MHC Class I Monomorphic antibody | IL-A88
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F ResetMouse anti Bovine MHC Class I Monomorphic
- Product Type
- Monoclonal Antibody
- Clone
- IL-A88
- Isotype
- IgG2a
- Specificity
- MHC Class I Monomorphic
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| Mouse anti Bovine MHC Class I Monomorphic antibody, clone IL-A88 recognizes a monomorphic determinant within the heavy chain of bovine MHC Class I. Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This antibody immunoprecipitates a band of approximately 45 kDa under reducing conditions. |
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for MHC Class I Monomorphic antibody
-
Ambagala, A.P. et al. (2000) An early pseudorabies virus protein down-regulates porcine MHC class I expression by inhibition of transporter associated with antigen processing (TAP).
J Immunol. 164 (1): 93-9. -
Daubenberger CA et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parva.
Infect Immun. 67 (5): 2241-9. -
Araibi, E.H. et al. (2004) Downregulation of major histocompatibility complex class I in bovine papillomas.
J Gen Virol. 85 (Pt 10): 2809-14. -
Ashrafi, G.H. et al. (2002) Down-regulation of MHC class I by bovine papillomavirus E5 oncoproteins.
Oncogene. 21: 248-59. -
Suzuki, T. et al. (2003) Evaluation of the delta subunit of bovine adaptor protein complex 3 as a receptor for bovine leukaemia virus.
J Gen Virol. 84 (Pt 5): 1309-16. -
Stephens SA & Howard CJ (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
Parasitology. 124 (Pt 5): 485-93. -
Toye, P.G. et al. (1990) Transfection into mouse L cells of genes encoding two serologically and functionally distinct bovine class I MHC molecules from a MHC-homozygous animal: evidence for a second class I locus in cattle.
Immunology. 70: 20-6. -
Marchetti, B. et al. (2002) The bovine papillomavirus oncoprotein E5 retains MHC class I molecules in the Golgi apparatus and prevents their transport to the cell surface.
Oncogene. 21:7808-16
View The Latest Product References
-
Bainbridge, DR. et al. (2001) Increased expression of major histocompatibility complex (MHC) class I transplantation antigens in bovine trophoblast cells before fusion with maternal cells.
Reproduction.122: 907-13. -
Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
Immunology. 108: 55-61. -
Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
PLoS One. 11 (9): e0162571. -
Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
Comp Immunol Microbiol Infect Dis. 68: 101388. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365.
- UniProt
- Q30289
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