Mouse anti PI-3 Kinase p85 Subunit beta antibody, clone T15 recognizes the p85 subunit of bovine PI-3 kinase.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Balculovirus expressed (Recombinant) p85 beta subunit of bovine PI-3 kinase.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the NS1 mouse myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
GTPase activator activity
phosphatidylinositol 3-kinase complex
small GTPase mediated signal transduction
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
negative regulation of anti-apoptosis
T cell costimulation
phosphatidylinositol 3-kinase regulator activity
nerve growth factor receptor signaling pathway
T cell receptor signaling pathway
regulation of small GTPase mediated signal transduction
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PI-3 Kinase p85 Subunit Beta antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Application Based External Images
Product Specific References
References for PI-3 Kinase p85 Subunit Beta antibody
Brehme,M. et al (2009) Charting the molecular network of the drug target Bcr-Abl.
Proc Natl Acad Sci U S A,. 106: 7414-9
Hale, B.G. et al. (2008) Binding of influenza A virus NS1 protein to the inter-SH2 domain of p85 suggests a novel mechanism for phosphoinositide 3-kinase activation.
J Biol Chem. 283: 1372-80.
Ehrhardt, C. et al. (2007) Influenza A virus NS1 protein activates the PI3K/Akt pathway to mediate antiapoptotic signaling responses.
J Virol. 81: 3058-67.
Massone, C. et al. (2011) Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: a scenario dependent on CD8+ and/or CD20+ cells.
Br J Dermatol. 165: 321-8.
Yokoyama, K. et al. (2011) NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons.
EMBO J. 30: 4739-54.
Ylösmäki, L. et al. (2015) Reorganization of the host cell Crk(L)-PI3 kinase signaling complex by the influenza A virus NS1 protein.
Virology. 484: 146-52.
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