MHC Class I Monomorphic antibody | IL-A88
Mouse anti Bovine MHC Class I Monomorphic antibody, clone IL-A88 recognizes a monomorphic determinant within the heavy chain of bovine MHC Class I.
Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This antibody immunoprecipitates a band of approximately 45 kDa under reducing conditions.
Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This antibody immunoprecipitates a band of approximately 45 kDa under reducing conditions.
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q30289 Related reagents
- Regulatory
- For research purposes only
Applications of MHC Class I Monomorphic antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/25 | 1/200 | |
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F |
Product Specific References
References for MHC Class I Monomorphic antibody
-
Ambagala, A.P. et al. (2000) An early pseudorabies virus protein down-regulates porcine MHC class I expression by inhibition of transporter associated with antigen processing (TAP).
J Immunol. 164 (1): 93-9. -
Daubenberger CA et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parva.
Infect Immun. 67 (5): 2241-9. -
Araibi, E.H. et al. (2004) Downregulation of major histocompatibility complex class I in bovine papillomas.
J Gen Virol. 85 (Pt 10): 2809-14. -
Ashrafi, G.H. et al. (2002) Down-regulation of MHC class I by bovine papillomavirus E5 oncoproteins.
Oncogene. 21: 248-59. -
Suzuki, T. et al. (2003) Evaluation of the delta subunit of bovine adaptor protein complex 3 as a receptor for bovine leukaemia virus.
J Gen Virol. 84 (Pt 5): 1309-16. -
Stephens SA & Howard CJ (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
Parasitology. 124 (Pt 5): 485-93. -
Toye, P.G. et al. (1990) Transfection into mouse L cells of genes encoding two serologically and functionally distinct bovine class I MHC molecules from a MHC-homozygous animal: evidence for a second class I locus in cattle.
Immunology. 70: 20-6. -
Marchetti, B. et al. (2002) The bovine papillomavirus oncoprotein E5 retains MHC class I molecules in the Golgi apparatus and prevents their transport to the cell surface.
Oncogene. 21:7808-16 -
Bainbridge, DR. et al. (2001) Increased expression of major histocompatibility complex (MHC) class I transplantation antigens in bovine trophoblast cells before fusion with maternal cells.
Reproduction.122: 907-13. -
Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
Immunology. 108: 55-61. -
Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
PLoS One. 11 (9): e0162571.
