MHC Class I Monomorphic antibody | IL-A88

Mouse anti Bovine MHC Class I Monomorphic

Product Type
Monoclonal Antibody
Clone
IL-A88
Isotype
IgG2a
Specificity
MHC Class I Monomorphic

Product Code Applications Pack Size List Price Your Price Qty
MCA2444GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Bovine MHC Class I Monomorphic antibody, clone IL-A88 recognizes a monomorphic determinant within the heavy chain of bovine MHC Class I.

Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This antibody immunoprecipitates a band of approximately 45 kDa under reducing conditions.

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for MHC Class I Monomorphic antibody

  1. Ambagala, A.P. et al. (2000) An early pseudorabies virus protein down-regulates porcine MHC class I expression by inhibition of transporter associated with antigen processing (TAP).
    J Immunol. 164 (1): 93-9.
  2. Daubenberger CA et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parva.
    Infect Immun. 67 (5): 2241-9.
  3. Araibi, E.H. et al. (2004) Downregulation of major histocompatibility complex class I in bovine papillomas.
    J Gen Virol. 85 (Pt 10): 2809-14.
  4. Ashrafi, G.H. et al. (2002) Down-regulation of MHC class I by bovine papillomavirus E5 oncoproteins.
    Oncogene. 21: 248-59.
  5. Suzuki, T. et al. (2003) Evaluation of the delta subunit of bovine adaptor protein complex 3 as a receptor for bovine leukaemia virus.
    J Gen Virol. 84 (Pt 5): 1309-16.
  6. Stephens SA & Howard CJ (2002) Infection and transformation of dendritic cells from bovine afferent lymph by Theileria annulata.
    Parasitology. 124 (Pt 5): 485-93.
  7. Toye, P.G. et al. (1990) Transfection into mouse L cells of genes encoding two serologically and functionally distinct bovine class I MHC molecules from a MHC-homozygous animal: evidence for a second class I locus in cattle.
    Immunology. 70: 20-6.
  8. Marchetti, B. et al. (2002) The bovine papillomavirus oncoprotein E5 retains MHC class I molecules in the Golgi apparatus and prevents their transport to the cell surface.
    Oncogene. 21:7808-16
  9. View The Latest Product References
  10. Bainbridge, DR. et al. (2001) Increased expression of major histocompatibility complex (MHC) class I transplantation antigens in bovine trophoblast cells before fusion with maternal cells.
    Reproduction.122: 907-13.
  11. Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro.
    Immunology. 108: 55-61.
  12. Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
    PLoS One. 11 (9): e0162571.
  13. Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
    Comp Immunol Microbiol Infect Dis. 68: 101388.
  14. Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
    Ticks Tick Borne Dis. 11 (3): 101365.

Flow Cytometry

Functional Assays

UniProt
Q30289
View more products with MHC CLASS I specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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