Myelin Proteolipid Protein antibody | plpc1

100% Secure

Mouse anti Myelin Proteolipid Protein

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Mouse anti myelin proteolipid protein antibody, clone plpc1 recognizes myelin proteolipid protein (PLP) in many mammalian species (Stoffel et al. 1985). Clone plpc1 also recognizes the alternative PLP splice variant lacking part of the cytoplasmic domain (amino acids 117-151), known as DM20 (Simons et al. 1987) .

PLP encodes the major protein components of compact CNS myelin and mutations in the PLP gene can lead to severe dysmyelinating disease (Hudson et al. 1989).
Mouse anti myelin proteolipid protein, clone plpc1 has proved a useful immunohistochemical tool for the study of central nervous system injury in patients with multiple sclerosis (Seewan et al. 2011, Huizinga et al. 2011)

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Tenerife lizard (Gallotia galloti)
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Synthetic peptide GRGTKF corresponding to C terminal region of myelin proteolipid protein.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0016021 integral to membrane
For research purposes only

Applications of Myelin Proteolipid Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader

Application Based External Images


Immunohistology - Frozen

Immunohistology - Paraffin

Product Specific References

References for Myelin Proteolipid Protein antibody

  1. Pomeroy, I.M. et al. (2005) Demyelinated neocortical lesions in marmoset autoimmune encephalomyelitis mimic those in multiple sclerosis.
    Brain. 128: 2713-21.
  2. Grade, S. et al. (2010) Functional identification of neural stem cell-derived oligodendrocytes by means of calcium transients elicited by thrombin.
    Rejuvenation Res.13: 27-37.
  3. Moharregh-Khiabani, D. et al. (2010) Effects of fumaric acids on cuprizone induced central nervous system de- and remyelination in the mouse.
    PLoS One. 5:e11769.
  4. Bramow, S. et al. (2010) Demyelination versus remyelination in progressive multiple sclerosis.
    Brain.133: 2983-98.
  5. Kooij, G. et al. (2010) Adenosine triphosphate-binding cassette transporters mediate chemokine (C-C motif) ligand 2 secretion from reactive astrocytes: relevance to multiple sclerosis pathogenesis.
    Brain. 134: 555-70.
  6. Jaśkiewicz, E. et al. (2005) Expression of recombinant forms of human 21.5 kDa myelin basic protein and proteolipid protein in CHO cells.
    Acta. Biochim. Pol. 52: 863-6.
  7. Coulpier, F. et al. (2010) CNS/PNS boundary transgression by central glia in the absence of Schwann cells or Krox20/Egr2 function.
    J Neurosci. 30: 5958-67.
  8. Gilmore, C.P. et al. (2006) Spinal cord gray matter demyelination in multiple sclerosis-a novel pattern of residual plaque morphology.
    Brain Pathol. 16: 202-8.
  9. Boon, L. et al. (2001) Prevention of experimental autoimmune encephalomyelitis in the common marmoset (Callithrix jacchus) using a chimeric antagonist monoclonal antibody against human CD40 is associated with altered B cell responses.
    J Immunol. 167: 2942-9.
  10. Roemer, S.F. et al. (2007) Pattern-specific loss of aquaporin-4 immunoreactivity distinguishes neuromyelitis optica from multiple sclerosis.
    Brain. 130: 1194-205.
  11. Haider, L. et al. (2011) Oxidative damage in multiple sclerosis lesions.
    Brain. 134: 1914-24.
  12. Baeten, K. et al. (2011) Tracking of myelin-reactive T cells in experimental autoimmune encephalomyelitis (EAE) animals using small particles of iron oxide and MRI.
    NMR Biomed. 23: 601-9.
  13. Geurts, J.J. et al. (2007) Extensive hippocampal demyelination in multiple sclerosis.
    J Neuropathol Exp Neurol. 66: 819-27.
  14. Kooi, E.J. et al. (2011) Cholinergic imbalance in the multiple sclerosis hippocampus.
    Acta Neuropathol. 122: 313-22.
  15. van Horssen, J. et al. (2006) NAD(P)H:quinone oxidoreductase 1 expression in multiple sclerosis lesions.
    Free Radic Biol Med. 41: 311-7.
  16. van Horssen, J. et al. (2010) Nrf2 and DJ1 are consistently upregulated in inflammatory multiple sclerosis lesions.
    Free Radic Biol Med. 49: 1283-9.
  17. Seewann, A. et al. (2012) Postmortem verification of MS cortical lesion detection with 3D DIR.
    Neurology. 78: 302-8.
  18. Popescu, B.F. et al. (2010) Absence of cortical demyelination in neuromyelitis optica.
    Neurology. 75: 2103-9.
  19. Kooi, E.J. et al. (2012) Heterogeneity of cortical lesions in multiple sclerosis: clinical and pathologic implications.
    Neurology. 79 (13): 1369-76.
  20. Skripuletz, T. et al. (2013) Astrocytes regulate myelin clearance through recruitment of microglia during cuprizone-induced demyelination.
    Brain. 136 (Pt 1): 147-67.
  21. Bagnato, F. et al. (2011) Tracking iron in multiple sclerosis: a combined imaging and histopathological study at 7 Tesla.
    Brain. 134: 3602-15.
  22. Bramow, S. et al. (2010) Demyelination versus remyelination in progressive multiple sclerosis.
    Brain. 133: 2983-98.
  23. Dunham, J. et al. (2017) Severe oxidative stress in an acute inflammatory demyelinating model in the rhesus monkey.
    PLoS One. 12 (11): e0188013.
  24. Janssen, K. et al. (2016) Absence of CCL2 and CCL3 Ameliorates Central Nervous System Grey Matter But Not White Matter Demyelination in the Presence of an Intact Blood-Brain Barrier.
    Mol Neurobiol. 53 (3): 1551-64.
  25. Betzer, C. et al. (2015) Identification of Synaptosomal Proteins Binding to Monomeric and Oligomeric α-Synuclein.
    PLoS One. 10: e0116473.
  26. Jatana, M. et al. (2006) Combination of systemic hypothermia and N-acetylcysteine attenuates hypoxic-ischemic brain injury in neonatal rats.
    Pediatr Res. 59 (5): 684-9.
  27. DeBruin, L.S. et al. (2005) Developmental partitioning of myelin basic protein into membrane microdomains.
    J Neurosci Res. 80 (2): 211-25.
  28. Santos, E. et al. (2006) Peculiar and typical oligodendrocytes are involved in an uneven myelination pattern during the ontogeny of the lizard visual pathway.
    J Neurobiol. 66 (10): 1115-24.
  29. van Horssen, J. et al. (2012) Clusters of activated microglia in normal-appearing white matter show signs of innate immune activation.
    J Neuroinflammation. 9: 156.
  30. Hinson, S.R. et al. (2012) Molecular outcomes of neuromyelitis optica (NMO)-IgG binding to aquaporin-4 in astrocytes.
    Proc Natl Acad Sci U S A. 109: 1245-50.
  31. Maccarrone, G. et al. (2016) MALDI imaging mass spectrometry analysis-A new approach for protein mapping in multiple sclerosis brain lesions.
    J Chromatogr B Analyt Technol Biomed Life Sci. Jul 1. pii: S1570-0232(16)30452-4. [Epub ahead of print]
  32. Chuang, T.Y. et al. (2016) LRP1 expression in microglia is protective during CNS autoimmunity.
    Acta Neuropathol Commun. 4 (1): 68.
  33. Cerina, M. et al. (2016) The quality of cortical network function recovery depends on localization and degree of axonal demyelination.
    Brain Behav Immun. Aug 25. pii: S0889-1591(16)30393-2. [Epub ahead of print]
  34. Magalon, K. et al. (2016) Olesoxime favors oligodendrocyte differentiation through a functional interplay between mitochondria and microtubules.
    Neuropharmacology. 111: 293-303.
  35. Russi, A.E. et al. (2016) Meningeal mast cell-T cell crosstalk regulates T cell encephalitogenicity.
    J Autoimmun. 73: 100-10.
  36. Jonkman, L.E. et al. (2016) Ultra-high field MTR and qR2* differentiates subpial cortical lesions from normal-appearing gray matter in multiple sclerosis.
    Mult Scler. 22 (10): 1306-14.
  37. van Horssen, J. et al. (2016) Human endogenous retrovirus W in brain lesions: Rationale for targeted therapy in multiple sclerosis.
    Mult Scler Relat Disord. 8: 11-8.
  38. Shakhbazau, A. et al. (2016) Demyelination induces transport of ribosome-containing vesicles from glia to axons: evidence from animal models and MS patient brains.
    Mol Biol Rep. 43 (6): 495-507.
  39. Kilsdonk, I.D. et al. (2016) Increased cortical grey matter lesion detection in multiple sclerosis with 7 T MRI: a post-mortem verification study.
    Brain. 139 (Pt 5): 1472-81.
  40. Dooves, S. et al. (2016) Astrocytes are central in the pathomechanisms of vanishing white matter.
    J Clin Invest. 126 (4): 1512-24.
  41. Clarner, T. et al. (2015) CXCL10 triggers early microglial activation in the cuprizone model.
    J Immunol. 194 (7): 3400-13.
  42. Nakajima, M. et al. (2016) Auraptene induces oligodendrocyte lineage precursor cells in a cuprizone-induced animal model of demyelination.
    Brain Res. 1639: 28-37.
  43. Skripuletz, T. et al. (2015) Pivotal role of choline metabolites in remyelination.
    Brain. 138 (Pt 2): 398-413.
  44. Klok, M.D. et al. (2015) Interferon-α and the calcifying microangiopathy in Aicardi-Goutières syndrome.
    Ann Clin Transl Neurol. 2 (7): 774-9.
  45. Popescu, V. et al. (2015) What drives MRI-measured cortical atrophy in multiple sclerosis?
    Mult Scler. 21 (10): 1280-90.
  46. Barateiro, A. et al. (2016) S100B as a Potential Biomarker and Therapeutic Target in Multiple Sclerosis.
    Mol Neurobiol. 53 (6): 3976-91.
  47. Alme, M.N. et al. (2015) Fingolimod does not enhance cerebellar remyelination in the cuprizone model.
    J Neuroimmunol. 285: 180-6.
  48. Fjær, S. et al. (2015) Magnetization transfer ratio does not correlate to myelin content in the brain in the MOG-EAE mouse model.
    Neurochem Int. 83-84: 28-40.
  49. Ni, W. et al. (2015) Deferoxamine reduces intracerebral hemorrhage-induced white matter damage in aged rats.
    Exp Neurol. 272: 128-34.
  50. Tobin, W.O. et al. (2016) Clinical-radiological-pathological spectrum of central nervous system-idiopathic inflammatory demyelinating disease in the elderly.
    Mult Scler. Oct 19. pii: 1352458516675748. [Epub ahead of print]
  51. Michailidou, I. et al. (2016) Complement C3 on microglial clusters in multiple sclerosis occur in chronic but not acute disease: Implication for disease pathogenesis.
    Glia. Oct 25. [Epub ahead of print]
  52. Cerina, M. et al. (2017) The quality of cortical network function recovery depends on localization and degree of axonal demyelination.
    Brain Behav Immun. 59: 103-17.
  53. Zimmermann, J. et al. (2017) IL-17A Promotes Granulocyte Infiltration, Myelin Loss, Microglia Activation, and Behavioral Deficits During Cuprizone-Induced Demyelination.
    Mol Neurobiol. Jan 13. [Epub ahead of print]
  54. Zendedel, A. et al. (2016) Regulatory effect of triiodothyronine on brain myelination and astrogliosis after cuprizone-induced demyelination in mice.
    Metab Brain Dis. 31 (2): 425-33.
  55. Bihler, K. et al. (2017) Formyl Peptide Receptor 1-Mediated Glial Cell Activation in a Mouse Model of Cuprizone-Induced Demyelination.
    J Mol Neurosci. May 2 [Epub ahead of print]
  56. Bagnato, F. et al. (2018) Untangling the R2* contrast in multiple sclerosis: A combined MRI-histology study at 7.0 Tesla.
    PLoS One. 13 (3): e0193839.
  57. McKavanagh, R. et al. (2019) Relating diffusion tensor imaging measurements to microstructural quantities in the cerebral cortex in multiple sclerosis.
    Hum Brain Mapp. 40 (15): 4417-31.
  58. Kamermans, A. et al. (2019) Setmelanotide, a Novel, Selective Melanocortin Receptor-4 Agonist Exerts Anti-inflammatory Actions in Astrocytes and Promotes an Anti-inflammatory Macrophage Phenotype.
    Front Immunol. 10: 2312.
  59. Bulk, M. et al. (2020) Pathological characterization of T2*-weighted MRI contrast in the striatum of Huntington’s disease patients
    NeuroImage: Clinical. 28: 102498.
  60. Rohr, S.O. et al. (2020) Aquaporin-4 Expression during Toxic and Autoimmune Demyelination.
    Cells. 9 (10) Sep 28 [Epub ahead of print].
  61. Böttcher, C. et al. (2020) Single-cell mass cytometry reveals complex myeloid cell composition in active lesions of progressive multiple sclerosis.
    Acta Neuropathol Commun. 8 (1): 136.
  62. van Wageningen, T.A. et al. (2019) Regulation of microglial TMEM119 and P2RY12 immunoreactivity in multiple sclerosis white and grey matter lesions is dependent on their inflammatory environment.
    Acta Neuropathol Commun. 7 (1): 206.
  63. Esser, S. et al. (2018) Toll-Like Receptor 2-Mediated Glial Cell Activation in a Mouse Model of Cuprizone-Induced Demyelination.
    Mol Neurobiol. 55 (8): 6237-49.
  64. Ahmad, I. et al. (2021) A higher proportion of ermin-immunopositive oligodendrocytes in areas of remyelination.
    PLoS One. 16 (8): e0256155.
  65. de Jong C. et al. (2018) Galectin-4, a Negative Regulator of Oligodendrocyte Differentiation, Is Persistently Present in Axons and Microglia/Macrophages in Multiple Sclerosis Lesions.
    J Neuropathol Exp Neurol. 77 (11): 1024-38.
  66. Gudi, V. et al. (2021) Regenerative Effects of CDP-Choline: A Dose-Dependent Study in the Toxic Cuprizone Model of De- and Remyelination
    Pharmaceuticals. 14 (11): 1156.
  67. Guo, Y. et al. (2021) Spectrum of sublytic astrocytopathy in neuromyelitis optica.
    Brain. Oct 30; awab394 [Epub ahead of print].
  68. Yates, R.L. et al. (2021) The influence of HLA-DRB1*15 on the relationship between microglia and neurons in multiple sclerosis normal appearing cortical grey matter.
    Brain Pathol. 2021: e13041.
  69. Tham, M. et al. (2021) Iron Heterogeneity in Early Active Multiple Sclerosis Lesions.
    Ann Neurol. 89 (3): 498-510.
  70. Helman, G. et al. (2021) Cerebral Microangiopathy in Leukoencephalopathy With Cerebral Calcifications and Cysts: A Pathological Description.
    J Child Neurol. 36 (2): 133-40.
  71. Kolb, H. et al. (2021) 7T MRI Differentiates Remyelinated from Demyelinated Multiple Sclerosis Lesions.
    Ann Neurol. 90 (4): 612-26.
  72. Miedema, A. et al. (2021) Brain macrophages acquire distinct transcriptomes prior to demyelination in multiple sclerosis
    bioRxiv. Oct 28 [Epub ahead of print].
  73. Schultz, V. et al. (2021) Zika Virus Infection Leads to Demyelination and Axonal Injury in Mature CNS Cultures.
    Viruses. 13(1):91.
  74. Vanoevelen, J.M. et al. (2021) DTYMK is essential for genome integrity and neuronal survival.
    Acta Neuropathol. Dec 17 [Epub ahead of print].

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra