione metabolic process
GO:0006302 double-strand break repair
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006879 cellular iron ion homeostasis
GO:0007283 spermatogenesis
GO:0007566 embryo implantation
GO:0007569 cell aging
GO:0007605 sensory perception of sound
GO:0007626 locomotory behavior
GO:0008217 regulation of blood pressure
GO:0008270 zinc ion binding
GO:0042493 response to drug
GO:0009408 response to heat
GO:0019430 removal of superoxide radicals
GO:0030346 protein phosphatase 2B binding
GO:0031012 extracellular matrix
GO:0031410 cytoplasmic vesicle
GO:0032287 peripheral nervous system myelin maintenance
GO:0032839 dendrite cytoplasm
GO:0040014 regulation of multicellular organism growth
GO:0042542 response to hydrogen peroxide
GO:0043025 neuronal cell body
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0043234 protein complex
GO:0043524 negative regulation of neuron apoptosis
GO:0044419 interspecies interaction between organisms
GO:0045471 response to ethanol
GO:0045541 negative regulation of cholesterol biosynthetic process
GO:0046716 muscle cell homeostasis
GO:0048678 response to axon injury
GO:0050665 hydrogen peroxide biosynthetic process
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
GO:0051881 regulation of mitochondrial membrane potential
GO:0060047 heart contraction
GO:0060052 neurofilament cytoskeleton organization
GO:0060087 relaxation of vascular smooth muscle
GO:0060088 auditory receptor cell stereocilium organization
Regulatory
For research purposes only

Applications of Superoxide Dismutase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
This product does not require pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.
Histology Positive Control Tissue
Human spleen and lymph node.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P

Product Specific References

References for Superoxide Dismutase antibody

  1. Paździor-Czapula, K. et al. (2014) Immunohistochemical evaluation of superoxide dismutase (Cu/Zn SOD) concentrations in erythrocytes of dairy cattle and farm-raised deer by a computer-assisted analysis of microscopic images.
    Pol J Vet Sci. 17: 275-9.