PI-3 Kinase p85 Subunit Beta antibody | T15
Mouse anti PI-3 Kinase p85 Subunit beta antibody, clone T15 recognizes the p85 subunit of bovine PI-3 kinase.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Balculovirus expressed (Recombinant) p85 beta subunit of bovine PI-3 kinase.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the NS1 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred
This product should be stored undiluted
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P23726 Related reagents
- O00459 Related reagents
- O08908 Related reagents
- Q63788 Related reagents
- Entrez Gene
- PIK3R2 Related reagents
- Pik3r2 Related reagents
- PIK3R2 Related reagents
- Pik3r2 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005096 GTPase activator activity
- GO:0005942 phosphatidylinositol 3-kinase complex
- GO:0007264 small GTPase mediated signal transduction
- GO:0008286 insulin receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0019987 negative regulation of anti-apoptosis
- GO:0030168 platelet activation
- GO:0031295 T cell costimulation
- GO:0035014 phosphatidylinositol 3-kinase regulator activity
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050852 T cell receptor signaling pathway
- GO:0050900 leukocyte migration
- GO:0051056 regulation of small GTPase mediated signal transduction
- GO:0007165 signal transduction
- Regulatory
- For research purposes only
Applications of PI-3 Kinase p85 Subunit Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunoprecipitation | 10 ug/ml | ||
| Western Blotting | 1/500 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for PI-3 Kinase p85 Subunit Beta antibody
-
Brehme,M. et al (2009) Charting the molecular network of the drug target Bcr-Abl.
Proc Natl Acad Sci U S A,. 106: 7414-9 -
Hale, B.G. et al. (2008) Binding of influenza A virus NS1 protein to the inter-SH2 domain of p85 suggests a novel mechanism for phosphoinositide 3-kinase activation.
J Biol Chem. 283: 1372-80. -
Ehrhardt, C. et al. (2007) Influenza A virus NS1 protein activates the PI3K/Akt pathway to mediate antiapoptotic signaling responses.
J Virol. 81: 3058-67. -
Massone, C. et al. (2011) Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: a scenario dependent on CD8+ and/or CD20+ cells.
Br J Dermatol. 165: 321-8. -
Yokoyama, K. et al. (2011) NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons.
EMBO J. 30: 4739-54. -
Ylösmäki, L. et al. (2015) Reorganization of the host cell Crk(L)-PI3 kinase signaling complex by the influenza A virus NS1 protein.
Virology. 484: 146-52.
