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PI-3 Kinase p85 Subunit Beta antibody | T15

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Anti PI-3 Kinase p85 Subunit Beta Antibody, clone T15 gallery image 1
Western Blotting of HEK293 and HepG2 cell lysates with Mouse anti Bovine PI-3 kinase p85 subunit beta (MCA1170), recombinant p85β is included as a positive control

Mouse anti PI-3 Kinase p85 Subunit Beta

Product Type
Monoclonal Antibody
Clone
T15
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1170G
Datasheet
C IP WB
SDS
0.1 mg loader

Mouse anti PI-3 Kinase p85 Subunit beta antibody, clone T15 recognizes the p85 subunit of bovine PI-3 kinase.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Balculovirus expressed (Recombinant) p85 beta subunit of bovine PI-3 kinase.
Approx. Protein Concentrations
1.0 mg/ml
Fusion Partners
Spleen cells from immunized mice were fused with cells of the NS1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred

This product should be stored undiluted

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P23726
O00459
O08908
Q63788
Entrez Gene
PIK3R2
Pik3r2
PIK3R2
Pik3r2
GO Terms
GO:0005515 protein binding
GO:0005096 GTPase activator activity
GO:0005942 phosphatidylinositol 3-kinase complex
GO:0007264 small GTPase mediated signal transduction
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0048015 phosphatidylinositol-mediated signaling
GO:0019987 negative regulation of anti-apoptosis
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0035014 phosphatidylinositol 3-kinase regulator activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
GO:0051056 regulation of small GTPase mediated signal transduction
GO:0007165 signal transduction
Regulatory
For research purposes only

Applications of PI-3 Kinase p85 Subunit Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunoprecipitation 10 ug/ml
Western Blotting 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunoprecipitation

Western Blotting

Product Specific References

References for PI-3 Kinase p85 Subunit Beta antibody

  1. Brehme,M. et al (2009) Charting the molecular network of the drug target Bcr-Abl.
    Proc Natl Acad Sci U S A,. 106: 7414-9
  2. Hale, B.G. et al. (2008) Binding of influenza A virus NS1 protein to the inter-SH2 domain of p85 suggests a novel mechanism for phosphoinositide 3-kinase activation.
    J Biol Chem. 283: 1372-80.
  3. Ehrhardt, C. et al. (2007) Influenza A virus NS1 protein activates the PI3K/Akt pathway to mediate antiapoptotic signaling responses.
    J Virol. 81: 3058-67.
  4. Massone, C. et al. (2011) Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: a scenario dependent on CD8+ and/or CD20+ cells.
    Br J Dermatol. 165: 321-8.
  5. Yokoyama, K. et al. (2011) NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons.
    EMBO J. 30: 4739-54.
  6. Ylösmäki, L. et al. (2015) Reorganization of the host cell Crk(L)-PI3 kinase signaling complex by the influenza A virus NS1 protein.
    Virology. 484: 146-52.

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