PI-3 Kinase p85 Subunit Beta antibody | T15
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WB ResetMouse anti PI-3 Kinase p85 Subunit Beta
- Product Type
- Monoclonal Antibody
- Clone
- T15
- Isotype
- IgG1
- Specificity
- PI-3 Kinase p85 Subunit Beta
Antibody Quality and Performance Guarantee
| Mouse anti PI-3 Kinase p85 Subunit beta antibody, clone T15 recognizes the p85 subunit of bovine PI-3 kinase. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Balculovirus expressed (Recombinant) p85 beta subunit of bovine PI-3 kinase.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the NS1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Immunoprecipitation | |
10 ug/ml | |
| Western Blotting | |
1/500 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for PI-3 Kinase p85 Subunit Beta antibody
-
Brehme,M. et al (2009) Charting the molecular network of the drug target Bcr-Abl.
Proc Natl Acad Sci U S A,. 106: 7414-9 -
Hale, B.G. et al. (2008) Binding of influenza A virus NS1 protein to the inter-SH2 domain of p85 suggests a novel mechanism for phosphoinositide 3-kinase activation.
J Biol Chem. 283: 1372-80. -
Ehrhardt, C. et al. (2007) Influenza A virus NS1 protein activates the PI3K/Akt pathway to mediate antiapoptotic signaling responses.
J Virol. 81: 3058-67. -
Massone, C. et al. (2011) Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: a scenario dependent on CD8+ and/or CD20+ cells.
Br J Dermatol. 165: 321-8. -
Yokoyama, K. et al. (2011) NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons.
EMBO J. 30: 4739-54. -
Ylösmäki, L. et al. (2015) Reorganization of the host cell Crk(L)-PI3 kinase signaling complex by the influenza A virus NS1 protein.
Virology. 484: 146-52.
- RRID
- AB_2163464
- UniProt
- P23726
- O00459
- O08908
- Q63788
- Entrez Gene
- PIK3R2
- Pik3r2
- PIK3R2
- Pik3r2
- GO Terms
- GO:0005515 protein binding
- GO:0005096 GTPase activator activity
- GO:0005942 phosphatidylinositol 3-kinase complex
- GO:0007264 small GTPase mediated signal transduction
- GO:0008286 insulin receptor signaling pathway
- GO:0008543 fibroblast growth factor receptor signaling pathway
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0019987 negative regulation of anti-apoptosis
- GO:0030168 platelet activation
- View More GO Terms
- GO:0031295 T cell costimulation
- GO:0035014 phosphatidylinositol 3-kinase regulator activity
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050852 T cell receptor signaling pathway
- GO:0050900 leukocyte migration
- GO:0051056 regulation of small GTPase mediated signal transduction
- GO:0007165 signal transduction
MCA1170G
151272
1707
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